Significant Depletions Research Articles

P1319 Multi-omic modelling of antibiotic perturbed faecal microbial communities demonstrates reduced microbial resilience in IBD

Abstract Background Excessive antibiotic use is a risk factor in IBD, due to the impact on the gastrointestinal microbiota, but the extent of impact on microbial metabolite production is unknown. Here we investigate the impact of ciprofloxacin (CIP) and metronidazole (MTZ) on gut microbiota structure/function in a healthy donor and a new-onset therapeutic naïve IBD patient. We also investigate the use of autologous faecal microbiota transplant (aFMT) as a microbial therapeutic to assist recovery of the gut ecosystem. Methods Donor faecal specimens (IBD or healthy) were used to inoculate twin chemostat vessels. Following community stabilisation, vessels were dosed daily with CIP or MTZ for 7 days. In the healthy donor vessel, 4 doses of aFMT were administered over 7 days (post antibiotics) followed by a 7-day recovery. In the IBD vessels no FMT was administered. Vessels were sampled daily to monitor microbial profiles (16S rRNA sequencing) and short chain fatty acid (SCFA) profiles (GC-MS). Results CIP in the healthy vessel caused a significant depletion of acetate, butyrate and propionate, remaining significantly depleted during aFMT. CIP dosing also resulted in an enrichment of Escherichia-Shigella and Enterobacter and a loss of Akkermansia, Faecalibacterium and Bacteroides. During recovery acetate and propionate recovered whilst butyrate remained depleted (p=0.004). In the healthy MTZ vessel, acetate increased during antibiotic dosing and was restored following aFMT. Butyrate decreased during MTZ dosing and remained significantly decreased (p<0.031). Propionate levels also reduced following MTZ but were found in highest abundance during aFMT and recovery phases (p<0.038). Parabacteroides increased during MTZ dosing which subsequently decreased to stable abundance. Akkermansia was significantly increased in recovery phase compared to aFMT and stability phases. In the IBD donor, CIP caused a significant reduction in 10 SCFAs and several taxa including Bacteroides and Dialister genera alongside an enrichment of Enterococcus, Lachnospiraceae, Anaerofilum and Eisenbergiella. MTZ treatment resulted in a less dramatic reduction in SCFAs. Microbial community changes included an enrichment of Escherichia-Shigella, Enterococcus and Parasutterella and a depletion of Alistipes and Dialister. Conclusion CIP and MTZ significantly depleted levels of beneficial genera and reduced the abundance of SCFAs. Neither donor community was able to fully restore composition or function. aFMT modulated the healthy donor microbial communities to a "new healthy". Further investigation into long-term antibiotic treatment and the use of microbial therapeutics is needed to fully understand this relationship in the context of IBD.

Impact sculpting of the early martian atmosphere.

Intense bombardment of solar system planets in the immediate aftermath of protoplanetary disk dissipation has played a key role in their atmospheric evolution. During this epoch, energetic collisions will have removed substantial masses of gas from rocky planet atmospheres. Noble gases are powerful tracers of this early atmospheric history, xenon in particular, which on Mars and Earth shows significant depletions and isotopic fractionations relative to the lighter noble gasses. To evaluate the effect of impacts on the loss and fractionation of xenon, we measure its ionization and recombination efficiency by laser shock and apply these constraints to model impact-driven atmospheric escape on Mars. We demonstrate that impact bombardment within the first 200 to 300 million years of solar system history generates the observed Xe depletion and isotope fractionation of the modern martian atmosphere. This process may also explain the Xe depletion recorded in Earth's deep mantle and provides a latest date for the timing of giant planet instability.

Geochronology and Geochemistry of the Uhelchulu Quartz Diorite-Granodiorite in Inner Mongolia of China: Implications for Evolution of the Hegenshan Ocean in the Early-Middle Devonian

