Simultaneous Determination Of Cetirizine Research Articles

Synchronous Fluorescence as a Green and Selective Method for the Simultaneous Determination of Cetirizine and Azelastine in Aqueous Humor

A green, simple, quick and economical method is implemented for the first time for the simultaneous estimation of cetirizine (CTZ) and azelastine (AZE) as co-administered eye drops. The method relies on synchronous spectrofluorimetry with ∆λ = 60 nm. Cetirizine can be estimated at 231 nm and AZE can be measured at 294 nm, each at the other’s zero crossing point. All factors affecting the method were studied and properly optimized. Good correlation was obtained in the range of 0.1–2 µg mL−1 for both drugs. The limits of detection were 0.014 and 0.010 µg mL−1 and limits of quantitation were 0.043 and 0.029 µg mL−1 for CTZ and AZE, respectively. Moreover, ICH guidelines were carried out to validate the adopted method. The method was suitable for the analysis of CTZ and AZE in synthetic mixtures, eye drops and aqueous humor. The mean percentage of recoveries of CTZ and AZE in spiked aqueous humor were 99.83 and 99.37, respectively. Furthermore, Green Analytical Procedure Index (GAPI) and analytical Eco-scale approaches were used to evaluate the greenness of the suggested method.

Application of RP-HPLC method for the simultaneous determination of cetirizine in the presence of quinolones

BackgroundPresent work describes a fast, simple, and sensitive procedure for the simultaneous determination of cetirizine in the presence of quinolones using diclofenac sodium as an internal standard. The present work was designed to analyze these compounds in pharmaceutical and clinical labs being economical for use.ResultsThe mobile phase consisted of the simple composition of methanol, acetonitrile, and water in a ratio of 50:20:30 with a pH adjusted to 3.1 at a flow rate of 1 mL min−1. The UV detection was performed at 225 nm. The linearity was assessed over the range of 2.5–50 μg mL−1 for all drugs. The parameters such as accuracy, precision, linearity (>0.999), and sensitivity were satisfactory.ConclusionThe method was equally applicable for formulation and human serum with recovery values between 95 and 105%. The results of the method were validated statistically according to ICH guidelines.

Simultaneous determination of cetirizine, phenyl propanolamine and nimesulide using third derivative spectrophotometry and high performance liquid chromatography in pharmaceutical preparations

BackgroundThe combination between cetirizine (CET), phenylpropanolamine (PPA) and nimesulide (NMS) under trade name Nemeriv Cp tablet is prescribed for nasal congestion, cold, sneezing, and allergy. Among all published methods for the three drugs; there is no reported method concerning estimation of CTZ, PPA and NMS simultaneously and this motivates us to develop new and simple methods for their assay in pure form and tablet preparations.ResultsTwo new methodologies were described for the simultaneous quantification of cetirizine (CTZ), PPA and NMS. Spectrophotometric procedures relies on measuring the amplitudes of the third derivative curves at 238 nm for CTZ, 218 nm for PPA and 305 nm for NMS. The calibration graphs were rectilinear over the ranges of 8–90 µg/mL for CTZ, 20–100 µg/mL for PPA and 20–200 µg/mL for NMS respectively. Regarding the HPLC method; monolithic column (100 mm × 4.6 mm i.d) was used for the separation. The used mobile phase composed of 0.1 M phosphate buffer and methanol in the ratio of 40:60, v/v at pH 7.0. The analysis was performed using UV detector at 215 nm. Calibration curves showed the linearity over concentration ranges of 5–40, 10–100 and 10–120 µg/mL for CTZ, PPA and NMS.ConclusionApplication of the proposed methods to the laboratory prepared tablets was carried out successfully. The results were compared with those obtained from previously published methods and they were satisfactory.Graphical abstractGraphical abstract represents the chemical structures, representative chromatogram for the HPLC separation of a PPA, b NMS and c CTZ and third derivative absorption spectra of a PPA, b NMS and c CTZ for the spectrophotometric method.

Simultaneous Determination of Cetirizine, Atorvastatin, Simvastatin, Rosuvastatin or Pravastatin in Formulations and Human Serum by RP-HPLC

Present work was designed for the simultaneous determination of cetirizine in presence of atorvastatin, simvastatin, rosuvastatin or pravastatin using RP-HPLC method. The chromatographic separation achieved using mobile phase consisted of acetonitrile: water (75:25), monitored at 235 nm with a pH adjusted to 2.8. The linearity was assessed over the range of 2.5-100 µgmL-1 for all drugs. The applicability of the method was verified by the determination of cetirizine, atorvastatin, simvastatin, rosuvastatin and pravastatin in marketed formulations. The results of the method were validated statistically according to ICH guidelines. The recovery values for all the drugs in formulations were found to be 96-108 %. The parameters such as accuracy, precision, linearity, sensitivity (LOQ=0.01-0.07, LOD=0.003-0.02 µgmL-1) were found to be satisfactory. This method can be successfully used for the determination of these drugs in formulations and can be a useful tool for the routine analysis in laboratories.

Simultaneous Determination of Cetirizine and Pseudoephedrine Combined in Tablet Dosage Form by High Performance Liquid Chromatography

This study develops and validates an efficient, sensitive and simple method for the simultaneous determination of cetirizinedihydrochloride and pseudoephedrinecombined in tablet dosage form by high performance liquid chromatography (HPLC) with an ultraviolet (UV) detector.The validation of this method was carried out according to ICH and USP guidelines. In this study, the mobile phase used wasacetonitrile: water (530:470 (v/v)) with 200mg sodium heptane sulfonic acid and the pH value was adjusted to 2.5 with sulfuric acid. The limit of detection and quantification for cetirizinedihydrochloride were 0.805 and 2.685 µg/mL,respectively,and the limit of detection and quantification for pseudoephedrinewere17.976 and 59.921 µg/mL, respectively. The linearity was studied in the concentration range of 12.2 and 36.5 µg/mL forcetirizinedihydrochloride and 295.91 and 861.73 µg/mL for pseudoephedrine. The recovered amounts of cetirizinedihydrochloride and pseudoephedrinewere98.2% -102.9% and99.5%- 102.4%, respectively.