Lodgepole Pine Research Articles

Functional traits differ across an invasive tree species’ native, introduced, and invasive populations

It is often speculated that non-native invasive species undergo rapid changes in their phenotypic properties (i.e., traits) that provide adaptive advantage in their new environment. However, few studies have directly compared traits of invasive non-native species with their native counterparts to reveal whether such phenotypic changes occur, and which stages of initial introduction and subsequent invasion contribute to these shifts. We studied trait variation of an invasive tree, Pinus contorta, which is native to northwestern North America and invasive in the Patagonia region of South America (i.e., Argentina and Chile). Commercial plantations of P. contorta were introduced extensively in Patagonia from the 1970s onward, from an unknown seed origin within the Pacific Northwest, USA, where three sub-species are found, including subsp. contorta, latifolia, and murrayana. We employed a home-versus-away study approach, where we compared mean growth, defense, and reproduction trait values, and mean within-stand trait variation (Coefficient of Variation, CV) of Patagonia plantations, with the three native sub-species. We further compared mean traits, and trait CVs between invasive P. contorta and the Patagonia plantations from which they escaped. Patagonia plantations shared the most similar mean trait values with subsp. latifolia and murrayana, suggesting possible source populations. However, both mean trait values and trait CVs of Patagonia plantations differed from all three native sub-species, indicating potential founder effects, population bottlenecks, and/or plastic responses to their new environment that occurred during or after introduction. We also found evidence for selective change during invasion; however, these differences did not suggest growth traits were prioritized over defense traits, which was inconsistent with hypotheses that invaders exhibit an evolutionary trade-off between defense traits and growth traits. Our study highlights that processes occurring both at first introduction and establishment, as well as the subsequent invasion phase can influence the phenotype of successful invaders.

Predicting the site productivity of forest tree species using climate niche models

Species occurrence-based climate niche models (CNMs) serve as valuable tools for predicting the future ranges of species’ suitable habitats, aiding the development of climate change adaptation strategies. However, these models do not address an essential aspect - productivity, which holds economic significance for timber production and ecological importance for carbon sequestration and ecosystem services. In this study, we investigated the potential to extend the CNMs to predict species productivity under various climate conditions. Lodgepole pine (Pinus contorta Dougl. ex Loud.) and Douglas-fir (Pseudotsuga menziesii Franco.) were selected as our model species due to their comprehensive range-wide occurrence data and measurement of site productivity. To achieve this, we compared and optimized the performance of four individual modeling algorithms (Random Forest (RF), Maxent, Generalized Boosted Models (GBM), and Generalized Additive Model (GAM)) in reflecting site productivity by evaluating the effect of spatial filtering, and the ratio of presence to absence (p/a ratio) observations. Additionally, we applied a binning process to capture the overarching trend of climatic effects while minimizing the impact of other factors. We observed consistency in optimal performance across both species when using the unfiltered data and a 1:1.5 p/a ratio, which could potentially be extended to other species. Among the modeling algorithms explored, we selected the ensemble model combining RF and Maxent as the final model to predict the range-wide site productivity for both species. The predicted range-wide site productivity was validated with an independent dataset for each species and yielded promising results (R2 above 0.7), affirming our model’s credibility. Our model introduced an innovative approach for predicting species productivity with high accuracy using only species occurrence data, and significantly advanced the application of CNMs. It provided crucial tools and insights for evaluating climate change's impact on productivity and holds a better potential for informed forest management and conservation decisions.

Representation of regeneration dynamics in growth and yield models: a review

Models of forest regeneration dynamics have been less widely applied in forest management than those representing growth and mortality in later stages of stand development, in spite of the critical role of regeneration in maintaining forest ecosystems. This omission is demonstrated by a review of pertinent literature and examined in the context of reforestation in the province of Alberta. Regeneration assessments in Alberta are undertaken before the regeneration phase of stand development is complete. As a result, existing growth and yield models, used to predict whether regeneration performance will meet management objectives, do not adequately represent juvenile mortality, ingress of natural regeneration, stand density, and the responses of ingress and mortality to reforestation treatments. A long-term experiment monitoring regeneration of lodgepole pine stands following harvest has over the last 20 years attempted to address some of the resulting challenges. Opportunities and needs for regeneration modelling include extension to other boreal species and ecotypes, incorporation of climatic variables, and innovations in data collection and analytical techniques. Steps are recommended for expediting the required research. 