The Uhelchulu quartz diorite-granodiorite intrusions in Xiwuqi, Inner Mongolia, are exposed along the northwestern margin of the Xilinhot microcontinental block, located within the central and eastern parts of the southeastern Hegenshan suture zone. LA-ICP-MS zircon U-Pb dating yielded crystallization ages of (396 ± 8) Ma for the quartz diorite and (385 ± 5) Ma for the granodiorite, indicating an Early-Middle Devonian magmatic event. The quartz diorite exhibits I-type granite features, characterized by elevated Al2O3 (14.33–15.43 wt%), MgO (3.73–5.62 wt%), and Na (Na2O/K2O = 1.04–1.44), coupled with low P2O5 (0.15–0.20 wt%) and TiO2 (0.73–0.99 wt%). Trace element patterns show relative enrichments in Rb, Th, U, and Pb, while Nb, Ta, Sr and Ti are relatively depleted. Total REE contents are relatively low (123–178 ppm), with significant LREE enrichment (ΣLREE/ΣHREE = 4.75–5.20), and a non-obvious Eu anomaly (δEu = 0.75–0.84). In contrast, the granodiorite displays S-type granite characteristics, with high SiO2 (70.48–73.01 wt%), K (K2O/Na2O = 1.35–1.83), Al2O3 (A/CNK = 1.16–1.31), and a high differentiation index (DI = 76–82). Notably, MgO (1.44–2.24 wt%) contents are low, and significant depletions of Ba, Sr, Ti, and Eu are observed, while Rb, Pb, Th, U, Zr, and Hf are significantly enriched. Total REE contents are relatively low (178–314 ppm), exhibiting significant LREE enrichment (LREE/HREE = 6.17–8.36) and a pronounced negative Eu anomaly (δEu = 0.34–0.49). The overall characteristics point towards an active continental margin arc background for the Uhelchulu intrusions. Previous studies have suggested that the Hegenshan ocean continuously subducted northward from the Early Carboniferous to the Late Permian, but there is a lack of evidence for its geological evolution during the pre-Early Carboniferous. Therefore, this paper provides a certain basis for studying the geological evolution during the pre-Early Carboniferous in the Hegenshan ocean. We preliminarily believed that the Hegenshan ocean underwent a southward subduction towards the Xilinhot microcontinental block in the Xiwuqi area, at least from the Early Devonian to the Middle Devonian and the Hegenshan ocean may might have undergone a shift in subduction mechanism during the Late Devonian or Early Carboniferous.

Therapeutics of L. Lanceolata and V. Doniana on Alcohol Induced Hepatic Pathology

Additive and stimulative properties of alcohol encourage large intake. Notwithstanding, chronic alcohol consumption causes severe hepatic damage, that alters normal biological function of the liver. The aim of this research was to assess the effects of Lophira lanceolata and Vitex doniana extracts on alcohol induced hepatotoxicity using rat model by examining liver serum enzymes (AST, ALT, ALP), lipid peroxidation level, antioxidant enzyme activity and haematological parameters. Thirty Wister rats weighing 70 to 120g were placed in six groups of fives per group. The negative control and treatment groups were familiarized with alcohol (42%) in drinking water before oral administration of 42% consumable alcohol (v/v, 1ml/100g body weight) for nine days. Afterward, the treatment groups were administered 600mg/kg body weight extracts of Lophira lanceolata, Vitex doniana and Vitamin C (as standard drug). Body weight and behavioural characters were monitored during the period. It was observed that, rats in the negative and treatment groups showed significant depletion in weight and negative changes in behaviour. Furthermore, biochemical assessment revealed significant elevated levels of liver serum enzymes (ALT, AST, ALP) activity, total and direct bilirubin, MDA, SOD and CAT while GSH and haematological parameters were significantly depleted at p<0.05. However, the treated groups revealed significant reduction in the liver enzymes, MDA, SOD and CAT while GSH activity and haematological parameters were significantly increased. In conclusion, both extracts have ameliorative and antioxidant properties that could mitigates chronic alcohol hepatotoxicity.

Metagenomic assessment of gut microbial communities and risk of severe COVID-19

BackgroundThe gut microbiome is a critical modulator of host immunity and is linked to the immune response to respiratory viral infections. However, few studies have gone beyond describing broad compositional alterations in severe COVID-19, defined as acute respiratory or other organ failure.MethodsWe profiled 127 hospitalized patients with COVID-19 (n = 79 with severe COVID-19 and 48 with moderate) who collectively provided 241 stool samples from April 2020 to May 2021 to identify links between COVID-19 severity and gut microbial taxa, their biochemical pathways, and stool metabolites.ResultsForty-eight species were associated with severe disease after accounting for antibiotic use, age, sex, and various comorbidities. These included significant in-hospital depletions of Fusicatenibacter saccharivorans and Roseburia hominis, each previously linked to post-acute COVID syndrome or “long COVID,” suggesting these microbes may serve as early biomarkers for the eventual development of long COVID. A random forest classifier achieved excellent performance when tasked with classifying whether stool was obtained from patients with severe vs. moderate COVID-19, a finding that was externally validated in an independent cohort. Dedicated network analyses demonstrated fragile microbial ecology in severe disease, characterized by fracturing of clusters and reduced negative selection. We also observed shifts in predicted stool metabolite pools, implicating perturbed bile acid metabolism in severe disease.ConclusionsHere, we show that the gut microbiome differentiates individuals with a more severe disease course after infection with COVID-19 and offer several tractable and biologically plausible mechanisms through which gut microbial communities may influence COVID-19 disease course. Further studies are needed to expand upon these observations to better leverage the gut microbiome as a potential biomarker for disease severity and as a target for therapeutic intervention.

