Significant Interaction Research Articles

Age-specific differences in tumour characteristics between screen-detected and non-screen-detected breast cancers in women aged 40-74 at diagnosis in Sweden from 2008 to 2017.

To analyze differences between screen-detected and non-screen-detected invasive breast cancers by tumour characteristics and age at diagnosis in the nationwide population-based mammography screening program in Sweden. Data were retrieved from the National Quality Register for Breast Cancer for 2008-2017. Logistic regression analysis was used to estimate the likelihood for a tumour to be screen-detected by tumour characteristics and age group at diagnosis. In total there were 51,429 invasive breast cancers in the target age group for mammography screening of 40-74 years. Likelihood of screen detection decreased with larger tumour size, lymph node metastases, higher histological grade and distant metastasis. Odds ratios (ORs) for negative oestrogen (ER) and progesterone (PgR) were 0.41 and 0.57; for positive HER2, 0.62; for Ki-67 high versus low, 0.49. Molecular sub-types had OR of 0.56, 0.40 and 0.28, respectively, for luminal B-like, HER2-positive and triple negative versus luminal A-like. Adjusting for tumour size (T), lymph node status (N), age, year and county at diagnosis slightly elevated the ORs. Statistically significant interactions between tumour characteristics and age were found (p < 0.05) except for ER and PgR. The age group 40-49 deviated most from the other age groups. Our study demonstrates that screen-detected invasive breast cancers had more favourable tumour characteristics than non-screen-detected after adjusting for age, year and county of diagnosis, and even after adjusting for T and N. The trend towards favourable tumour characteristics was less pronounced in the 40-49 age group compared to the other age groups, except for ER and PgR.

Neuronal deletion of Gtf2i results in developmental microglial alterations in a mouse model related to Williams syndrome.

Williams syndrome (WS) is a genetic neurodevelopmental disorder caused by a heterozygous microdeletion, characterized by hypersociability and unique neurocognitive abnormalities. Of the deleted genes, GTF2I has been linked to hypersociability in WS. We have recently shown that Gtf2i deletion from forebrain excitatory neurons, referred to as Gtf2i conditional knockout (cKO) mice leads to multi-faceted myelination deficits associated with the social behaviors affected in WS. These deficits were potentially mediated also by microglia, as they present a close relationship with oligodendrocytes. To study the impact of altered myelination, we characterized these mice in terms of microglia over the course of development. In postnatal day 30 (P30) Gtf2i cKO mice, cortical microglia displayed a more ramified state, as compared with wild type (controls). However, postnatal day 4 (P4) microglia exhibited high proliferation rates and an elevated activation state, demonstrating altered properties related to activation and inflammation in Gtf2i cKO mice compared with control. Intriguingly, P4 Gtf2i cKO-derived microglial cells exhibited significantly elevated myelin phagocytosis invitro compared to control mice. Lastly, systemic injection of clemastine to P4 Gtf2i cKO and control mice until P30, led to a significant interaction between genotypes and treatments on the expression levels of the phagocytic marker CD68, and a significant reduction of the macrophage/microglial marker Iba1 transcript levels in the cortex of the Gtf2i cKO treated mice. Our data thus implicate microglia as important players in WS, and that early postnatal manipulation of microglia might be beneficial in treating inflammatory and myelin-related pathologies.

Understanding the complex formation of falstatin; an endogenous macromolecular inhibitor of falcipains

Proteolytic activity constitutes a fundamental process essential for the survival of the malaria parasite and is thus highly regulated. Falstatin, a protease inhibitor of Plasmodium falciparum, tightly regulates the activity of cysteine hemoglobinases, falcipain-2 and 3 (FP2, FP3), by inhibiting FP2 through a single surface exposed loop. However, the multimeric nature of falstatin and its interaction with FP2 remained unexplored. Here we report that the N-terminal falstatin region is highly disordered, and needs chaperone activity (heat-shock protein 70, HSP70) for its folding. Protein-protein interaction assays showed a significant interaction between falstatin and HSP70. Further, characterization of the falstatin multimer through a series of biophysical techniques identified the formation of a falstatin decamer, which was extremely thermostable. Computational analysis of the falstatin decamer showed the presence of five falstatin dimers, with each dimer aligned in a head-to-tail orientation. Further, the falstatin C-terminal region was revealed to be primarily involved in the oligomerization process. Stoichiometric analysis of the FP2-falstatin multimer showed the formation of a heterooligomeric complex in a 1:1 ratio, with the participation of ten subunits of each protein. Taken together, our results report a novel protease-inhibitor complex and strengthens our understanding of the regulatory mechanisms of major plasmodium hemoglobinases.

