Shelter Cats Research Articles

The influence of degree of socialization and age on length of stay of shelter cats

ABSTRACT Surveys indicate that cat behaviors, such as degree of friendliness or playfulness, are important to potential adopters and cat owners. There are few data, however, relating the degree of socialization in cats with length of stay (LOS) in a shelter. Based on provided definitions, staff from 31 shelters in the Northeastern and Mid-Atlantic United States placed cats (n = 645) into one of three behavioral categories: interactive, approachable, and unapproachable. Age and LOS for each cat were also reported. Using a mixed model analysis that controlled for the effect of shelter, the LOS of interactive cats was 37 days, LOS of approachable cats was 51 days, and LOS of unapproachable cats was 119 days. LOS increased four days, on average, for each additional year of age and there was an interaction between age and behavioral categories. Importantly, LOS of interactive cats was not influenced by age. Future research should explore the effectiveness of cat behavioral modification programs on reducing LOS of shelter cats.

Leptospiral shedding and seropositivity in shelter dogs in the Cumberland Gap Region of Southeastern Appalachia.

BackgroundLeptospirosis, caused by pathogenic Leptospira spp., is a zoonotic infection that affects humans, dogs and many other mammalian species. Virtually any mammalian species can act as asymptomatic reservoir, characterized by chronic renal carriage and shedding of a host-adapted leptospiral serovar. Environmental contamination by chronic shedders results in acquisition of infection by humans and susceptible animals.MethodsIn this study, we investigated if clinically normal shelter dogs and cats harbor leptospires in their kidneys by screening urine samples for the presence of leptospiral DNA by a TaqMan based-quantitative PCR (qPCR) that targets pathogen-associated lipl32 gene. To identify the infecting leptospiral species, a fragment of leptospiral rpoB gene was PCR amplified and sequenced. Additionally, we measured Leptospira-specific serum antibodies using the microscopic agglutination test (MAT), a gold standard in leptospiral serology.ResultsA total of 269 shelter animals (219 dogs and 50 cats) from seven shelters located in the tri-state area of western Virginia, eastern Tennessee, and southeastern Kentucky were included in this study. All cats tested negative by both qPCR and MAT. Of the 219 dogs tested in the study, 26/198 (13.1%, 95% CI: 8.4–17.8%) were positive for leptospiral DNA in urine by qPCR and 38/211 (18.0%, 95% CI: 12.8–23.2%) were seropositive by MAT. Twelve dogs were positive for both qPCR and MAT. Fourteen dogs were positive by qPCR but not by MAT. Additionally, leptospiral rpoB gene sequencing from a sub-set of qPCR-positive urine samples (n = 21) revealed L. interrogans to be the leptospiral species shed by dogs.ConclusionsThese findings have significant implications regarding animal and public health in the Cumberland Gap Region and possibly outside where these animals may be adopted.

Occurrence of gastrointestinal protozoans in cats from Londrina, Paraná, Brazil

Protozoans are widely distributed, and several species may parasitize the digestive tracts of cats (Felis catus), and can be transmitted to humans. The present study aimed to evaluate the species and occurrence of gastrointestinal protozoans in cats in the city of Londrina, Paraná, Brazil. A total of 206 cat fecal samples were tested, of which 141 were from shelter animals, and 65 were from pets owned by local people. Samples were processed by parasitological techniques. Coproparasitological techniques (Willis, Faust and Ziehl-Neelsen) were performed for detection of protozoan parasites. Subsequently, all samples were processed by PCR protocols specific to Toxoplasma gondii, Giardia spp., and Cryptosporidium spp. PCR products from positive samples were selected for sequencing. No samples were found to be positive for Cryptosporidium spp. using the Ziehl-Neelsen technique. Using specific PCR protocols, 1/206 (0.48%) samples tested positive for Cryptosporidium spp. After purification, this one positive sample was sequenced, and it demonstrated a 100% identity match to Cryptosporidium muris. Using specific PCR protocols, 13/206 (9.22%) cat fecal samples tested, including 2/65 (3.08%) pet cat fecal samples, were positive for T. gondii. PCR analysis revealed that 37/206 (17.96%) of cat fecal samples were positive for Giardia spp., including 27/141 (19.15%) of shelter cat fecal samples, and 10/65 (15.38%) pet cat fecal samples (p = 0.5124). When sequenced, these positive samples showed a 100% identity match with Giardia duodenalis. This study demonstrated that infections with Cryptosporidium spp., Toxoplasma gondii, and Giardia duodenalis are present in the population of both pet cats and shelter cats in the city of Londrina. This poses a risk to public health, because these parasites have a high zoonotic potential.

