Abstract
The purpose of this work was to investigate various physiological activities of Lactococcus lactis PK-8 isolated from shiitake mushrooms and to assess the efficacy of enhanced activities by the addition of mushroom extracts. Initially, we characterized the physiological and biochemical properties of this strain. Phylogenetic analysis using 16S rRNA sequencing was utilized for identification, and the strain was assigned and designated as L. lactis PK-8. Phylogenetic tree of the strain was plotted based on 16S rRNA sequence comparisons. Various physiological activities (e.g., tyrosinase inhibitory activity, ACE inhibitory activity, antioxidant activity, SOD-like activity, depletion of sodium nitrite, antibacterial acticity) of L. lactis PK-8 cultures with 10% mushroom extracts were evaluated and compared to the cultures without the extracts. According to the results, all of the physiological activities evaluated in this work were considerably higher in L. lactis PK-8 cultures with 10% mushroom extracts than in the cultures without the extracts. In consequence, this study demonstrated that shiitake mushroom extracts would be effective for enhancing the physiological activities of L. lactis PK-8.
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