Abstract
Disporopsis pernyi (Hua) Diels, which belongs to genus Disporopsis, has been widely used for the treatment of abnormal sweating, chronic cough, and so forth. An ultra-performance liquid chromatography (UPLC) analysis was developed for the determination of rutin, luteolin, quercetin, and betulinic acid in Disporopsis pernyi (Hua) Diels roots. UPLC analysis was conducted by using a Shim-pack XR-ODS column with gradient elution with the mobile phase of acetonitrile and water containing 0.1% formic acid and with a flow rate of 0.2 mL/min, detected at 210, 254, and 280 nm. The method was precise, with relative standard deviation < 2.0%. The recoveries for the four components in Disporopsis pernyi (Hua) Diels were between 98.5 and 100.9%. The average contents of rutin, luteolin, quercetin, and betulinic acid in roots were 5.63, 2.51, 3.87, and 2.41 μg/g, respectively. The method was accurate and reproducible and it can provide a quantitative basis for quality control of Disporopsis pernyi (Hua) Diels.
Highlights
Disporopsis pernyi (Hua) Diels, which belongs to Disporopsis genus of Liliaceae family, mainly grows in South Asia such as Vietnam, Laos, Thailand, and Yangtze river basin of China
Study suggests that a total of 4 polyphenolic compounds are found in extract including rutin, luteolin, quercetin, and betulinic acid, and the phenolic compounds contribute significantly to the antioxidant and antimicrobial activities [2, 3]
The rutin, luteolin, quercetin, and betulinic acid were purchased from the National Institute for the Control of Pharmaceutical and Biological Products in China
Summary
Disporopsis pernyi (Hua) Diels, which belongs to Disporopsis genus of Liliaceae family, mainly grows in South Asia such as Vietnam, Laos, Thailand, and Yangtze river basin of China It was a well-known traditional Chinese medicine which has been widely used for the treatment of abnormal sweating, chronic cough, women postpartum weakness and irregular menstrual cycle, and so forth [1]. Study suggests that a total of 4 polyphenolic compounds are found in extract including rutin, luteolin, quercetin, and betulinic acid, and the phenolic compounds contribute significantly to the antioxidant and antimicrobial activities [2, 3]. Rutin has both antihypertensive effect and antidiabetes effect [4, 5]. Recent study shows that it has the inhibition effects of cerebroma and leukocythemia [10, 11]
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