Significant Differences In BW Research Articles

Establishment of health food safety evaluation method-In vivo genotoxicity evaluation by micronucleus assay

In recent years, various factors such as shifts in health consciousness, changes in lifestyle habits, increased focus on dietary intake, the promotion of alternative medical concepts, and advancements in life sciences and technology have propelled the robust growth of the health food industry. An increasing number of reports suggest that "functional foods," containing beneficial functional components, may enhance short-term well-being and are increasingly regarded as healthful. Building upon these findings, this study aims to enhance the production capacity of high-value agricultural products. However, for quality assurance, toxicity assessment of functional foods is imperative. There is a growing necessity to employ in vivo genotoxicity assays to evaluate the carcinogenic potential of these products. In this study, we tried to establish another in vivo genotoxicity evaluation platform via acridine orange induction. According to all results in this study, during the experiment, there were no statistically significant differences in BW between the negative control group and the normal control group (p > 0.05). Moreover, the clinical observations of the experimental mice showed that all mice survived until the end of the experiment, and no abnormal clinical symptoms were observed. The mouse’s food consumption in each group were monitored during the experiments. The food consumption of mice was recorded daily after the experiment. During the experiments, there were no significant difference between two groups on the food consumption. The percentage of RETs/1,000 RBCs in the negative control group was significantly decrease than the normal control group (p < 0.001). RETs/1,000 RBCs (‰) in ICR mice (n = 5/group) in the negative control group and the normal control group at 48th and 72th hours-experiment were respectively 18 ± 4.6 / 45.6 ± 4.0 (48th hours-experiment) and 19 ± 4.8 / 47.6 ± 4.8 (72th hours-experiment). The percentage of Mn-RETs/1,000 RETs in the negative control group was significantly increase than the normal control group (p < 0.001). Mn-RETs/1,000 RETs (‰) in ICR mice (n = 5/group) in the negative control group and the normal control group were 10.6 ± 2.1 / 0.4 ± 0.5 and 8.2 ± 1.8 / 0.4 ± 0.5, respectively. Taken all results of RET/RBCs (‰) and MN-RET/RETs (‰) together, the genotoxicity in the negative control group has been successfully induced. In light of these requirements, we propose utilizing the mammalian erythrocyte micronucleus assay to detect damage induced by test substances to erythroblast chromosomes. This study endeavors to establish a sensitive and stable method for quantifying micronuclei formation in erythrocytes in vivo. The resulting data will facilitate the evaluation of potential toxicity associated with agricultural functional products in the future.

The Effect of including a Mixed-Enzyme Product in Broiler Diets on Performance, Metabolizable Energy, Phosphorus and Calcium Retention.

The importance of enzymes in the poultry industry is ever increasing because they help to extract as many nutrients as possible from the raw material available and reduce environmental impacts. Therefore, an experiment was conducted to examine the effect of a natural enzyme complex (ASC) on diets low in AME, Ca and P. Male Ross 308 broilers (n = 900) were fed one of four diets: (1) positive control (PC) with no enzyme added (AME 12.55 MJ/kg, AVPhos 4.8 g/kg and AVCal 9.6 g/kg); (2) negative control (NC) with no enzyme added and reduced AME, Ca and P (AME 12.18 MJ/kg, AVPhos 3.3 g/kg, AVCal 8.1 g/kg); (3) negative control plus ASC at 200 g/t; and (4) negative control plus ASC at 400 g/t. Broiler performance, digesta viscosity, tibia mineralization and mineral content were analyzed at d 21. Between d 18 and 20, excreted DM, GE, total nitrogen, Ca, and P were analyzed. ASC at 200 g/t and 400 g/t improved the FCR (p = 0.0014) significantly when compared with that of the NC. There were no significant differences in BW or FI between the treatments. Birds fed ASC at 200 g/t and 400 g/t had significantly improved digesta viscosity (p < 0.0001) compared with that of the PC and NC birds and had significantly higher excreted DM digestibility (p < 0.01) than the NC and the PC birds with 400 g/t ASC. ASC inclusion significantly improved P retention (p < 0.0001) compared to that in the PC. Ca retention was significantly increased by 400 g/t ASC compared to that in the PC and NC (p < 0.001). AME was significantly higher (p < 0.0001) for all treatments compared to that in the NC. There were no significant differences between treatments for any of the bone measurements. This study showed that feeding with ASC can support the performance of broilers when fed a diet formulated to have reduced Ca, P and AME, with the greatest results being seen with a higher level of ASC inclusion.