Greenness, Genetic Predisposition, and Tinnitus.

This study aimed to investigate the association between residential greenness and tinnitus and the potential interaction between greenness and genetic predisposition to tinnitus. The normalized difference vegetation index (NDVI) is used to measure residential greenness. The tinnitus is defined based on self-reported. In the cross-sectional analyses, logistic regression models are used for the baseline sample of the United Kingdom Biobank cohort. In the secondary analysis, a Cox proportional hazard model is used for a subsample of participants who completed the tinnitus questionnaire at follow-up. In the cross-sectional analysis including 106471 participants, higher residential greenness is associated with lower odds of tinnitus for each interquartile range increase in continuous NDVI, with an adjusted odds ratio of 0.97 (95% confidence interval: 0.95 to 0.99) for tinnitus. A similar association is observed in the longitudinal analysis, with an adjusted hazard ratio of 0.92 (95% confidence interval: 0.86 to 0.98) for the association of NDVI increased per interquartile range with incident tinnitus. Moreover, there is a significant interaction between greenness and genetic predisposition to tinnitus (P < 0.05). This study suggested that residential greenness is negatively associated with tinnitus. Greenness and genetic predisposition to tinnitus are found to have a significant interaction.

The perspective of managers on integrating HR practices to increase organizational performance in SME firms: An analysis of the interaction effect

Purpose: The aim of this paper is to clarify the human resource management (HRM) literature in small-to-medium-sized enterprises (SMEs) by empirically identifying possible combinations of HRM practices from the perspective of managers, and analyzing the relationships among them and with organizational performance in order to identify which combinations are most effective.Design/Methodology/Approach: Using the AMO (Ability- Motivation-Opportunity) framework, we ask whether there are significant interactions between AMO-based HRM bundles and, if so, to what degree and by what means they influence organizational performance. To explore this question, we tested a theoretical model using Partial Least Squares-Structural Equation Modeling (PLS-SEM) techniques and compared the interaction and main effects. The research sample comprises SMEs operating in the Basque Region of Spain.Findings: The study confirms the existence of hierarchies among bundles. The practices most strongly related to the SMEs’ performance lie in the bundle of motivation-enhancing practices. The results indicate that motivation-enhancing practices have a positive effect on organizational performance and when they are combined with ability-enhancing practices this effect grows.Research limitations/implications: The cross-sectional nature of the data means that it is not possible to establish clear causal relationships among the variables studied. It would therefore be beneficial in the future to use longitudinal designs and examine cause-effect over time, as well as use samples from other geographic locations.Practical Implications: High-performance HRM practices in the three Ability-Motivation-Opportunity (AMO) dimensions should not automatically be assumed to be complementary in an SME context, as many managers believe. Our results suggest that managers would be well advised to adopt motivational practices, combining them with training, to improve SMEs’ performance.Originality / value: This is one of the few studies to focus on testing the interaction effects among dimensions of Ability-Motivation-Opportunity-enhancing-practices on SMEs’ organizational performance, as observed by managers. It will encourage SME managers to consider more carefully the possible combinations of AMO dimensions and to focus on those combinations most likely to have positive effects.

Catastrophic health expenditures: a disproportionate risk in uninsured ethnic minorities with diabetes

