Dietary Shifts Research Articles

Implication of lipid turnover for the control of energy balance.

The ongoing obesity epidemic is a consequence of a progressive energy imbalance. The energy-balance model (EBM) posits that obesity results from an excess in food intake and circulating fuels. A reversal in causality has been proposed recently in the form of the carbohydrate-insulin model (CIM), according to which fat storage drives energy imbalance. Under the CIM, dietary carbohydrates shift energy use in favour of storage in adipose tissue. The dynamics of lipid storage and mobilization could, therefore, be sensitive to changes in carbohydrate intake and represent a measurable component of the CIM. To characterize potential changes in lipid dynamics induced by carbohydrates, mathematical models were used. Here, we propose a coherent mathematical implementation of the CIM-energy deposition model (CIM-EDM), which includes lipid turnover dynamics. Using lipid turnover data previously obtained by radiocarbon dating, we build two cohorts of virtual patients and simulate lipid dynamics during ageing and weight loss. We identify clinically testable lipid dynamic parameters that discriminate between the CIM-EDM and an energy in, energy out implementation of the EBM (EBM-IOM). Using a clinically relevant two-month virtual trial, we additionally identify scenarios and propose mechanisms whereby individuals may respond differently to low-carbohydrate diets. This article is part of a discussion meeting issue 'Causes of obesity: theories, conjectures and evidence (Part II)'.

A new climate externalities food knowledge test validated by item response theory and behavioural data prediction (Validación de una nueva prueba de conocimientos sobre la externalidad climática de los alimentos mediante la teoría de respuesta al ítem y la predicción a partir de datos conductuales)

ABSTRACT Research underscores the imperative for global dietary shifts to curb global temperature rise within the Paris Agreement’s 2°C limit. Understanding human dietary behaviour is crucial for effective environmental policies promoting pro-environmental actions. A significant obstacle is the lack of awareness regarding the CO2 implications of meat and dairy consumption, hindering sustainable diet adoption. Thus, evaluating knowledge-based interventions requires reliable tools to quantify climate impact knowledge related to food consumption. Unfortunately, existing pro-environmental knowledge measurement instruments lack domain specificity, leaving a void in assessing climate-conscious food choice knowledge. Addressing this void, our climate externalities food knowledge test was developed. It underwent calibration via item response theory with a German university student sample and validation across one German and two Chilean student samples. The resulting scale exhibited a strong approximate model fit, acceptable item fit metrics, good reliability and the ability to predict climate-relevant food-choice patterns and expert group performance. However, notable differential item functioning was observed based on cultural context. Our instrument provides a valuable contribution to understanding and promoting sustainable food choices on a global scale.

The Risk Factors and Interventions of Dental Caries in the Elderly: A Narrative Review

As the human lifespan increases, older adults are known to retain their teeth for longer. Dental clinicians will see an increase in the number of elderly patients as the population’s life expectancy rises. The loss of periodontal attachment is a common risk factor for root surface caries development in older adults. Other risk factors include an increase in the number of missing teeth and restorations, prosthesis wearing, reduced manual dexterity, a shift in diet from complex to simple sugars, salivary gland dysfunction and increased salivary glucose levels. Dental caries is one of the causes leading to tooth loss in older adults and tooth loss significantly impacts the oral health-related quality of life for this vulnerable age group. Furthermore, the treatment of root caries is complicated by the adhesion of restorative materials to dentin and cemental layer. To curb these issues, understanding the risk factors for dental caries in the elderly, as well as its prevention and treatment strategies, is therefore crucial. This narrative review aims to provide an understanding of dental caries in older adults and an evidence-based prevention and management of this dental disease.

Alternative Proteins in Low- and Middle-Income Countries Face a Questionable Future: Will Technology Negate Bennett’s Law?

