Significant Synergistic Effect Research Articles

Thermogravimetric Assessment and Differential Thermal Analysis of Blended Fuels of Coal, Biomass and Oil Sludge

The coupled combustion of biomass and organic solid wastes including oil sludge has attracted much attention. Although the optimal mixing ratio of different coal types and biomass has been extensively studied, little attention has been paid to oil sludge that has undergone co-combustion. In this study, the combustion characteristics of blended fuel for coal, biomass and oil sludge under different mixing ratios are studied via a thermogravimetric test and differential thermal analysis. Kinetic analysis of tri-fuel is performed using the Flynn–Wall–Ozawa (FWO) and Dolye methods. The results show that the bituminous coal combustion process mainly involves the combustion of fixed carbon (236.0–382.0 °C). Wood pellet combustion (383.0–610.0 °C) has two processes involving the combustion of compound carbon and fixed carbon. Blending wood pellets effectively enhances combustion efficiency. Wood pellets from Korla (KOL) have the most obvious effect on reducing the ignition temperature. The blending combustion of bituminous coal (SC), wood pellets from Hutubi (HTB) and oil sludge (OS) have significant synergistic effects. As the OS mixing ratio increases from 10% to 20% with 45% HTB, Ti and Th decrease from 354.9 and 514.3 °C to 269.8 and 452.7 °C, respectively. In addition, f(α) is [−ln(1 − α)]2 for tri-fuel in most mixing ratios when α < 0.5, while f(α) becomes [−ln(1 − α)]3 at α > 0.5. At a high-HTB-level mixing ratio, increasing the OS content causes a decrease in activation energy to 35.87 kJ mol−1. The moderate blending of oil sludge improves the pre-finger factor and the combustion performance.

Synergistic antibacterial action of the iron complex and ampicillin against Staphylococcus aureus

ObjectivesResistance to antibiotics among bacteria of clinical importance, including Staphylococcus aureus, is a serious problem worldwide and the search for alternatives is needed. Some metal complexes have antibacterial properties and when combined with antibiotics, they may increase bacterial sensitivity to antimicrobials. In this study, we synthesized the iron complex and tested it in combination with ampicillin (Fe16 + AMP) against S. aureus.MethodsAn iron complex (Fe16) was synthesized and characterized using spectroscopy methods. Confirmation of the synergistic effect between the iron complex (Fe16) and ampicillin (AMP) was performed using ζ–potential, infrared spectra and FICI index calculated from the minimum inhibitory concentration (MIC) from the checkerboard assay. Cytotoxic properties of combination Fe16 + AMP was evaluated on eukaryotic cell line. Impact of combination Fe16 + AMP on chosen genes of S. aureus were performed by Quantitative Real-Time PCR.ResultsThe MIC of Fe16 + AMP was significantly lower than that of AMP and Fe16 alone. Furthermore, the infrared spectroscopy revealed the change in the ζ–potential of Fe16 + AMP. We demonstrated the ability of Fe16 + AMP to disrupt the bacterial membrane of S. aureus and that likely allowed for better absorption of AMP. In addition, the change in gene expression of bacterial efflux pumps at the sub-inhibitory concentration of AMP suggests an insufficient import of iron into the bacterial cell. At the same time, Fe16 + AMP did not have any cytotoxic effects on keratinocytes.ConclusionsCombined Fe16 + AMP therapy demonstrated significant synergistic and antimicrobial effects against S. aureus. This study supports the potential of combination therapy and further research.

