Shot Line Research Articles

Increased Availability of Tryptophan in 5-Methyltryptophan-Tolerant Shoots of Catharanthus roseus and Their Postharvest in vivo Elicitation Induces Enhanced Vindoline Production

Ten 5-methyltryprophan (5-MT)-resistant multiple shoot culture lines in three genotypes of Catharanthus roseus were selected in vitro. The variant shoot lines displayed a differential threshold tolerance limit against the analogue stress, ranged from 20 to 70 mg/l 5-MT in the medium. The lines tolerant to 40 mg/l 5-MT stress were most stable and fast proliferating. All the selected lines in the presence of 5-MT stress recorded increased level of tryptophan in their free amino acid pool. Highest tryptophan accumulation occurred in lines P40, P30, D40, and N40 (i.e., 296.5, 241.0, 200.6, and 202.0 μg/g dry wt., respectively). A concomitant increase in the total alkaloid content (2.3-3.8 % dry wt.) under the analogue stress was also noticed in these lines when compared to 1.0-1.58 % dry wt. in the respective wild-type shoot maintained on a stress-free medium. The HPLC analysis of the alkaloid extracts of the 5-MT-tolerant lines grown under analogue stress also revealed vindoline as a major constituent with maximum accumulation in lines N40, N30, D30, D40, and P40 (0.046, 0.032, 0.034, and 0.022 % dry wt., respectively). The rooted shoots of 5-MT-tolerant lines were successfully acclimatized under glasshouse environment wherein they grew normally and set seeds. Flowering twigs or leaves excised from 1-year-old glasshouse-grown plants of 5-MT variant lines upon postharvest in vivo elicitation with 30 mg/l 5-MT or 5.0 mg/l tryptophan registered an eight-to-tenfold increment in their vindoline content within 24-48 h.

탄두의 관통 효과를 고려한 함정 취약성 평가 절차에 관한 기본 연구

The survivability of warship is assessed by susceptibility, vulnerability and recoverability. Essentially, a vulnerability assessment is a measure of the effectiveness of a warship to resist hostile weapon effects. Considering the shot line and its penetration effect on the warship, present study introduces the procedural aspects of vulnerability assessments of warship. Present study also considers the prediction of penetration damage to a target caused by the impact of projectiles. It reflects the interaction between the weapon and the target from a perspective of vulnerable area method and COVART model. The shotline and tracing calculation have been directly integrated into the vulnerability assessment method based on the penetration equation empirically obtained. A simplified geometric description of the desired target and specification of a threat type is incorporated with the penetration effect. This study describes how to expand the vulnerable area assessment method to the penetration effect. Finally, an example shows that the proposed method can provide the vulnerability parameters of the warship or its component under threat being hit through tracing the shotline path thereby enabling the vulnerability calculation. In addition, the proposed procedure enabling the calculation of the component's multi-hit vulnerability introduces a propulsion system in dealing with redundant Non-overlapping components.

Enhanced vincamine production in selected tryptophan-overproducing shoots of Vinca minor

Vinca minor is the sole source of vincamine, an alkaloid known to be used in a variety of cerebral disorders. Three stable variant shoot lines (V10, V20 and V30) with tolerance thresholds of 10, 20 and 30 mg/l 5-methyltryptophan (5-MT; analogue of tryptophan), respectively, were selected. These lines showed twofold to threefold increase in tryptophan content and 1.5- to 2-fold increment in the total alkaloids in comparison to the wild line shoots. A maximum of 16-fold enhancement in vincamine production was recorded in V30 line followed by eightfold in V20 line. Inter simple sequence repeat (ISSR)-PCR amplification of all the three lines showed total of 65 bands; out of which 60 were monomorphic (92.3 %) and 5 were polymorphic (7.7 %). Tryptophan being a limiting factor in the indole alkaloid pathway plays a crucial role in modulating the flux towards vincamine production and its over-production positively resulted into enhanced vincamine production.

