Simple Method For Production Research Articles

Characterization and Structural Analysis of Alcohol-Fractionated Lignin Biofuels Processed at Ambient Temperature

This study introduces Cold-processed Lignin in (M)ethanol Oil (CLEO/CLiMO), a novel biofuel technology derived from the alcohol-fractionation of lignin at ambient temperatures, offering a sustainable alternative to conventional marine fuels. The production process achieved solid loadings of up to 60 wt% lignin and a volumetric energy density 39% higher than pure alcohols. Lignin concentrations above 30 wt% promoted colloidal stability through the proposed formation of a spanning network of lignin aggregates, associated with a 100-fold increase of viscosity. Additionally, we observed a decrease in the radius of gyration of lignin particles from 2.5-2.7 nm at 30 wt% to 1.1-1.3 nm at 60 wt% following a transition from globular to elongated random coil shaped particles. This was accompanied by a twofold increase in the partial specific volume of lignin, suggesting a reduction in packing efficiency. The study highlights CLEO's potential as a sustainable shipping fuel alternative, combining favorable fuel properties with a simple and scalable production method.

Unraveling the Ultrasonic-Assisted Synthesis of Green-Emitting CsPbBr3@Cs4PbBr6: Reaction Process, Luminescence Property, and Display Application.

The pursuit of stable and highly emissive perovskite materials has garnered increasing attention in optoelectronic applications. Green-emitting CsPbBr3@Cs4PbBr6, as a green-emissive material in the solid-state form, has great potential as a color conversion material for full-color displays. However, it is a challenge to achieve mass preparation under ambient conditions. Meanwhile, the formation mechanism is ambiguous. This study reports a ligand-free and rapid mass synthesis of nanomicrosized CsPbBr3@Cs4PbBr6 solid via an ultrasonic-assisted method. The synthesized CsPbBr3@Cs4PbBr6 solids exhibit uniform morphology, high stability, and solid-state quantum efficiency of approximately 55%. Moreover, the transformation mechanism from Pb-Br complexes in the synthesis process is fully investigated by monitoring the phase and spectral evolution. By employing it as a green emitter, the obtained white light-emitting diode (LED) device shows high performance with a correlation color temperature of 7635 K and a wide color gamut of 123% NTSC. This study offers a low-cost and simple operation mass production method for solid-state perovskite powders with excellent chemical stability. Additionally, it provides new insights into the formation mechanism of highly efficient perovskite materials and promotes their practical applications.

Flexible Graphene-Based Sensors for Electrochemical Analysis of Human Saliva and Sweat

Flexible carbon nanomaterial sensors offer a low-cost, facile fabrication approach toward producing sensors at an industrial scale for electrochemical sensing. Graphene and carbon nanotube (CNT) based sensors have been studied, with various fabrication methods available, including producing high-quality thin film graphene and CNTs via chemical vapor deposition (CVD). However, CVD is a high-temperature vacuum process that is low-yielding and consequently costly, hindering its implementation into single-use test strip sensors. Other methods, such as inkjet or aerosol printing, are more cost-effective as they print graphene inks obtained from liquid-phase exfoliation of graphite, which can be performed using large batch processing techniques. Additionally, the laser-induced graphene (LIG) technique directly converts sp3 carbon found in carbon-rich substrates, such as polyimide, into conductive sp2-hybridized carbon found in graphene through a laser scribing technique. Saliva and sweat offer promising biological fluids for precision health monitoring and diagnostics due to the non-invasive nature of their sampling (no need for a blood draw at a laboratory or a fingerstick at home) and due to the abundance of medically relevant analytes. Saliva can also be used for monitoring infections such as COVID-19 infection. Herein, we introduce graphene-based flexible sensors fabricated using scalable manufacturing based on aerosol jet printing (AJP) using custom-formulated graphene inks and laser-induced graphene via direct writing. We report a label-free, high-sensitivity, and rapid-response-time graphene-based electrochemical immunosensor for quantitatively detecting the SARS-CoV-2 Spike Receptor-Binding Domain (RBD) as well as SARS-CoV-2 Spike S1 protein. We demonstrate LIG-based electrochemical sensors for real-time monitoring of glucose, lactate, and sodium in sweat, and potassium and lactate in saliva. Different electrochemical methods are used, including amperometric, potentiometric, coulometric, and impedimetric, to quantify the analytes. All these electrochemical sensors were validated in human saliva and sweat and demonstrated to have limits of detection and linear sensing ranges that are relevant to their sensing applications. Flexibility studies were also performed to evaluate the ability of the sensors to operate under stresses caused by bending, such as those that would be encountered by skin and implanted oral sensors. Results demonstrate the utility of AJP-graphene and LIG for rapid and sensitive measurement of biological analytes and pathogen infection as simple methods for scalable production of non-invasive salivary and perspiration (sweat) health sensors.