Envisioning Transition from Open Landscapes to Forested Landscapes in the Routt National Forest, Colorado, United States

Globally, in remaining wildlands, tree densities and forested cover have increased in grasslands and open forests since European settlement. In the southern Rocky Mountains of Colorado, United States, we determined tree composition and tree cover from historical (years 1875 to 1896) surveys and compared them to current (2002 to 2011) tree composition and current (year 2016) forested land cover for 500,000 ha of the Routt National Forest. Additionally, we examined whether changes in precipitation occurred. Regarding composition, pine (primarily lodgepole pine; Pinus contorta) decreased from 65% to 32% of all trees, with increased subalpine fir (Abies lasiocarpa) from 0.5% to 23% of all trees, and quaking aspen (Populus tremuloides) from 13% to 30% of all trees. According to 80% of 5175 survey points not in forests, the historical landscape was very open, comprised of grasslands, mountain meadows, and other open ecosystems. In contrast, 75% of the current landscape is covered by forests. Change points in the Palmer Modified Drought Index were within historical limits, indicating that forestation was not related to a change in water availability. Based on historical surveys and accounts, we envisioned a historical landscape that was open but embedded with dense lodgepole pine clusters and spruce stands at high elevations, which has now become a predominantly forested landscape of dense forests, similar to global forestation patterns.

Unraveling the multifaceted effects of climatic factors on mountain pine beetle and its interaction with fungal symbionts.

Mountain pine beetles (MPBs) pose a substantial threat to North American pine forests, causing extensive tree mortality over large areas. Their tree-killing ability is closely linked to mass aggregation on host trees triggered via pheromones and dependence on their symbiotic fungi. However, the influence of a changing climate on the biology of MPBs and their co-evolved interactions with their fungal symbionts remains uncertain. To investigate this, male and female pairs of beetles were introduced into freshly cut logs from lodgepole pine trees and placed in controlled climate chambers with manipulated environmental conditions, including two levels of CO2 (ambient vs. 1000 ppm), O3 (ambient vs. 100 ppb) and humidity (33% vs. 65%). The beetle-infested logs were left in these chambers for 1 month and then returned to ambient conditions until brood emergence. Emerging broods were collected for further analysis. Additionally, three species of fungal symbionts (Grosmannia clavigera, Ophiostoma montium and Leptographium longiclavatum) were subjected to the same CO2 , O3 and humidity conditions for 5 days. Lower humidity promoted MPB reproduction and fungal growth. Elevated CO2 accelerated larval growth and emergence while improving brood pheromone production. Elevated O3 had a negative impact on MPB reproduction and brood fitness while improving its immune responses to an entomopathogenic fungus (Beauveria bassiana). It also inhibited fungal growth and reproduction, whereas elevated CO2 had varied (positive or negative) effects on fungal growth and ergosterol (proxy to fungal mass) production depending on the fungal species. Together, these findings suggest that climate change can potentially alter the interactions between MPBs and their fungal symbionts, highlighting the importance of understanding how climate change affects forest pests and their symbiotic relationships to develop effective management strategies in the future.

Variations in gaseous and particulate emissions from flaming and smoldering combustion of Douglas fir and lodgepole pine under different fuel moisture conditions