BackgroundChargemaster prices are the list prices that providers and health systems assign to each of their medical services in the US. These charges are often several factors of magnitude higher than those extended to individuals with either private or public insurance, however, these list prices are billed in full to uninsured patients, putting them at increased risk of catastrophic health expenditures (CHE). The objective of this study was to examine the risk of CHE across insurance status, diabetes diagnosis and to examine disparity gaps across race/ethnicity.MethodsWe perform a retrospective observational study on a nationally representative cohort of adult patients from the Medical Expenditure Panel Survey for the years 2002–2017. Using logistic regression models we estimate the risk of CHE across insurance status, diabetes diagnosis and explore disparity gaps across race/ethnicity.ResultsOur fully adjusted results show that the relative odds of having CHE if uninsured is 5.9 (p < 0.01) compared to if insured, and 1.1 (p < 0.01) for patients with a diabetes diagnosis (compared to those without one). We note significant interactions between insurance status and diabetes diagnosis, with uninsured patients with a diabetes diagnosis being 9.5 times (p < 0.01) more likely to experience CHE than insured patients without a diabetes diagnosis. In terms of racial/ethnic disparities, we find that among the uninsured, non-Hispanic blacks are 13% (p < 0.05), and Hispanics 14.2% (p < 0.05), more likely to experience CHE than non-Hispanic whites. Among uninsured patients with diabetes, we further find that Hispanic patients are 39.3% (p < 0.05) more likely to have CHE than non-Hispanic white patients.ConclusionsOur findings indicate that uninsured patients with diabetes are at significantly elevated risks for CHE. These risks are further found to be disproportionately higher among uninsured racial/ethnic minorities, suggesting that CHE may present a channel through which structural economic and health disparities are perpetuated.

In vitro functional analysis and in silico structural modelling of pathogen-secreted polyglycine hydrolases

Polyglycine hydrolases are fungal effectors composed of an N-domain with unique sequence and structure and a C-domain that resembles β-lactamases, with serine protease activity. These secreted fungal proteins cleave Gly-Gly bonds within a polyglycine sequence in corn ChitA chitinase. The polyglycine hydrolase N-domain (PND) function is unknown. In this manuscript we provide evidence that the PND does not directly participate in ChitA cleavage. In vitro analysis of site-directed mutants in conserved residues of the PND of polyglycine hydrolase Es-cmp did not specifically impair protease activity. Furthermore, in silico structural models of three ChitA-bound polyglycine hydrolases created by High Ambiguity Driven protein-protein DOCKing (HADDOCK) did not predict significant interactions between the PND and ChitA. Together these results suggest that the PND has another function. To determine what types of PND-containing proteins exist in nature we performed a computational analysis of Foldseek-identified PND-containing proteins. The analysis showed that proteins with PNDs are present throughout biology as either single domain proteins or fused to accessory domains that are diverse but are usually proteases or kinases.

Ultrafine Particles and Hospital Visits for Chronic Lower Respiratory Diseases in New York State.

Rationale: Exposure to particulate matter is associated with various adverse health outcomes. Ultrafine particles (UFPs; diameter <0.1 μm) are a unique public health challenge because of their size. However, limited studies have examined their impacts on human health, especially across seasons and demographic characteristics. Objectives: To evaluate the effect of UFP exposure on the risk of visiting the emergency department (ED) for a chronic lower respiratory disease (CLRD) in New York State in 2013-2018. Methods: We used a case-crossover design and conditional logistic regression to estimate how UFP exposure led to CLRD-related ED visits. GEOS-Chem Advanced Particle Microphysics, a state-of-the-art chemical transport model with a size-resolved particle microphysics model, generated air pollution simulation data. We then matched UFP exposure estimates to geocoded health records for asthma, bronchiectasis, chronic bronchitis, emphysema, unspecified bronchitis, and other chronic airway obstructions in New York State from 2013 through 2018. In addition, we assessed interactions with age, ethnicity, race, sex, meteorological factors, and season. Results: Each 1-(interquartile range [IQR]) increase in UFP exposure led to a 0.37% increased risk of a respiratory-related ED visit on lag 0-0, or the day of the ED visits, (95% confidence interval [CI], 0.23-0.52%) and a 1.81% increase on lag 0-6, or 6 days before the ED visit, (95% CI, 1.58-2.03%). The highest risk was in the emphysema subtype (lag 0-5, 4.18%; 95% CI, 0.16-8.37%), followed by asthma (lag 0-6, 2.00%), chronic bronchitis (lag 0-6, 1.78%), other chronic airway obstructions (lag 0-6, 1.60%), and unspecified bronchitis (lag 0-6, 1.49%). We also found significant interactions between UFP health impacts and season (Fall, 3.29%), temperature (<90th percentile, 2.27%), relative humidity (>90th percentile, 4.63%), age (children aged <18 yr, 3.19%), and sex (men, 2.06%) on lag 0-6. Conclusions: In this study, UFP exposure increased CLRD-related ED visits across all seasons and demographic characteristics, yet these associations varied according to various factors, which requires more research.