303 ショットライニングと熱処理による炭素鋼への機能性皮膜の形成

303 ショットライニングと熱処理による炭素鋼への機能性皮膜の形成

302 ショットライニングと熱処理による合金鋼への機能性皮膜の形成

302 ショットライニングと熱処理による合金鋼への機能性皮膜の形成

3D imaging challenges in steeply dipping mining structures: New lights on acquisition geometry and processing from the Brunswick no. 6 seismic data, Canada

We have analyzed and processed a [Formula: see text] nonorthogonal 3D surface reflection seismic data in the Brunswick no. 6 area to better understand the effect of acquisition geometry on the resultant image and to provide 3D information about the main geologic structures hosting the mineralization. The 3D data were processed using a conventional prestack dip moveout (DMO) and poststack migration algorithm with special focus on refraction static corrections, velocity analysis, and DMO corrections that are important for the data recorded in crystalline environment. However, the nonorthogonal nature of the 3D data combined with its narrow-azimuth, irregular offset distributions, and 2D nature of midpoint distribution in common depth point bins resulted in a lower quality seismic image than those observed on a series of 2D seismic profiles collected in the area prior to the 3D data acquisition. 2D wavenumber spectrum of the data suggests acquisition footprint associated with the data. Most of the noise associated with the acquisition footprint manifested itself as short-length, high-amplitude shallow reflections but largely were attenuated using a dip filter running in the wavenumber domain. Various bin size and geometries were tested, and the best result was obtained using rectangular bins aligned in the orientation of the shot lines. The processing results indicated that the highly prospective and mineralized Brunswick horizon is part of a continuous reflective package that could guide future deep mineral exploration in this mining camp.

Formation of Fe-Al Intermetallic Compound Film on Carbon Steel by Shot Peening and Heat Treatment

The formation of an Fe-Al intermetallic compound film on carbon steel by shot lining and heat treatment was investigated. In the experiment, a centrifugal-type peening machine with an electrical heater was employed. The shot medium was high-carbon cast steel. The substrate was a commercial carbon steel, and the sheet was commercially available pure aluminum. The shot lining process of carbon steel with an aluminum sheet was carried out at 300 °C in air using a peening machine. Heat treatment was performed at diffusion temperatures from 600 to 1050 °C in vacuum. The lined substrates exhibited a harder layer of Al-rich intermetallics, such as Fe2Al5, in the diffusion temperature range of 650 to 900 °C. When the temperature of the lined substrates was more than 930 °C, the surface was covered with thicker and highly anticorrosive layers of Fe-rich intermetallics, such as FeAl and Fe3Al. We found that the present method could be used for the formation of functional films on carbon steel.

Improved organogenic capacity of shoot cultures from mature pedunculate oak trees through somatic embryogenesis as rejuvenation technique

Theoretically, complete rejuvenation of mature trees should occur through somatic embryogenesis, however, this has not been extensively studied. The main objective of the present study was to increase the efficiency of in vitro clonal propagation for mature Quercus robur (100–300 years old), by induction of somatic embryogenesis as rejuvenation step prior to establishment of shoot culture through micropropagation of somatic embryo-derived plantlets. Shoot culture lines of “mature” origin were established from epicormic shoots of two centenarian oak genotypes (Sainza and CR-0) and maintained by axillary shoot proliferation. Embryogenic lines were also initiated from epicormic leaf explants of the same genotypes and maintained by secondary somatic embryogenesis. Although the frequency of somatic embryo conversion into plantlets was low in pedunculate oak, shoot culture lines could be established and maintained by axillary branching from several germinated somatic embryos. For each genotype and shoot culture line of the two origins (mature tree and somatic plantlets), shoot multiplication rate and elongation as well as rooting ability parameters were compared. Compared with “mature-origin” shoot cultures and after more than one year propagation in vitro, shoot lines established from somatic plantlets produced a significantly higher proportion of elongated, rootable shoots (from 26.0–31.6 to 36.8–40.5%) with increased rooting ability (from 3.3–45.6% to 23.2–89.8%). In the case of 300-year-old Sainza genotype such a high organogenic capacity was similar to shoot cultures initiated from basal sprouts. Basal sprouts are considered as “mature” material that retains juvenile characteristics compared with epicormic shoots forced from crown branches. Somatic embryogenesis only slightly improved plant regeneration of shoot cultures from basal sprouts, thus validating their use as “juvenile control”. The present results provide evidence that some rejuvenation occurred during the process of somatic embryogenesis and resulted in improved shoot growth and rooting of somatic embryo-derived culture compared with “mature” shoot culture. The results reported in this study might be useful in embryogenic systems with low plant conversion rates. The proposed experimental model might also be useful in finding molecular markers of plant ontogeny.