Indirubin derivatives as bifunctional molecules inducing DNA damage and targeting PARP for the treatment of cancer

Based on the facts that significant synergistic effect existed between PARP inhibitors and DNA damage agents and the DNA damage caused by indirubin's derivatives, we herein adopted the strategy to combine the pharmacophores of PARP inhibitors and the unique scaffold of indirubin to design a series of bifunctional molecules inducing DNA damage and targeting PARP. After SAR studies, the most potent compound 12a, encoded as KWWS-12a, exhibited improved inhibitory effect against PARP1 compared with PARP1 inhibitor Olaparib (IC50 = 1.89 nM vs 7.48 nM) and enhanced antiproliferative activities than the combination of Olaparib and indirubin-3′-monoxime towards HCT-116 cells (IC50 = 0.31 μM vs 1.37 μM). In the normal NCM-460 cells, 12a showed low toxicity (IC50 > 60 μM). The mechanism research indicated that 12a could increase the levels of γH2AX concentration dependently, arrest the cell cycle in S phase and induce apoptosis in HCT-116 cells. In vivo experiments showed that 12a displayed more significant antitumor potential than that of the positive controls. Our studies demonstrated that 12a could be a promising candidate for cancer therapy.

FRI530 Immune-Modulation By Rapamycin, Or Mycophenolic Acid, Of Th1 And Th2 Chemokines Secretion In Retro-orbital Cells Of Patients With Graves’ Ophthalmopathy

Abstract Disclosure: G. Elia: None. F. Ragusa: None. S. Paparo: None. V. Mazzi: None. A. Patrizio: None. P. Fallahi: None. A. Antonelli: None. S. Ferrari: None. Objectives: Cytokines and chemokines contribute to the inflammation of Graves’ ophthalmopathy (GO). Indeed cytokines stimulate the T helper (Th)1 and Th2 chemokines release from orbital cells (fibroblasts, preadipocytes and myocytes).We aim to investigate the effects of rapamycin or mycophenolic acid on the secretion of these chemokines in GO orbital cells. Methods: We obtained primary cultures of fibroblasts, preadipocytes and myoblasts from the orbits of GO patients, and we tested increasing concentrations of rapamycin and/or mycophenolic acid evaluating the secretion of the prototype Th1 [chemokine (C-X-C motif) ligand 10 (CXCL10)] and Th2 [chemokine (C-C motif) ligand 2 (CCL2)] chemokines. Results: We observed that CXCL10 secretion in the retro-orbital cells of GO was undetectable in the supernatants, whereas interferon (IFN)-gamma induce its release in a dose-dependently manner. No effect was observed under the tumor necrosis factor (TNF)-alpha stimuli, while the combination of both cytokines had a significant synergistic effect on CXCL10 secretion. As regards the effect on the CCL2 secretion, it was released in low amounts in basal condition; TNF-alpha dose-dependently induced its release, whereas IFN-gamma alone had no effect. The combination of TNF-alpha plus IFN-gamma had a significant synergistic effect on CCL2 secretion. By adding mycophenolic acid, or rapamycin, (in a pharmacological range) at the time of IFN-gamma and TNF-alpha stimulation, we observed a dose-dependently inhibitory effect on the chemokines release in the retro-orbital cells of GO. Furthermore, the combination of mycophenolic acid with rapamycin, have a synergistic inhibitory effect on the release of chemokines. Conclusion: Our data showed an immune-modulating effect of mycophenolic acid or rapamycin on the Th1 and Th2 chemokines secretion, in GO orbital cells. These findings suggest the therapeutic role of these drugs that could obtained, at least in part, through this mechanism. Presentation: Friday, June 16, 2023

Marketing of BRICS innovation clusters in the context of geopolitical instability