Highly stable carbon-coated nZVI composite Fe0@RF-C for efficient degradation of emerging contaminants

Nanoscale zerovalent iron (nZVI) has garnered significant attention as an efficient advanced oxidation activator, but its practical application is hindered by aggregation and oxidation. Coating nZVI with carbon can effectively addresses these issues. A simple and scalable production method for carbon-coated nZVI composite is highly desirable. The anti-oxidation and catalytic performance of carbon-coated nZVI composite merit in-depth research. In this study, a highly stable carbon-coated core-shell nZVI composite (Fe0@RF-C) was successfully prepared using a simple method combining phenolic resin embedding and carbothermal reduction. Fe0@RF-C was employed as a heterogeneous persulfate (PS) activator for degrading 2,4-dihydroxybenzophenone (BP-1), an emerging contaminant. Compared to commercial nZVI, Fe0@RF-C exhibited superior PS activation performance and oxidation resistance. Nearly 95% of BP-1 was removed within 10 min in the Fe0@RF-C/PS system. The carbon layer promotes the enrichment of BP-1 and accelerates its degradation through singlet oxygen oxidation and direct electron transfer processes. This study provides a straightforward approach for designing highly stable carbon-coated nZVI composite and elucidates the enhanced catalytic performance mechanism by carbon layers.

Anchoring Ni(OH)2-CeOx Heterostructure on FeOOH-Modified Nickel-Mesh for Efficient Alkaline Water-Splitting Performance with Improved Stability under Quasi-Industrial Conditions.

Developing low-cost and industrially viable electrode materials for efficient water-splitting performance and constructing intrinsically active materials with abundant active sites is still challenging. In this study, a self-supported porous network Ni(OH)2-CeOx heterostructure layer on a FeOOH-modified Ni-mesh (NiCe/Fe@NM) electrode is successfully prepared by a facile, scalable two-electrode electrodeposition strategy for overall alkaline water splitting. The optimized NiCe0.05/Fe@NM catalyst reaches a current density of 100 mA cm-2 at an overpotential of 163 and 262mV for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER), respectively, in 1.0m KOH with excellent stability. Additionally, NiCe0.05/Fe@NM demonstrates exceptional HER performance in alkaline seawater, requiring only 148mV overpotential at 100mA cm-2. Under real water splitting conditions, NiCe0.05/Fe@NM requires only 1.701V to achieve 100mA cm-2 with robust stability over 1000h in an alkaline medium. The remarkable water-splitting performance and stability of the NiCe0.05/Fe@NM catalyst result from a synergistic combination of factors, including well-optimized surface and electronic structures facilitated by an optimal Ce ratio, rapid reaction kinetics, a superhydrophilic/superaerophobic interface, and enhanced intrinsic catalytic activity. This study presents a simple two-electrode electrodeposition method for the scalable production of self-supported electrocatalysts, paving the way for their practical application in industrial water-splitting processes.

Recombinant human enamelin produced in Escherichia coli promotes mineralization in vitro

BackgroundEnamelin is an enamel matrix protein that plays an essential role in the formation of enamel, the most mineralized tissue in the human body. Previous studies using animal models and proteins from natural sources point to a key role of enamelin in promoting mineralization events during enamel formation. However, natural sources of enamelin are scarce and with the current study we therefore aimed to establish a simple microbial production method for recombinant human enamelin to support its use as a mineralization agent.ResultsIn the study the 32 kDa fragment of human enamelin was successfully expressed in Escherichia coli and could be obtained using immobilized metal ion affinity chromatography purification (IMAC), dialysis, and lyophilization. This workflow resulted in a yield of approximately 10 mg enamelin per liter culture. Optimal conditions for IMAC purification were obtained using Ni2+ as the metal ion, and when including 30 mM imidazole during binding and washing steps. Furthermore, in vitro mineralization assays demonstrated that the recombinant enamelin could promote calcium phosphate mineralization at a concentration of 0.5 mg/ml.ConclusionsThese findings address the scarcity of enamelin by facilitating its accessibility for further investigations into the mechanism of enamel formation and open new avenues for developing enamel-inspired mineralized biomaterials.