The effects of fuel moisture content (FMC) on emissions under both flaming and smoldering combustion conditions were studied for two different vegetative fuels, Douglas fir and lodgepole pine. A custom linear tube-heater apparatus was developed to produce steady-state emissions for two extreme FMC, recently live (FMC ∼ 50%) and fully dead and dry (FMC ∼ 3%). The concentrations of particulate matter and gaseous species measured using a TSI DustTrak and Fourier transform infrared spectroscopy (FTIR), respectively, showed a significant relationship with FMC for both flaming and smoldering combustion conditions. As expected, smoldering combustion showed larger production of CO, particulate matter, and unburned hydrocarbons when compared to flaming combustion. Unburnt hydrocarbons with higher emission factors produced during smoldering are CH4, C2H2, C2H6, C4H10, C4H8, C2H4, C3H6, CH2O2, C3H4O, CH2O, and CH3OH. This work also identified butane (C4H10) and hydrogen bromide (HBr) as gaseous species, which have not been commonly reported in literature. Acrolein (C3H4O) was also identified during combustion and it has previously reported only by ion detection method. These emissions were further investigated by performing a carbon balance of the carbon in the fuel vs. emitted carbon, and these were compared to literature values. Flaming-mode emission factors significantly correlated with FMC, while FMC had little influence on emission factors for smoldering combustion, when considering the dry mass of the burned fuel. These variations were not the same for each fuel species, suggesting that the type of fuel plays an important role in burning behavior and emissions, possibly due to the chemical makeup of moist and recently live fuels. The results were discussed and compared with previous measurements, where good agreement was observed. This study provides an important emissions dataset for modeling emissions from common wildland fuels under varying FMC and combustion conditions.Novelty and significanceThis research investigates the impact of fuel moisture content (FMC) on emissions during flaming and smoldering combustion of Douglas fir and lodgepole pine. A unique linear tube-heater apparatus was developed to maintain steady-state emissions for extreme FMC levels of recently live (FMC 50%) and fully dead/dry (FMC 3%). The study reveals a noteworthy correlation between FMC and concentrations of particulate matter and gaseous species. This finding provides valuable insights into combustion behavior. It also identifies butane (C4H10), acrolein (C3H4O), and hydrogen bromide (HBr), not commonly reported in literature. Additionally, the study highlights the varying influence of FMC on emission factors for flaming and smoldering combustion, indicating the pivotal role of fuel type in emissions. The dataset obtained serves as a valuable resource for modeling emissions from wildland fuels under diverse FMC and combustion conditions, advancing our understanding of fire behavior and environmental impacts.

Iterative nearest neighbour age-height curve adjustment: Addressing the impact of spatial heterogeneity on longitudinal forestry provenance trial data

Spatial heterogeneity in long term forestry genetics trials can obscure genetic variation and influence conclusions made based on the data. Nearest neighbour adjustment methods have been employed to account for spatial patterns, however such methods are limited in their applicability to longitudinal provenance trial data because of the need to account for repeated measures as well as the interest in preserving among-site variation. In this study, a novel approach combining nearest neighbour adjustment techniques with longitudinal data analysis concepts was developed to adjust lodgepole pine (Pinus contorta var. latifola Douglas) provenance trial data for spatial heterogeneity. Individual height-age logistic growth curves were fit to each tree to age 35, and the horizontal asymptote parameters of the height-age curves were adjusted for spatial patterns using iterative nearest neighbour adjustments. The methods developed in this study successfully removed positive spatial correlation and reduced the interaction between block and provenance, thus reducing changes in population ranking among blocks within sites. The adjustments applied caused large shifts in population ranks at many sites, indicating that seed selection decisions based on data not adjusted for spatial heterogeneity could lead to selecting non-optimal populations for a given site. This research provides a methodological framework for adjusting longitudinal genetics trial data for underlying site conditions and spatial patterns. Additionally, a spatially adjusted version of a large and extensively utilized provenance trial dataset was created for use in future analyses.

Evapotranspiration and its partitioning during and following a mountain pine beetle infestation of a lodgepole pine stand in the interior of British Columbia, Canada