The modern stage of society development is characterized by an increased attention to the issues of formation and development of national innovation systems (NIS), which would ensure the integration of various elements of the innovation process, thereby contributing to the build-up of the country's competitive advantages in the global market. Independent development of countries in modern economic conditions is not possible due to the complexity and turbulence of the external economic circuit. In the new geopolitical realities, the fact that past globalization paradigms are no longer having an impact on the viability of the NIS of many countries is becoming evident. At the same time, due to the changing geopolitical landscape, the role of international associations, such as BRICS, is being significantly transformed and they are becoming new centers of influence, including in matters of scientific and technological cooperation. One of the priority conditions for the effective functioning of international S&T cooperation networks and the NIS of each individual country is the creation and maintenance of innovation infrastructure. Already functioning innovation clusters with technology parks, business incubators, accelerators, etc. can become such an infrastructure. Cluster structures are the most effective form of development of international scientific and technological cooperation, which allows to fully realize the potential of countries and structures involved in the interaction, as well as to obtain a significant synergistic and multiplicative effect. The issues of marketing support of innovative cluster associations, which contribute to the successful promotion of clusters in the professional community and target markets, are of particular relevance in this issue.

Does black-box supplier involvement help buyers' product modular and architectural innovation? The moderating role of product modularity

PurposeIn product modular design tasks, especially in the high-tech manufacturing industry, buyers and supplies play distinct roles, which may have different impacts on product architectural and modular innovation. Prior research has tended to view product innovation as a holistic concept, overlooking the importance of this differentiated influence. This study, from a modular design perspective, aims to clarify the impact of black-box supplier involvement on product architectural and modular innovation, as well as the influence of product modularity on these relationships.Design/methodology/approachBased on the theory of product modular design, this study decomposes product innovation into architectural and modular innovation from the perspective of the product internal structure to conduct in-depth theoretical analysis and model construction. A total of 276 valid questionnaires are collected from typical Chinese high-tech manufacturing firms and used to empirically test the constructed theoretical model using multiple hierarchical regression analysis.FindingsThe results show that black-box supplier involvement positively affects modular innovation and takes an inverted U-shape, as moderated by product modularity. However, the impact of black-box supplier involvement on architectural innovation shows contradictory differences at different modularity levels. Under a low level of product modular design, black-box supplier involvement has a negative impact on architectural innovation, but under a moderate level of modular design, it has a positive impact. After the degree of modular design exceeds a certain threshold, the impact gradually weakens.Practical implicationsThe results provide valuable insights for managers, highlighting the need to avoid oversimplifying the innovation impact of black-box suppliers solely based on overall product innovation. Instead, a more accurate assessment of the innovation contributions of both the buyer and supplier should be based on the degree of architectural and modular innovation. Additionally, the findings suggest that managers should consider the alignment between their company's product modular design features and innovation priorities (i.e. modular innovation or architectural innovation) when determining an appropriate supplier collaborative development strategy.Originality/valueThis study not only reveals the different impacts of black-box supplier involvement on architectural and modular innovation, but also proves the significant synergistic innovation effect of the relationship between black-box supplier involvement and product modularization. It constitutes an enriched and deepened exploration in the existing research on supplier involvement in product innovation.

Comprehensive insight into co-pyrolysis of sewage sludge and tobacco stalk: Effects of mixing ratio and pyrolysis conditions on product distribution and characteristics

Co-pyrolysis of sewage sludge (SS) and tobacco stalk (TS) was conducted, where TS are rich in inorganic elements. The effects of the mixing ratio and pyrolysis temperature on pyrolysis products were investigated through the tube furnace system. The components of gas and tar were determined. In addition, the N/S ratio, pore distribution characteristics, surface functional group characteristics, aromatisation characteristics and inorganic element content of char were studied. Results show that the addition of TS to SS has a significant synergistic effect. Particularly, when the ratio of SS to TS is 1:3, the pyrolysis gas yield is the largest. The inorganic elements with a catalytic effect can promote the conversion of char and tar into pyrolysis gas. However, with the increase in pyrolysis temperature, the synergistic effect will gradually weaken. The increase in pyrolysis temperature will promote the development of mesopores in char but will reduce the order degree of the char structure. The addition of TS in SS will further increase the number of mesopores in char, and the obtained char carbon structure is more ordered. When the pyrolysis temperature is 600 °C, the tar yield is the highest, and the higher the mixing ratio of SS, the better the formation of polyaromatic compounds and aliphatic hydrocarbons.