Ready-to-Use Supplementary-Food Biscuit Production with Low-Cost Ingredients for Malnourished Children in Sub-Saharan Africa.

In Africa, the number of children under 5 years old who suffer from stunting and wasting are, respectively, 61.4 and 12.1 million, and to manage situations like these, emergency food products like RUTF and RUSF (ready-to-use therapeutic/supplementary food) are very useful. The aim of this study was to develop an RUSF biscuit using the low-cost food resources usually present in Sub-Saharan Africa (Burundi and the DRCongo in our case study); we conducted chemical characterization, nutritional evaluation, and a stability trial simulating the usual storage conditions in a rural context to demonstrate that RUSF can be functional also using low-cost ingredients and a simple method of production. The obtained recipes showed good potential in supplying protein integration-17.81% (BUR) and 16.77% (CON) (% as food) were the protein contents-and the protein digestibility values were very high (BUR: 91.72%; CON: 92.01%). Moreover, 30% of the daily requirement was achieved with less than 50 g of both recipes in all the considered ages. Finally, a good shelf-life was demonstrated during the 35-day testing period at 30 °C, considering moisture, texture, and lipid oxidation evolution. Recipes like these, with appropriate changes, could be very useful in all contexts where child malnutrition is a serious problem.

Preparation of mesophase-pitch-based graphite foams at atmospheric pressure

Mesophase-pitch-based graphite foams have attracted increasing attention because of their broad applications in thermal management, catalysis and so on. However, the common approach of using the high-pressure method for fabricating graphite foams is complex and risky. Herein, this research reports a simple and relatively safe technique for preparation of mesophase-pitch-based graphite foams, which is conducted under atmospheric pressure. Through foaming of mesophase pitch in the presence of epoxy resin at 700°C under atmospheric pressure and graphitization at 3000°C, graphite foam is fabricated. The obtained graphite foam is highly graphitic and has an open pore structure, the interlayer spacing of the (002) plane of which is about 0.3366 nm. Its density is 0.52 g/cm3 with a porosity of 73.5%. The properties of graphite foams can be tailored by modifying pitch particle sizes and foaming temperatures. With an increase in pitch particle size from <25 to 75–150 μm or in foaming temperature from 400 to 850°C, the pore sizes vary from 10–25 to 35–90 μm, and their bulk densities change from 0.50 to 1.11 g/cm3. Also, a foaming mechanism is proposed wherein the residue of epoxy resin can form a framework during heating, in which the pitch softens and decomposes, forming foam. This study suggests a relatively simple and safe method for production of graphite foams from mesophase pitch.

Poly-γ-glutamic acid overproduction of Bacillus licheniformis ATCC 9945a by developing a novel optimum culture medium and glutamate pulse feeding using different experimental design approaches.

The commercial production of multifunctional, biocompatible, and biodegradable biopolymers such as poly-γ-glutamic acid via microbial fermentation requires the development of simple and cheap methods for mass production. This study optimized the poly-γ-glutamic acid production of Bacillus licheniformis ATCC 9945a in several steps. At first, the most critical components of the culture medium, including l-glutamic acid, citric acid, and glycerol, were selected by screening nine factors through the Plackett-Burman experimental design and then were optimized using the response surface method and the central composite design algorithm. Under optimal conditions, the production of poly-γ-glutamic acid increased by more than 4.2 times from 11.2 to 47.2g/L. This is one of the highest production rates of this strain in submerged batch fermentation reported so far using the optimized medium compared to the conventional base medium. A novel and efficient sudden pulse feeding strategy (achieved by a novel one-factorial statistical technique) of l-glutamic acid to the optimized medium increased biopolymer production from 47.2 to 66.1g/L, the highest value reported in published literature with this strain. This simple, reproducible, and cheap fermentation process can considerably enhance the commercial applications of the poly-γ-glutamic acid synthesized by B. licheniformis ATCC 9945a .

