Shimadzu UV-1800 Research Articles

Visible-Light-Driven Methane Conversion with Oxygen Enabled by Atomically Precise Nickel Catalyst

It remains an extreme challenge to activate thermodynamically unfavorable, chemically inert methane molecules under mild conditions. Herein, we report a molecular-like nickel-thiolate hexameric clu...

Hubungan Sifat Optik terhadap Performa Kinerja Sel Surya DSSC Transparan Berbahan Dye DN-F01 sebagai Sensitizer

<p>Dye sensitized solar cell (DSSC) is the third generation of solar cells that currently developing by researchers to get the best performance. The selection of dye as a component of DSSC has a big influence in the light absorption process. In this study, DN-F01 organic dye was used as a sensitizer with various concentrations. The optical properties test was carried out on the DN-F01 dye solution by UV-Vis spectrophotometer (Shimadzu UV-1800) and the DSSC fabricated performance was tested by portable solar simulator (ORIEL Sol1A). From the results, the DN-F01 absorbance has ability to absorb light in the 300-500 nm wavelength range. The greatest efficiency value in this research is 2.44% obtained from the sample was immersed with the largest concentration of dye DN-F01 which has the smallest band gap energy of 2.38 eV.</p>

Analytical Methods for Simultaneous Estimation of SGLT2 Inhibitor and DPP-4 Inhibitor in their Combination for Treatment of Type 2 Diabetes Mellitus

The Saxagliptin Hydrochloride (SAXA) and Dapagliflozin (DAPA) Fixed-Dose Combination has recently been approved for the treatment of Mellitus type 2 diabetes. In order to simultaneously estimate SAXA and DAPA is a bulk product and its formulation, the study aimed at developing a simple, fast, sensitive, and validated UV-Spectrophotometric and reversed-phase high-performance liquid chromatography (RP-HPLC) methods. Simultaneous equation UV method was performed on Shimadzu UV-1800 Spectrophotometer based on measurement of SAXA and DAPA absorption in methanol at 210 nm and 224 nm, respectively, over 6-22 μg / mL and 12-44 μg / mL linear ranges. RP-HPLC method was designed using an HPLC system-equipped PDA detector. The method has been validated for SAXA and DAPA for a range of 8 to 22 μg / ml and from 16 to 44 μg / mL. In compliance with ICH guideline Q2(R1), the optimal approach is successfully validated. The results showed that the method was accurate (98.22–100.28 percent w / w and 99.48–100.15 percent w / w SAXA and DAPA, respectively) and precise (percentage of relative standard deviation < 2.0). Developed methods follow ICH Q2 (R1) criteria and sufficient to apply regulatory versatility for submission.

Development of Formulation and Technology of Low-lactose Dairy Beverage Made from Goat Milk

The concept of lactose intolerance and solution approaches of this problem using modern dairy processing technologies are considered in this study. The protein and lipid constituents were calculated to select the basis of a low-lactose milk beverage. The β-galactosidase preparation was chosen and enzymatic hydrolysis conditions were optimized to obtain a dairy drink that meets the requirements for low-lactose products. To determine the residual amount of lactose in fermented milk, the method for determining whole blood glucose by enzymatic colorimetric method using Shimadzu UV-1800 spectrophotometry was adopted. The influence of technological factors on the dairy low-lactose drink properties was studied. The formulation has been developed and the manufacture process for obtaining a range of low-lactose beverages was adopted, the protein and lipid constituents in the finished product were calculated. The results of this study are considered to be socially significant since the low-lactose products are intended for people suffering from lactase deficiency.