Occupational Health of Animal Shelter Employees by Live Release Rate, Shelter Type, and Euthanasia-Related Decision

ABSTRACTApproximately a quarter of dogs and cats in animal shelters in the United States are euthanized. The stress associated with having to care for animals they subsequently euthanize puts animal shelter workers at a high risk for compassion fatigue, burnout, and even suicide. The aim of the present study was to assess the relationship between a shelter’s Live Release Rate (LRR; defined as the percent of animals that leave the animal shelter with a positive outcome) and the involvement in euthanasia-related decision-making on employees’ mental health. An online nationwide survey was administered to 153 municipal and private shelter workers consisting of the Impact of Event Scale-Revised, the Professional Quality of Life Questionnaire, the Moral Injury Event Scale, and questions relating to their work environment. A multivariate regression model found that compassion satisfaction (p < 0.00001), secondary traumatic stress (p = 0.008), moral injury (p = 0.037), and burnout (p = 0.002) were positively correlated with LRR. This suggests that although job satisfaction is greater in shelters with more positive outcomes, trauma may also be greater. A t-test revealed that employees who euthanize have higher moral injury scores compared with those who do not (t(152) = –2.96, p = 0.0036). A second multivariate regression model found that deciding which animal to euthanize predicted increased secondary traumatic stress (r(151) = –0.058, p = 0.016). Overall, the data show that LRR and decisions surrounding euthanasia play a role in occupational trauma; furthermore, the data provide support for further exploration of moral injury in animal shelter workers.

Endoparasites of household and shelter cats in the city of Rio de Janeiro, Brazil.

Endoparasitic infections are associated with morbidity in cats. This study aimed to investigate the occurrence of endoparasites among cats of different life stages in the city of Rio de Janeiro, Brazil. The samples were analyzed individually by macroscopic exploration for proglottids and centrifugal-flotation. Stool samples were obtained from household cats (n = 57) and shelter cats (n = 336). Endoparasites were detected in 50.64% of the samples. Among household and shelter cats, 21.05% and 55.66% were infected with endoparasites, respectively. In household cats, the most prevalent endoparasites were Ancylostoma spp. (in 25.0%) and Strongyloides spp. (in 25.0%), followed by Toxocara spp. (in 16.67%), Dipylidium caninum (in 16.67%), Cystoisospora spp. (in 8.33%), and Uncinaria spp. + Ancylostoma spp. (in 8.33%). In shelter cats, the most prevalent endoparasite was Ancylostoma spp. (in 29.41%), followed by Cystoisospora spp. (in 26.20%) and Toxocara spp. (in 16.58%), as well as Cystoisospora spp. + Toxocara spp. (in 8.02%); Ancylostoma spp. + Toxocara spp. (in 11.76%); Cystoisospora spp. + Ancylostoma spp. (in 3.74%); Cystoisospora spp. + Toxocara spp. + Ancylostoma spp. (in 3.21%); and Dipylidium caninum + Ancylostoma spp. (in 0.53%). Endoparasitic infections in cats underscore the need for preventive veterinary care and routine coproparasitologic tests.

Epidemiological survey of ringworm outbreak in cat shelter

Dermatophytosis (ringworm) is the most important infectious and contagious skin disease of cats in shelters. Wherein, the diagnosis, identification and treatment of dermatophytosis is not always effective. Here we report an epidemiological study of the outbreak of feline dermatophytosis in animal shelter in Moscow region. At least 11% of cats kept in the shelter were affected. Seventeen cats suspected dermatophytosis were isolated for mycological examination and treatment, 82% of them were culture-positive. These cats received a course of oral terbinafine, with no topical treatment. Of the dermatophyte-positive cats, 36% demonstrated skin lesions, while 64% were asymptomatic. No mycological control of recovery was carried out after treatment. It was found that 44% of clinically recovered cats turned to be asymptomatic carriers. When examining environmental objects (bedding, cat houses, floors, etc.), dermatophytes were isolated from 80% of the samples. The only causative agent of dermatophytosis was Microsporum canis. The MIC (minimal inhibitory concentration) of terbinafine for clinical strains of M. canis was determined to be 0.001-0.002 μg/ml. The study confirms the need for large-scale mycological screening of cats in shelters using DTM-type diagnostic media. Oral therapy alone is not sufficient for effective treatment; it should include the whole-body antifungal treatment. Mycological control of cure is also mandatory.