IntroductionMassive tree mortality events in western Canada due to widespread infestation by mountain pine beetle (MPB) are expected to impact local-to-regional evapotranspiration (ET) dynamics during and after a disturbance. How ecosystem-level ET and its components may vary with canopy-tree mortality (treefall) and subsequent understory recovery remains unclear.MethodsWe used 10 years of continuous eddy-covariance and remote-sensing data (2007–2016) and machine-learning models based on random forest and xgboost to determine forest- and climate-driven effects at temporal scales appropriate for a lodgepole pine-dominated stand following a major, five-year MPB disturbance initiated in the summer of 2006.ResultsTotal annual ET over the 10 years ranged from 207.2 to 384.6 mm, with annual plant transpiration (T) contributing to 57 ± 5.4% (mean ± standard deviation) of annual ET. Annual ET initially declined (2007–2011) and then increased (2011–2016), with ET and T/ET increasing at statistically non-significant rates of approximately 3.2 and 1.2% per year from 2007 to 2016. Air temperature (Ta) and vapor pressure deficit (VPD) were the most important predictors of seasonal variation in ET and T/ET during the 10-year period, with high Ta, VPD, and photosynthetically active radiation (PAR) causing ET and T/ET to increase. Annual ET increased with both increasing spring Ta and decreasing VPD. Annual T/ET was shown to increase with increasing VPD and decrease with increasing volumetric soil water content at a 5-cm depth (VWC5). Enhanced vegetation index (EVI, an indicator of canopy greenness) lagged T and overstory tree mortality, whereas previous- and current-year values of EVI were shown to be poor predictors of annual ET and T/ET.Discussion and conclusionsThese findings suggest that the promotion of climate factors on forest ecosystem-level water vapor fluxes may offset reductions promoted by MPB outbreaks. Climate processes affected water vapor fluxes more than biotic factors, like stand greenness, highlighting the need to include climate-regulatory mechanisms in predictive models of ET dynamics during and subsequent to stand disturbance. Climate and forest-greenness effects on water vapor fluxes need to be explored at even longer time scales, e.g., at decadal scales, to capture long-drawn-out trends associated with stand disturbance and its subsequent recovery.

Evidence that Ophiostomatoid Fungal Symbionts of Mountain Pine Beetle Do Not Play a Role in Overcoming Lodgepole Pine Defenses During Mass Attack.

Mountain pine beetle (MPB; Dendroctonus ponderosae Hopkins) is a devastating forest insect pest that has killed millions of hectares of pines in western North America over the past two decades. Like other bark beetles, MPB vectors ophiostomatoid fungal species, some of which are pathogenic to host pine species. The phytopathogenicity of these fungal symbionts has sparked considerable debate regarding their role in facilitating MPB attack success. We tested the hypothesis that MPB ophiostomatoid fungal associates like Grosmannia clavigera (Robinson-Jeffrey and Davidson) Zipfel, de Beer and Wingfield contribute to overwhelming host defenses during MPB mass attack. We compared responses of mature lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia Engelm.) trees growing in natural stands that were mass attacked by MPB with those inoculated with G. clavigera by examining host defense hormones, secondary metabolites, and gene expression profiles. The jasmonate and ethylene signatures of necrotrophic pathogen-triggered response were identified in G. clavigera-inoculated trees, but only the jasmonate signature of a herbivore-triggered response was measured in MPB-attacked trees. Several G. clavigera-induced changes in pine phenolic metabolite profiles and phenolic biosynthesis gene expression patterns were absent in MPB-attacked pines. These findings indicate that ophiostomatoid fungi like G. clavigera are not a major factor in overwhelming host defenses during MPB mass attack. Instead, fungal pathogenicity likely is more important in aiding MPB colonization and development within the host tree. Phenolics appear to play a larger role in the host response to G. clavigera than to MPB, although phenolics may also influence MPB feeding and behavior. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.

Association of Onnia subtriquetra with living and dead bishop pine (Pinus muricata) and shore pine (Pinus contorta var. contorta) in California, USA

AbstractWe report observations of Onnia subtriquetra on bishop pine (Pinus muricata) and shore pine (Pinus contorta var. contorta) from north coastal California. Our identification of this fungus is supported by molecular information, morphological characteristics, and and a description of the observed range of the fungus on the West Coast. These observations represent an expansion of the geographic and host ranges of Onnia subtriquetra, which on the observed sites is generally associated with declining tree condition and the presence of other native forest pathogens and insects.

Growth response to pre-commercial thinning of lodgepole pine is short-term but the effects on size distribution persist for decades

Pre-commercial thinning in Alberta is a potential option for increasing the growth rate and shortening the rotation age of regenerating forests. Previous studies have focused on the evaluation of either the immediate- or long-term response to thinning after many decades. Our current study compares the pre-commercial thinning response in lodgepole pine for a 10-year period immediately after thinning, and again 38-45 years after thinning, based on 22 paired plots of precommercially thinned trials in Alberta. The first group of plots was pre-commercially thinned in 1984, measured in 1985 and 1995 (short-term) and the second group was pre-commercially thinned between 1962 and 1969 and measured in 2007 and 2017 (long-term). In the short-term, individual tree DBH growth was 56% greater in pre-commercially thinned plots, while in the long-term plots, there were no measured growth differences between pre-commercially thinned and unthinned plots. Small- and medium-sized trees benefited more from pre-commercial thinning than larger trees. However, at the stand level, the number and volume of merchantable-sized trees (≥13.5 cm DBH) were higher in pre-commercially thinned plots than in unthinned plots in both the short- and long-term. Although the growth response of thinning appeared to be a short-term response (number and volume of larger trees), yield at the end of the long-term measurement period was still higher in pre-commercially thinned than in unthinned plots.