Ferroptosis-Mediated Cell Death Induced by NCX4040, The Non-Steroidal Nitric Oxide Donor, in Human Colorectal Cancer Cells: Implications in Therapy.

Our recent studies show that the treatment of human ovarian tumor cells with NCX4040 results in significant depletions of cellular glutathione, the formation of reactive oxygen/nitrogen species and cell death. NCX4040 is also cytotoxic to several human colorectal cancer (CRC) cells in vitro and in vivo. Here, we examined the ferroptosis-dependent mechanism(s) of cytotoxicity of NCX4040 in HT-29 and K-RAS mutant HCT 116 colon cell lines. Ferroptosis is characterized by the accumulation of reactive oxygen species (ROS) within the cell, leading to an iron-dependent oxidative stress-mediated cell death. However, its relevance in the mechanism of NCX4040 cytotoxicity in CRCs is not known. We found that NCX4040 generates ROS in CRC cells without any depletion of cellular GSH. Combinations of NCX4040 with erastin (ER) or RSL3 (RAS-selective lethal 3), known inducers of ferroptosis, enhanced CRC death. In contrast, ferrostatin-1, an inhibitor of ferroptosis, significantly inhibited NCX4040-induced cell death. Treatment of CRC cells with NCX4040 resulted in the induction of lipid peroxidation in a dose- and time-dependent manner. NCX4040 treatment induced several genes related to ferroptosis (e.g., CHAC1, GPX4 and NOX4) in both cell lines. Metabolomic studies also indicated significant increases in both lipid and energy metabolism following the drug treatment in HT-29 and HCT 116 cells. These observations strongly suggest that NCX4040 causes the ferroptosis-mediated cell death of CRC cells. Furthermore, combinations of NCX4040 and ER or RSL3 may contribute significantly to the treatment of CRC, including those that are difficult to treat due to the presence of Ras mutations in the clinic. NCX4040-induced ferroptosis may also be a dynamic form of cell death for the treatment of other cancers.

M92 (NGC 6341) Is a Metal-complex Globular Cluster with an Atypical Primordial Population

We present a multiple stellar population study of the metal-poor globular cluster (GC) M92 (NGC 6341), which is long known for the substantial metallicity dispersion, using our own photometric system. We find two groups with slightly different mean metallicities, the metal-poor (MP) stars with [Fe/H] hk = −2.412 ± 0.03 and the metal-rich (MR) ones with −2.282 ± 0.002. The MP constitutes about 23% of the total mass with a more central concentration. Our populational tagging based on the [C/Fe] ch and [N/Fe] nh provides the mean n(P):n(I):n(E) = 32.2:31.6:36.2 (±2.4), where P, I, and E denote the primordial, intermediate, and extreme populations, respectively. Our populational number ratio is consistent with those of others. However, the MP has a significantly different populational number ratio than the mean value, and the domination of the primordial population in the MP is consistent with observations of Galactic GCs that less massive GCs contain larger fractions of the primordial population. Structural and constituent differences between the MP and MR may indicate that M92 is a merger remnant in a dwarf galaxy environment, consistent with recent suggestions that M92 is a GC in a dwarf galaxy or a remnant nucleus of the progenitor galaxy. Discrepancy between our method and those widely used for the Hubble Space Telescope photometry exists in the primordial population. Significant magnesium and oxygen depletions of −0.8 and −0.3 dex, respectively, and helium enhancement of ΔY ≳ 0.03 are required to explain the presence of this abnormal primordial group. No clear explanation is available with limited information of detailed elemental abundances.