Species diversity and molecular insights into phlebotomine sand flies in Sardinia (Italy)-an endemic region for leishmaniasis.

This study provides updated information on the distribution of the phlebotomine sand fly species and their genetic characterization in Sardinia, a Mediterranean island where leishmaniasis is endemic. From April to November 2017, sand flies were trapped in five different capture sites using sticky traps and light traps as collection methods, operated nearby sheep, poultry, cat, and dog shelters. Phlebotomine specimens (n = 513) collected were morphologically and molecularly identified as Phlebotomus perniciosus (249/513, 48.5%), Phlebotomus perfiliewi (236/513; 46%), and Sergentomyia minuta (28/513, 5.5%). Sand flies were collected from the second half of May to October confirming the well-defined seasonal activity, which peaks in August in Sardinia. Overall, correlation analyses indicated a significant positive association between the monthly number of sand flies collected and the mean temperature (r = 0.88, rho = 0.87, and tau = 0.69, P < 0.05), while there was non-significant, moderately negative correlation between the monthly number of sand flies collected and the monthly mean relative humidity and wind (r = - 0.22, rho = 0.02, and tau = 0.04, P > 0.05). This study provides the first data on the molecular characterization of phlebotomine sand flies in this region and confirms the presence of three sand fly species. Molecular results suggest that the morphological features used for analysis represent synapomorphic-derived characters which are shared among descendant taxa and the common ancestor.

Serosurvey of Rickettsia spp. in cats from a Brazilian spotted fever-endemic area.

Rickettsia spp. bacteria are responsible for tick-borne diseases worldwide, mostly maintained by rickettsial amplifiers capybaras in Brazilian endemic areas. The campus of the University of São Paulo, in southeastern Brazil, is an area endemic for Brazilian spotted fever (BSF), with high density of capybaras and Amblyomma spp., along with confirmed human cases. Besides capybaras, the university has also an in-campus high population of sheltered and free-roaming cats. Accordingly, the aim of this study was to determine the prevalence and characteristics associated with Rickettsia rickettsii, Rickettsia parkeri and Rickettsia felis exposure among cats in a BSF-endemic area. Out of 51 cats sampled, 23/35 shelter (65.7%) and 5/16 free-roaming (31.2%) were positive (titers ≥ 64) for at least one Rickettsia species. Ticks species were present in 3/16 free-roaming cats (18.8%), consisting of Amblyomma spp., nymphs of Amblyomma sculptum and adult Rhipicephalus sanguineus sensu lato. Despite sharing the capybaras environment, the seropositivity among the free-roaming and shelter cats was lower than owned cats in other endemic areas. Whether equally or less exposed to rickettsial infection, compared with owned cats in endemic areas, free-roaming and shelter cats may be used as environmental sentinels for human exposure to rickettsiae in such areas.

Are cats less stressed in homes than in shelters? A study of personality and faecal cortisol metabolites

Personality is defined by characteristics of individuals and describes and accounts for temporally stable patterns of affect, cognition and behaviour traits. The study of cat behaviour and personality can minimize potential problems in the relationship between cats and their owners and decrease abandonment and maltreatment. People generally adopt animals according to the individual’s appearance, age or sex. Personality assessments can help make adoptions successful by identifying ideal subjects for potential owners. A personality assessment called “Meet Your Match®” (MYM), developed by the American Society for the Prevention of Cruelty to Animals (ASPCA) and validated for our cat sample was used in this study. To evaluate stress, we measured faecal cortisol metabolites (FCMs) of cats in shelters and after adoption. In an effort to improve adoption and cat adaptation in new homes, our goals were 1) to evaluate the relationship between personality and cortisol levels; 2) to confirm if MYM assessment is consistent through situations; and 3) to investigate how moving from the shelter to the owners’ homes affects FCM levels. The subjects for our first goal were 53 sheltered cats. For the second and the third goals, we followed 15 of the original 53 after the adoption. No correlation was found between personality dimensions (agreeableness p = 0.878; openness p = 0.141; extraversion p = 0.942) and FCM levels. MYM assessment was consistent through different localities. There was a slight, but significant (p = 0.0072), decrease of FCM levels at owners’ home. However, most subjects (n = 11) did not present changes that were significantly different from zero. Our study underlined the usefulness of the MYM personality assessment and confirmed a lack of correlation between personality and cortisol levels in cats. It is a further step towards incorporating a more objective approach to the adoption process in shelters to improve the pairing of humans and cats.