Association between neighborhood poverty and ovarian reserve: the ovarian aging study.

This study aimed to examine the association between neighborhood poverty and ovarian reserve. Among 1,019 healthy premenopausal women in the Ovarian Aging Study, aggregate exposure to neighborhood poverty was examined in relation to biomarkers of ovarian reserve, antimüllerian hormone (AMH) and antral follicle count (AFC). Specifically, the interaction of age-x-neighborhood poverty was assessed cross-sectionally to determine whether AMH and AFC declines across women may be greater in women exposed to more neighborhood poverty. Neighborhood poverty was assessed by geocoding and linking women's residential addresses in adulthood to US Census data. Independent of covariates, a significant interaction term showed the association between age and AMH varied by degree of exposure to neighborhood poverty in adulthood ( b = -0.001, P < 0.05). AMH declines increased progressively across women exposed to low, medium, and high levels of neighborhood poverty. In addition, main effects showed that higher neighborhood poverty was related to higher AMH in the younger women only ( b = 0.022, P < 0.01). Results related to AFC were all nonsignificant ( P > 0.05). Across women, greater aggregate exposure to neighborhood poverty in adulthood was related to lower ovarian reserve, indexed by AMH. In addition, there was a positive association between neighborhood poverty and AMH in younger women that attenuated in the older women. Together, results suggest that neighborhood disadvantage may have detrimental impacts that manifest as initially higher AMH, resulting in greater ovarian follicle loss over time. However, it remains unclear whether these results examining differences across women may replicate when AMH declines by neighborhood poverty are examined longitudinally.

Epicardial adipose tissue and pericardial constraint in heart failure with preserved ejection fraction.

Obesity and epicardial adiposity play a role in the pathophysiology of heart failure with preserved ejection fraction (HFpEF), and both are associated with increased filling pressures and reduced exercise capacity. The haemodynamic basis for these observations remains inaccurately defined. We hypothesize that an abundance of epicardial adipose tissue (EAT) within the pericardial sac is associated with haemodynamic signs of pericardial constraint. HFpEF patients who underwent invasive heart catheterization with simultaneous echocardiography were included. Right atrial pressure (RAP), right ventricular end-diastolic pressure, and pulmonary capillary wedge pressure (PCWP) were invasively measured. The presence of a square root sign on the right ventricular pressure waveform and the RAP/PCWP ratio (surrogate parameters for pericardial constraint) were investigated. EAT thickness alongside the right ventricle was measured on echocardiography. Sixty-four patients were studied, with a mean age of 73±10years, 64% women, and a mean body mass index (BMI) of 28.6±5.4kg/m2. In total, 47 patients (73%) had a square root sign. The presence of a square root sign was associated with higher BMI (29.3 vs. 26.7kg/m2, P=0.02), higher EAT (4.0 vs. 3.4mm, P=0.03), and higher RAP (9 vs. 6mmHg, P=0.04). Women had more EAT than men (4.1 vs. 3.5mm, P=0.04), despite a comparable BMI. Women with a square root sign had significantly higher EAT (4.3 vs. 3.3mm, P=0.02), a higher mean RAP (9 vs. 5mmHg, P=0.02), and a higher RAP/PCWP ratio (0.52 vs. 0.26, P=0.002). In men, such associations were not seen, although there was no significant interaction between men and women (P>0.05 for all analyses). Obesity and epicardial adiposity are associated with haemodynamic signs of pericardial constraint in patients with HFpEF. The pathophysiological and therapeutic implications of this finding need further study.