PGE geochemical and Os-S-Sr-Nd isotopic constraints on the genesis of the Shitoukengde magmatic sulfide deposit in the East Kunlun Orogenic Belt, NW China

Numerous early Paleozoic mafic–ultramafic intrusions that host important Ni-Cu sulfide mineralization occur in the northern part of the Qinghai-Tibet Plateau, NW China. The sulfide-bearing Shitoukengde Ni-Cu deposit, which is hosted in a ∼424 Ma mafic–ultramafic intrusion, is one of the most represent magmatic sulfide deposits in the East Kunlun Orogenic Belt. The intrusion is composed of a large gabbroic body, cut by lherzolite, olivine websterite, websterite, and minor dunite. Disseminated and net-textured sulfide mineralization occurs as lenses in the center and towards the bottom of the ultramafic bodies. The sulfide mineralization has variable sulfur contents of 0.3 to 13.6 wt% with Ni grade of 0.41 wt% on average, and low platinum-group element (PGE) concentrations (<8.2 ppb Ru and <11.9 ppb Pd). Pd and Ru tenors of bulk sulfides in the deposit are characterized by a positive correlation, indicating that PGE tenor variations are mainly controlled by varying R-factors (magma/sulfide mass ratio). The estimated PGE concentrations in the Shitoukengde magma are about three orders of magnitude lower than those in continental tholeiites. The significant PGE depletions in the Shitoukengde deposit may be due to sulfide removal in the magma conduit at depth. Our new sulfur isotope data reported range in δ34S values from −5.0 to 4.9‰ for the sulfide minerals of the Shitoukengde deposit, clearly distinct from those of mantle-derived sulfur (0 ± 2‰) and immediate country rocks (1.1 to 2.0‰). This, together with the modeling for S isotope variation as a function of R-factor, suggest that the current δ34S range is attributed to the addition of external sulfides with anomalous δ34S into the magma at depth, followed by progressive modification to crustal signature by uncontaminated pulses of magma. The ultramafic rocks exhibit low εNd(t) values (−4.71 to 2.12) and high (87Sr/86Sr)i ratios (0.7073 to 0.7130), which are more enriched relative to the isotopic compositions of depleted mantle. Mixing calculations indicate that about 15 to 20 wt% of lower crustal contamination plus variable upper crustal contamination is required to explain the variations of Sr-Nd isotopes in the Shitoukengde intrusion. Significant crustal contamination is also supported by the mineralization at Shitoukengde having high (187Os/188Os)i (0.24 to 4.51) and γOs(t) values (0.24 to 2534) that are comparable to or higher than those of the important Ni-Cu deposits worldwide. Current results provide evidence that assimilation of crustal sulfur at deep crust and siliceous material with variable crustal contamination during magma ascent and emplacement played critical roles in the attainment of sulfide saturation in the parental magma and the formation of the Shitoukengde deposit.

Apolipoprotein A-I Synthesis and Secretion Are Increased in Hep G2 Cells Depleted of Copper by Cupruretic Tetramine

Two amine chelators, namely N,N′-bis(2-aminoethyl)-1,3-propanediamine-4 HCl (2,3,2-tetramine) and diamsar, were used at various levels to deplete copper (Cu) from cultured Hep G2 cells. For cells cultured at 0.63 µmol of Cu/L, maximal depletions were attained after 24 h of incubation with 10 µmol of either chelator/L, which resulted in an average significant depletion of 45% of cellular Cu. In addition, when cells were cultured continuously for four passages at 0.63 µmol Cu/L with 20 µmol of 2,3,2-tetramine/L, cellular Cu was significantly depleted more than 40% compared with controls. Furthermore, after two passages of 2,3,2-tetramine treatment, cells were pulsed for 10 min with [3H]leucine and chased up to 2 h. At the end of the pulse, the amount of [3H]leucine incorporated into apolipoprotein A-I was twofold greater (P < 0.05) in treated than in control cells. No difference was detected for the synthesis of apolipoprotein B and total protein. During the chase, the cellular depletion curves for apolipoprotein A-I, apolipoprotein B and total protein were not altered by 2,3,2-tetramine treatment. From 30 to 120 min of the chase, the amount of nascent apolipoprotein A-I degraded was small and not altered, but that secreted into the medium was 56% higher (P < 0.05) in the Cu-depleted than in the control cells.

GARP dysfunction results in COPI displacement, depletion of Golgi v-SNAREs and calcium homeostasis proteins.

Golgi-associated retrograde protein (GARP) is an evolutionary conserved heterotetrameric protein complex that tethers endosome-derived vesicles and is vital for Golgi glycosylation. Microscopy and proteomic approaches were employed to investigate defects in Golgi physiology in RPE1 cells depleted for the GARP complex. Both cis and trans-Golgi compartments were significantly enlarged in GARP-knock-out (KO) cells. Proteomic analysis of Golgi-enriched membranes revealed significant depletion of a subset of Golgi residents, including Ca2+ binding proteins, enzymes, and SNAREs. Validation of proteomics studies revealed that SDF4 and ATP2C1, related to Golgi calcium homeostasis, as well as intra-Golgi v-SNAREs GOSR1 and BET1L, were significantly depleted in GARP-KO cells. Finding that GARP-KO is more deleterious to Golgi physiology than deletion of GARP-sensitive v-SNAREs, prompted a detailed investigation of COPI trafficking machinery. We discovered that in GARP-KO cells COPI is significantly displaced from the Golgi and partially relocalized to the ER-Golgi intermediate compartment (ERGIC). Moreover, COPI accessory proteins GOLPH3, ARFGAP1, GBF1, and BIG1 are also relocated to off-Golgi compartments. We propose that the dysregulation of COPI machinery, along with the depletion of Golgi v-SNAREs and alteration of Golgi Ca2+ homeostasis, are the major driving factors for the depletion of Golgi resident proteins, structural alterations, and glycosylation defects in GARP deficient cells.