Performance and processing yield comparisons of Large White male turkeys by genetic lines, sources, and seasonal rearing

Large White male turkey genetic lines (GL) comparison in performance and processing yields under the same conditions are rare in the literature. Two rearing experiments (EXP) were conducted to accomplish 2 objectives. The first objective was to test the effects of poult source and genetic lines on performance and processing yields. The second objective was to extract season and growth patterns when comparing both EXP common treatments. In EXP 1, male poults from 5 different sources were randomly assigned to 48 concrete: litter-covered floor pens. In EXP 2, male poults from 7 different genetic lines were randomly assigned to 48 concrete: litter-covered floor pens. For both EXP, the experimental design was a completely randomized block design with a one-factor arrangement. Both EXP were placed in the same house with the same management and nutrition in two separate seasons of the same year. Bird performance and carcass processing yield were analyzed in SAS 9.4 or JMP 15.1 in a mixed model. In EXP 1 no significant difference in BW or processing yield was observed. However, a similar GL from a commercial hatchery had an improved feed conversion ratio (FCR) over the same GL sourced directly from the genetic company hatchery. In EXP 2, statistical differences were observed in performance and breast meat yield depending on the GL. A season effect was observed when comparing the two EXP. Birds raised in the fall season had a 2 kg BW increase, on average, over their spring counterparts. This difference in BW can also be observed in a statistically higher breast meat yield by the birds raised in the fall over the ones raised in the spring. In conclusion, a comparison between GL resulted in effects due to genetic line, poult source, and rearing season on bird performance and carcass yield.

The effects of supplemental levels of inorganic zinc, manganese and copper on growth performance in commercial broilers

Commercial broiler premixes provide trace minerals (TMs) such as Zinc (Zn), Manganese (Mn) and Copper (Cu) above the birds' requirements to maximise broiler performance. High inclusion levels of TM and their low absorption in the broiler's gastrointestinal tract have led to increased TM levels in their litter, raising concerns about TM's accumulation in the environment from poultry litter. A 35-day broiler trial was conducted at a broiler facility with 2 880-day-old Cobb 500 broiler males. The objective was to evaluate whether decreasing inorganic Zn, Mn and Cu levels in broiler diets would have a negative effect on broiler growth parameters such as BW, average daily gain, cumulative feed intake (FI) and feed conversion ratio (FCR). Consequently, these minerals were supplemented at 100%, 50%, 25%, and 0% of the Cobb standards. No significant difference in FI and FCR was observed between the treatments for the first 21 days, and there were no significant differences in BW between the positive control, negative control (NC), and 50% of Cobb recommended levels at day 35. However, BW for the NC was significantly higher than the 25% level. These results show that reducing TM levels in broiler diets below commercially recommended levels had no negative effect on broiler performance, with the subsequent economic benefits for producers.

Nutrient profile and effects of carinata meal as alternative feed ingredient on broiler performance, tight junction gene expression and intestinal morphology

Two studies were conducted to establish carinata meal as a partial replacement of conventional protein sources. Study I was conducted to determine the nutrient profile, nitrogen-corrected true metabolizable energy (TMEn), and amino acid (AA) digestibility of 2 groups: low glucosinolate carinata meal (LGCM) and high glucosinolate carinata meal (HGCM) using rooster assays. The LGCM contained 28 μmol/g glucosinolate, 11.5% moisture, 39.2% crude protein (CP), whereas the HGCM had 100 μmol/g glucosinolate, 10.1% moisture, 39.5% CP on as is basis. The precision-fed rooster assays were conducted to determine TMEn and AA digestibility. The TMEn levels of LGCM and HGCM were 1,814 and 1,690 kcal/kg on as is basis, respectively. Standardized digestibility for lysine, methionine, cysteine, threonine, and valine were 72, 88, 69, 75, and 79% for LGCM and 80, 89, 71, 76, and 80% for HGCM, respectively. Based on the nutrient profiles from study I, study II was conducted to evaluate the effects of LGCM and HGCM in broilers. A total of 504 one-day-old Cobb500 male broiler chickens were randomly divided into 42 battery cages with 6 replicates of 12 birds per cage. The seven dietary treatments were control diet, 3 inclusion levels of LGCM (4, 8, and 12%), and 3 of HGCM (4, 8, and12%) in a corn-SBM based diet fed for 21 d. No significant differences in BW, BWG, and FI were observed except for significantly lower BWG in 12% HGCM group compared to control for 14-21 days (P < 0.05). The FCR for 12% HGCM increased significantly compared to 4 and 8% of both LGCM and HGCM groups during wk 3 (14–21 d). Based on these studies, carinata meal could be recommended to partially replace conventional feed ingredients at a rate of 12% when LGCM is used and 8% when HGCM is used with no deleterious effects on growth performance, gut histology, and tight junction proteins.