Influence of irregular shelterwood treatments on intensity and severity after a large wildfire in lodgepole pine stands: A case study from the interior British Columbia.

Climate change has significantly impacted the wildfire regimes in lodgepole pine forests, resulting in prolonged fire seasons and altered fire behaviour. In North America, fire patterns have shifted towards more frequent and severe wildfires after a century of fire suppression. In response, silviculture practices in fire-prone areas should aim to restore diverse forest structures that are resistant or resilient to wildfires. In Western Canada, where forestry is a key industry, interest in seeking silvicultural solutions for promoting forest resilience to wildfires has increased following the devastating wildfire seasons between 2017 and 2023. Irregular shelterwood, a silvicultural system with a relatively short history of implementation in British Columbia, has been deployed in ecologically sensitive areas to promote structural heterogeneity and meet management goals for biodiversity and wildlife values. Although the impacts of irregular shelterwood on wildlife habitat and abundance have been well studied, the interaction between wildfire and the stand structure created by irregular shelterwood remains poorly understood. To understand the effectiveness of the irregular shelterwood in building wildfire resilience, we present a study of a lodgepole pine stand that was treated with irregular shelterwood and partially burned in a wildfire in 2017. This study collected ground fuel, canopy fuel, and tree data from four stand types (irregular shelterwood treated-burnt, treated-unburnt, untreated-burnt, and untreated-unburnt) and analyzed the difference in char height and fire-induced mortality between burnt and unburnt conditions, with irregular shelterwood treatment being a variable. The results demonstrated reduced wildfire effect in the irregular shelterwood stand in this region of British Columbia. This observation was made at a stage where the openings have not been colonized by regeneration. This case study provides valuable insights into the effectiveness of irregular shelterwood in mitigating wildfire risk, and proposes a potential silviculture solution to promote forest resilience to wildfire.

Influence of mountain pine beetle outbreaks on large fires in British Columbia

AbstractA key uncertainty in understanding climate change effects on wildfires in western North America is the role of mountain pine beetle (MPB) outbreaks in driving wildfire occurrence and severity. In this study, we investigated the complex relationship between MPB outbreaks, other environmental factors, and wildfire occurrence in British Columbia (BC), Canada. We adopted a fire risk analysis method developed for fire occurrence prediction to separate the effect of changing fuel conditions on wildfires in BC when neither post‐outbreak fuel conditions, climate, nor management is stationary. Using lasso‐logistic regression and a novel variable ranking procedure, we determined that MPB‐affected areas had 1.7 times more large lightning‐caused fires (≥100 ha), as the likelihood of large lightning‐caused fires increased by 40% in these areas and likely contributed to the increased burned areas in BC. Meanwhile, the likelihood of large human‐caused fires decreased in MPB‐affected areas. Fire weather factors were most influential for both lightning‐ and human‐caused fires, while anthropogenic factors were most influential for human‐caused fires. Fuel dynamics following MPB outbreaks vary across the wide distribution of a host species such as lodgepole pine, at stand and landscape levels. Furthermore, the expression of the effects of MPB and other disturbances on wildfire is also conditional on, as well as confounded with, many other environmental factors and management activities that vary across western North America. Therefore, a lack of consensus on the impacts of MPB on wildfire is not surprising.