Development of disease-resistant marker-free tomato by R/RS site-specific recombination

The selection marker genes, imparting antibiotic or herbicide resistance, in the final transgenics have been criticized by the public and considered a hindrance in their commercialization. Multi-auto-transformation (MAT) vector system has been one of the strategies to produce marker-free transgenic plants without using selective chemicals and plant growth regulators (PGRs). In the study reported here, isopentenyltransferase (ipt) gene was used as a selection marker and wasabi defensin (WD) gene, isolated from Wasabia japonica as a target gene. WD was cloned from the binary vector, pEKH-WD to an ipt-type MAT vector, pMAT21 by gateway cloning and transferred to Agrobacterium tumefaciens strain EHA105. Infected cotyledons of tomato cv. Reiyo were cultured on PGR- and antibiotic-free MS medium. Adventitious shoots were developed by the explants infected with the pMAT21/wasabi defensin. The same PGR- and antibiotic-free MS medium was used in subcultures of the adventitious shoot lines (ASLs) to produce ipt and normal shoots. Approximately, 6months after infection morphologically normal shoots were produced. Molecular analyses of the developed shoots confirmed the integration of gene of interest (WD) and excision of the selection marker (ipt). Expression of WD was confirmed by Northern blot and Western blot analyses. The marker-free transgenic plants exhibited enhanced resistance against Botrytis cinerea (gray mold), Alternaria solani (early blight), Fusarium oxysporum (Fusarium wilt) and Erysiphe lycopersici (powdery mildew).

Study of geological storage for a candidate CCS demonstration project in Tomakomai, Hokkaido, Japan

The Prime Minister of Japan declared: Japan should positively commit itself to setting a long-term reduction target. For its mid-term goal, Japan will aim to reduce its emissions by 25% by 2020, if compared to the 1990 level, consistent with what the science calls for in order to halt global warming. In order to achieve this target, the government considers CCS (Carbon dioxide Capture and Storage) as an essential method and aims to improve CCS technology to practical levels. Japan CCS Co., Ltd. (JCCS) was established in May, 2008 in order to study the potential and feasibility of CCS demonstration projects in Japan. The company was commissioned by the Ministry of Economy, Trade and Industry (METI) in Japan. Major shareholders of JCCS include electric power companies, upstream to downstream petroleum companies, material companies, engineering companies, etc.. In 2008, an investigation of candidate sites for the CCS demonstration was done over Japan and several promising offshore sites were selected. One of the selected candidate sites is the Tomakomai offshore site in Hokkaido prefecture, located in the northern part of Japan where there are petroleum refineries, paper mills and power plants close to the site. The Yufutsu oil and gas field is located in the eastern part of Tomakomai city where much geological data has been obtained through oil and gas exploration. Basic geological modes were constructed and CO2 reservoir simulation was performed to predict the movement of the stored CO2. Simulation results show us that a sufficient volume of CO2 could be safely stored in the saline aquifers at the Tomakomai site for a demonstration period and afterward. From October to November in 2009, a 3D seismic survey was implemented to confirm the geological potential of the site. Ocean bottom cables enabled us to survey in front of the Tomakomai Port. The survey area (full-stacked area) was 3.0 km (N-S) by 2.7 km (W-E) and about 2 km from the shoreline with several target formations at a depth of about 1,100–3,000 m. This survey was conducted in 4 patches using 2 to 3 sets of ocean bottom cables with the total number of receiver and shot lines being 10 and 26, respectively. Results of this survey were utilized in building the detailed 3D geological model for the CO2 reservoir simulation. Basic plant designing to capture CO2 at the hydrogen process in the petroleum refineries is currently ongoing. The captured CO2 will be injected at 250,000 ton/year for 4–5 years in the demonstration project. Many local organizations, such as the Tomakomai city government and the fisheries cooperative, cooperated to carry out the 3D seismic survey. The Tomakomai government has been strongly willing to invite the CCS demonstration project to the city. By their continuous cooperation is possible to carry forward the feasibility study in order to start the CO2 injection in 3–4 years.

Methodology for dense spatial sampling of multicomponent recording of converted waves in shallow marine environments

A widespread use of converted waves for shallow marine applications is hampered by spatial aliasing and field efficiency. Their short wavelengths require dense spatial sampling which often needs to be achieved by receivers deployed on the seabed. We adopted a new methodology where the dense spatial sampling is achieved in the common-receiver domain by reducing the shot spacing. This is done by shooting one track multiple times and merging the shot lines in an effective manner in a separate processing step. This processing step is essential because positioning errors introduced during the field measurement can become significant in the combined line, particularly when they exceed the distance between two adjacent shot positions. For this processing step, a particular shot line is used as a reference line and relative variations in source and receiver positions in the other shot lines are corrected for using crosscorrelation. The combined shot line can subsequently be regularized for further processing. The methodology is adopted in a field experiment conducted in the Danube River in Hungary. The aim of the seismic experiment was to acquire properly sampled converted-wave data using a multicomponent receiver array. The dense spatial sampling was achieved by sailing one track 14 times. After correcting for the underwater receiver positions using the direct arrival, the crosscorrelation step was applied to merge the different shot lines. The successfully combined result is regularized into a densely sampled data set free of visible spatial aliasing and suitable for converted-wave processing.