Analysis of chemical compounds and toxicological evaluation of Forsythia suspensa leaves tea.

To determine the compositions of Forsythia suspensa leaves tea (FSLT) and its safety, the chemical compounds were analysed with some methods, and the toxicity was evaluated in Kunming mice and Wistar rats. The results showed that FSLT contained rich flavonoid, lignans, triperpene acids, amino acids, and mineral elements. In the acute toxicity study, none of the mice died, and no obvious poisoning symptoms were observed after 14days in mice at the dose of 15mg/g·body weight (bw) FSLT; in the sub-chronic toxicity, no abnormal or dead rat was found at the dose of 1, 3, and 10mg/g·bw during 90days feeding administration; there was no significant difference in bw and food consumption; no significant differences were found in each hematology and serum biochemistry parameter and organ/body weight ratio comparing with the control experimental group. The results revealed that the FSLT has low or no toxicity via oral administration. Therefore, FSLT is very suitable and safe to be used as a new resource food.

Morphological Investigations of the Effect of Thermal Manipulation During Embryogenesis on Body Performance and Structure of Pectoral and Thigh Muscle of Ross Broiler Chicken

ABSTRACT The aim of this study was to determine the optimum timing of thermal manipulation during early, mid, late and long lasting embryogenesis that may result in improvement of body performance and myofiber development (fiber diameter and thickness of perimysium) of pectoral and thigh muscles by a comparative morphometrical investigation on post-hatch day 35. 1440 fertile Ross broiler eggs were divided randomly and equally into 5 treatment groups: control (no TM), TM1, TM2 TM3 and TM4 were thermally subjected to 39oC for 18h with 65% relative humidity daily during embryonic days ED 7-11, ED 11-15, ED 15-18 and ED 7-18 respectively. Out of TM conditions that were investigated, TM1 resulted in a significant improvement of hatchability rate (95.14%)and body performance when compared to the control. On post-hatch day 35, there were significant differences in BW, skinned carcass, breast, thigh and gut weight for all groups when compared to each other with a higher significant in TM1 and TM2 when compared to those of the control. FCR in this study was lower in TM groups when compared to the control group. In TM3 and TM4 the chicks show a significantly lower value of body performance when compared with those of the control group, TM1 and TM2.The histological evaluation of Pectoral muscle revealed myofibers of different diameter (38.39-40.27 µm) and perimysial thickening (17-30.5 µm), while myofibers diameter of thigh muscle ranged between (29-32.9 µm) and the perimysial thickening ranged between (14.36-22.15 µm). Thus, these results indicate that TM duringED7-11 improved muscle growth and body performance and this finding may be applied by commercial breeders to produce more enhanced broiler chickens.

The co-injection of antioxidants with foot-and-mouth disease vaccination altered growth performance and blood parameters of finishing Holstein steers.

ObjectiveThis study was conducted to evaluate whether the co-injection of antioxidants together with foot-and-mouth disease (FMD) vaccination has the potential to attenuate the negative effects caused by vaccination in Holstein finishing steers.MethodsA total of 36 finishing Holstein steers (body weight [BW]: 608±45.6 kg, 17 months old) were randomly allocated to one of three treatments: i) control (CON, only FMD vaccination without any co-injection), ii) co-injection of commercial non-steroidal anti-inflammatory drugs (NSAID) with FMD vaccination at a ratio of 10:1 (NSAID vol/FMD vaccine vol) as a positive control (PCON), iii) co-injection of commercial mixture of vitamin E and selenium with FMD vaccination (VITESEL) (1 mL of FMD vaccine+1 mL of antioxidants per 90 kg of BW). Changes in growth performance and blood parameters because of treatments were determined.ResultsNo significant difference in BW, average daily gain, and dry matter intake of the steers was observed among the treatments. The FMD vaccination significantly increased white blood cells (WBC), neutrophils, platelets, and mean platelet volume (p<0.01) in blood analysis. The count of lymphocyte tended to increase after vaccination (p = 0.08). In blood analysis, steers in VITESEL tended to have higher numbers of WBC, neutrophils, and platelets compared to that of other treatments (p = 0.09, 0.06, and 0.09, respectively). Eosinophils in VITESEL were higher than those in PCON (p<0.01). Among blood metabolites, blood urea nitrogen and aspartate transaminase were significantly increased, but cholesterol, alanine transferase, inorganic phosphorus, Mg, and albumin were decreased after FMD vaccination (p<0.01).ConclusionThe use of antioxidants in FMD vaccination did not attenuate growth disturbance because of FMD vaccination. The metabolic changes induced by vaccination were not controlled by the administration of antioxidants. The protective function of antioxidants was effective mainly on the cell counts of leukocytes.