SARS CoV-2 mRNA vaccination exposes latent HIV to Nef-specific CD8+ T-cells

Efforts to cure HIV have focused on reactivating latent proviruses to enable elimination by CD8+ cytotoxic T-cells. Clinical studies of latency reversing agents (LRA) in antiretroviral therapy (ART)-treated individuals have shown increases in HIV transcription, but without reductions in virologic measures, or evidence that HIV-specific CD8+ T-cells were productively engaged. Here, we show that the SARS-CoV-2 mRNA vaccine BNT162b2 activates the RIG-I/TLR – TNF – NFκb axis, resulting in transcription of HIV proviruses with minimal perturbations of T-cell activation and host transcription. T-cells specific for the early gene-product HIV-Nef uniquely increased in frequency and acquired effector function (granzyme-B) in ART-treated individuals following SARS-CoV-2 mRNA vaccination. These parameters of CD8+ T-cell induction correlated with significant decreases in cell-associated HIV mRNA, suggesting killing or suppression of cells transcribing HIV. Thus, we report the observation of an intervention-induced reduction in a measure of HIV persistence, accompanied by precise immune correlates, in ART-suppressed individuals. However, we did not observe significant depletions of intact proviruses, underscoring challenges to achieving (or measuring) HIV reservoir reductions. Overall, our results support prioritizing the measurement of granzyme-B-producing Nef-specific responses in latency reversal studies and add impetus to developing HIV-targeted mRNA therapeutic vaccines that leverage built-in LRA activity.

Jupiter's Low-Altitude Auroral Zones: Fields, Particles, Plasma Waves, and Density Depletions.

The Juno spacecraft's polar orbits have enabled direct sampling of Jupiter's low‐altitude auroral field lines. While various data sets have identified unique features over Jupiter's main aurora, they are yet to be analyzed altogether to determine how they can be reconciled and fit into the bigger picture of Jupiter's auroral generation mechanisms. Jupiter's main aurora has been classified into distinct “zones”, based on repeatable signatures found in energetic electron and proton spectra. We combine fields, particles, and plasma wave data sets to analyze Zone‐I and Zone‐II, which are suggested to carry upward and downward field‐aligned currents, respectively. We find Zone‐I to have well‐defined boundaries across all data sets. H+ and/or H3 + cyclotron waves are commonly observed in Zone‐I in the presence of energetic upward H+ beams and downward energetic electron beams. Zone‐II, on the other hand, does not have a clear poleward boundary with the polar cap, and its signatures are more sporadic. Large‐amplitude solitary waves, which are reminiscent of those ubiquitous in Earth's downward current region, are a key feature of Zone‐II. Alfvénic fluctuations are most prominent in the diffuse aurora and are repeatedly found to diminish in Zone‐I and Zone‐II, likely due to dissipation, at higher altitudes, to energize auroral electrons. Finally, we identify significant electron density depletions, by up to 2 orders of magnitude, in Zone‐I, and discuss their important implications for the development of parallel potentials, Alfvénic dissipation, and radio wave generation.

Sedimentary Black Carbon Isotope Record of Holocene Climate Changes on the Northeastern Tibetan Plateau

AbstractThe northeastern Tibetan Plateau (TP) is an unique area for studying the interactions between the Asian Monsoonal climate and the westerlies. However, significant contraries on Holocene paleoclimate evolution are still exist. To better understand the climate history on the northeastern TP particularly its variability, we here investigated the stable carbon isotope compositions of Black Carbon (BC) (δ13CBC) in Lake Gyaring sediment core from the source area of the Yellow River. The δ13CBC values were relatively positive before ∼6.2 cal kyr BP indicating that the climate on northeastern TP was warm and dry during the first half of the Holocene, while the decreasing trend thereafter reflects that the climate on the northeastern TP has become generally cooler and wetter since the middle Holocene. Superimposed on the long‐term paleoclimate evolution pattern, at least seven centennial‐scale cold‐wet events characterized by significant δ13CBC depletions occurred at about 8.4, 7.5, 6.2, 5.0, 4.3, 2.7, and 0.9 cal kyr BP. Those abrupt climate events well corresponded to the weak Asian Monsoon episodes and the ice‐rafted debris records in the North Atlantic. We speculate that the cold climate signals from the North Atlantic resulted from the sea‐ice expansion have been transported to the northeastern TP through the westerlies leading to a cold conditions there. Besides, the anomalous easterly water vapor transportation over the northern periphery of the TP during weak monsoon intervals has generated the wet conditions on the northeastern TP.