Photoinduced growth of the crystalline phase of tellurium on a 1T′-MoTe2 matrix

Due to the growing demand for miniaturization and energy efficiency in modern electronic devices, there is a renewed interest for optoelectronic memories and sensors based on 2D materials. In particular, the molybdenum ditelluride (MoTe2) is one of the most promising materials for applications in nonvolatile phase-change memory devices, as its properties can be controlled by visible-light illumination. Among the several ways to synthesize MoTe2, the molybdenum oxide tellurization through isothermal close space sublimation (CSS) annealing in gas atmosphere is a simple and low-cost effective method for large-scale production of devices based on this layered material. Therefore, the understanding of the physical properties of MoTe2 thin films produced by this technique is crucial for future applications. Surprisingly, our results indicate that there is a photoinduced growth of the crystalline phase of tellurium on the 1T′-MoTe2 matrix even when the power density of the laser is low. From Raman spectroscopy investigations, we were able to show that nanometer-sized tellurium crystallites work as seed sites for the photocrystallization of tellurium. By assuming that the overall crystallization process is described by a kinetic approach that is based on the Kolmogorov–Johnson–Mehl–Avrami theory, our results indicate that the process is governed by an anisotropic organization of the tellurium atoms in helical structures during the crystal growth.

Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors.

Acidovorax avenae subsp. avenae (Aaa) is the causal agent of red stripe in sugarcane, a disease characterized by two forms: leaf stripe and top rot. Despite the importance of this disease, little is known about Aaa virulence factors (VFs) and their function in the infection process. Among the different array of VFs exerted by phytopathogenic bacteria, exopolysaccharides (EPSs) often confer a survival advantage by protecting the cell against abiotic and biotic stresses, including host defensive factors. They are also main components of the extracellular matrix involved in cell-cell recognition, surface adhesion, and biofilm formation. EPS composition and properties have been well studied for some plant pathogenic bacteria; nevertheless, there is no knowledge about Aaa-EPS. In this work, we describe a simple and reliable method for EPS production, precipitation, and quantification based on cold precipitation after ethanol addition, which will allow to study EPS characteristics of different Aaa strains and to evaluate the association among EPS (e.g., amount, composition, viscosity) and Aaa pathogenicity.

Unprecedented glucose production using auto-catalytic hydrothermal hydrolysis of lignocellulosic biomass with no catalyst addition

We propose a novel and simple glucose production method involving autocatalytic hydrothermal hydrolysis of biomass without catalyst additives. This method consists of air-oxidation at a relatively lower temperature than that of the conventional method and subsequent hydrothermal hydrolysis. Japanese cedar (indigestible softwood) was initially air–oxidized by heating at 180–200 °C for 3 h. This induced the decomposition of a small portion of cellulose in the cedar sample, although 93.4 % of cellulose remained at 200 °C. The functional acidic groups (phenol, lactonic, and carboxy groups) specifically increased during the initial air–oxidation. Following this, the acidic groups autocatalytically accelerated hydrothermal cellulose hydrolysis, resulting in a maximum glucose yield of 139.6 mg/g raw material at a severity factor (R0) of 3.2 × 104.

Processing of new efficient Cr1-xNaxO3 catalysts for sodium borohydride methanolysis

This study reports the development of a novel Cr1-xNaxO3 catalyst using a simple cost-effective method for hydrogen production from NaBH4 methanolysis. The properties of the nanocomposites were investigated by employing XRD, FTIR, SEM and XPS characterization techniques. The XRD diffraction peaks confirmed the rhombohedral crystal structure of Cr1.xNaxO3 nanoparticles. The average crystal size of these nanoparticles was found to be ranging from 25.0 to 20 nm. FTIR analysis showed the characteristic absorption bands of Cr1.xNaxO3 nanoparticles. The SEM images of Cr2O3 and Cr1.7Na0.3O3 demonstrated that the nanoparticles are irregular in shape, while Cr1.4Na0.6O3 has a porous surface texture. XPS spectra of Cr2O3, Cr1.7Na0.3O3 and Cr1.4Na0.6O3 confirmed the presence of chromium, oxygen, and sodium in the samples. The optical band gaps of Cr2O3 Cr1.7Na0.3O3 and Cr1.4Na0.6O3 nanoparticles were approximately 3.28 eV, 1.61 and 0.81 eV, respectively. Cr1.4Na0.6O3 exhibited the most significant level of activity in terms of hydrogen production of 19144 mL/g.min.