Transovarial effect of the mixture of metal nanoparticles (Ag, Cu, Fe, MnO2) on biochemical indices of blood of one-day-old chickens compared to metal salts

Aim. Due to small sizes of metal nanoparticles (NPMe), after getting into the organism, they penetrate different internal organs, overcoming hematoencephalitic and placental barriers, and may cause different pathophysiological effects in the organism. Thus, the aim of our study was to study transovarial effect of NPMe mixture (Ag, Cu, Fe, MnO2 ) on biochemical indices of blood of one-day-old chickens compared to salts of relevant metals. Methods. The studies were conducted using Hisex White cross chickens (n = 28) with the sex ratio of roosters to hens of 1:6, which were fed for 37 days with the following additives: an experimental sample of NPМе mixture, containing nanoparticles of silver (31.5 ± 0.9 nm), iron (100.0 ± 10.0 nm), copper (70.0 ± 4.0 nm) and manganese peroxidase (50.0 ± 3.0 nm), in the doses of 0.3 and 4.0 mg/kg of bodyweight (experiments ІІ and ІІІ); solution of salt mixture – AgNO3 , (CuSO4 ∙ 5H2 O), (MnSO4 ∙ 5H2 O) and (FeSO4 ∙ 7H2 O) (experiment І); distilled water (control). Starting with Day 30 of the experiment, eggs were collected from poultry in each group for a week and placed for further incubation. Blood samples were taken from hatched one-day-old chickens (n = 93) for biochemical studies. The registration of biochemical indices was conducted using spectrophotometer SHIMADZU UV-1800 (Japan). Results. The experiments determined the mechanism of toxic effect of NPMe mixture after its administration to chickens in the dose of 4.0 mg/kg of bodyweight, which was manifested in decreasing the natural resistance (tendency to the increase in seromucoids – immunosuppressive proteins), excessive formation of toxic products of purine exchange (increase in the concentration of uric acid by 77.6 %; P < 0.05) and straining of enzyme systems of natural detoxication (increase in the activity of alanine aminotransferase and aspartate aminotransferase by 23.8 and 31.6 %; P < 0.05) with the development of oxidative stress (increase in the content of diene conjugates and malondialdehyde by 19.3 and 23.8 %; P < 0.01) in the organism of one-day-old chickens. The mechanism of pharmacological effect of NPMe mixture at its administration to chickens in the dose of 0.3 mg/kg of bodyweight lies in enhancing immune reactions (increasing the amount of general proteins and globulins by 14.4 and 12.0 %; P < 0.05) along with the antioxidant effect via the release of vitamins A and E from the endogenous depot (by 14.7 and 25.7 % above the control; P < 0.05) in the organism of one-day-old chickens. Conclusions. In conditions of transovarial transmission, there is confi rmed advantage of the effect of NPMe mixture (Ag, Cu, Fe, MnO2 ) in the dose of 0.3 mg/kg of bodyweight of laying hens comparing to the effect of the analogue in the form of the mixture of metal salts, which may help in achieving a high percentage of liveability of young birds, forming defensive reactions in the organism of chickens and ensuring full realization of genetic potential in terms of meat or egg productivity of agricultural poultry. The mechanism of toxic effect of metal nanoparticles in the dose of 4.0 mg/kg of bodyweight was determined which will allow elaborating safe regulations for the application of non-organic nanomaterials in modern poultry breeding and timely management of the risks of their application.

Spectrophotometric Quantification of Anti-inflammatory Drugs by Application of Chromogenic Reagents.

Simple, specific, accurate, precise, sensitive, and cost effective spectrophotometric methods were developed and validated for quantification of the drugs lornoxicam (LOR) and mesalamine (MES) in pure form and in pharmaceutical formulations. A Shimadzu UV-1800 double-beam UV-Visible spectrophotometer having a spectral bandwidth of 0.1 nm with wavelength accuracy ±0.1 nm with a pair of 1 cm path length matched quartz cells was used to measure the absorbance of the resulting solution. Method I was used for the quantification of LOR, based on measurement of the absorbance of bluish green chromogen complex at 760 nm, which is formed by the reaction of LOR with ferric chloride and potassium ferricyanide (redox technique). Method II was used for the quantification of MES, based on measurement of the absorbance of yellow chromogen at 400 nm, which is formed by the condensation reaction of the primary amino group of MES with salicylaldehyde reagent (SA) (Schiff base formation). Both methods obeyed Beer's law in the concentration range of 0.5-4.5 μg/mL and 0.2-1.7 μg/mL with good correlation coefficients of 0.9974 and 0.998 for methods I and II, respectively. The developed method is simple, sensitive, and specific, which was validated statistically as per ICH guidelines, and can be used in the routine analysis of LOR and MES in pharmaceutical dosage forms.