Effects of Diet on Bile Acid Metabolism and Insulin Resistance in Type 2 Diabetic Rats after Roux-en-Y Gastric Bypass.

Roux-en-Y gastric bypass (RYGB) is effective for the treatment of type 2 diabetes mellitus; however, the mechanism remains unclear. The effects of RYGB on postprandial responses to three different diets (low carbohydrate (CH)-rich diet, high CH-rich diet, and fat-rich diet) of different nutritional composition in a Goto-Kakizaki (GK) diabetic rat model were assessed by measuring glucose tolerance, insulin resistance, incretin responses, and bile acid (BA) metabolism. GK-RYGB group rats lost weight and preferred low CH-rich diet, but there were no significant differences in BW among the different diets. Glucose tolerance and insulin resistance were improved in rats who underwent RYGB, together with higher levels of circulating BAs, plasma GLP-1, and PYY levels. GK-RYGB rats fed high CH-rich or fat-rich diet showed increased glucose level and insulin resistance, together with high plasma BA, GIP, and PYY levels compared to those fed a low CH-rich diet. RYGB improves glucose tolerance and insulin resistance which may be related to BA metabolism and hormone levels, and the nutrient composition of the diet affects the treatment effect of RYGB on T2DM.

Effect of different sources and levels of iron in the diet of sows on iron status in neonatal pigs.

This study was conducted to determine the effects of maternal dietary supplementation of ferrous glycine chelate (Fe-Gly) and ferrous sulfate monohydrate (FeSO4·H2O) on the relative organ weight, tissue iron contents, red blood cells (RBC), hemoglobin concentration (HGB) and hematocrit (HCT) in blood, as well as ferritin (Fn), serum iron (SI), and total iron binding capacity (TIBC) in serum of newborn piglets. Forty-five sows (Landrace × Large white, mean parity 3 to 4, no significant differences in BW) were randomly allotted to 9 treatments (n = 5 sows/treatment): control (basal diet with no Fe supplementation), the basal diet supplemented with 50, 80, 110 or 140 mg Fe/kg as Fe-Gly, and the basal diet supplemented with 50, 80, 110 or 140 mg Fe/kg as FeSO4·H2O. The neonatal piglets (n = 45) were used to determine the relative organ weight, tissue iron contents and blood biochemical indices. Compared with the control, the relative weight of spleen and kidney were significantly increased (P < 0.05) in the Fe-Gly groups. The iron contents in liver, spleen, kidney and femur were also found increased (P < 0.05) in the Fe-Gly groups. The RBC (d 1 and 21), HGB (d 1 and 21) and HCT (d 1 and 21) in blood and Fn (d 1) and SI (d 1 and 21) significantly increased (P < 0.05), but the TIBC (d 1 and 21) in serum decreased (P < 0.05) in the Fe-Gly groups. Moreover, the kidney relative weight, iron content in liver, spleen, kidney and femur, RBC (d 1) and HGB (d 21) in blood, and SI (d 1) in the Fe-Gly groups increased (P < 0.05) compared with the FeSO4·H2O treatment. Linear and quadratic responses of the kidney relative weight, the iron content in liver, spleen, kidney and femur, RBC (d 1 and 21), HGB (d 1 and 21) and HCT (d 1 and 21) in whole blood, SI (d 1) and TIBC (d 1 and 21) in the Fe-Gly groups were observed (P < 0.05). Linear responses of Fn (d 1 and 21) and SI (d 21) in the Fe-Gly groups, and spleen relative weight, HCT (d 1), Fn (d 1) and TIBC (d 1 and 21) in the FeSO4·H2O groups were observed (P < 0.05). These finding suggest that Fe-Gly supplemented at the level of 110 mg/kg in the diet of sows in this experiment is superior to other forms of supplementation, based on HGB concentration, the relative organ weight, tissue iron contents and blood biochemical indices of piglets.

