High-strength steel composite Y-eccentrically braced frame (Y-HSS-EBF) is a novel structural system. In this study, the spatial substructure hybrid simulation test (SHST) method is used to further study the seismic performance of Y-HSS-EBF. Firstly, based on the cyclic loading tests of two single-story single-span Y-HSS-EBF planar specimens, a finite element model in OpenSees was verified to provide a reference for the numerical substructure analysis model for the later SHST. Then, the SHST was carried out on the OpenFresco test platform. A three-story spatial Y-HSS-EBF model was taken as the prototype, the top story was taken as the experimental substructure, and the remaining two stories were taken as the numerical substructure to be simulated in OpenSees. According to the test results, the validity of the SHST was verified, and the main seismic performance indexes of the SHST model were analyzed. The results show that, the SHST based on the OpenFresco platform has good stability and accuracy, and the results of the SHST agree well with the global numerical model of the structure. Under strong seismic action, the plastic deformation of Y-HSS-EBF mainly occurs in the shear link, and the beam, beam-columns and braces can basically remain in the elastic state, which is conducive to post-earthquake repair.