A4 stainless steel (SS) is a suitable metallic material for heat exchangers due to its inherent resistance to harsh environments and a usually difficult to process due to its high strength and superior plasticity. However, many sophisticated machining strategies exist to compete with the material's responsiveness while generating features in the micro-domain. Out of those, short-pulsed fiber laser (SPFL) is the most eminent choice due to its less arduousness. In this research laser micro-machining process (LMMP) is adopted for producing micro-channels on A4SS with the assistance of SPFL. The designed micro-channel structure holds its applicability in heat exchanger applications. Being a thermal process, the current LMMP may have high machining capability regardless of material properties, but at the same time, it has some adverse effects on machined surfaces. This research attempts to scrutinize the impact of SPFL variables on the channel surface’s characteristics by utilizing different characterization equipment. The SPFL-processed surfaces were investigated as a distinct industrial potential in dry, liquid, active, and inactive gas environments by adjusting the crucial variables of the SPFL. Further, the mechanical property, surface, and sub-surface analysis (including the study of functional groups, phases, morphology, and chemical elements) have also been performed to learn more about the varied environmental implications during LMMP-A4SS interactions.