Validation of a Thiocyanate Method on the Shimadzu UV-1800 Spectrophotometer

Abstract Objectives Sodium nitroprusside is used in treatment of hypertensive emergency. In the body, nitroprusside is metabolized to cyanide and cyanide to thiocyanate. Thiocyanate concentration is used to monitor nitroprusside therapy and to evaluate possible exposure to cyanide. A method validation study was performed to assess analytical performance of a new spectrophotometry-based laboratory-developed test for serum thiocyanate. Methods Serum proteins were precipitated using trichloroacetic acid and ferric nitrate. The resulting red-colored solution was measured spectrophotometrically at 450 nm using the Shimadzu UV-1800 spectrophotometer (Shimadzu Scientific Instruments, Columbia, MD). Method precision, linearity, method comparison, and effect of interferences were evaluated according to Clinical Laboratory and Standards Institute guidelines. The Microsoft Excel Analyze-It application was used for statistical analysis. Results Between-run coefficients of variation (CVs) for quality control materials at thiocyanate concentrations of 5 mg/L, 20 mg/L, and 50 mg/L were 9.64%, 6.26%, and 4.25%, respectively. The assay was linear over the analytical measuring range of 5 mg/L to 200 mg/L, with an r2 value of 0.9982. The Deming regression equation for the UV-1601 (comparator) and UV-1800 (new) method was: UV-1800 = 0.998 (95% CI, 0.9762-1.001) UV-1601 + 0.79 (95% CI, –0.1379 to 1.733), and the standard error of the regression was 1.8 mg/L. Interference of <7% was observed in the presence of hemolysis, icterus, and lipemia. Conclusion The analytical performance of the new Shimadzu UV-1800 spectrophotometer method is adequate for measurement of serum thiocyanate in patients on nitroprusside therapy or with cyanide poisoning.

Purification of acidic highly mineralized spent regeneration solution of Novo-Salavatskaya TTP

AnnotationThe results of laboratory studies and industrial experiments on the neutralization and purification of highly mineralized acid waste regeneration solutions of the ion-exchange water-preparation plant H-cationite filters are given. As the reagent the carbonate sludge from the pre-cleaning of the water treatment plant was used. A sludge was used in a dry dehydrated form. During a laboratory study, it was shown that there are a significant sulfate content reduction and a pH increase. The sulfate determination was carried out by turbidimetric method using a Shimadzu UV-1800 spectrophotometer. The water treatment sludge using at Novo-Salavatskaya TPP is able to reduce the sulfate content in acid waste water from 16.8 g / l to l g / l. This way of neutralizing can reduce these penalty sanctions for thermal power plants for wastewater discharge.

Comparison Between Surfactant and Chitosan Organo-kaolin Adsorbents to Remove Metal and Organic Compound in Peat Water

This study compares adsorbents to remove metal and organic compounds in peat water solution. The kaolin adsorbent was modified with organic compounds (organo-kaolin). Modifications used two types of organic compound namely surfactant and chitosan with the organic compound concentration used of 15%, 39% and 45% respectively from the total weight of 200 grams adsorbent. The contact time used was 30 minutes with stirring speed of 90 rpm. The concentration of metal ion Fe and organic compound was analyzed by Atomic Absorption Spectrometer (AAS) (Shimadzu UV-1800) and Total Organic Carbon (TOC), the determination of the characteristics for wavelengths before and after treatment used Spectrophotometer Fourier Transform Infra-Red (FTIR) IR Prestige-2, while organo-kaolin surface morphology used SEM. The results of the study show that organo-kaolin surfactant and chitosan successfully reduces Fe metal ion in water up to 91.8% and 88.2% of the initial concentration of 2.07 mg/L from peat water. Both organo-kaolin modification (surfactant and chitosan) ability to remove organic compound was 46.7% and 35.7%. The results of FTIR spectra testing and SEM test also show differences before and after the absorption of the metal ion Fe and organic compounds.