449: Discrepant Doppler values in paired umbilical arteries in the growth restricted fetus and the relationship to fetal biometry

Managing fetal growth restriction (FGR) with umbilical artery (UA) Doppler improves outcomes. Recommendations are to sample the UA in a free loop of the mid-segment, but no guidelines exist for sampling both (paired) UA in FGR. We previously reported discrepant paired UA in a FGR cohort, but a paucity of data to support the approach makes clinical significance unclear. We hypothesized that patients with discrepant paired UAs would have biometric trends similar to those with both UAs abnormal. In this prospective observational study, 73 patients with estimated fetal weights (EFW) <10th%, were followed serially between 24 and 38 weeks gestational age (GA). Doppler and standard biometry including femur length (FL), abdominal circumference (AC), head circumference (HC), and biparietal diameter (BPD) were obtained. After delivery, patients were assigned to 1 of 3 groups: Group 1: both UA normal at last exam (n=49); Group 2: multiple exams with discrepancies (n=13); Group 3: both UAs abnormal at last exam (n=8). Biometry values were plotted over GA. Differences in slope or average difference in y-values between groups were analyzed with a linear mixed model ANOVA in SAS. Available outcome data: birth weight percentile (BW%), GA at delivery, and Ponderal index (PI) were compared across UA groups with one-way ANOVA. There is a linear and parallel increase of all biometric measurements across GA. Table 1 shows biometric values among groups. AC and FL are smaller in groups 2 and 3 compared to group 1, but no difference exists between groups 2 and 3. EFW is lower in group 3, compared to group 1. HC is smaller in group 3 compared to groups 1 and 2. BPD is similar between groups. BW% is lower in group 3 compared to group 1 and PI is trending towards significance (Fig 1). The similar and significant decrease in FL and AC between discrepant (group 2) and abnormal UA Doppler (group 3) groups suggests that an abnormality in at least one UA may be an effective marker of FGR placental pathology. HC and BPD did not demonstrate similar findings, which is not surprising given brain sparing associated with FGR, and likely explains the lack of EFW significance. While outcome showed significant differences in BW%, additional data are needed to improve the power of the study. Our results suggest pathology with one abnormal UA. (Funded by The Perelman IUGR Study).View Large Image Figure ViewerDownload Hi-res image Download (PPT)

Abstract P188: (Pro)renin Receptor (PRR) Mediates High Fat Diet-induced Hypertension via Upregulation of Epithelial Sodium Channel &amp; Aquaporin-2

We hypothesized that the renal prorenin receptor (PRR) mediates high fat diet (HFD)-induced hypertension via enhancing expression of epithelial sodium channel (α-ENaC) and aquaporin-2 (AQP-2). C57BL/6 mice underwent right nephrectomy (n=12) and allocated to receive regular diet (RD, 12 kcal% fat, n=6) or a high-fat diet (HFD, 45 kcal% fat, n=6) for 10 weeks. HFD group received left renal subcapsular interstitial administration of PRR shRNA (n=3) or vehicle (n=3). BW, food and water intake, BP, UV and UNaV, renal interstitial fluid (RIF) angiotensin II (Ang II), and renal expressions of PRR, α-ENaC, AQP-2 were monitored. At baseline, there were no significant differences in BW, food and water intake, BP, UV or UNaV between different animal groups. At the end of the study, HFD mice had significant increase in food intake, systolic blood pressure (HFD 154.6 ± 2.181 mmHg, vs. RD 112.21 ± 4.684 mmHg, P&lt;0.0.05), BW (HFD 43.2 ± 1.125 gm, vs. RD 31.9 ± 0.89654 gm, P&lt;0.0.05), and significant reduction in UV (HFD 0.16 ± 0.042 ml, vs. RD 0.41 ± 0.061 ml, P&lt;0.0.05) and UNaV (HFD 26.02 ± 3.652 μmol/day vs. RD 46.32 ± 5.236 μmol/day, P&lt;0.0.05). Compared to RD, there were significant increases in mRNA and protein expressions of PRR (64% and 40%, P&lt;0.01), α-ENaC (85% and 75%, P&lt;0.05), AQP-2 (105% and 80%, P&lt;0.05) in HFD alone mice respectively. Compared to HFD alone, HFD+ PRR shRNA treatment caused significant reductions in BP (108 ± 13.88 mmHg vs HFD 154.6 ± 2.181 mmHg), mRNA and protein expressions of PRR (33% and 50%, P&lt;0.01), α-ENaC (49% and 56%, P&lt;0.05), AQP-2 (30% and 29%, P&lt;0.05) respectively. There were no changes in RIF Ang II between different animal groups. We conclude that PRR mediates HFD-induced hypertension via enhancing renal α-ENaC and AQP-2 expression independent of Angiotensin II.