Rising incomes across low-and middle-income countries (LMIC) lead to a lower consumption of starchy staples and create a growing demand for high-quality animal protein, an observation referred to as Bennett’s law. This dietary shift from plant-sourced to animal-sourced proteins has also been referred to as the LMIC protein transition. At this time, there are rising concerns that current livestock production is highly resource intensive and may not meet the growing global demand for high-quality protein. Alternative plant-based proteins, derived from new technologies and often fortified with micronutrients, are intended to close the LMIC nutrient gap. However, data from LMIC suggest that the income-driven selection of animal proteins is aspirational and varies by stage of economic development. Food balance sheets from higher-income countries indicate that meat consumption peaks only at very high incomes. Will plant-based alternative proteins satisfy the growing LMIC demand for animal-sourced foods, thereby negating Bennett’s law? Current evidence suggests otherwise.

Golf course living leads to a diet shift for American alligators.

Human-driven land use change can fundamentally alter ecological communities, especially the diversity and abundance of large-bodied predators. Yet, despite the important roles large-bodied predators play in structuring communities through feeding, there have been only a few investigations of how the feeding patterns of large-bodied predators change in human-dominated landscapes. One group of large-bodied predators that has been largely overlooked in the context of land use change is the crocodilians. To help fill these gaps, we studied the feeding patterns of juvenile American alligators (Alligator mississippiensis) on neighboring barrier islands on the southeast coast of Georgia, USA. Jekyll Island has multiple golf courses and substantial amounts of human activity, while Sapelo Island does not have any golf courses and a much smaller amount of human activity. We found that juvenile alligator populations on both islands ate the same types of prey but in vastly different quantities. Sapelo Island alligators primarily consumed crustaceans while alligators that lived on Jekyll Island's golf courses ate mostly insects/arachnids. Furthermore, the Jekyll Island alligators exhibited a much more generalist feeding pattern (individuals mostly ate the same types of prey in the same quantities) than the more specialized Sapelo Island alligators (diets were more varied across individuals). The most likely explanation for our results is that alligators living on golf courses have different habitat use patterns and have access to different prey communities relative to alligators in more natural habitats. Thus, land use change can strongly alter the feeding patterns of large-bodied predators and, as a result, may affect their body condition, exposure to human-made chemicals, and role within ecological communities.

Ecotoxicology of mercury concentrations in arctic lamprey (Lethenteron camtschaticum)

Arctic lamprey (Lethenteron camtschaticum) is an important dietary resource for rural and indigenous communities in parts of Alaska, with some commercial use. As with many fish species harvested for human consumption, there are concerns regarding mercury concentrations ([Hg]) in Arctic lamprey that may impact human health. To date, information regarding the life cycle and diet of Arctic lamprey is scarce, with no published studies examining [Hg] in Arctic lamprey tissues. Our goals were to investigate the feeding ecology of Arctic lamprey from the Bering Sea, determine how diet and potential dietary shifts might influence [Hg] in muscle, and determine if current [Hg] may pose a human health risk. The mean total [Hg] in Arctic lamprey muscle (n = 98) was 19 ng/g wet-weight. Log transformed total [Hg] were not associated with any measured biological variables including length, mass, δ13C values, or δ15N values. A stable isotope mixing model estimated that capelin (Mallotus villosus) accounted for 40.0 ± 4.0% of the Arctic lamprey diet, while Pacific sand lance (Ammodytes hexapterus) and Pacific herring (Clupea pallasii) accounted for 37.8 ± 3.1% and 22.2 ± 3.5% respectively. Finally, diet percentage compositions shifted based on size class (i.e., medium versus large). These results indicated that feeding location, bioaccumulation, and biomagnification are not important drivers of [Hg] in Arctic lamprey and current [Hg] do not pose a human health risk. Taken together, this research further expands our knowledge of Arctic lamprey trophic ecology in the eastern Bering Sea.