Photosynthetic Living Fiber Fabrication from Algal-Bacterial Consortia with Controlled Spatial Distribution.

Living materials that combine living cells and synthetic matrix materials have become promising research fields in recent years. While multicellular systems present exclusive benefits in developing living materials over single-cell systems, creating artificial multicellular systems can be challenging due to the difficulty in controlling the multicellular assemblies and the complexity of cell-to-cell interactions. Here, we propose a coculture platform capable of isolating and controlling the spatial distribution of algal-bacterial consortia, which can be utilized to construct photosynthetic living fibers. Through coaxial extrusion-based 3D printing, hydrogel fibers containing bacteria or algae can be deposited into designated structures and further processed into materials with precise geometries. In addition, the photosynthetic living fibers demonstrate a significant synergistic catalytic effect resulting from the immobilization of both bacteria and algae, which effectively optimizes sewage treatment for bioremediation purposes. The integration of microbial consortia and 3D printing yields functional living materials with promising applications in biocatalysis, biosensing, and biomedicine. Our approach provides an optimized solution for constructing efficient multicellular systems and opens a new avenue for the development of advanced materials.

Spatial-temporal variations of vegetation coverage and its driving force in Inner Mongolia grassland, China

Inner Mongolia grassland is rich in natural vegetation and mineral resources. Based on Landsat5/7/8 NDVI data, we used pixel binary model to invert vegetation coverage of Inner Mongolia grassland area, investigated the stability, spatial distribution, and future evolution trend of vegetation coverage by using Sen+MK and Hurst index, and analyzed the driving factors of the spatial differentiation of vegetation coverage by the optimal parameters-based geographical detector. The results showed that vegetation coverage of Inner Mongolia grassland showed an increasing trend from 2006 to 2020, and the overall spatial pattern was high in the east and low in the west, mainly with great fluctuation. The regions with slight or obvious improvement characteristics (64.8%) were much more than those with slight or severe degradation characteristics (23.2%). Compared with that in the past 15 years, the proportion of degraded vegetation in the future is expected to increase to 36.6%. The central part of Xilin Gol League and Wulanqab in the central grassland area, the western part of Hulunbuir and Erdos in the eastern grassland area, and Wuhai in the western grassland area were at the risk of degradation, which should be paid more attention. Precipitation was the dominant factor of spatial differentiation in Inner Mongolia grassland, while soil type, land use, and air temperature had the most significant synergistic effect.

2,4-Diacetylphloroglucinol (DAPG) derivatives rapidly eradicate methicillin-resistant staphylococcus aureus without resistance development by disrupting membrane

Methicillin-resistant Staphylococcus aureus (MRSA) causes severe public health challenges throughout the world, and the multi-drug resistance (MDR) of MRSA to antibiotics necessitates the development of more effective antibiotics. Natural 2,4-diacetylphloroglucinol (DAPG), produced by Pseudomonas, displays moderate inhibitory activity against MRSA. A series of DAPG derivatives was synthesized and evaluated for their antibacterial activities, and some showed excellent activities (MRSA MIC = 0.5–2 μg/mL). Among these derivatives, 7g demonstrated strong antibacterial activity without resistance development over two months. Mechanistic studies suggest that 7g asserted its activity by targeting bacterial cell membranes. In addition, 7g exhibited significant synergistic antibacterial effects with oxacillin both in vitro and in vivo, with a tendency to eradicate MRSA biofilms. 7g is a promising lead for the treatment of MRSA.