Facile synthesis of ultrathin carbon nanosheets from waste cellulose

Ultrathin carbon nanosheets were fabricated using renewable carbon sources. Cellulose, an important component in the food industry, was processed to form a food byproduct and used to synthesize carbon nanosheets. Both bacterial and nonbacterial cellulose from kombucha byproducts and apple pomace, respectively, were processed via purification and pyrolysis. An inert argon atmosphere and elevated temperatures of 600 °C–800 °C for 20 min were maintained during pyrolysis. Under these conditions, the apple pomace produced a higher yield of nanosheets than the kombucha byproduct. The nanosheets with the thickness of 4 nm were characterized using different spectroscopic techniques such as Fourier transform infrared spectroscopy and Raman spectroscopy as well as microscopic techniques such as scanning electron microscopy and atomic force microscopy. This sustainable, simple, and green method of carbon nanosheet production is a promising alternative to conventional methods of production.

Addressable and stable physically unclonable functions based on cross-linked poly(2-vinylpyridine)

Counterfeiting poses a significant threat to global trade and public health. Effectively combating counterfeiting involves benefiting from stochastic physical processes to build physically unclonable functions (PUFs). Polymers, as low-cost materials with a wide range of functionalities, hold huge potential for PUF applications. In this context, while there are ongoing applications and studies related to PUF systems today, the development of PUF systems that provide high stochastic characteristics and robustness through simple and low-cost production methods remains critically important. In this regard, we propose stable and addressable polymer PUFs, utilizing a cross-linkable poly(2-vinylpyridine) (P2VP). The Rayleigh instability phenomenon occurring in electrohydrodynamic processes is used to fabricate randomized polymeric features. A brief thermal annealing process enhances adhesion with the substrate, while UV-ozone treatment cross-links P2VP. When applied through stencil masks, UV-ozone treatment results in localized cross-linking, yielding addressable randomized features. Structural and chemical analysis is employed to examine the cross-linked polymer features. Adjusting the conditions of electrospraying and cross-linking leads to PUFs with outstanding stability against solvents, oxygen plasma, and thermal heating. The randomized response of polymer features is evaluated through various statistical criteria. Feature matching algorithms enable direct authentication of images captured under different rotations and lighting conditions. This study demonstrates a versatile strategy for creating polymer-based PUFs with remarkable stability and addressability, enabled by a cross-linkable polymer system.

Development of novel PVA-TEOS-MnO2 and its PANI incorporated flexible membrane electrodes and evaluation of their supercapacitor performance

The properties of tetraethyl orthosilicate (TEOS) crosslinked poly (vinyl alcohol) (PVA) membranes were altered by introducing MnO2 nanoparticles through the chemical oxidation of KMnO4. The resulting PVA-TEOS-MnO2-4 membrane was modified by in-situ polymerization of aniline monomer in the membrane matrix, and obtained the PVA-TEOS-MnO2-PANI nanocomposite flexible membrane electrode. After performing the physicochemical studies, the flexible membrane electrodes were subjected to cyclic voltammetry, galvanostatic charge-discharge and electrochemical impedance spectroscopy. The PVA-TEOS-MnO2-PANI electrode membrane exhibited specific capacitance as high as 550.23 F g−1 at a current density of 0.1 A g−1. The specific capacitance and energy density of PVA-TEOS-MnO2-PANI were found to be higher than PVA-TEOS-MnO2-4 and its cycle-life stability was retained more than 90 % of its initial capacitance even after 1000 cycles. Based on the results, it is concluded that the PVA-TEOS-MnO2-PANI nanocomposite flexible membrane electrode could be a potential candidate for the development of flexible supercapacitor devices on large-scale applications. We further fabricated a micro-supercapacitor device and measured its specific capacitance, energy density and power density at a current density of 5.0 mA g−1, and were respectively found to be 59 F g−1, 7.42 Wh kg−1, and 65.89 W kg−1. In addition, the process of fabrication used in this study offers a simple method for the mass production of flexible and light-weight micro-supercapacitor devices.