Population Characteristics of Cats Adopted from an Urban Cat Shelter and the Influence of Physical Traits and Reason for Surrender on Length of Stay.

Simple SummaryEvery year, millions of cats are admitted to shelters around the world. Reducing the amount of time that cats stay in a shelter environment (length of stay, LOS) promotes animal welfare by reducing illness and stress, as well as supporting more efficient use of shelter resources. Understanding the factors that might influence LOS supports evidence-based interventions aimed at improving the flow of animals through shelters. Whether the same factors affect LOS in shelters of different types and from different geographic regions is poorly understood. We studied cats adopted from an urban shelter in Sydney, Australia, and found that stray cats have a longer LOS than owner-relinquished cats, supporting the results of previous studies. Surprisingly, in contrast to the widely held view that black cats stay in shelters longer than white cats, the opposite was true here—overall, white cats stayed longer than black cats in the shelter, even when other factors such as age were taken into consideration. Shelters might consider analyzing their own data, where possible, to inform strategies to reduce LOS.Measures aimed at reducing the length of stay (LOS) of cats in shelters can promote animal welfare and more efficient use of resources. The extent to which variables shown to impact LOS are broadly applicable is unclear. The aim of this study was to describe a population of cats adopted from an urban shelter, and to analyze the association between potential predictor variables and LOS. A study cohort was identified retrospectively from shelter records (n = 2584), 48.8% of which were < 12 weeks old at admission, and 80.7% were stray. Among 445 cats relinquished by owners, reasons for surrender were primarily owner-related (87.2%). Overall, reason for surrender and coat color were significantly associated with LOS. Hazard ratios showed that all reasons for surrender for owner-relinquished cats were associated with a shorter LOS than stray cats and this association was significant (p < 0.05) for all except cat behavioral or medical reasons. In contrast to previous reports, white cats had a significantly (p < 0.05) longer LOS than black cats. This study highlights an important role for shelter-specific baseline data to inform and measure the effect of interventional studies aimed at improving animal welfare by reducing LOS in shelter-housed cats

Pan-European Study on the Prevalence of the Feline Leukaemia Virus Infection - Reported by the European Advisory Board on Cat Diseases (ABCD Europe).

Feline leukaemia virus (FeLV) is a retrovirus associated with fatal disease in progressively infected cats. While testing/removal and vaccination led to a decreased prevalence of FeLV, recently, this decrease has reportedly stagnated in some countries. This study aimed to prospectively determine the prevalence of FeLV viraemia in cats taken to veterinary facilities in 32 European countries. FeLV viral RNA was semiquantitatively detected in saliva, using RT-qPCR as a measure of viraemia. Risk and protective factors were assessed using an online questionnaire to report geographic, demographic, husbandry, FeLV vaccination, and clinical data. The overall prevalence of FeLV viraemia in cats visiting a veterinary facility, of which 10.4% were shelter and rescue cats, was 2.3% (141/6005; 95% CI: 2.0%–2.8%) with the highest prevalences in Portugal, Hungary, and Italy/Malta (5.7%–8.8%). Using multivariate analysis, seven risk factors (Southern Europe, male intact, 1–6 years of age, indoor and outdoor or outdoor-only living, living in a group of ≥5 cats, illness), and three protective factors (Northern Europe, Western Europe, pedigree cats) were identified. Using classification and regression tree (CART) analysis, the origin of cats in Europe, pedigree, and access to outdoors were important predictors of FeLV status. FeLV-infected sick cats shed more viral RNA than FeLV-infected healthy cats, and they suffered more frequently from anaemia, anorexia, and gingivitis/stomatitis than uninfected sick cats. Most cats had never been FeLV-vaccinated; vaccination rates were indirectly associated with the gross domestic product (GDP) per capita. In conclusion, we identified countries where FeLV was undetectable, demonstrating that the infection can be eradicated and highlighting those regions where awareness and prevention should be increased.