A novel DBD/VUV/PMS process for efficient sulfadiazine degradation in wastewater: Singlet oxygen-dominated nonradical oxidation

In this study, a novel process of dielectric barrier discharge plasma/vacuum ultraviolet/peroxymonosulfate (DBD/VUV/PMS) for the nonradical-dominated degradation of sulfadiazine (SDZ) was investigated. The hybrid system has significant synergistic effects, with 95.5% SDZ and 68.3% TOC removal within 10 min. The activation efficiency of DBD/VUV (69.0%) on PMS via multipath was 2.07 times higher than that of single DBD (33.3%) under alkaline conditions. Electron paramagnetic resonance analyses and trapping experiments showed 1O2 was the primary active substance in the DBD/VUV/PMS process. The predominant role of 1O2 revealed that SDZ removal mainly followed the nonradical reaction pathway, contrary to the previously reported non-thermal plasma (NTP)-based radical-dominated process. Multiple spectroscopy analysis showed the efficient degradation process of SDZ. Unlike the radical attack sites, the SDZ transformation pathway by nonradical 1O2 was probably initiated by an aniline ring site attack based on density functional theory (DFT) calculations and product analyses. The DBD/VUV/PMS process reduced energy consumption by 69% compared to DBD. Finally, the evaluation of ecotoxicity and PMS utilization demonstrated the advantages and application prospects of the DBD/VUV/PMS process. This research developed a new nonradical-dominated pathway for antibiotic degradation by the photo/plasma/persulfate process.

Exploration of the synergistic effect of co-pyrolysis of biomass and waste plastics based on ReaxFF-MD and DFT

Co-pyrolysis of biomass and waste plastics is a strategy to achieve efficient waste management and obtain valuable fuels. In this paper, cellulose (CE) and polyethylene (PE) were chosen as representatives of biomass and plastics to investigate the synergistic effects of the main products during co-pyrolysis. The synergistic mechanisms are clarified at the microscopic level by ReaxFF-MD. Mainly, the ·H and CnHm· fragments from PE fracture combine with the intermediate products from CE cracking to generate additional oil and gas products. By using DFT theory to calculate the reaction energy barrier of the main products in the CE/PE co-pyrolysis process, it is found that the reaction energy barrier of furans and ethylene in the separate pyrolysis process is higher than that in the co-pyrolysis process, indicating that the co-pyrolysis process is more prone to reaction generation, leading to more significant synergistic effects. With the increase of temperature, the positive synergistic effect of product can be enhanced within a certain temperature range. But the synergistic oil yield then decreases with further increase of temperature. The synergistic effect is most significant when the material ratio is 1:1. More oxygenated compounds can be obtained by increasing the percentage of CE while more hydrocarbons can be obtained by increasing the percentage of PE.

Acute anti-inflammatory activity of Artemisia campestris and Citrus aurantium: Effects of essential oil combinations

The search for effective anti-inflammatory agents with minimal side effects using mixture of natural products extracts is an important area of research. The aim of the present study was to investigate the in vivo synergetic effects on the acute anti-inflammatory responses using binary combinations of essential oils at different volume ratios. Two plants were utilized: the aerial parts of Artemisia campestris (A.c) and the fruit peels of Citrus aurantium (C.a). The results demonstrated the existence of interesting synergistic effects for the mixture ratios C.a/A.c = 80/20, 70/30, 60/40 and 50/50 “v/v”. Among these, the most significant synergistic effect, particularly strong synergism, was observed for the 70/30 mixture ratio, with CI values of 0.24, 0.33, and 0.27 for doses of 100, 200, and 400 mg/kg, respectively. However, an antagonist effect was observed for the mixture ratio C.a/A.c = 90/10. The dose reduction index (DRI) analysis revealed that the dose of C.a can be reduced by 7.09-fold, and similarly, the dose of A.c can be reduced by 9.64-fold in the combination, compared to the ED50 values of each essential oil. The favorable DRI (>1) indicates a potential for dose reduction, leading to a reduction in toxicity. The findings support the potential application of these plants extracts in the management of inflammation and highlight the importance of optimizing the mixture ratios to achieve greatest synergistic effects. Moreover, these findings may have significant implications for food and pharmaceutical industries, particularly in terms of economic efficiency and reduced toxicity.

