The effect of segregation defect of SiC particles on the properties of materials was studied. 15% SiCp/2009Al composites were prepared by powder metallurgy (PM). Special SiC/Al samples were added to 15% SiCp/2009Al composites. These SiC/Al samples with different sizes and volume fractions were 25%, 35%, 45% and 60%, respectively, which resulted in SiC particulates segregation defect. The 15% SiCp/2009Al composites with defects were tested by ultrasonic testing. Tensile samples were obtained at the locations, where defects might be detected and the mechanical properties were tested. The results showed that all defective samples were cracked at the defective location. The difference in tensile strength between the samples of defect and the samples without defect was large. The toughness of the sample containing the defect reduced and the brittleness increased. The dimples on the matrix indicate that ductile fracture occurred during the fracture process. The cleavage fracture or cracking of the SiC particulates indicated that the stress can be effectively transferred from the matrix to the particles, and the particulates strengthen the matrix well. However, the sample with defect led to brittle fracture in the defect, and a crack source produced at the interface, resulting in a significant decrease in the mechanical properties of the material. If the inhomogeneous distribution of particulate containing a large area was found in the ultrasonic testing of the aluminum matrix composites, the tensile properties of the products generally cannot meet the requirement for application.