The effect of a hiding box on stress levels and body weight in Dutch shelter cats; a randomized controlled trial.

While staying in an animal shelter, cats may suffer from chronic stress which impairs their health and welfare. Providing opportunities to hide can significantly reduce behavioural stress in cats, but confirmation with physical parameters is needed. Therefore, the aim of this study was to determine the effect of a hiding box on behavioural stress levels (scored by means of the Cat-Stress-Score) and a physical parameter, namely body weight, during the first 12 days in quarantine for cats newly arrived cats at a Dutch animal shelter. Twenty-three cats between 1 and 10 years of age were randomly divided between the experimental (N = 12) and control group (N = 11) with and without a hiding box. Stress levels were assessed on days 1, 2, 3, 5, 7, 9 and 12 according to the non-invasive Cat-Stress-Score (CSS). Body weights were measured on days 0, 7 and 12. Finally, adoption rates and length of stay (LOS) were determined. Major findings of the study are: (1) the mean Cat-Stress-Score decreased with time for all cats, but cats with a hiding box showed a significant faster decrease in the CSS, reaching a lower CSS-steady state seven days earlier than the control group; (2) nearly all cats in both groups lost significant body weight during the first two weeks; (3) hiding boxes did not significantly influence weight loss; (4) no differences were found in the adoption rates and the LOS between both groups. Hiding enrichment reduces behavioural stress in shelter cats during quarantine situations and can therefore be a relatively simple aid to shelter adaptation. It offers no prevention however against feline weight loss, which indicates a serious health risk for shelter cats.

Shelters Reflect but Cannot Solve Underlying Problems with Relinquished and Stray Animals-A Retrospective Study of Dogs and Cats Entering and Leaving Shelters in Denmark from 2004 to 2017.

Simple SummaryLarge numbers of dogs and cats end up as stray and homeless animals, and they are often viewed as a problem. Animal protection non-governmental organizations (NGOs), particularly in the Western world, have established shelters to deal with this issue. Their ideal goal is to rehome stray and abandoned dogs and cats to avoid them living difficult lives as strays or being euthanised. The current study assessed the number of cats and dogs entering and leaving shelters in Denmark over a 14-year period and aimed to determine how well this goal has been achieved. Denmark is interesting because there are no stray dogs, while cats to a large extent roam freely. In the case of dogs, there was a relatively low and slight decreasing intake and a low euthanasia rate during the period. From the start there was a higher intake and a higher euthanasia rate for cats, and during the period shelters had a 250% increase in the intake of cats. Although a larger number of cats were adopted, the proportion of shelter cats that were euthanised increased from 15% to 29%. Increased shelter capacity by itself cannot solve the problem with stray cats.Data covering about 90% of the estimated intake of dogs and cats to Danish shelters from 2004 to 2017 were used to study the effects of tight control of dogs and of efforts to increase shelter services for unwanted or stray cats. During the period, there was a low and decreasing intake of dogs, while the annual proportion of euthanised dogs increased from 6% to 10%. The number of cats entering shelters increased by about 250%, while the annual proportion of euthanised cats increased from 15% to about 29%. At the same time, there seemed to be a decrease in the population of stray cats. The major increase in cat intake may be due to animal protection non-governmental organizations (NGOs) making it easier to relinquish cats into shelters. Dog shelters can successfully handle surplus animals because dogs are well controlled by owners and are tightly regulated. Cats are more difficult to confine, are often allowed to roam freely and are less regulated. Therefore, cat shelters cannot solve the problem of surplus cats on their own. It is argued that an economic analysis may serve as a point of departure for a discussion on better policy making for NGOs in charge of shelters.

Phage constructs targeting gonadotropin-releasing hormone for fertility control: evaluation in cats.