Diclofenac and metformin synergistic dose dependent inhibition of hamster fibrosarcoma, rescued with mebendazole

We examined whether combinig diclofenac and metformin in doses equivalent to human doses would synergize their anticancer activity on fibrosarcoma inoculated to hamsters and in vitro. Rescue experiment was performed to examine whether the prosurvival NF-κB stimulation by mebendazole can reverse anticancer effects of the treatment. BHK-21/C13 cell culture was subcutaneously inoculated to Syrian golden hamsters randomly divided into groups (6 animals per group): 1) untreated control; treated daily with 2) diclofenac; 3) metformin; 4) combinations of diclofenac and metformin at various doses; 5) combination of diclofenac, metformin and mebendazole; 6) mebendazole. Dose response curves were made for diclofenac and metformin combination. Tumor growth kinetics, biophysical, pathological, histological and immunohistochemical characteristics of excised tumors and hamster organs as well as biochemical and hematological blood tests were compared among the groups. Single treatments had no anticancer effects. Diclofenac (60 mg/kg/day) exhibited significant (P < 0.05) synergistic inhibitory effect with metformin (500 mg/kg/day) on all tumor growth parameters, without toxicity and influence on biochemical and hematological blood tests. The same results were obtained with double doses of diclofenac and metformin combination. The addition of mebendazole to the diclofenac and metformin combination rescued tumor expansion. Furthermore, diclofenac with metformin demonstrated antiproliferative effects in hamster fibrosarcoma BHK-21/C13, human lung carcinoma A549 (CCL-185), colon carcinoma HT-29 (HTB-38) and cervical carcinoma HeLa (CCL-2) cell cultures, with markedly lower cytotoxicity in the normal fetal lung MRC-5 cells. In conclusion, diclofenac and metformin combination may be recommended for potential use in oncology, due to synergistic anticancer effect in doses achievable in humans.

Synergistic Co-liquefaction of waste plastic express bags and low-rank coal based on supercritical water-ethanol system: Waste treatment and resource upgrading

Recently, with the ongoing development of the express industry, the consumption of waste plastic express bags has been increasing year by year, which has brought considerable pressure on municipal waste management. At the same time, in China, where coal is the main energy resource, the upgrading of low-rank coal is a more reliable and prospective way. An effective method for waste treatment and resource upgrading by synergistic co-liquefaction of waste plastic express bags and low-rank coal in a supercritical water-ethanol system has been developed. The effects of different experimental factors (temperature, residence time, plastic-coal ratio, water-ethanol ratio) on the conversion ratio and synergistic effect of co-liquefaction were investigated. The results showed that elevated temperature, lower water-ethanol ratio as well as suitable residence time, and plastic-coal ratio of 1:1 were conducive to the conversion ratio. Temperature ≥375 °C and suitable plastic-coal ratio (1:1) had a significant positive synergistic effect on the conversion ratio, whereas the other conditions exhibited no or negative synergistic effect on the conversion ratio. The maximum conversion ratio and positive synergistic efficiency were 85.11% and 12.25% at 425 °C, 60 min, water-ethanol ratio of 1:1, and plastic-coal ratio of 1:1, respectively. It was observed after FT-IR and elemental analysis based on the solid residue that the oxygen-containing functional groups in the residue disappeared, the oxygen content decreased, the carbon content increased, and the higher heating value (HHV) increased significantly, and the residue could be further used (such as gasification or pyrolysis) without the need for landfill. The GC-MS results of the oil products suggested that the oil products contained high content of hydrocarbons and phenols, as well as esters, alcohols, and other compounds. The hydrocarbons and phenols mainly derived from the decomposition of long chains in plastics and the decomposition of aromatic structures in coal, and the hydrocarbon and phenolic contents produced at 400 °C were 27.74% and 32.62%, respectively. Thus, the oil product can be used further as a chemical raw material. It cannot be ignored that the ethanol medium after reaction can also be further recycled by rotary evaporation. The synergistic co-liquefaction of waste plastic express bags and low-rank coal based on supercritical water-ethanol system is a promising method for waste treatment and resource upgrading.