Peculiarities of determining the vital state of lodgepole Scots Pine by electrophysiological methods

Peculiarities of determining the vital state of lodgepole Scots Pine by electrophysiological methods

The effect of forked trees on harvester time consumption in a Pinus contorta final-felling stand

The effect of forked trees on harvester time consumption in a Pinus contorta final-felling stand

Quantification of heritable variation in multiple lodgepole pine chemical and physical traits that contribute to defense against mountain pine beetle (Dendroctonus ponderosae)

Bark beetles are major causes of conifer mortality worldwide. We quantified genetic contributions to key chemical and physical traits in lodgepole pine that contribute to defense against mountain pine beetle, a native tree-killing herbivore. Assays were conducted with mature trees in replicated provenance trials. Among chemical defenses, monoterpene composition (relative proportions of various compounds) had higher genetic variability than monoterpene concentration (absolute quantities). Additionally, constitutive concentrations had higher genetic variabilities than induced concentrations elicited by simulated attack. Resin flow elicited by mechanical wounding likewise showed very low genetic variability. In previous studies with this and related systems, induced monoterpene concentration and resin flow are the two best predictors respectively of which trees will survive outbreaks under natural conditions. This means that expression of the most efficacious defense traits is strongly influenced by environmental factors. These low heritabilities despite their survival values likely arise from specific life history and ecological aspects of this system, such as the lengthy period between when lodgepole pines begin cone production and become large enough for brood development, the extended viability of serotinous cones post-mortem, the high heterogeneity of both resources and stressors that trees experience across landscapes, and a strong proximate-neighbor effect on tree mortality arising from beetle aggregation pheromones. However, low heritability values do not preclude long-term evolvability and coadaptation of defenses, for which there is extensive mechanistic and associational evidence in this system. Among other traits we evaluated, sesquiterpenes had higher genetic variability than monoterpenes. Sesquiterpenes are not known to influence conifer-bark beetle interactions but have anti-herbivore properties in some other plant-insect systems. The lengths of lesions formed in response to simulated attack showed no heritable component and likewise do not predict tree survival in nature. Bark texture was highly heritable and is known to influence beetle post-landing orientation and colonization patterns, but whether it influences survival patterns at the whole-tree level is unknown. Relationships between tree defenses and growth were generally positive, suggesting that environmental heterogeneity in resource quality among trees supersedes potential underlying substrate allocation processes within trees. Similarly, relationships between various defense traits were generally positive rather than showing trade-offs, suggesting conifer resistance to native bark beetles entails coordinated defense syndromes. Implications to tree breeding, forest management, and coevolution between long-lived plants and rapidly reproducing herbivores are discussed. The extent to which these trends may apply to other bark beetle systems is evaluated, along with specific priorities for future research.

Can the Seed Trade Provide a Potential Pathway for the Global Distribution of Foliar Pathogens? An Investigation into the Use of Heat Treatments to Reduce Risk of Dothistroma septosporum Transmission via Seed Stock.

The international plant trade results in the accidental movement of invasive pests and pathogens, and has contributed significantly to recent range expansion of pathogens including Dothistroma septosporum. Seeds are usually thought to present a lower biosecurity risk than plants, but the importation of Pinus contorta seeds from North America to Britain in the mid-1900s, and similarities between British and Canadian D. septosporum populations suggests seeds could be a pathway. Dothistroma septosporum has not been isolated from seeds, but inadequately cleaned seed material could contain infected needle fragments. This case study investigated whether cone kilning, and wet and dry heat treatments could reduce D. septosporum transmission without damaging seed viability. Pinus needles infected with D. septosporum were incubated alongside cones undergoing three commercial seed extraction processes. Additional needles were exposed to temperatures ranging from 10 to 67 °C dry heat for up to 48 h, or incubated in water heated to between 20 and 60 °C for up to one hour. Pinus sylvestris seeds were exposed to 60 and 65 dry heat °C for 48 h, and further seed samples incubated in water heated to between 20 and 60 °C for up to one hour. Dothistroma septosporum survived the three kilning processes and while seeds were not damaged by dry heat exceeding 63.5 °C, at this temperature no D. septosporum survived. Wet heat treatments resulted in less than 10% pathogen survival following incubation at 40 °C, while at this temperature the seeds suffered no significant impacts, even when submerged for one hour. Thus, commercial seed kilning could allow D. septosporum transmission, but elevated wet and dry heat treatments could be applied to seed stock to minimise pathogen risk without significantly damaging seed viability.