Utilization of Sasa borealis seeds by Japanese field mouse: discovery of small-seed caching

Bambusoideae are well-known for their large-scale flowering, seeding, and death events that occur after a vegetative phase that can often last for decades. These events are a valuable resource for many animals. Their mast seeding, in particular, affects rodent populations although the causal relationship between them is speculative. Moreover, the species of animals that utilize this resource also remains unclear. Therefore, this study aimed to examine the interaction between mast seeding plants and animals by conducting a feeding test using the seeds of a dwarf bamboo species (Sasa borealis) at different seasons and in different forest types. The results revealed that two mouse species, Apodemus speciosus and A. argenteus, utilized the seeds. They both predated as well as removed and sometimes buried the seeds, exhibiting small-seed caching behavior. Furthermore, A. speciosus was found to exhibit higher seed predation at covered sites and switching to removal behavior at the more exposed, open sites, while A. argenteus showed no distinguishable trends. Additionally, A. speciosus utilized the seeds less frequently in fall than in summer, suggesting a shift in diet towards acorns when available. Collectively, the results of this study provide novel insights into the interaction between mast seeding plants and the animals that utilize them.

Feeding ecology and reproductive biology of small coastal sharks in Malaysian waters.

Small coastal demersal sharks form a major proportion of the sharks landed in Malaysia. However, little is known about their feeding ecology and reproduction. This study sought to elucidate the dietary patterns, role of ontogeny in prey consumption, and reproductive biology of four dominant small demersal shark species in Malaysian waters: the Hasselt's bamboo shark, Chiloscyllium hasseltii; brownbanded bamboo shark, C. punctatum; spadenose shark, Scoliodon laticaudus; and Pacific spadenose shark, S. macrorhynchos. Dietary analyses revealed a high overlap in prey taxa consumed; clear resource partitioning among co-occurring species based on the percentage Prey-specific Index of Relative Importance (%PSIRI), with higher fish %PSIRI for Chiloscyllium hasseltii, higher cephalopod %PSIRI for C. punctatum, and higher crustacean %PSIRI for both Scoliodon species; and an ontogenetic diet shift, seen through changes in prey size. Based on the examination of reproductive organs, the results showed larger sizes at maturity for males compared to females for all four species; no obvious reproductive cycles, based on hepatosomatic and gonadosomatic indices for all species; female bias in the sex ratio of the embryos of Scoliodon species; and increased reproductive output (number of eggs or embryos and size of eggs) with larger female size for C. hasseltii and Scoliodon species. The partitioning of food resources minimizes direct competition for food and supports coexistence within shared coastal habitats. The reproductive strategies of these small coastal sharks appear to be favorable for supporting short-term population productivity; although a reduction in fishing pressure, especially from bottom trawlers, is essential for the long-term sustainable use of these sharks.

Development of Gut Microbiota in the First 1000 Days after Birth and Potential Interventions.

The first 1000 days after birth represent a critical window for gut microbiome development, which is essential for immune system maturation and overall health. The gut microbiome undergoes major changes during this period due to shifts in diet and environment. Disruptions to the microbiota early in life can have lasting health effects, including increased risks of inflammatory disorders, autoimmune diseases, neurological disorders, and obesity. Maternal and environmental factors during pregnancy and infancy shape the infant gut microbiota. In this article, we will review how maintaining a healthy gut microbiome in pregnancy and infancy is important for long-term infant health. Furthermore, we briefly include fungal colonization and its effects on the host immune function, which are discussed as part of gut microbiome ecosystem. Additionally, we will describe how potential approaches such as hydrogels enriched with prebiotics and probiotics, gut microbiota transplantation (GMT) during pregnancy, age-specific microbial ecosystem therapeutics, and CRISPR therapies targeting the gut microbiota hold potential for advancing research and development. Nevertheless, thorough evaluation of their safety, effectiveness, and lasting impacts is crucial prior to their application in clinical approach. The article emphasizes the need for continued research to optimize gut microbiota and immune system development through targeted early-life interventions.

A stable isotope analysis of the dietary patterns of the aquatic apex predator, the African tigerfish (Hydrocynus vittatus).