Eugenol works synergistically with colistin against colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae isolates by enhancing membrane permeability.

Colistin is a potent antibiotic for the treatment of carbapenem-resistant Gram-negative bacteria and is considered a last-resort drug. Unfortunately, the incidence of colistin-resistant bacteria isolated from patients is continuously growing due to clinical reuse of colistin. In this study, we found that the combination of colistin and eugenol has a significant synergistic antibacterial effect and reverses the sensitivity of colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae against colistin, as confirmed by checkerboard and time-kill assays. Crystal violet staining and scanning electron microscopy revealed colistin and eugenol's synergistic antibiofilm action. Concerning the synergy mechanism, the results revealed that the combination of eugenol and colistin increases membrane permeability and causes considerable membrane damage, further inhibiting bacteria synergistically. Meanwhile, up to 500 µg/mL of eugenol is non-toxic to RAW 264.7 cells, and the colistin/eugenol combination is also efficacious in vivo, as demonstrated by the Galleria mellonella infection model. Our findings indicate that the colistin/eugenol combination is a viable treatment option for colistin-resistant P. aeruginosa and K. pneumoniae clinical infections. IMPORTANCE Colistin is used as a last resort for severe infections caused by multidrug-resistant Gram-negative bacteria, however, colistin resistance is increasing. As a result, we investigated the synergistic effect of eugenol/colistin combination, and the results revealed significant antibacterial and antibiofilm action. Eugenol may help clinical colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae recover their susceptibility. These findings suggest that combining eugenol and colistin may be a viable treatment option for colistin-resistant pathogen clinical infections.

Has China achieved synergistic reduction of carbon emissions and air pollution? Evidence from 283 Chinese cities

Confronted with the dual pressures of carbon emissions and air pollution, examining their synergistic effects becomes increasingly paramount in China. This study verifies and quantifies the bidirectional synergistic effects between reductions in carbon emissions and air pollutants (SO2 and PM2.5) at the city level. We also analyzed the spatial spillover of this effect among cities, investigating the dynamic evolution, heterogeneity, and underlying mechanisms of both synergistic reduction and increase effects across different time scales. Key findings include: 1) Carbon emission mitigation causes greater synergistic reduction effect than pollution reduction; 2) Carbon emission reduction has a more significant synergistic effect on SO2 than PM2.5; 3) Synergistic effects vary across city types. Cities with higher resource endowments, lower administrative levels, smaller populations, and lower economic status show greater synergistic effects; 4) The synergistic effects demonstrate significant positive spatial spillovers across cities, with local reductions in carbon and sulfur emissions spurring sulfur emission reductions in neighboring regions; 5) Synergistic reduction effects between carbon and air pollutant emissions exhibit temporal delays, while their synergistic increase effects are pronounced in both short and long terms; 6) R&D investment mitigates synergistic increase effects, while the impact of energy efficiency on synergistic reduction effects shows an inverted U-curve relationship. Our findings offer valuable insights for policymakers seeking to simultaneously tackle climate change and environmental pollution more effectively.

Assessing the Efficacy of A Mo2C/Peroxydisulfate System for Tertiary Wastewater Treatment: A Study of Losartan Degradation, E. coli Inactivation, and Synergistic Effects

