PURPOSE: Current dogma in lifestyle intervention programs emphasize that weight loss secondary to obesity is the primary determinant of phenotypic changes. The present study was designed to examine whether changes in phenotypic outcomes in response to a short-term lifestyle intervention program differ between lean and obese children. METHODS: 16 lean and 17 overweight/obese children (15 boys, 18 girls, age 12.3±0.4 yr, BMI lean (L): 20.5±1.5 vs. obese (O): 34.9±1.9 kg/m2, p<0.0001) were placed on a high-fiber, low-fat diet in the Pritikin Longevity Center 2-week residential program where food was provided ad libitum and daily exercise (2-2.5 hr) was performed. In each subject, anthropometrics and fasting serum samples were acquired pre- and post-intervention for determination of fasting blood glucose, lipids, IL-10, IL-1ra, IL-8, VEGF, PAI-1, and resistin by multiplex assays (Millipore Luminex xMAP). RESULTS: After the 2-wk program, both groups had similar weight loss (L: 3.8% vs. O: 3.4%, p=0.55) but the O group remained overweight/obese (BMI: 33.7±1.8 post vs. 34.9±1.9 kg/m2 pre, waist circumference: 97.0±4.0 vs. 102.4±4.8 cm, p<0.05). RHR and BP (L: SBP: 1.4%, DBP: 6.6%, RHR: 10.2% vs. O: SBP: 8.6%, DBP: 8.2%, RHR: 14.4%, all p>0.10) had comparable significant decreases in both groups, p<0.05. Serum TG (L: 40.0% vs. O: 37.9%, p=0.59), Total-C (L: 24.5% vs. O: 19.3%, p=0.68) and LDL (L: 30.9% vs. O: 22.3%, p=0.64) had similar decreases, all p<0.005. Serum HDL (L: 5.9% vs. O: 0.9%, p=0.27) also decreased significantly, p<0.05. Serum glucose (L: 0.7% vs. O: 4.3%, p=0.36) did not change significantly. Serum IL-8 (L: 33.0% vs. O: 26.6%, p=0.84) decreased significantly in both groups, p<0.05. Serum PAI-1 (L: 29.6% vs. O: 26.0%, p=0.78) and resistin (L: 39.4% vs. O: 35.0%, p=0.68) significantly decreased, p<0.001. VEGF decreased in L (25.5%, p<0.05) but not O (-9.1%, p=0.16). In addition, the anti-inflammatory markers IL-10 and IL-1ra tended to increase in O (57.4%, p=0.14, 27.0%, p=0.21, respectively) and did not change and decreased in L respectively (0.5%, p=0.50 and 37.8%, p<0.05). CONCLUSIONS: These results indicate that intensive lifestyle modification ameliorates numerous metabolic risk factors in both lean and obese children, and even in the face of the obese children remaining obese after the intervention.
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