Enzymatic and selective production of alkyl α-d-glucopyranosides by the α-glucosyl transfer enzyme derived from Xanthomonas campestris WU-9701

Several alkyl glucosides exhibit various bioactivities. 1-Octyl β-d-glucopyranoside produced by organic synthesis is used as a nonionic surfactant. However, no convenient method has been developed for the selective production of alkyl α-glucosides (α-AGs), such as 1-octyl α-d-glucopyranoside (α-OG). Therefore, we developed a simple method for selective production of α-AGs using the glucosyl transfer enzyme XgtA, (E.C. 3.2.1.20), derived from Xanthomonas campestris WU-9701. When 0.80M alkyl alcohol and 2.5 units XgtA were incubated in 2.0mL of 30mM HEPES-NaOH buffer (pH 8.0) containing 1.2M maltose at 45°C, a specific α-AG corresponding to each alkyl alcohol (C2-C10) was detected. Under the standard conditions, we examined the selective production of α-OG from 1-octanol and maltose using XgtA. The reaction product was isolated and identified as α-OG via 1H nuclear magnetic resonance and nuclear overhauser effect spectroscopy analyses. No other glucosylated products, such as maltotriose, were detected in the reaction mixture. Under the standard conditions at 45°C for 96h, 243mM α-OG (71g/L) was produced in one batch production. Moreover, the addition of glucose isomerase to the reaction mixture decreased the concentration of glucose released via the reaction and increased the amount of α-OG produced; 359mM α-OG (105g/L) was maximally produced at 96h. In conclusion, this study demonstrates the selective production of α-AGs using a simple enzymatic reaction, and XgtA has the potential to selectively produce various α-AGs.

Synthesis of length-tunable DNA carriers for nanopore sensing.

Molecular carriers represent an increasingly common strategy in the field of nanopore sensing to use secondary molecules to selectively report on the presence of target analytes in solution, allowing for sensitive assays of otherwise hard-to-detect molecules such as small, weakly-charged proteins. However, existing carrier designs can often introduce drawbacks to nanopore experiments including higher levels of cost/complexity and carrier-pore interactions that lead to ambiguous signals and elevated clogging rates. In this work, we present a simple method of carrier production based on sticky-ended DNA molecules that emphasizes ease-of-synthesis and compatibility with nanopore sensing and analysis. In particular, our method incorporates the ability to flexibly control the length of the DNA carriers produced, enhancing the multiplexing potential of this carrier system through the separable nanopore signals they could generate for distinct targets. A proof-of-concept nanopore experiment is also presented, involving carriers produced by our method with multiple lengths and attached to DNA nanostructure targets, in order to validate the capabilities of the system. As the breadth of applications for nanopore sensors continues to expand, the availability of tools such as those presented here to help translate the outcomes of these applications into robust nanopore signals will be of major importance.

Effect of Shape Tool Parameters on the Performance of Cold Circular Three-Dimensional Pilger Cross-Section Roll Forming

Compared with other forming processes, Pilger cold rolling exhibits unique process characteristics and a simple production method, making it highly advantageous in terms of high-precision, high-strength, and poor-plasticity alloys. Among them, the design parameters of the rolling mill play a significant role in the rolling results, with the key design parameters being the hole opening angle θ and the hole gap ΔK. In this study, a numerical simulation model for cold rolling an AZ31B magnesium alloy with variable cross-section three-roll Pilger cold rolling was established, and finite element simulation analysis was employed to obtain the comprehensive performance impact law of key design parameters on the cold-rolled AZ31B magnesium alloy. It can be concluded that when the hole opening angle is 10° and the hole gap is 1.2 mm, the equivalent stress and equivalent plastic strain of the billet reach a minimum, and the surface precision is excellent.

Получение облепихового масла экстракцией жома плодов облепихи сверхкритическим диоксидом углерода с последующим центрифугированием

A simple method for production of buckthorn buckthorn oil (Hippophae rhamnoides) with a total carotenoid content sufficient to meet the pharmacopoeial requirements for sea buckthorn oil used as a wound healing agent is proposed. The method is based on the extraction of dry pulp of sea buckthorn fruit with supercritical carbon dioxide at high pressures (50 MPa) to achieve a high content of carotenoids in the oil, followed by centrifugation to separate ballast solid wax fractions. Chromatographic and photometric analysis of the chemical composition of the oil confirm the prospects of this approach to obtaining sea buckthorn oil.