SPATIAL DISTRIBUTIONS OF NUTRIENTS AND CHLOROPHYLL-A: A POSSIBLE OCCURRENCE OF PHOSPHORUS AS A EUTROPHICATION DETERMINANT OF THE JAKARTA BAY

Phytoplankton blooms due to nutrient enrichment (eutrophication) have been considered as the primary factor causing several massive fish kills occurred in the Jakarta Bay. This study aims to determine the spatial variation of phytoplankton abundance based on chlorophyll-a concentration and its relationship with nutrient level and composition. A field observation was conducted in July 2015 measuring chlorophyll-a and nutrient concentrations. Chlorophyll-a was measured using fluorometer Trilogy AU-10 while nutrients was using spectrophotometer Shimadzu UV-1800. The result showed that chlorophyll-a concentration was relatively higher in the western compared to the eastern region of the Jakarta Bay. In addition, the western region was high in orthophosphate, nitrate and silicate while the eastern region was high in ammonium and nitrate. Statistical analysis shows that chlorophyll-a had the highest significant correlation against orthophosphate in the western region and chlorophyll-a had no significant correlation with nutrients in the eastern region. It seems that phosphorus was the primary determinant of chlorophyll-a variability with a concentration of orthophosphate of more than 0.028 mg P/L is required to increase chlorophyll-a concentration. In addition, low orthophosphate concentration (0.014 mg P/L) below its minimum requirement for maximum phytoplankton growth was likely the factor causing the absence of chlorophyll-a and nutrients relationship in the eastern region. In summary, phosphorus is seemingly the primary eutrophication determinant in the Jakarta Bay.

Optical properties of dye DN-F01 as sensitizer

<p class="Abstract">Dye-Sensitized Solar Cell (DSSC) is a solar cell made from organic material that works with an electrochemical photo system to convert solar energy into electrical energy. The components of DSSC consist of transparent conductive substrates, dyes, semiconductors, electrolytes and counter electrodes. Dye as a sensitizer is has an important role in DSSC performance improvement. This study aims to obtain optical characterization such as absorbance spectrum and transmittance from DSSC using dye DN-F01 with concentration 2x10<sup>-3</sup> M. Optical characterization has been tested using a UV-VIS Spectrophotometer Shimadzu UV-1800. The test results show that the absorbance spectrum of the dye is at a wavelength of 400-500 nm. Band gap energy of DN-F01 has obtained from its absorbance and transmittance value is about 2,46 eV.</p>

DEVELOPMENT AND VALIDATION OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF DULOXETINE

Objective: The aim of the present work was to develop and validate a simple UV spectroscopic method for the determination of duloxetine, which is a thiophene derivative and a selective neurotransmitter reuptake inhibitor for serotonin, norepinephrine, and to lesser degree dopamine.
 Methods: The UV Spectrophotometric analysis was performed using Shimadzu UV-1800 and Shimadzu UV-1700 spectrophotometer by using solvent system acetonitrile and water in the ratio of 8:2. Detection was performed at a wavelength of 290 nm. Method validation was carried out according to ICH Q2R1 guidelines by taking the parameters linearity, accuracy, precision, ruggedness, and robustness, LOD and LOQ.
 Results: The UV Spectrophotometric method was found linear in the range of 10-50 μg/ml. The method was rugged and robust with % relative standard deviation less than 2. The extraction recoveries were found to be higher than 99% in all experimental conditions.
 Conclusion: Based upon the performance characteristics, the proposed method was found accurate, precise and rapid and suitable for the determination of Duloxetine for routine analysis.

ASTAXANTHIN EXTRACT UTILIZATION IN THE TECHNOLOGY OF PRODUCING COOKED SAUSAGES WITH A LOW CONTENT OF SODIUM NITRITE

The priority direction for the development of meat products technology is the development of recipes for cooked sausages with low residual sodium nitrite content. The search for substances of natural origin capable of influencing the formation of the cooked sausages color and exercising antioxidant properties is an urgent task today. In the submitted practice, an extract of astaxanthin of industrial production (China) was used. The antioxidant activity of the astaxanthin extract was traced by the DPPH method (2, 2-diphenyl-1-picrylhydrazyl) at 517 nm with the Shimadzu UV-1800 spectrophotometer. The content of myoglobin was determined from the optical density of the cooked sausage pigment extract, obtained after extraction with an aqueous solution of acetone, at a wavelength of 540 nm.Astaxanthin in concentrations of 0.08 and 0.1% positively affected the process of color formation and the preservation of fats in the meat formula of cooked sausages. It was determined that, before administration to the meat formula, the extract of astaxanthin should be dissolved in vegetable oil and left for 3 hours at room temperature. Thus, it's uniform spacing over the stuffing (forcemeat) is achieved without the formation of “red spots”. Astaxanthin is quite stable in meat formulas and gives the cooked meat products a familiar pink color. It is determined that the extract of astaxanthin is recommended for use in the technology of cooked sausages in concentrations of 0.08-0.1% to the mass of the forcemeat.