P.2.d.003 The omega-3 polyunsaturated fatty acid docosahexaenoic acid suppresses neuroinflammatory responses and induces heme oxygenase-1 expression in BV-2 microglia

Persimmon (Diospyros kaki cv. Jiro) was transformed with the gene encoding the pear fruit (Pyrus communis) polygalacturonase inhibiting protein (PGIP) using Agrobacterium tumefaciens EHA101. Two plasmid constructions were used for transformation; pDU94.0928 containing chimeric genes of PGIP, GUS and NPTII in its T-DNA region, and pYS95.091 containing only the GUS and NPTII sequences (control transformations). Among 165 callus lines obtained from infection by A. tumefaciens with pDU94.0928, 15 showed GUS activity. A PGIP gene fragment of 132 bp was amplified by PCR in nine of 15 callus lines. Shoots were regenerated from three of the nine PGIP-containing callus lines. The transformation of the regenerated shoots was confirmed by PCR and Southern blot analyses using NTPII, GUS and pear PGIP probes. PGIP expression in shoot tissues was analyzed by western blot analysis. The protein expression was observed in all three shoot lines regenerated from transformed calli with the PGIP gene. Fungal PG activity was inhibited by crude extracts from shoots transformed with the PGIP gene substantially more than by those from the control transformants obtained from the transformation with pYS95.091.

Shot lining of magnesium alloy with hard powders

To improve the surface properties, lining of magnesium alloys with hard powders by shot peening was carried out in order. The hard powders were tried to bond to the workpiece surface due to the collision of many shots. In order to fix the hard powders to the surface of the workpiece, the powders were set on an uneven surface. To easily facilitate fixing of powders, lining of the workpiece with the powder sandwiched between two aluminum foil sheets was also attempted. In this experiment, a centrifugal shot peening machine with an electrical heater was employed. The workpieces were magnesium alloys AZ31B and AZ91D, and the hard powders were commercial cemented carbide, alumina, and zirconia. The joinability of hard powders near the lined surface was observed by a optical microscope. The wear resistance was also evaluated by a wear test. The hard powders were successfully bonded to the surface of workpieces by the shot lining process. The results show that the present method is effective in wear resistance of the magnesium alloys.

Virtual source method applied to crosswell and horizontal well geometries

The virtual source method is a useful tool for redatuming a seismic survey below complicated overburden by creating virtual sources at downhole receiver locations, hence generating data independent of the overburden and the time-lapse changes therein. In this article we first apply this technique to crosswell geometries, whereby a virtual crosswell survey is simulated by shooting a line of surface shots passing through two boreholes instrumented with downhole sensors. Using this acquisition geometry, receivers in one of the two wells are turned into virtual sources by correlating the wavefield recorded by those receivers with the recording at receivers in the other well, and summing the correlated data over the surface shots.

Obtaining and Selection of Pancratium Maritimum L. In Vitro Cultures with Acetylcholinesterase Inhibitory Action

ABSTRACTTwo types of plant in vitro systems—calli and shoot cultures were obtained from ovaries of sea daffodil (Pancratium maritimum L.)—a Bulgarian threatened plant belonging to the Amaryllidaceae family. Using TLC method, ten different alkaloids were separated in extracts from in vitro cultures, as well as their abilities to inhibit the acetylcholinesterase (EC. 3.1.1.7) were evaluated by the same techniques. All investigated lines from both type in vitro systems exhibited high levels of somaclonal variability concerning the number and the levels of alkaloids produced. However, the callus cultures produced alkaloids in significantly lower levels compared to the shoot cultures. Two shoot lines (H6L7 and H6L6) producing four alkaloids with high acetylcholinesterase inhibitory action (with Rf values of 0.47, 0.43, 0.25 and 0.12) they were selected as prospective source for further screening of new acetylcholinesterase inhibitors.