Stable isotope analyses, specifically δ13 C and δ15 N, are useful tools increasingly used to understand ecosystem function, food web structures and consumer diets. Although the iconic tigerfish, Hydrocynus vittatus, is regarded as an apex predator in southern African freshwater systems, little information is available regarding their feeding behaviour and how this may change with growth or differ between ecosystems with most information stemming from stomach content analyses (SCA). The aim of the present study was to address this lack of information through a baseline study of the diet of large and small tigerfish in various lentic and lotic ecosystems in South Africa using stable isotope methods. Fish and various food web components and food sources were collected from two river and two lake ecosystems from the Lower Phongolo River floodplain, SA. The δ13 C and δ15 N values for all samples were determined and multivariate analyses and Bayesian analytical techniques applied to determine the feeding ecology of H. vittatus and how this may differ with size and habitat type. Analyses revealed a substantial difference in the type and abundance of food sources contributing to the diet of H. vittatus between ecosystems, most prominently between the lotic systems, where less dietary specialisation was observed, and lentic systems where more specialisation was observed. Furthermore, there was a distinct difference in diet between small and large tigerfish, especially in the lotic system, indicating an ontogenetic diet shift as tigerfish grow and further supporting previous SCA studies. This is the first study of its kind on the African continent for H. vittatus and the findings illustrate the value of stable isotope analysis in providing in-depth information into the feeding ecology of consumers and how this may differ between size classes and habitat types. This article is protected by copyright. All rights reserved.

A nutrition-defence trade-off drives diet choice in a toxic plant generalist.

Plant toxicity shapes the dietary choices of herbivores. Especially when herbivores sequester plant toxins, they may experience a trade-off between gaining protection from natural enemies and avoiding toxicity. The availability of toxins for sequestration may additionally trade off with the nutritional quality of a potential food source for sequestering herbivores. We hypothesized that diet mixing might allow a sequestering herbivore to balance nutrition and defence (via sequestration of plant toxins). Accordingly, here we address diet mixing and sequestration of large milkweed bugs (Oncopeltus fasciatus) when they have differential access to toxins (cardenolides) in their diet. In the absence of toxins from a preferred food (milkweed seeds), large milkweed bugs fed on nutritionally adequate non-toxic seeds, but supplemented their diet by feeding on nutritionally poor, but cardenolide-rich milkweed leaf and stem tissues. This dietary shift corresponded to reduced insect growth but facilitated sequestration of defensive toxins. Plant production of cardenolides was also substantially induced by bug feeding on leaf and stem tissues, perhaps benefitting this cardenolide-resistant herbivore. Thus, sequestration appears to drive diet mixing in this toxic plant generalist, even at the cost of feeding on nutritionally poor plant tissue.

Seasonal dietary shifts alter the gut microbiota of a frugivorous lizard Teratoscincus roborowskii (Squamata, Sphaerodactylidae).

Seasonal dietary shifts in animals are important strategies for ecological adaptation. An increasing number of studies have shown that seasonal dietary shifts can influence or even determine the composition of gut microbiota. The Turpan wonder gecko, Teratoscincus roborowskii, lives in extreme desert environments and has a flexible dietary shift to fruit-eating in warm seasons. However, the effect of such shifts on the gut microbiota is poorly understood. In this study, 16S rRNA sequencing and LC-MS metabolomics were used to examine changes in the gut microbiota composition and metabolic patterns of T. roborowskii. The results demonstrated that the gut microbes of T. roborowskii underwent significant seasonal changes, and the abundance of phylum level in autumn was significantly higher than spring, but meanwhile, the diversity was lower. At the family level, the abundance and diversity of the gut microbiota were both higher in autumn. Firmicutes, Bacteroidetes, and Proteobacteria were the dominant gut microbes of T. roborowskii. Verrucomicrobia and Proteobacteria exhibited dynamic ebb and flow patterns between spring and autumn. Metabolomic profiling also revealed differences mainly related to the formation of secondary bile acids. The pantothenate and CoA biosynthesis, and lysine degradation pathways identified by KEGG enrichment symbolize the exuberant metabolic capacity of T. roborowskii. Furthermore, strong correlations were detected between metabolite types and bacteria, and this correlation may be an important adaptation of T. roborowskii to cope with dietary shifts and improve energy acquisition. Our study provides a theoretical basis for exploring the adaptive evolution of the special frugivorous behavior of T. roborowskii, which is an important progress in the study of gut microbes in desert lizards.