Transferability of a Mask R–CNN model for the delineation and classification of two species of regenerating tree crowns to untrained sites

Following harvest, monitoring reforestation success is a crucial component of sustainable management. In Alberta, Canada, like other jurisdictions, the efficiency of the current plot-based forest regeneration monitoring regime is challenged by the cost of accessibility and the declining availability of qualified field crews. Fine spatial resolution imagery and deep learning have been proposed as alternative monitoring tools and have proven successful under experimental conditions, yet how successfully models can be applied and transferred between a range of untrained sites and conditions remains unclear.In this research, we repurposed a mask region-based convolutional neural network (Mask R–CNN) model that was previously trained to delineate coniferous tree crowns to instead segment instances of two species of regenerating conifers. We transferred learned parameters by replacing original single-class labels with photo-interpreted species information and retraining a selection of the network's parameters. We assessed the transferability of the new model by testing on five untrained sites, representing a range of forest types and densities typical of reforestation in the region. Results yielded a mean average precision (mAP) of 72% and average class F1 scores of 69% and 78% for lodgepole pine (Pinus contorta) and white spruce (Picea glauca), respectively, demonstrating successful transferability. We then investigated an additional transfer learning scenario by iteratively adding data from four of the five sites to the training set while reserving data from the remaining site for testing. On average, this improved mAP by 5%, lodgepole pine F1 by 7%, and white spruce F1 by 3%, and demonstrated that trained models can be continuously improved as sufficiently representative data becomes available.

Clark's nutcracker forest community visitation: Whitebark pine maintains a keystone seed disperser.

Clark's nutcrackers (Nucifraga columbiana) are obligate seed dispersers for whitebark pine (Pinus albicaulis), but they frequently use other conifer seed resources because of annual variability in cone production or geographic variation in whitebark pine availability. Whitebark pine is declining from several threats including white pine blister rust, leading to potential population declines in the nutcracker and the pine. We hypothesize that where there are few additional seed resources, whitebark pine becomes the key and limiting resource supporting nutcracker populations. We investigated how nutcrackers use coniferous forest community types within Yellowstone National Park to determine potential seed resources and the importance of whitebark pine. We established sites representing five forest community types, including whitebark pine, lodgepole pine (P. contorta), Engelmann spruce (Picea engelmannii), limber pine (P. flexilis), and Douglas-fir (Pseudotsuga menziesii). Each transect annually generated nutcracker point counts, conifer cone production indices, community composition data, and seed resource use observations. We compared hierarchical distance sampling models, estimating nutcracker density and its relationship to forest community type, seed harvesting time-period, year, study site, and cone seed energy. We found cone production varied across years indicating annual variability in energy availability. Nutcracker density was best predicted by forest community type and survey time-period and was highest in whitebark pine stands during the mid-harvesting season. Nutcracker density was comparatively low for all other forest community types. This finding underscores the importance of whitebark pine as a key seed resource for Clark's nutcracker in Yellowstone National Park. The decline of whitebark pine potentially leads to a downward spiral in nutcrackers and whitebark pine, arguing for continued monitoring of nutcrackers and implementation of restoration treatments for whitebark pine.

Distribution of Cronartium x flexili, an interspecific hybrid of two fungal tree rust pathogens, in subalpine forest ecosystems of western USA

Interspecific hybridization plays a key role in the evolution of novel fungal pathogens, and when it occurs between native and invasive species, can lead to potentially serious consequences. In this study, we examined the temporal and spatial distribution of a recently detected hybrid (Cronartium x flexili) of two tree pathogens, invasive to North America Cronartium ribicola and native Cronartium comandrae. In total, 726 and 1452 aecia from 178 Pinus contorta ssp. latifolia and 357 Pinus flexilis trees were collected from 26 sites in four national forests in 2019–2021. Using morphological and molecular analyses, 71 aecia collected from 25 P. flexilis trees had intermediate morphology and contained heterozygous SNPs in two genomic regions. Population analyses revealed the presence of multiple hybrid genotypes randomly distributed among sites and years. No aecia from P. contorta ssp. latifolia were identified as hybrids suggesting unidirectional gene flow from native C. comandrae to invasive C. ribicola. Aeciospores from 2 hybrid aecia produced urediniospores on Ribes nigrum. Overall, these results suggest that, even though low in frequency, C. x flexili is persistent in the region and has pathogenic potential. Hybrid expansion into the large range of susceptible pines could have cascading impacts on forest health.