Phage-gonadotropin-releasing hormone (GnRH) constructs with potential contraceptive properties were generated in our previous study via selection from a phage display library using neutralizing GnRH antibodies as selection targets. In mice, these constructs invoked the production of antibodies against GnRH and suppressed serum testosterone. The goal of this study was to evaluate this vaccine against GnRH for its potential to suppress reproductive characteristics in cats. Sexually mature male cats were injected with a phage-GnRH vaccine using the following treatment groups: (1) single phage-GnRH vaccine with adjuvant; (2) phage-GnRH vaccine without adjuvant and half-dose booster 1 month later; or (3) phage-GnRH vaccine with adjuvant and two half-dose boosters with adjuvant 3 and 6 months later. Anti-GnRH antibodies and serum testosterone, testicular volume and sperm characteristics were evaluated monthly for 7-9 months. All cats developed anti-GnRH antibodies following immunization. Serum antibody titers increased significantly after booster immunizations. In group 3, serum testosterone was suppressed 8 months after primary immunization. Total testicular volume decreased in group 1 by 24-42% and in group 3 by 15-36% at 7 months after immunization, indicating potential gonadal atrophy. Vacuolation of epididymides was observed histologically. Although all cats produced sperm at the conclusion of the study, normal morphology was decreased as much as 38%. Phage alone produced no local or systemic reactions. Immunization of phage with AdjuVac produced unacceptable injection site reactions. Our phage-based vaccine against GnRH demonstrated a potential for fertility impairment in cats. Future research is required to optimize vaccine regimens and identify animal age groups most responsive to the vaccine. If permanent contraception (highly desirable in feral and shelter cats) cannot be achieved, the vaccine has a potential use in zoo animals or pets where multiple administrations are more practical and/or reversible infertility is desirable.

Prevalence of Gastrointestinal Parasite on Cats in Shelter East Surabaya

The purpose of this study was to determine the prevalence of gastrointesatinal parasites in cats at Surabaya shelter. Eighty two samples of cat feces used in this study were taken from Griya satwa shelter and Kiro cat shelter. Feces samples were examined using the native, sedimentation and floating methods. Feces samples gastrointestinal parasite were identified by using a microscope with 400x and 1000x magnifications. Faces samples that are diagnosed positively if there is at least one of the three methods. Feces samples has observed, obtained prevalence rate of 43.9% or 36 positive samples from eighty two samples. The results of chi square analysis showed that p &gt; 0.05, which showed that the parasites taken in the two shelters had clear differences in the prevalence of the gastrointestinal tract parasites. The parasites observed were Isospora sp., Ancylostoma sp., Toxocara cati and Toxocara leonina.

Comparison of Outcome Data for Shelter Dogs and Cats in the Czech Republic.

Simple SummaryKnowing the factors that influence animal adoption is a key step towards making changes in shelters-the goal is to increase the adoption rates and to reduce the time spent by animals in shelters. The aim of the study was to compare the length of stay of dogs and cats in the selected shelters in the Czech Republic. A significantly different length of stay of dogs and cats in the shelters before adoption was found. Cats seem to have a worse chance of being adopted in comparison with shelter dogs.Animal protection, which also comprises the subject of abandoned and stray animals, has become a pressing and widely discussed topic. The aim of this study was to compare dogs and cats from two shelters in a selected region of the Czech Republic, based on factors that affect the length of stay. The following factors were analyzed: outcome, sex, age, and purebred status. A total of 419 cats and 2580 dogs housed in the monitored shelters from 2013 to 2016 were included in the analysis. The results show that dogs (n = 1343; 52.1%) are returned to their owners significantly more often (p < 0.001) than cats (n = 10; 2.4%). Dogs stayed in the shelter significantly (p < 0.001) less time than cats regardless of the outcome (the median length of stay of dogs were 3 days, while that of cats was 51 days). Also the length of stay in the shelter until adoption is shorter in dogs than in cats (dogs: median 27 days; cats: median 53 days). Median length of stay tended to increase with the increasing age in both species. Monitored age categories of dogs and cats differed significantly (p < 0.05) in their median length of stay (LOS) until adoption. We found that purebred status does not affect the length of stay in the shelter until adoption, either in dogs or in cats. Overall, our results suggest that dogs are preferred over cats in the Czech Republic. Shelter operators should take into account this aspect affecting animal adoption. By targeted efforts and education of public, it is possible to mitigate the negative effects of favoring a certain category of animals over others.

