In our search for bioactive compounds from North African plants [1, 2], we found that the Tunisian medicinal plant Arthrocnemum glaucum Delile inhibited adipogenesis in 3T3-L1. A. glaucum, which belongs to the Chenopodiaceae, is a densely branched robust plant that grows wild in the Sahara Desert of Tunisia, especially in the Mediterranean Coastal region. It was previously reported that some alkaloids and some sterols were isolated from A. glaucum [3–6]. In this research, the bioassay-guided fractionation of this plant implied that the bioactive components are two isorhamnetin glycosides, isorhamnetin-3-O-[apiosyl(1 6)]glucosyl-7-O-rhamnoside (1) and isorhamnetin-3-O-rutinoside (2). In this paper, we report the isolation and structure elucidation of the two isorhamnetin glycosides 1 and 2 from aerial parts of this plant and their antiadipogenesis activity. The aerial part of A. glaucum was collected at the Sahara Desert of Tunisia. The voucher specimen (TPM-20080401) is maintained at Alliance for Research on North Africa (ARENA), University of Tsukuba and Environmental Bioprocesses Laboratory at Biotechnology Center of Sfax. The leaves were dried in the shade at 25 C. The dried leaves of A. glaucum (100 g) were extracted with MeOH. The MeOH extracts were partitioned between EtOAc and H2O, and the H2O layer was partitioned with n-BuOH. The n-BuOH-soluble portion (1.33 g) was subjected to ODS column chromatography and purified by reversed-phase HPLC or gel-filtration HPLC to afford two known flavonol glycosides, isorhamnetin-3-O[apiosyl(1 6)]glucosyl-7-O-rhamnoside (1, 0.0085%) and isorhamnetin-3-O-rutinoside (2, 0.0027%). Structure elucidation of 1 and 2 was through spectroscopic data, mainly 1H and 13C NMR spectra and ESI-MS spectra. Full assignment of signals in the NMR spectra of 1 and 2 was confirmed by 2D NMR experiments (1H–1H COSY, NOESY, HMQC, and HMBC) and comparison with reported data [7, 8].