The analysis of meat traits of Sussex cockerels and capons (S11) at different ages

The aim of the study was to compare Sussex cockerels and capons as well as to estimate the influence of age on slaughter yield and meat quality. The tests were performed on cockerels and capons from strain S11 (Sussex). At 16, 18, and 20 wk of age, a dissection of the entire carcass was conducted. The breast and leg muscles were tested for physio-chemical traits, as well as chemical parameters. It was noted that, due to significant differences in BW of the eviscerated carcasses between wk 18 and 20 of slaughter, the best time to cease rearing S11 cockerels could be wk 20. However, for S11 capons, the optimal time for slaughter appeared to be wk 18 (no significant differences in BW and carcass weight or musculature between wk 18 and 20). The trait which did not differ between cockerels and capons slaughtered at different ages was slaughter yield. Fatness of the cockerels increased with age whilst the weight of capons’ skin with subcutaneous fat was the same at wk 18 and 20 of evaluation. With increasing age, pH indicators of cockerels and capons’ breast muscles were increasing; the water holding capacity of capons’ breast muscles were also increasing. Both cockerels and capons showed darker color of breast muscle at wk 20 compared to wk 16 of evaluation. Redness of the breast muscle in the following periods of evaluation did not show significant differences, although with age, the yellowness of the cockerels and capons’ breast muscle increased significantly. Among cockerels and capons, the water content in the breast and leg muscles were decreasing with age whilst the protein content was increasing. A significant growing tendency of the percentage of fat share in the breast and leg muscles with age was noted in the capon group. Conclusions for breeding practice are as follows: due to meat and quality traits, Sussex cockerels and capons can be used until wk 18 or 20 of life.

Using near infrared transmittance to generate sorted fractions of Fusarium-infected wheat and the impact on broiler performance

The objective of this study was to investigate the effects of naturally contaminated Fusarium wheat containing deoxynivalenol (DON) on growth and performance of broiler chickens from 0 to 35 d. The BoMill TriQ individual kernel sorting technology uses near infrared transmittance (NIT) spectra to separate Fusarium-damaged kernels (FDK) from healthy kernels based on individual kernel CP. Three Fusarium-contaminated wheat sources were individually sorted into 3 test fractions: outlier (10% of the source), high mycotoxin (20% of the source), and low mycotoxin (70% of the source). These fractions were reconstituted into 4 ratios—M0, M20, M40, and M60—relating to the proportion of the high mycotoxin fraction in the reconstituted diets. These 12 reconstituted wheat sources with varying levels of DON were incorporated at ∼70% (starter) or ∼75% (grower/finisher) into diets. The fractions of wheat used had FDK ranging from 0.1 to 25.8% and DON from 0.0 to 14.3 ppm. A total of 480 newly hatched Ross 308 male broilers were randomly divided into 96 cages. Each test diet was assigned to 8 replicates with 5 birds per replicate cage. At 21 d, 180 birds were transferred to 36 cages, allowing 3 replicates of 5 birds per diet until 35 d. A factorial arrangement analysis compared the 3 wheat sources and 4 ratios produced from each sorted wheat. Growth and performance were evaluated as BW (g), feed intake (FI; g/bird/day), feed conversion ratio (FCR; g:g), AME (kcal ME/kg diet), nitrogen retention (NR; %), and mortality (%) for 0 to 21 d and 21 to 35 d. Results indicate no significant difference in BW, FI, and FCR (P > 0.05). Significant differences were seen in AME and NR (P < 0.01). This study demonstrates the potential of this novel sorting technology to produce naturally contaminated diets with a large range of mycotoxin concentrations from a single wheat source to enable future investigations of mycotoxin exposure in any species.

Performance and carcass characteristics of Delaware chickens in comparison with broilers

The recent resurgence in the popularity of keeping small flocks of chickens has brought with it an interest in the production characteristics of heritage breeds of chicken. The Delaware breed was developed in the state of Delaware for meat production and is considered a heritage breed because it has long been passed over in favor of the Cornish cross. The definition of a heritage chicken breed is one that physically conforms to the standards of the American Poultry Association, mates naturally, has a long, productive outdoor lifespan, and a slow growth rate. Production information on raising meat Delawares is lacking, as recent research has focused on diet formulation and genetics for broiler production. This study was undertaken to determine the production efficiency of Delawares as compared with broilers in BW, feed intake, FCR, and carcass dressing percentage. Significant differences in BW were noted between broilers and Delawares throughout the study; this was evident even with chick weights. Delawares took 15 wk to reach the same live BW as that of a broiler at 6 wk. The feed intake was greater for the broilers over Delawares during their 6-wk grow-out. The Delawares required twice as much feed to reach the same BW as that of the broiler. Significant differences between the 2 breeds were observed with regard to FCR, the broilers being the more efficient breed. The Delawares had a significantly lower carcass dressing percentage, just under 65%, as compared with broilers, just over 68%. It is hoped that this information will aid producers of niche-market heritage chickens in their decision-making when considering this breed for meat production.