La Sassa cave: Isotopic evidence for Copper Age and Bronze Age population dynamics in Central Italy.

This study focuses on the changes in diet and mobility of people buried in the La Sassa cave (Latium, Central Italy) during the Copper and Bronze Ages to contribute to the understanding of the complex contemporary population dynamics in Central Italy. To that purpose, carbon and nitrogen stable isotope analyses, strontium isotope analyses, and FT-IR evaluations were performed on human and faunal remains from this cave. The stable isotope analyses evidence a slight shift in diet between Copper and Bronze Age individuals, which becomes prominent in an individual, dating from a late phase, when the cave was mainly used as a cultic shelter. This diachronic study documents an increased dietary variability due to the introduction of novel resources in these protohistoric societies, possibly related to the southward spread of northern human groups into Central Italy. This contact between different cultures is also testified by the pottery typology found in the cave. The latter shows an increase in cultural intermingling starting during the beginning of the middle Bronze Age. The local mobility during this phase likely involved multiple communities scattered throughout an area of a few kilometers around the cave, which used the latter as a burial site both in the Copper and Bronze ages.

Diet variation of common smelt across a salinity gradient in coastal lakes on Rēkohu (Chatham Island)

ABSTRACT Sea level rise threatens ecosystem function in coastal lakes by increasing salinity and altering community composition. As mobile generalists, fish have considerable influence on the stability of a system by impacting trophic network structure. Understanding their diet across abiotic gradients, including salinity, can show key characteristics of their ecological role, and predator diet shifts may serve as warning signs of food web restructuring. Stomach contents analysis was used to look at the diet of Retropinna retropinna (common smelt) across shallow coastal lakes on Rēkohu (Chatham Island). Smelt diets from 10 lakes (seven freshwater; three salt-influenced) were analysed. Comparisons of salt-influenced and freshwater lakes showed shifts in composition. Smelt in freshwater lakes predominantly consumed herbivorous, omnivorous, or predatory insect larvae while diet in salt-influenced lakes was dominanted by crustacean shredders. Population diet varied somewhat less among salt-influenced lakes than among freshwater lakes, suggesting homogenisation of smelt diet with salinity. Differences in composition and heterogeneity did not translate to consistent differences in total niche width or individual specialisation between freshwater and salt-influenced lakes. Further development of knowledge on biological structure across salinity gradients is essential to understand ecosystem function under future climate scenarios.

A three-dimensional geometric morphometric analysis of the morphological transformation of Caiman lower jaw during post-hatching ontogeny.