New first derivative spectrophotometric methods for the estimation of Bumetanide in tablet dosage forms

First derivative spectrophotometric methods have been developed for the assay of Bumetanide using Shimadzu UV-1800 Model double beam UV-VIS spectrophotometer in reagents such as hydrochloric acid, phosphate buffer pH 3.6, phosphate buffer pH 2.0 and distilled water. Bumetanide has shown linearity 10-70 µg/ml in all the methods and the linear regression equations are found to be y = 0.0015x - 0.0013 (R² = 0.9993), y= 0.0007x - 0.0004 (R² = 0.9991), y = 0.0014x - 0.0015 (R² = 0.9993) and y = 0.0014x - 0.0015 (R² = 0.9995) in hydrochloric acid, phosphate buffer pH 3.6, phosphate buffer pH 2.0, and distilled water respectively. The methods are validated as per ICH guidelines and found to be precise, simple and accurate.

Structural and Optical Properties of Nd3+ Doped Lead Borotellurite Glass Containing Silver

Nd3+ doped lead borotellurite glasses which contained silver had been prepared successfully by using conventional melt-quenching method with the chemical composition of (69.5-x)TeO2-20B2O3-10PbO-0.5Nd2O3-xAgNO3 (where x = 0, 0.5, 1.0, 1.5, 2.0 and 2.5 mol%) and varied silver nitrate content. The structural properties had been studied through X-ray diffraction (XRD) analysis and Fourier Transform Infrared (FTIR) spectroscopy. Meanwhile, the optical properties of the glass samples had been characterised by Shimadzu UV-1800 UV-Vis spectrophotometer in the range of 190 – 1100 nm where the absorption peaks were obtained and band assignments of each peak were determined from the absorption spectra. The XRD pattern had been used to confirm the amorphous nature of the glass samples. There was no sharp peak being observed in XRD pattern of the glass samples which had confirmed the amorphous nature of the glass. FTIR spectra were used to analyse the functional groups that existed in the glass samples. They had revealed the presence of or , , , BO4 and the characteristics of the hydrogen bond in the prepared glass samples.Â

DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF BENIDIPINE HYDROCHLORIDE BY USING QUALITY BY DESIGN (QbD) APPROACH

Objective: To develop a simple, rapid, accurate, robust and inexpensive spectrophotometric method for the estimation of benidipine hydrochloride by using quality by design (QbD)” approach.Methods: A UV spectrophotometric method was developed on Shimadzu UV-1800 double beam spectrophotometer using methanol as solvent and wavelength of 236 nm was selected as absorbance maxima (𝜆max). Effect of input variables on spectrum characteristics were studied for the selection of critical parameters and proposed method was validated for various parameters like system suitability, linearity, precision, accuracy, detection limits and quantification limits as per the International Conference on Harmonization guidelines ICH Q2(R1).Results: Linearity of the method was found to be excellent over the concentration range 3 to 18 µg/ml with high correlation coefficient value of 0.9999. Limits of detection and quantification were found to be 0.20 µg/ml and 0.60 µg/ml respectively. The mean recovery was found to be 100.35 % with low percentage relative standard deviation (% RSD) value. The precision study also has shown low % RSD value (<1). No interfering peaks were observed during specificity studies.Conclusion: Obtained result indicated that the developed spectrophotometric method is robust and efficient for the determination of benidipine hydrochloride.