This work examines the use of pristine Mo2C as an intriguing sodium persulfate (SPS) activator for the degradation of the drug losartan (LOS). Using 500 mg/L Mo2C and 250 mg/L SPS, 500 μg/L LOS was degraded in less than 45 min. LOS decomposition was enhanced in acidic pH, while the apparent kinetic constant decreased with higher LOS concentrations. According to experiments conducted in the presence of scavengers of reactive species, sulfate radicals, hydroxyl radicals, and singlet oxygen participated in LOS oxidation, with the latter being the predominant reactive species. The presence of competitors such as bicarbonate and organic matter reduced the observed efficiency in actual matrices, while, interestingly, the addition of chloride accelerated the degradation rate. The catalyst showed remarkable stability, with complete LOS removal being retained after five sequential experiments. The system was examined for simultaneous LOS decomposition and elimination of Escherichia coli. The presence of E. coli retarded LOS destruction, resulting in only 30% removal after 3 h, while the system was capable of reducing E. coli concentration by 1.23 log. However, in the presence of simulated solar irradiation, E. coli was reduced by almost 4 log and LOS was completely degraded in 45 min, revealing a significant synergistic effect of the solar/Mo2C/SPS system.

Mechanistic Study and Application of Anionic/Cationic Combination Collector ST-8 for the Flotation of Spodumene

The mechanism of anionic/cationic combination collector ST-8 for the efficient separation of spodumene and feldspar was investigated by flotation tests, surface tension measurements, zeta potential measurements and infrared spectroscopy analysis. The flotation results indicated that when the optimum molar ratio of sodium oleate and dodecylamine was 6:1–10:1 and pH = 8.5, the recovery of spodumene reached a maximum of 80% and that of feldspar reached about 24.5%. Zeta potential measurements showed that the negative shift of the kinetic potential on the surface of spodumene was much stronger than that on the surface of feldspar, indicating that the adsorption capacity of the combined collector on spodumene surface and feldspar surface was greatly different. From the surface tension as well as the synergistic parameters, there was a strong mutual attraction between dodecylamine and sodium oleate, and there was a significant synergistic effect between them. Infrared spectroscopy indicated that the combined collector chemisorbed on the mineral surface, and the intensity of the absorption peak after the action of spodumene was much stronger than that after the action of feldspar. The solution chemistry of the combined collector revealed that at pH 8.5, the positively charged ions RNH3+ and (RNH3+)22+ in dodecylamine and the negatively charged RCOO− and (RCOO−)22− ions in oleic acid form highly reactive complexes to interact with the mineral surface by electrostatic gravitation, thus achieving efficient separation of spodumene and feldspar. Finally, from the closed-circuit test results, a flotation index of 6.34% Li2O grade and 88.51% Li2O recovery was obtained.

Investigation of the combined cytotoxicity induced by sodium butyrate and a flavonoid quercetin treatment on MCF-7 breast cancer cells

ABSTRACT Quercetin (QUE) belonging to the flavonoid class is a common phytochemical present in the daily diet of some individuals. Quercetin is an important source of free radical scavengers. This property makes this flavonoid a reliable antioxidant with the following properties: anti-inflammatory, anti-diabetic, antimicrobial and anti-carcinogenic. Sodium butyrate (NaBu) acts as a histone deacetylase inhibitor (HDACi) and is known to regulate apoptosis in cancer cells. Combining natural flavonoids such as QUE with different substances may synergistically enhance their anti-carcinogenic capacity. Thus, the aim of this study was to examine the combined treatment effects of QUE and NaBu in hormone-sensitive breast cancer cells in vitro. MCF-7 breast cancer cells were treated with QUE alone, NaBu alone, as well as QUE and NaBu combined to determine the following: cell proliferation, levels of protein annexin A5 (ANXA5) and reactive oxygen species (ROS), mRNA protein expression, as well as cell and nuclear morphology. Data demonstrated that either QUE or NaBu alone inhibited cell proliferation, and reduced levels protein ANXA5, ROS and mRNA protein expression, The combination of QUE and NaBu produced a significant synergistic inhibitory effect compared to treatment groups of QUE or NaBu alone. In conclusion, our findings showed that the combination treatment of QUE and NaBu may constitute a promising therapeutic approach to breast cancer treatment but this needs further molecular and in vivo investigations