Molecular detection and characterisation of feline morbillivirus in domestic cats in Malaysia

Feline morbillivirus (FeMV), a novel virus from the family of Paramyxoviridae, was first identified in stray cat populations. The objectives of the current study were to (i) determine the molecular prevalence of FeMV in Malaysia; (ii) identify risk factors associated with FeMV infection; and (iii) characterise any FeMV isolates by phylogenetic analyses. Molecular analysis utilising nested RT-PCR assay targeting the L gene of FeMV performed on either urine, blood and/or kidney samples collected from 208 cats in this study revealed 82 (39.4%) positive cats. FeMV-positive samples were obtained from 63/124 (50.8%) urine and 20/25 (80.0%) kidneys while all blood samples were negative for FeMV. In addition, from the 35 cats that had more than one type of samples collected (blood and urine; blood and kidney; blood, urine and kidney), only one cat had FeMV RNA in the urine and kidney samples. Risk factors such as gender, presence of kidney-associated symptoms and cat source were also investigated. Male cats had a higher risk (p = 0.031) of FeMV infection than females. In addition, no significant association (p = 0.083) was observed between the presence of kidney-associated symptoms with FeMV status. From the 82 positive samples, FeMV RNA was detected from 48/82 (58.5%) pet cats and 34/126 (27.0%) shelter cats (p < 0.0001). Partial L and N gene sequencing of the RT-PCR-positive samples showed 85–99% identity to the published FeMV sequences and it was significantly different from all other morbilliviruses. A phylogenetic analysis of the identified Malaysian FeMVs was performed with isolates from Japan, Thailand and China. Molecular characterisation revealed high relatedness of the Malaysian isolates with other Asian FeMVs, indicating that the virus had been circulating only within the region. Therefore, this study confirmed the existence of FeMV among domestic cats in Malaysia. The findings suggest further characterisation of the local isolates, including the whole genome sequencing and that studies at determining the direct consequences of FeMV infection in domestic cats are needed.

Prevalence of endoparasites in northern Mississippi shelter cats.

Parasitism of domestic cats impacts feline health and public health, when zoonotic parasites are present. Our objective was to evaluate endoparasite prevalence in cats from northern Mississippi animal shelters. Feline cadavers (n = 56) were collected from seven shelters from August 2017 to January 2018. Data included shelter, sex, reproductive status, intake date, originating source, and treatment records. Cadavers were processed to isolate stomach, and small and large intestines. Contents were strained and examined using stereomicroscopes for helminth collection and identification. Centrifugal flotation using Sheather's solution was performed on feces; urine sediments were also examined. Descriptive statistics in SAS was performed using the Frequency procedure. Kappa agreement statistics were obtained to determine agreement between fecal flotation and necropsy results. Separate logistic regression models were developed to test effects of risk factors on the probability for cats to test positive for outcomes of interest. Helminths were recovered in 82% of cats (46/56); specifically, Ancylostoma spp. (52%), Toxocara cati (43%), Taenia taeniaeformis (36%), Dipylidium caninum (29%), and Spirometra spp. (4%) were identified. Thirty-seven of 56 cats (66%) had parasite eggs or oocysts on fecal examination, including T. cati (39%), Ancylostoma spp. (34%), Cystoisospora spp. (23%), Spirometra spp. (9%), T. taeniaeformis (9%), and capillarid-type eggs (5%). Feline originating source was associated with presence of T. cati eggs in feces and presence of D. caninum in the gastrointestinal tract. Feral cats were more likely to have T. cati eggs in feces than owner surrender cats (OR 28; 95% CI: 1.9, 423), or stray cats (OR 8, 95% CI: 1.1, 57.0). Owner surrender cats were more likely to have D. caninum helminths in the gastrointestinal tract than stray cats (OR = 19.5; 95% CI: 2.0, 190). Toxocara cati exhibited strong agreement (κ = 0.70, 95% CI: 0.52, 0.89), Ancylostoma spp. exhibited moderate agreement (κ = 0.44, 95% CI: 0.22, 0.65), and cestodes exhibited poor agreement (κ = 0.02, 95% CI: -0.12, 0.15) between presence of eggs and gross helminths. Capillarid eggs (Pearsonema feliscati) were recovered in urine sediment of 6% (3/48) of cats. Overall, our study demonstrates a high level of parasitism in cats that entered Mississippi animal shelters. Parasites with zoonotic potential, such as Alaria spp., Ancylostoma spp., D. caninum, Physaloptera spp., T. taeniaeformis, T. cati, and Spirometra spp. were identified. Our results support the need for effective antiparasitic treatment of cats entering animal shelters in order to improve feline health and prevent environmental contamination with zoonotic parasites.