(095) Vaginal Lubrication Improves Sexual Desire among Menopausal Women

Abstract Introduction Female sexual dysfunction (FSD) can be a direct result of hormonal changes in a woman’s lifetime, including the use of birth control, postpartum, or menopausal. The FSFI (Female Sexual Function Index) measures sexual function based on six domains: desire, arousal, lubrication, orgasm, satisfaction and pain. Studies have shown that vaginal lubrication can be affected by hormonal changes; however, it is unclear how lubrication relates to sexual desire among women with hormonal changes of varied etiology. Objective The objective of this study is to evaluate the relationship between vaginal lubrication and sexual desire among women who are on birth control, postpartum, or menopausal. Methods A post-market analysis was performed of all women who had utilized the sexual wellness application Rosy between February 2022 and November 2022. Application users had previously filled out the FSFI short form (6-item) and the desire and lubrication domain results were analyzed. We utilized multivariable linear regression models to determine significant effects between lubrication score and desire score controlling for whether the patient was on birth control, postpartum, or menopausal. Results A total of 11,407 women met the study criteria. 2,599 were menopausal, 3,394 were postpartum, and 8,013 were on birth control. The median age was 34.2 (range 17–80) years old. The mean FSFI short form scores for lubrication were as follows based on category: birth control = 3.03, postpartum = 2.0, and menopausal = 1.94. Mean FSFI short scores for desire were birth control = 2.44, postpartum = 2.23, and menopausal = 1.80. Regression modeling revealed a significant association between lubrication score and desire score (mean of 0.36 increase in desire score per point increase in lubrication score, 95% CI 0.35, 0.36, p&amp;lt;0.001). With women on birth control as a baseline, a menopausal status was associated with an increased desire (estimate 0.33, p&amp;lt;0.001). The interaction term between lubrication score and menopausal status was positive (0.06, p&amp;lt;0.001). Conclusions Iatrogenic or physiologic hormonal changes such as birth control, postpartum, or menopause can alter vaginal lubrication scores. Among these women, those with better vaginal lubrication have an associated increase in sexual desire. Improvements in lubrication can positively affect sexual desire in menopausal women. Our findings of a significant positive interaction between lubrication scores and menopausal status is interpreted as the magnified effect that increase lubrication has on sexual desire in this population. Furthermore, these findings reinforce that among this cohort, menopausal women are most effected by hormonal changes that can alter lubrication and ultimately sexual desire. While this data demonstrates interesting findings, potential confounders include users of Rosy who may have underlying sexual dysfunction, which may alter these results. Further studies are needed on this topic among the general population. Disclosure Any of the authors act as a consultant, employee or shareholder of an industry for: Rosy.

Abstract B057: Oncogenic Foxl2 directs enhancer reprogramming and changes in 3D genome structure in ovarian granulosa cell tumors

Abstract Background: Adult-type granulosa cell tumors (aGCTs) are a rare monogenic disease. Nearly all aGCTs carry a missense point mutation in the Forkhead domain containing FOXL2 (Foxl2 p.C134W) transcription factor, but the oncogenic mechanism of this mutation is unknown. Other Forkhead family transcription factors have well-described “pioneer” activity, binding to compacted, nucleosome-bound DNA and increasing accessibility for other regulatory proteins. Objectives: To investigate if oncogenic Foxl2-C134W has pioneering activity and if mutant Foxl2 operates its oncogenic activity through enhancer reprogramming. Methods: CRISPR/Cas9 editing was used to generate isogenic granulosa cell tumor cells lacking either the FOXL2 wild-type allele (single knock-out; SKO) or both the mutant and wild-type FOXL2 alleles (double knock-out; DKO). ATAC-Seq and endogenous Foxl2 ChIP-Seq were performed on these isogenic lines to determine the differential chromatin accessibility at Foxl2-bound regulatory regions across genotypes. Promoter capture HiC was performed using a bespoke genome-wide hybrid capture probe set, CHiCAGO was used to identify significant HiC interactions, and an activity-by-contact model was constructed to identify enhancer elements bound by endogenous Foxl2-C134W and determine their target promoter contact frequency (promoter capture HiC) and enhancer activity (geometric mean of H3K27ac and ATACseq signal. Results: Endogenous ChIP-seq of Foxl2-C134W from the SKO cell line identified 1147 high-confidence peaks. De novo motif discovery identified the canonical Foxl2 binding motif (TGTTTACATT, P = 1.4 × 10−7) in 44.9% of peaks, as well as a novel variant motif (TGTTTTGTCT, P = 6.7 × 10−15) in 68.5% of peaks. Median chromatin accessibility at Foxl2-C134W peak regions, as measured by ATAC-seq, was significantly decreased in the DKO cells compared to either SKO or parental cell lines (P &amp;lt; 2 × 10-16 for both comparisons). Decreased chromatin accessibility at Foxl2-C134W ChIP-seq peaks in the DKO cells was driven by peaks containing the novel variant Foxl2 binding motif. Promoter capture HiC through activity-by-contact model identified promoter-enhancer interactions lost in the DKO cell line involving several genes, including ADAMTS5, FOXF2, and PRICKLE1 (adjusted P &amp;lt; 0.05). Conclusion: Foxl2-C134W exhibits “pioneering” activity, increasing chromatin accessibility at key gene regulatory elements with associated re-programming of promoter-enhancer interactions. The oncogenic mechanism of Foxl2-C134W in granulosa cell tumors may involve changes in DNA binding specificity, re-directing this pioneering function to sites containing a novel variant Foxl2 binding motif. Citation Format: Veena K. Vuttaradhi, Eleonora Khlebus, Thomas Welte, Barrett Lawson, Robert Tyler Hillman. Oncogenic Foxl2 directs enhancer reprogramming and changes in 3D genome structure in ovarian granulosa cell tumors [abstract]. In: Proceedings of the AACR Special Conference on Ovarian Cancer; 2023 Oct 5-7; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(5 Suppl_2):Abstract nr B057.