Shape ontogenetic changes of the lower jaw in crocodylians are poorly understood. In order to answer some questions related to the inter- and intraspecific morphological variation of the mandible of two extant Caiman species, we performed a three-dimensional geometric morphometric approach. For this purpose, we used landmarks and semilandmarks on two ontogenetic mandibular series of 48 and 15 post-hatching specimens of C. yacare and C. latirostris, respectively. We have also examined the relationship between these anatomical transformations and ontogenetic shifts in diet. We performed a principal component analysis (PCA) for the two species, and regression and partial least squares (PLS) analyses for each species, separately. As a result, species were segregated along the PC1 with specimens of C. yacare showing more gracile mandibles, and specimens of C. latirostris more robust ones. The PC2 and regression analyses showed an age gradient and represented ontogenetic shape changes. Adult caiman mandibles are higher and wider than juvenile ones, and shape changes are more conspicuous in C. latirostris. The PLS analyses showed a significant relationship between shape and diet. Morphological changes of the PLS1 of block-1 match with those of the regression analysis for both species. We have detected morphological transformations in areas where the musculature in charge of mandibular movements is attached. Common morphological changes occurring during ontogeny seem to reflect the same mechanical properties required for crushing and killing in both species, driven by an ontogenetic shift in the diet from invertebrates to vertebrates. Additionally, interspecific differences were also found to be correlated to ontogenetic changes in diet and could be related to dissimilar feeding mechanical requirements (e.g., stiffness and toughness of the item consumed), and to different habitat preferences. Robust mandibles would be more suitable for shallow and fully vegetated environments, as it can be seen in C. latirostris, whereas slender jaws seem to be more suitable for more aquatic species such as C. yacare.

Parallel Evolution of Skull Form in Three Rodent Genera Inhabiting Steep Elevational Gradients of Ethiopian Highlands

Several rodent genera radiated in Ethiopian highlands during Pleistocene, giving rise to species specialized to different elevation zones. The zonation is accompanied by vegetation change from savannahs or forests in lower elevations, through the mid-elevation belt of ericaceous shrublands to Afroalpine moorlands on the mountain tops. Such evolution on elevation gradient is expected to promote phenotypic adaptations and several genera are known to include highly distinctive high-elevation specialists. In this study, we systematically examined shape and size differentiation of skull and mandible in three genera, Lophuromys, Arvicanthis and Stenocephalemys, each including from six to eight species inhabiting different elevation zones on both sides of the Great Rift Valley. Employing computed tomography, three-dimensional virtual models, landmark-based geometric morphometrics and a powerful toolkit of partial least squares analysis, we demonstrated the elevation-related differentiation is a prominent feature of skull and mandible shape variation in all three genera. We identified the parts of skull and mandible which change most between the successive zones. These include, notably, orbits and insertion areas of masticatory muscles, which suggests adaptive shifts in visual perception and diet. We also showed that while the same parts are modified in all three genera, they are modified in different ways and to a different degree. The evidence for size differentiation was mixed despite the universal trend of larger size in higher elevations. Our study confirms the high-elevation specialists represent the most distinctive forms, which is especially true for the species from Bale and Arsi Mountains, East of the Great Rift Valley.

Using mixing models to study human paleodiets in central‐western Santa Cruz (Argentina) during Late Holocene

AbstractDuring the final Late Holocene, there was a socio‐economic reorganization among the people in the central‐western of Santa Cruz, Argentina. Analysis of bone collagen revealed that hunter–gatherer groups maintained a consistent diet rich in steppe animal protein for the past 3000 years. However, there was a change in bioapatite δ13C values in the last 1000 years, indicating a shift in the whole diet that was not reflected in the protein component. This article seeks to evaluate the differential consumption of resources over time and between sexes among hunter–gatherer populations in the region. A total of 39 adult individuals of both sexes, recovered from stone burial structures with different chronologies, were analyzed. Quantitative diet reconstruction was achieved through the use of the Bayesian mixing model known as Food Reconstruction Using Isotopic Transferred Signals (FRUITS). The results show that the guanaco (Lama guanicoe) was the most consumed animal throughout the studied period, considering both temporal and sex differences. Additionally, there is an increased importance of plant consumption in the last millennium, particularly among females. However, this can be the result of overrepresentation of this resource in our food web samples, due to the impossibility of resolving the origin of the carbon for the formation of bioapatite values.