Gastrointestinal parasites in shelter cats of central Italy.

In Italy, the limited resources and the large number of cats living in feline shelters may facilitate the spread of parasite infections that may affect the health of cats and expose shelter workers and adoptive owners to zoonosis. Prevalence and risk factors of potential zoonotic and host-specific gastrointestinal parasite infections were assessed in cats living in public and private shelters of central Italy. Individual fecal samples were examined macroscopically and then screened microscopically by fresh and Lugol stained fecal smears and by flotation and the Baermann tests. A commercial rapid immune-chromatographic assay was used to detect Giardia duodenalis and Cryptosporidium spp. All animals were clinically examined to evaluate presence of clinical signs compatible to parasitic infections. Data were statistically analysed by multivariate analysis with logistic regression and chi square testing for bivariate analysis. The overall prevalence of gastrointestinal parasites in the shelter cats surveyed was 22% (29/132), with 26/132 (19.7%) of the animals infected with potentially zoonotic organisms. Upon statistical analysis, parasite infections were identified in significantly more cats younger than 18 months of age (p <0.05), and most often associated with the presence of compatible clinical signs (p <0.05). A higher prevalence of protozoan infections (18.1%, 24/132) than of helminth infections (12.9%, 17/132) was observed. Identified parasites were Giardia duodenalis (10.6%, 14/132), Toxocara cati (9%, 12/132), Cystoisospora felis (3%, 4/132), Cystoisospora rivolta (2.3%, 3/132), hookworms (2.3%, 3/132), Cryptosporidium spp. (1.6%, 2/132), Aonchotheca putorii (0.75%, 1/132), Tritrichomonas foetus (0.75%, 1/132) and Strongyloides sp. (0.75%, 1/132). Co-infections were identified in the 7.6% of animals surveyed. Results obtained from this study indicate high gastrointestinal parasite risk to shelter cats and public health in the examined areas and suggest the need for more effective control measures in shelters surveyed.

Oral lesions and retroviruses in shelter cats

ABSTRACT: Oral lesions are common problems in feline medicine worldwide, and may be associated with different causes, such as infectious agents. There are only a few studies reporting the chief oral diseases and the results for retrovirus tests in shelter cats in Brazil, especially in the South region. This study aimed to identify the main inflammatory oral lesions in shelter cats and verify the test results for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) infections. Forty-three felines from private shelters in the central region of Rio Grande do Sul state (RS) that presented clinically evident oral lesions, regardless of age, breed, sex, and neuter status, were used in this survey. Serological tests for FIV and FeLV were performed in all cats, and data regarding the rearing system were collected. Sixteen cats (37.2%) were reared in a free system, whereas 27 (62.8%) were kept under a restrict system. Of the 43 cats with oral lesions, 29 (67.44%) presented only one type of lesion, characterized as periodontitis (n=22, 51.16%), followed by gingivitis (n=6, 13.95%), and stomatitis (n=1, 2.32%). Concomitant stomatitis and periodontitis were found in the 14 remaining cats (100%). With respect to the test results for retrovirus infections, nine (20.93%) of the 43 felines were positive for FIV alone. Co-infection with both viruses was observed in seven cats (16.28%). No cat was seropositive for FeLV valone. None of the six cats that presented gingivitis was positive for FIV and FeLV; one cat with stomatitis was positive for FIV and FeLV; of the 22 cats with periodontitis, six (27.27%) were FIV positive and two (9.09%) were FIV/FeLV positive; and of the 14 cats that presented stomatitis and periodontitis, three (21.43%) were FIV positive and four (28.57%) were FIV/FeLV positive. As for diagnosis, 28 cats (65.1%) presented solely periodontal disease (PD), one cat (2.32%) had feline chronic gingivostomatitis (FCG) alone, and 14 (32.5%) had both PD and FCG. The results obtained show that the main oral lesions found in shelter cats in the central region of RS were gingivitis, stomatitis, and periodontitis. Periodontitis, in association or not with stomatitis, was the most frequently observed oral cavity lesion in FIV- and/or FeLV-positive cats. Other factors may contribute to installation of inflammatory oral diseases in shelter cats because most cats with oral cavity lesions tested negative for retrovirus infections.