Scalp care and scaling are used for prevention of hair damage and alopecia. In this study, efficacy of 5 natural materials (shiitake mushroom, buckwheat, soybean, cypress, and seaweed fulvescens) was investigated for using of scalp care. Natural materials analyzed general ingredient, and ethanol extracts of natural materials were proven cell viability and closed patch test. Antioxidative effect (scavenging activity of DPPH and SO) and antibacterial activity were measured, and usability of prepared toner with natural materials ethanol extract was questioned. As a result, shiitake mushroom had 14.00%, buckwheat with 5.52%, soybean with 41.51%, and cypress with 24.27% of crude protein, and seaweed fulvescens had 33.67% of crude ash in the general ingredient analysis. In safety evaluation, 50% of cell viability from 5 natural materials ethanol extract was shown in 91~152 μL/mL, and blister (-), redness (-), allergic reaction (-) were not observed after 24 hours closed patch test. When scavenging activity of DPPH and SO was compared with the positive control substance BHT 0.1 mg/mL, shiitake mushroom was 25 μL/mL and 50 μL/mL, buckwheat was 50 μL/mL and 25 μL/mL, soybean was 25~50 μL/mL and 50 μL/mL, cypress was ‹25 μL/mL and ›50 μL/mL, seaweed fulvescens was 25 μL/mL and 50 μL/mL. Good antioxidative activities from shiitake mushroom and buckwheat extracts were observed. Cypress extracts showed good antimicrobial activities against S. aureus and S. epidermidis with concentration of 150 μL/mL. For the usability evaluation, there were positive responses with ‘good’ and ‘average’ on 5 questions. In conclusion, it was suggested that shiitake mushroom, buckwheat, soybean, cypress, and seaweed fulvescens can be used as cosmetics materials for scalp scaling with its high safety, usability, and efficiency.