Stable isotopic and stomach content analyses reveal changes in the trophic level and feeding habit of large-head hairtail (Trichiurus lepturus) in the northern South China Sea

The feeding habit of large-head hairtail (Trichiurus lepturus) in the northern South China Sea was investigated through isotopic and stomach content analyses. The isotopic features of the hairtail at the same body size differed among regions, with the fish in coastal waters presenting higher δ15N and δ13C values compared to those in the open sea, indicating different trophic levels (TL), food habits, and isotopic baselines. According to the partial correlation of water depth with δ15N values, the sampling stations were divided into three regions based on the depth of water: coastal (20–40 m), near coastal (60–80 m), and open sea (100–200 m) regions. In the coastal region, the hairtail from stations affected by the Pearl River plume exhibited lower δ15N and δ13C values. The stomach content analysis indicated different feeding habits of the hairtail from different regions. The hairtail in the coastal and near coastal waters fed more on fish and less on crustaceans compared to the hairtail in the open sea. The relationship between δ15N and fish size exhibited two contrary patterns. First, the δ15N values increased with increasing preanal length in the hairtail sampled from the water depth of 30–40 m in section F (in fish with preanal length < 200 mm) and those samples from the water depth of 100–200 m. This finding reflected an ontogenetic shift in diet and TL. However, the δ15N values tended to decrease with the increasing preanal length of the hairtail samples collected from the water depth of 30–40 m in section F (fish with a preanal length of ~200–300 mm). These findings suggested that under the conditions of insufficient availability of high-quality prey, the larger hairtail fed more on low-TL prey to compensate for the increase energy demand, arising due to growth, which led to the observed decrease in δ15N values.

Human subsistence and signatures of selection on chemosensory genes

Chemosensation (olfaction, taste) is essential for detecting and assessing foods, such that dietary shifts elicit evolutionary changes in vertebrate chemosensory genes. The transition from hunting and gathering to agriculture dramatically altered how humans acquire food. Recent genetic and linguistic studies suggest agriculture may have precipitated olfactory degeneration. Here, we explore the effects of subsistence behaviors on olfactory (OR) and taste (TASR) receptor genes among rainforest foragers and neighboring agriculturalists in Africa and Southeast Asia. We analyze 378 functional OR and 26 functional TASR genes in 133 individuals across populations in Uganda (Twa, Sua, BaKiga) and the Philippines (Agta, Mamanwa, Manobo) with differing subsistence histories. We find no evidence of relaxed selection on chemosensory genes in agricultural populations. However, we identify subsistence-related signatures of local adaptation on chemosensory genes within each geographic region. Our results highlight the importance of culture, subsistence economy, and drift in human chemosensory perception.

Sub-zero temperatures and large-scale weather patterns induce tooth damage in Icelandic arctic foxes.

Tooth damage in carnivores can reflect shifts in both diet and feeding habits, and in large carnivores, it is associated with increased bone consumption. Variation in tooth condition in Icelandic arctic foxes, a mesocarnivore, was recorded from 854 individual foxes spanning 29 years. We hypothesized that annual climatic variations, which can influence food abundance and accessibility, will influence tooth condition by causing dietary shifts toward less edible prey. We examined tooth condition in relation to four climatic predictors: mean annual winter temperature, indices of both the El Niño anomaly and North Atlantic subpolar gyre (SPG), and the number of rain-on-snow days (ROS). We found unequivocal evidence for a strong effect of annual climate on tooth condition. Teeth of Icelandic foxes were in better condition when winter temperatures were higher, when the SPG was more positive, and when the number of ROS was low. We also found a substantial subregional effect with foxes from northeastern Iceland having lower tooth damage than those from two western sites. Contradicting our original hypothesis that foxes from northeastern Iceland, where foxes are known to scavenge on large mammal remains (e.g., sheep and horses), would show the highest tooth damage, we suggest that western coastal sites exhibited greater tooth damage because cold winter temperatures lowered the availability of seabirds, causing a shift in diet toward abrasive marine subsidies (e.g., bivalves) and frozen beach wrack. Our study shows that monitoring tooth breakage and wear can be a useful tool for evaluating the impact of climate on carnivore populations and that climate change may influence the condition and fitness of carnivores in complex and potentially conflicting ways.