ショットライニングと熱処理の複合処理による<br>炭素鋼への鉄-アルミ系金属間化合物皮膜の形成

ショットライニングと熱処理の複合処理による<br>炭素鋼への鉄-アルミ系金属間化合物皮膜の形成

A Direct Simulation Method for Calculating Multiple-hit Vulnerability of Aircraft with Overlapping Components

When components overlap, the aircraft multiple-hit vulnerability analysis usually consists of two steps. The first is to determine the aircraft unique existent states using vulnerable area decomposition method, and the second is to calculate the aircraft cumulated probability of kill using Markov chain method for exact solution or Monte Carlo method for solving the combinatorial explosion problem. This article proposes a direct simulation method for calculating the aircraft multiple-hit vulnerability in order to avoid the complex vulnerable area decomposition issue. In this method, random method is adopted to produce the multiple-hit locations and Bernoulli trial is used to determine the kill or no kill of each component hit by one shot line, and kill tree is checked to determine aircraft existent states during one simulation. When the number of times of simulation is large enough, the aircraft multiple-hit vulnerability can be statistically obtained. Analysis shows that the proposed direct simulation method can provide good accuracy compared with Markov chain method and avoid the combinatorial explosion problem, and does not need the complex vulnerable area decomposition and can directly calculate the aircraft multiple-hit vulnerability. Another important finding is the binomial or Poisson simplified approach is sometimes very poor in accuracy, and should be used cautiously.

Shot Line Geometric Description Method for Aircraft Vulnerability Calculation

This article, firstly, categorizes aircraft's presented area in a particular hit aspect into non-vulnerable region, non-overlapping vulnerable region and overlapping vulnerable region. Then, the aircraft's unique existing states are divided into kill states, intermediate states and no kill states. The parameters pertinent to aircraft's existing states in the above-mentioned regions are analyzed by using the shot line scanning method. Finally, the method provides two kinds of shot line geometric descriptive data: ▪ component's vulnerable area and presented area in each region, which can be used to calculate the component's single-hit vulnerability and ▪ aircraft's unique existing states and the areas corresponding to each state, which can be used to calculate the aircraft's single-hit and multi-hit vulnerability. Examples show that the proposed method can provide the parameters of the aircraft's or its component's area under threat being hit through tracing the shot line path thereby enabling the vulnerability calculated results to conform with reality to larger extent. In addition, the method solves the generality problem in dealing with overlapping components.

Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro: evaluation of multiple nutrient formulations and cytokinin types

The growth of black walnut shoot cultures was compared on media differing in nutrient formulation (MS, DKW, WPM, and 1/2X DKW), cytokinin type (ZEA, BA, and TDZ), and cytokinin concentration. On WPM and 1/2X DKW media, hyperhydricity was observed at frequencies of 60–100% compared with frequencies of 10–40% on the high-salt media (DKW and MS). All three cytokinins facilitated shoot regeneration from nodal cuttings, but recurrent elongation was only observed for BA (5–12.5 μM) and ZEA (5–25 μM) with mean shoot heights of 70–80 mm being possible after two culture periods (6–8 wk) for the fastest elongating lines. ZEA was effective across all six shoot lines with mean shoot heights of at least 35 mm over two culture periods, but two of the shoot lines were ‘nonresponsive’ to BA with mean shoot heights of 50%) axillary proliferation had adverse effects on the morphology and growth potential of the shoots, multiplication strategies based on the use of nodal cuttings are recommended.

A bump on the upper surface of the Philippine Sea plate beneath the Boso Peninsula, Japan inferred from seismic reflection surveys: A possible asperity of the 1703 Genroku earthquake

To reveal a subsurface structure beneath the southern part of the Boso Peninsula, Japan, where the Philippine Sea plate is subducting and great interplate earthquakes associated with the subduction occur repeatedly, we conducted a new seismic reflection survey from March to April 2005 (Boso05). We also reanalyzed old multi-channel seismic (MCS) survey data that had been collected off the Boso Peninsula in 1978 (SK78). We found clear strong reflectors beneath the southern coast of the Boso Peninsula. Since common mid points (CMPs) were distributed widely beneath the study area owing to the design of the receiver and shot lines of Boso05, we selected appropriate directions of stacking lines to get the best image of the dipping reflectors by optimum azimuth search (OAS) processing. We carefully checked the seismic profiles at the intersections of the survey lines to confirm the NNE-dipping configuration of the strong reflectors. These strong reflectors were interpreted as the upper surface of the subducting PHS plate from their locations and the estimated velocities beneath the reflectors. Furthermore, these reflectors revealed a topographic high (bump) beneath the southern coast of the Boso Peninsula where the source fault of the Genroku earthquake of 1703 is thought to be located.