The influence of addition of papain enzyme and Carboxyl Methyl Cellulose on the textural properties of Tofu

Papain enzyme and carboxyl methyl cellulosa was used in tofu production as coagulant and thickener. Papain enzyme is a protease enzyme that can break proteins. Papain enzymeuseful as coagulant to replace acid and base coagulant. The goal of this study is to observe papain enzyme as coagulant and carboxyl methyl cellulose as thickener to increase characteristic of tofu. Tofu is from soy milk has been pasteurized at 70 °C with the addition of papain enzyme and carboxyl methly cellulose. The concenration of papain enzyme is varied such as 200, 400, 800, and 1000 ppm. After Temperature reachs at 90 °C, carboxyl methyl cellulosa is added in soy milk to produce tofu. This study focuses on introducing papain enzyme as coagulant as well as investigating its potential in improving tofu making process productivity. Further the present work attempts to determine the synergistic effect of combining CMC/enzyme in tofu characteristic. This research was conducted with soy milk pasteurized at 70 °C with increasing papain enzyme. Protein from tofu was determined by using Spectrophotometer UV-VIS Shimadzu UV-1800 type. The highest protein concentration of the papain enzyme was found in 1000 ppm with CMC concentration of 0.6% w/v and based on organoleptic tests that the adding CMC and enzyme papain does not effect the taste, smell, texture and color of tofu. The taste of tofu produced is similar to the taste of tofu in the market.

A simple homemade spectrophotometer

A simple portable spectrophotometer was constructed and used to perform analytical experiments. The developed model uses available tools and materials such as light emitting diode lamps, compact discs, plastic and cardboard boxes and a cell phone camera to build a design that illustrates the major components of spectrophotometers. The spectra of serial concentrations of KMnO4 (50‒600 μM) were recorded and the values of absorbance were extracted at λmax to build the calibration curve. A linear relationship between the concentration and absorbance was obtained with the coefficient of determination 0.994. The model was also utilized to study the spectra of KMnO4, phenolphthalein and bromothymol blue in comparison with a Shimadzu UV-1800 spectrophotometer as a reference instrument. In spite of the differences in the observed spectra, the recorded λmax were almost identical to those measured by the developed model. The model was successfully used to determine the concentration of sodium alendronate through ligand-exchange complexation with ferric salicylate.

The Influence of some Prefermentative Treatments on Compositional and Organoleptic Features of Fetească Albă Wines

Wine is a product resulting from the crossing of the conditions offered by plantation and the different treatments applied by winemakers in the wine making process. The present study has evaluated the influence of pre- fermentative treatments on the physical- chemical and chromatic features of the analyzed wines, and it was also observed the differences on the sensory level. Six experimental samples were obtained: FA V0- control sample (no pre- fermentative treatments), FA V1 (pectinase and I²- glucosidase treatment), FA V2 (bentonite treatment), FA V3 (glutathione treatment), FA V4 (tannin treatment), FA V5 (gelatin and tannin treatment). The physical-chemical analyses were done according to the Compendium of International Methods of Analysis of Wine and Musts. A Shimadzu UV-1800 spectrophotometer was used to examine chromatic features, the total polyphenol index and the Folin- CiocA¢lteu index. The sensory evaluation was conducted according the evaluation method originally proposed by the International Union of Oenologists (UIO). As expected, the pre-fermentative treatments didn’t have a significant influence on the physical-chemical features of the wines, but important differences were identified on the sensory level and concerning the polyphenol indices.

The Solvent Effects on the Formation of Polyynes by Laser Ablation

(≥ 99.0%), n-octane (≥ 99.0%). These solvents were purchased from Sigma-Aldrich and used without further purification. The surface of the graphite target was polished with 1200grade emery paper prior to laser irradiation using a Qswitched Nd:YAG laser (Continuum, Surelite I, λ = 1064 nm, pulse duration 5 ns) operated at 10 Hz. The energy of the laser was maintained at 20 mJ/pulse in order to avoid generation of splashes. The laser beam was loosely focused using a lens with a focal length of 300 mm and the typical irradiation time was 20 min. The solution was stirred during the ablation using a magnetic stirrer. The target was also continuously rotated to minimize the effect of target aging and to provide some stirring effect. The optical properties of the nascent polyynes solutions were studied at room temperature in various solvents by a UV-vis absorption spectrophotometer (Shimadzu UV1800) and normal Raman spectra were recorded by a spectrophotometer (HORIBA T64000).