External cues improve visual working memory encoding in the presence of salient distractors in schizophrenia.

People with schizophrenia (PSZ) are impaired in attentional prioritization of non-salient but relevant stimuli over salient distractors during visual working memory (VWM) encoding. Conversely, guidance of top-down attention by external predictive cues is intact. Yet, it is unknown whether this preserved ability can help PSZ encode more information in the presence of salient distractors. We employed a visuospatial change-detection task using four Gabor patches with differing orientations in 66 PSZ and 74 healthy controls (HCS). Two Gabor patches flickered which were designated either as targets or distractors and either a predictive or a non-predictive cue was displayed to manipulate top-down attention, resulting in four conditions. We observed significant effects of group, salience and cue as well as significant interactions of salience by cue, group by salience and group by cue. Across all conditions, PSZ stored significantly less information in VWM than HCS. PSZ stored significantly less non-flickering than flickering information with a non-predictive cue. However, PSZ stored significantly more flickering and non-flickering information with a predictive cue. Our findings indicate that control of attentional selection is impaired in schizophrenia. We demonstrate that additional top-down information significantly improves performance in PSZ. The observed deficit in attentional control suggests a disturbance of GABAergic inhibition in early visual areas. Moreover, our findings are indicative of a mechanism for enhancing attentional control in PSZ, which could be utilized by pro-cognitive interventions. Thus, the current paradigm is suitable to reveal both preserved and compromised cognitive component processes in schizophrenia.

PERTUMBUHAN DAN HASIL CABAI RAWIT (CAPSICUM FRUTESCENS L.) PADA DOSIS TRICHODERMA, SP. DAN PUPUK ORGANIK PETROGANIK

PERTUMBUHAN DAN HASIL CABAI RAWIT (CAPSICUM FRUTESCENS L.) PADA DOSIS TRICHODERMA, SP. DAN PUPUK ORGANIK PETROGANIK

Associations between objective measures of performance-related characteristics and perceived stress in young cross-country skiers during pre-season training

ABSTRACT Monitoring performance-related characteristics of athletes can reveal changes that facilitate training adaptations. Here, we examine the relationships between submaximal running, maximal jump performance (CMJ), concentrations of blood lactate, sleep duration (SD) and latency (SL), and perceived stress (PSS) in junior cross-country skiers during pre-season training. These parameters were monitored in 15 male and 14 females (17 ± 1 years) for the 12-weeks prior to the competition season, and the data was analysed using linear and mixed-effect models. An increase in SD exerted a decrease in both PSS (B = -2.79, p ≤ 0.01) and blood lactate concentrations during submaximal running (B = -0.623, p ≤ 0.05). In addition, there was a negative relationship between SL and CMJ (B = -0.09, p = 0.08). Compared to males, females exhibited higher PSS scores and little or no change in performance-related tests. A significant interaction between time and sex was present in CMJ with males displaying an effect of time on CMJ performance. For all athletes, lower PSS appeared to be associated with longer overnight sleep. Since the females experienced higher levels of stress, monitoring of their PSS might be beneficial. These findings have implications for the preparation of young athletes’ competition season.