Effect of corn silage and quantitative feed restriction on growth performance, body measurements, and carcass tissue composition in White Kołuda W31 geese

The objective of this study was to determine the effect of corn silage and quantitative feed restriction on BW, ADG, feed conversion, and carcass composition of White Kołuda W31 geese. Two diets were fed during the rearing period from 22 to 98 d of age: 1) a commercial diet ad libitum, and 2) restricted amounts of a commercial diet and corn silage ad libitum. Each treatment had 2 replicates of 16 birds each. From 99 to 119 d of age, all birds were fattened with whole oat grain alone. Incorporation of corn silage reduced weight gains and caused statistically significant differences in BW at the end of the rearing period (14 wk, 6,625.0 vs. 6,050.0 g;P < 0.05). Experimental geese showed compensatory growth during the oat fattening period and the BW of geese from both groups was similar at the end of the study (17 wk, 7,675.1 vs. 7,467.9 g;P > 0.05). Daily weight gains varied with week of growth, being lowest at 12 wk of age. Birds fed the commercial diet and corn silage had a significantly longer trunk (29.2 vs. 31.0 cm,P < 0.05) and shorter shanks (10.0 vs. 9.4 cm,P < 0.05) at 8 wk, and significantly smaller chest circumference (54.7 vs. 51.9 cm,P < 0.05) at the end of 14 wk. At the end of oat feeding (17 wk), geese receiving silage had significantly longer trunk and drumstick compared with geese fed commercial diets alone. The carcasses of 17-wk-old experimental geese contained more breast and leg muscles (%), and less skin with subcutaneous fat from breast and legs compared with control birds. Significant differences were only noted between the groups in dressing percentage (65.0 vs. 74.7%,P < 0.05) and proportion of skin with subcutaneous fat from breast (8.9 vs. 7.8%,P < 0.05). Dilution of the diet for young fattening geese with whole-crop corn silage had a positive effect on production economics and carcass composition.

The effects of yeast feed supplementation on turkey performance and pathogen colonization in a transport stress/Escherichia coli challenge

A commercial yeast culture feed supplement (YC; Celmanax SCP, Vi-COR, Mason City, IA) was provided to turkeys throughout a 16-wk grow-out to determine if it would prevent the effects of stress on production and pathogen colonization. The YC was provided either continuously at 100 g/t (YC-CS) or intermittently during times of stress at 200 g/t (YC-IS). Birds were stressed with an environmental challenge of Escherichia coli and by transporting them in a vehicle for 3 h after which they were penned in new social groups, without feed or water, for an additional 9 h. Turkeys were transported and challenged at 6, 12, and 16 wk of age to model the movement of birds within a 3-stage housing system. The YC-IS was provided only for the first week after hatch and for a 1-wk period encompassing each challenge. At wk 7 and 9, a decrease in BW of challenged birds was prevented by YC-IS but not YC-CS. There were no significant differences in BW due to either challenge or YC during wk 11 and 13. At wk 16, the challenge decreased BW, but there was no improvement in either of the YC treatments. Overall feed conversion ratio (FCR) was increased by transport/E. coli (P < 0.0001). The YC-CS improved FCR of challenged birds by 21 points, whereas YC-IS improved FCR by 36 points and this effect was significant (P = 0.013). The YC-CS tended to decrease both Salmonella and Campylobacter isolation from the ceca of stressed birds (P > 0.05). The YC-IS also tended to decrease Salmonella isolation (P > 0.05) with no effect on Campylobacter isolation. These data suggest that the practice of transporting turkeys decreases performance and that YC-IS may be more effective than YC-CS for alleviating the effects of this stressor on feed efficiency.

Efficacy and Safety of a Chinese Herbal Medicine Formula (RCM-104) in the Management of Simple Obesity: A Randomized, Placebo-Controlled Clinical Trial

Objective. This study was to evaluate the efficacy and safety of a Chinese herbal medicine formula (RCM-104) for the management of simple obesity. Method. Obese subjects aged between 18 and 60 years were selected for 12-week, double-blind, randomized, placebo-controlled trial. Subjects were randomly assigned to take 4 capsules of either the RCM-104 formula (n = 59) or placebo (n = 58), 3 times daily for 12 weeks. Measures of BW, BMI and WC, HC, WHR and BF composition were assessed at baseline and once every four weeks during the 12 week treatment period. Results. Of the 117 subjects randomised, 92 were included in the ITT analysis. The weight, BMI and BF in RCM-104 group were reduced by 1.5 kg, 0.6 kg/m2 and 0.9% and those in the placebo group were increased by 0.5 kg, 0.2 kg/m2 and 0.1% respectively. There were significant differences in BW and BMI (P < 0.05) between the two groups. Eleven items of the WLQOQ were significantly improved in the RCM-104 group while only 2 items were significantly improved in the placebo group. Adverse events were minor in both groups. Conclusion. RCM-104 treatment appears to be well tolerated and beneficial in reducing BW and BMI in obese subjects.