A mouse infection model and long-term lymphatic endothelium co-culture system to evaluate drugs against adult Brugia malayi.

The development of new drugs targeting adult-stage lymphatic filarial nematodes is hindered by the lack of a robust long-term in vitro culture model. Testing potential direct-acting and anti-Wolbachia therapeutic candidates against adult lymphatic filariae in vitro requires their propagation via chronic infection of gerbils. We evaluated Brugia malayi parasite burden data from male Mongolian gerbils compared with two immune-deficient mouse strains highly susceptible to B. malayi: CB.17 Severe-Combined Immmuno-Deficient (SCID) and interleukin-4 receptor alpha, interleukin-5 double knockout (IL-4Rα-/-IL-5-/-) mice. Adult worms generated in IL-4Rα-/-IL-5-/- mice were tested with different feeder cells (human embryonic kidney cells, human adult dermal lymphatic endothelial cells and human THP-1 monocyte differentiated macrophages) and comparative cell-free conditions to optimise and validate a long-term in vitro culture system. Cultured parasites were compared against those isolated from mice using motility scoring, metabolic viability assay (MTT), ex vivo microfilariae release assay and Wolbachia content by qPCR. A selected culture system was validated as a drug screen using reference anti-Wolbachia (doxycycline, ABBV-4083 / flubentylosin) or direct-acting compounds (flubendazole, suramin). BALB/c IL-4Rα-/-IL-5-/- or CB.17 SCID mice were superior to Mongolian gerbils in generating adult worms and supporting in vivo persistence for periods of up to 52 weeks. Adult females retrieved from BALB/c IL-4Rα-/-IL-5-/- mice could be cultured for up to 21 days in the presence of a lymphatic endothelial cell co-culture system with comparable motility, metabolic activity and Wolbachia titres to those maintained in vivo. Drug studies confirmed significant Wolbachia depletions or direct macrofilaricidal activities could be discerned when female B. malayi were cultured for 14 days. We therefore demonstrate a novel methodology to generate adult B. malayi in vivo and accurately evaluate drug efficacy ex vivo which may be adopted for drug screening with the dual benefit of reducing overall animal use and improving anti-filarial drug development.

Coseismic fluid–rock interactions in the Yingxiu–Beichuan surface rupture zone of the Mw 7.9 Wenchuan earthquake and their implications for the structural diagenesis of fault rocks

The mechanisms by which fluids modify the mineralogy and geochemistry of fault zones and the role of rock–fluid interactions in fault weakening are subjects of debate. We assessed the mineralogical and geochemical variations in fault rocks in the Shaba area of the Yingxiu–Beichuan surface rupture zone of the Mw 7.9 Wenchuan earthquake using analyses of their mineralogical compositions, major elements, and the microstructural characteristics of wall rocks, damage zones, and oriented fault gouge samples from the principal slip zones. The elemental and mineralogical compositions show a certain regularity across the fault zone from the wall rocks to the fault gouge so that (1) Al2O3, Fe2O3T, and K2O contents increase and exhibit significant enrichments in the fault gouge; (2) Na2O and P2O5 contents decrease gradually and exhibit significant depletions in the fault gouge; (3) quartz, feldspar, and carbonate mineral contents decline; and (4) clay mineral contents increase dramatically. Isocon analysis indicates that mass losses in the Shaba fault zone occur with losses in the damage zone < the fault gouge. The mechanisms of material loss and transformation in the fault zone are complex, and mechanical fracturing, dehydration reactions, and fluid–rock interactions are important factors in changing and controlling the material compositions and fault zone evolution, with material losses and transformation especially important within the fault core. Moreover, due to the damage zone having a higher permeability than the fault core, it was conducive to hydrothermal upwelling, fluid–rock interactions, and fracture healing.