18 Progesterone and insulin concentrations during pregnancy in slick and wild type-haired Holstein cows

Abstract In cattle, the presence of the SLICK gene has been associated with shorter calving intervals when exposed to tropical climates. However, the effects this genotype may have in terms of circulating hormones during pregnancy have not been reported. Therefore, the current study aimed to determine if reproductive hormones concentrations differ during the gestation between slick (SH) and wild type-haired (WT) cattle. Holstein heifers were estrus synchronized and inseminated during Winter (December) and Summer (June). A total of 13 SH and 8 WT Holstein heifers became pregnant and are included in the current study. Beginning on the day of insemination (d 0), blood samples were collected every 28 d. Commercially available ELISA kits were used to determine the plasma concentrations of bovine progesterone and insulin. The effects of hair coat genotype, season, day of pregnancy, and the respective interactions were tested by PROC Mixed in SAS. Day of pregnancy was utilized as a repeated measure for each animal. A significant season x day of pregnancy interaction (P &amp;lt; 0.001) was observed for the plasma concentrations of progesterone. On gestational d 0, 28 and 56, progesterone concentrations were greater for heifers inseminated during the Winter (7.11 ± 0.50, 5.31 ± 0.51, 5.39 ± 0.49 ng/mL, for d 0, 28 and 56 of pregnancy, respectively) when compared with those inseminated during the Summer (3.04 ± 0.45, 3.17 ± 0.45, 3.21 ± 0.45 ng/mL, for d 0, 28 and 56 of pregnancy, respectively). For insulin, also a significant interaction of season x day of pregnancy was observed (P = 0.004). On d 112 of pregnancy, the concentrations of insulin were greater for heifers inseminated during Winter (0.337 ± 0.03 ng/mL) when compared with those inseminated during Summer (0.082 ± 0.08 ng/mL). No other significant interactions were observed. Neither differences in progesterone (P = 0.70; 3.7 ± 0.33 and 3.9 ± 0.25 ng/mL, for WT and SH, respectively) nor insulin (P = 0.46; 0.20 ± 0.02 and 0.18 ± 0.01 ng/mL, for WT and SH, respectively) were observed between genotypes. Further research comparing other hormones during pregnancy may provide useful information between genotypes that may help to explain the better reproductive performance of SH dairy cows.

132 Effects of supplementing corn silage to fall-calving cows grazing tall fescue on cow and calf performance and physiology

Abstract The objective was to test the effect of fescue varieties and supplemental feeding of cows grazing fescue pastures during the growing season on cow and calf performance and physiology. Multiparous and primiparous Angus crossbred cows (n= 45) averaging 151 ± 7 d of gestation at trial initiation, were stratified and assigned to pasture treatment based on estimated calving date and parity. Treatment design was a 2 x 2 factorial with two types of fescue pasture (toxin producing or non-toxin producing) and two levels of supplemental corn silage (non-supplemented or supplemented at 1% of BW DM basis). Each treatment replicate (pasture, n = 3 or 4 pastures per treatment) was stocked with 3 cows. There were seven replicates of non-toxin-producing fescue (5 ppm ± 2.50 SEM ergot alkaloids; 38.6% endophyte infection), and eight replicates of toxin producing K31 fescue pastures (507 ppm ± 0.82 SEM ergot alkaloids; 92.5% endophyte infection). Cows were fed corn silage (38% DM, 7.83% CP DM basis) daily in fenceline bunks and feeding amount was adjusted at weigh dates to maintain 1% of BW DM basis. Cows were weighed on two consecutive days at initiation and upon conclusion of the study after being fed similar diets for 4 d to minimize variation in ruminal fill. Body weight (BW), body condition score (BCS), hair score (HS), hair length (HL), and rump fat (RF) measurements were taken at initiation (the middle of the second trimester), trial mid-point (beginning of third trimester), and approximately 2 wk before estimated parturition. Cows remained on experimental pastures through parturition. Data were analyzed as a mixed model with pasture as experimental unit and cows within pasture as subsamples. Initial cow BW and BCS were 558 ± 11 kg (SEM), and 4.9 ± 0.08 (SEM), respectively. For all measures of interest there were no significant interactions observed (P &amp;gt; 0.16). Supplemental feeding tended (P = 0.07) to increase BW gain and rump fat (P = 0.05) regardless of fescue type. Cows grazing toxic fescue tended (P = 0.12) to have a longer hair coat. Likewise, cows grazing toxic fescue had less change in overall HS (P = 0.02). Supplemental feeding of corn silage to cows at 1% of BW DM basis while grazing fescue did not significantly affect gain differences or reduce visible symptoms typical of fescue toxicosis when grazing during the growing season.