Fusarium mycotoxin-contaminated wheat containing deoxynivalenol alters the gene expression in the liver and the jejunum of broilers

The effects of mycotoxins in the production of animal feed were investigated using broiler chickens. For the feeding trial, naturally Fusarium mycotoxin-contaminated wheat was used, which mainly contained deoxynivalenol (DON). The main effects of DON are reduction of the feed intake and reduced weight gain of broilers. At the molecular level, DON binds to the 60 S ribosomal subunit and subsequently inhibits protein synthesis at the translational level. However, little is known about other effects of DON, for example, at the transcriptional level. Therefore, a microarray analysis was performed, which allows the investigation of thousands of transcripts in one experiment. In the experiment, 20 broilers were separated into four groups of five broilers each at day 1 after hatching. The diets consisted of a control diet and three diets with calculated, moderate concentrations of 1.0, 2.5 and 5.0 mg DON/kg feed, which was attained by exchanging uncontaminated wheat with naturally mycotoxin-contaminated wheat up to the intended DON concentration. The broilers were held at standard conditions for 23 days. Three microarrays were used per group to determine the significant alterations of the gene expression in the liver (P < 0.05), and qPCR was performed on the liver and the jejunum to verify the results. No significant difference in BW, feed intake or feed conversion rate was observed. The nutrient uptake into the hepatic and jejunal cells seemed to be influenced by genes: SLC2A5 (fc: −1.54, DON2.5), which facilitates glucose and fructose transport and SLC7A10 (fc: +1.49, DON5), a transporter of d-serine and other neutral amino acids. In the jejunum, the palmitate transport might be altered by SLC27A4 (fc: −1.87, DON5) and monocarboxylates uptake by SLC16A1 (fc: −1.47, DON5). The alterations of the SLC gene expression may explain the reduced weight gain of broilers chronically exposed to DON-contaminated wheat. The decreased expressions of EIF2AK3 (fc: −1.29, DON2.5/5) and DNAJC3 (fc: −1.44, DON2.5) seem to be related to the translation inhibition. The binding of DON to the 60 S ribosomal subunit and the subsequent translation inhibition might be counterbalanced by the downregulation of EIF2AK3 and DNAJC3. The genes PARP1, MPG, EME1, XPAC, RIF1 and CHAF1B are mainly related to single-strand DNA modifications and showed an increased expression in the group with 5 mg DON/kg feed. The results indicate that significantly altered gene expression was already occurring at 2.5 mg DON/kg feed.

Influence of red light on reproductive performance, eggshell ultrastructure, and eye morphology in Thai-native hens

In total, 120 Thai-native pullets (Gallus domesticus) aged 18 wk were housed in floor pens, located in a conventional open-sided shed under natural daylight (12L:12D) and randomly divided into 3 groups; Groups 1 (DF) and 2 (DR) were reared under natural daylight and supplemented with fluorescent or red light, respectively, whereas group 3 (R) was maintained in light-controlled pens and exposed only to red light. The red light was produced by light-emitting diodes. All treatments were provided with 16 h of light per day (16L:8D) during a 26-wk egg-laying period, and there were 4 replicate pens of 10 hens for each treatment. Photostimulation of these light sources was initiated at 18 wk of age and subsequent effects on reproductive performance were observed during the experimental period. Morphological characteristics of the eyes and eggshell microstructure were examined at the end of the study. Feed and water were provided ad libitum. There were no significant differences in BW, feed intake, egg weight, egg quality, or mortality rate due to the treatment. Pullets in the R and DR treatment groups commenced egg production significantly earlier than those in the DF treatment group. In early-season egg production (0-8 wk), cumulative egg number was significantly (P < 0.05) higher for the R treatment (25.9 eggs/hen) than for the DR (20.9) and DF (19.5) treatment groups. No significant differences in total egg production per hen occurred among the treatment groups. At 2 wk following photostimulation, hens in the R treatment group had significantly (P < 0.05) higher serum estradiol concentrations compared with hens in the other treatment groups. Neither eggshell structure nor eye morphology was affected by the treatments. It was concluded that the spectrum of red light did not affect live performance, egg production, egg quality, eggshell microstructure, or eye morphology of Thai-native hens, except for in accelerating sexual development. The light-emitting diode lighting system would be beneficial for energy savings and the reduction of rearing costs.