Basalt from the Extinct Spreading Center in the West Philippine Basin: New Geochemical Results and Their Petrologic and Tectonic Implications

We present geological, bulk-rock geochemical and Sr–Nd–Hf isotopic data for mafic rocks from the West Philippine Basin (WPB). These mafic rocks comprise pillow basalts characterized by a vesicular structure. The mid-ocean ridge basalt (MORB)-normalized trace element patterns of basalts from the study area display depletions in Nb. In addition, the chondrite-normalized lanthanide patterns of basalts from the WPB are characterized by significant depletions in the light lanthanides and nearly flat Eu to Lu segments. The investigated rocks have initial 87Sr/86Sr ratios (87Sr/86Sr(i)) of 0.703339–0.703455 and high εNd(t) values (8.0 to 8.7). Furthermore, basalts from the WPB have 176Hf/177Hf ratios that range from 0.28318 to 0.28321 and high εHf(t) from 15.2 to 16.3. Semi-quantitative modeling demonstrates that the parental melts of basalts from the study area were derived by ~20% adiabatic decompression melting of a rising spinel-bearing peridotite source. The Sr–Nd–Hf isotopic compositions of basalts from the WPB indicate that their parental magmas were derived from an upper mantle reservoir possessing the so-called Indian-type isotopic anomaly. Interpretation of the isotopic data suggests that the inferred mantle source was most likely influenced by minor inputs of a sediment melt derived from a downgoing lithospheric slab. Collectively, the petrographic and geochemical characteristics of basalts from the study area are analogous to those of mafic rocks with a back-arc basin (BAB)-like affinity. As such, the petrogenesis of basalts from the WPB can be linked to upwelling of an Indian-type mantle source due to lithospheric slab subduction that was followed by back-arc spreading.

A Lithosphere–Atmosphere–Ionosphere Coupling Phenomenon Observed Before M 7.7 Jamaica Earthquake

The satellite-based earth observations have become an appropriate instrumental approach in monitoring the natural hazards among modern-day researchers. This study presents a multi-parameter approach using precursors of different physical nature defining the states of atmosphere and the ionosphere in terms of temporal and spatial variations about 5 days before the impending M 7.7 Jamaican earthquake (EQ). We performed a comprehensive analysis from the surface to the ionosphere at different altitude levels by analyzing the different datasets comprising, surface air temperature, relative humidity, total column water vapor, air pressure, Outgoing Longwave Radiations (OLR), and the total electron content of the global ionosphere maps (GIM-TEC). We observed a sharp increment in the atmospheric chemical potential (ACP) due to the increased radon activity that led to an abrupt decrement in the atmospheric relative humidity and, consequently, increased OLR that provides strong evidence of the air ionization production around the epicenter of M 7.7 EQ. Moreover, to check the periodicity of these atmospheric parameters, we performed a confutation analysis by meticulously analyzing these parameters in the same month and region for the previous 5 years during the non-existence of any major seismicity. This technique confirmed that the simultaneous atmospheric variations observed before the Jamaica EQ are not cyclic in the absence of significant seismic activities. The ionospheric conditions have also shown consistency with atmospheric disturbances, as depletions in GIM-TECs, having an amplitude of 4 TECU, are observed over the epicenter for 6 h (LT = 13–19 (-– UT)) on January 23, 2020. Additionally, the vertical ionospheric and atmospheric profiles from FORMOSAT-7/COSMIC-2 (F7/C2), at different altitudes (75–225 km) over the EQ epicenter, showed significant depletions on January 23, 2020. These TEC variations are observed to be an effect of the vertical seismogenic electric field due to the production of the air ionization at the atmospheric boundary layer by increased radon activity around the seismic preparation zone. The existence of these co-located synchronized atmospheric and ionospheric anomalies is explicitly and persistently local over a small region of the epicenter of Jamaican EQ that could be considered as potential short-term precursors. Also, these multi-observed anomalies will contribute to the physical explanation of the Lithosphere-Atmosphere–Ionosphere Coupling (LAIC) model.

Stand age and precipitation affect deep soil water depletion of economical forest in the loess area