Association of prognostic nutritional index with peripheral artery disease in US adults: a cross-sectional study

BackgroundThe objective of this study was to investigate the relationship between the prognostic nutritional index (PNI) and peripheral artery disease (PAD).MethodsThe present study is a cross-sectional study based on the National Health and Nutrition Survey (1999–2004). The laboratory-calculated PNI was divided into four groups based on quartiles(Q1:PNI ≤ 50.00; Q2: 50.01–53.00; Q3:53.01–56.00; Q4: > 56.00). PAD was defined as an ankle brachial pressure index (ABPI) ≤ 0.9 on the left or right. The relationship between PNI and PAD was examined using multifactor weighted logistic regression analysis, as well as subgroup analysis. Subgroup analyses were conducted based on demographic and clinical variables.ResultsA total of 5,447 individuals were included in our final analysis. The age of the participants was 59.56 ± 13.10 years, and males accounted for 52.8% (n = 2820). The prevalence of PAD was 6.7% (n = 363). After adjusting for all factors, participants with Q1 still had an increased risk of PAD, with an OR value of 1.593 and a 95% CI of 1.232–1.991. Subgroup analysis showed no significant interaction among multiple factors.ConclusionsIn summary, we report that lower PNI are associated with a higher risk of PAD in US adults. It is hoped that this discovery can provide a reference for the prevention of PAD.

User perception of animation fluency: The effect of time duration in different phases of animated transitions during application usage

Animation fluency is an important factor of user experience in smartphone applications. The purpose of this study is to explore the effect of time duration in different sections of application usage on users’ perceived fluency. We manipulated the duration of different phases, including the click-response delay (CD), duration of animation (DA), and duration of loading completion (DL), in an open app task and an in-app page switching task. The participants evaluated the perceived fluency during their smartphone use. The results showed that the CD and DL had a significant effect on perceived fluency and that the CD, DA and DL showed significant interactions in some situations. These results could supply detailed parameters for smartphone design and could be used to build a model to guide manufacturers to provide a better user experience.

Impact of sensory reweighting strategies on postural control using the sensory organization test in older adults with and without fall risks.

The Sensory Organization Test (SOT), an integral part of computerized dynamic posturography, plays a crucial role in evaluating postural stability under various altered sensory conditions. Despite its importance, there have been noted inconsistencies in the results pertaining to equilibrium and sensory system evaluations. This study aimed to compare four sensory analysis scores and equilibrium indices between older adults with and without fall risks. The study included 34 participants identified as being at risk of falls and 42 control subjects. To categorize individuals between the two groups, we performed area under the receiver operating characteristic curve analyses. This classification was based on scores from the Modified Falls Efficacy Scale (MFES) and the composite scores obtained from the SOT. In addition, we used the Tampa Scale for Kinesiophobia (TSK) as well as the level of disability. The fall risk group demonstrated significantly higher TSK scores (39.39± 15.24 for control group vs. 54.65±10.70 for fall risk group; t=-5.09, p=0.001). The groups demonstrated a significant interaction on the equilibrium index (F=4.59, p=0.03), which was lower in the fall risk group in Condition 6 with a moving surface and surround and eyes open (t=2.29, p=0.01). The fall risk group demonstrated a higher somatosensory score (t=-1.73, p=0.04). The fall risk group had a lower equilibrium index score in Condition six of the SOT, which was useful for identifying deficits in vestibular function to integrate sensory information under challenging conditions for postural adaptation. This strategy suggested that the fall risk group could compensate for their risk of falls by utilizing more effective somatosensory reweighting strategies compared with the control group.