Success of afforestation efforts hinges on better understanding of roles of water in the deep unsaturated zone in plant water uptake. But previous studies have deep soil water measurement either not deep, or not long enough to understand annual deep soil depletion rate for reforested forests, especially the effect of stand age or precipitation year on annual deep soil water depletion rate. To address this question, we selected, in 2016, four apple orchards planted on cropland in 2008, 2005, 2001, and 1994 (i.e., young orchard AP2008, mature orchards AP2005 and AP2001, and old orchard AP1994, respectively) in a thick loess area. Soil water contents were measured in 2016 - 2020 to the depth of 19 m with an in-situ neutron probe technique. Meanwhile, leaf area indexes were measured in 2017 and 2019 to represent each orchard water demand. Results showed annual deep soil water in 5-19 m showed a peak depletion rate of 160 mm yr−1 at around 14 years of orchard age. After the age effects are removed, mature orchards AP2005 and AP2001 showed significant depletions in 5-19 m deep soil water in the dry year 2016; in normal years 2017/2018, the depletion rates were reduced; and no significant deep soil water depletions were found in all orchards in the wet year 2019. The greater deep soil water uses in mature orchards AP2005 and AP2008 agreed with greater leaf area indexes. The depleted deep soil water contributions to actual evapotranspiration (AET) were 27.0% and 13.6% in the dry year 2016 for orchards AP2005 and AP2001, respectively; but their respective contributions were 15.1% and 7.9% in normal years 2017/2018. This indicates both the rainwater supply and the stand age affect deep soil water use, which suggests that more rainwater saving strategies and branch pruning are needed to avoid excess deep soil water depletion in dry and normal precipitation years for the mature forest on the Chinese Loess Plateau.

Sub-acute exposure to Sudan IV-adulterated palm oil induces oxidative stress and represses the expression of Nrf2 and antioxidant genes in male albino rats

This study investigated the effects of Sudan IV dye (S4D) on antioxidant biomarkers using palm oil adulterated with S4D. Thirty male albino rats were grouped into five (n = 6); Normal control, palm oil (PO), PO + S4D (100 mg/kg), PO + S4D (250 mg/kg), and S4D (250 mg/kg) for 21 days. Oxidative stress biomarkers were assessed in the serum, liver, and kidneys. Exposure to S4D (alone and in adulterated PO) occasioned significant depletions in the activities of SOD, CAT, and GPx, as well as GSH levels in the assessed compartments. Contrastingly, the levels of NO and MDA were significantly (p < 0.05) increased in the serum, liver, and kidney of rats exposed to PO + S4D (both doses) and S4D (250 mg/kg) when compared to control rats. Further, the expressions of the genes coding for CAT, GPx-1, GSR, and Nrf-2 were significantly (p < 0.05) down-regulated, relative to β-actin, in groups exposed to S4D compared to the control. Interestingly, these parameters were not significantly different (p > 0.05) in the unadulterated PO-exposed rats compared to the control. These results show that S4D depleted the antioxidant capacities, while potentiating the generation of reactive species and oxidative damage. This study provides useful information on the oxidative mechanisms associated with consumption of S4D-containing consumer products.

Biogeochemical thallium cycling during a mesocosm phytoplankton spring bloom: Biotic versus abiotic drivers

Thallium (Tl) is classified as a non-(bio)-essential and highly toxic element in the marine environment. Despite its active and passive involvement in bio-cycling processes, it is considered a conservative type element in open ocean settings. Previous studies on the Tl-behavior in the coastal waters of the southern North Sea, however, documented non-conservative Tl-behavior in seasonal and tidal patterns. As drivers for the non-conservative depletion, Tl-fixation in redox stratified adjacent sediments as well as its complexation with algae-bloom derived organic matter were suggested. Due to superimposition by resuspended lithogenic particles, it was not possible to distinguish whether the Tl concentration pattern was induced by biotic or abiotic processes. The main motivation of the present study was to investigate the non-conservative Tl-behavior during a phytoplankton bloom in coastal ocean water masses and to identify potential key drivers. We conducted an indoor mesocosm experiment where artificial seawater was inoculated with a natural phytoplankton and bacteria community from the southern North Sea and incubated under natural light and temperature conditions, mimicking a neritic North Sea water column. The incubation of six weeks covered the different stages of two distinct phytoplankton bloom events as well as a subsequent bacteria bloom. Our results reveal a non-conservative Tl-depletion, which seemed to be primarily caused by the coupling to algae bloom derived OM-cycling. The extent of Tl-depletion was dependent on the amount and the composition of organic matter. While the first phytoplankton bloom, dominated by Diatom-species, did not induce significant deviations of Tl from theoretical conservative behavior, especially the colonial stage (hydrogel formation) of the secondary occurring Phaeocystis sp. bloom induced significant depletions of dissolved Tl with rates up to ~27% d-1. Global extrapolations of potential algae-induced deficits in dissolved Tl and its potential export Tl from the open water column have shown that the processes identified for Tl removal in this study could be responsible for a flux in the range of 4–20% of the total removal previously assumed in Tl mass balances. Our study emphasizes that although Tl is classified as a conservative-type element, biological processes have an impact on the global Tl budget and thus must be considered in the respective oceanographic models.