Shell Production Research Articles

Meso-microporous activated carbon derived from Raffia palm shells: optimization of synthesis conditions using response surface methodology

This study investigated the optimal synthesis conditions for the production of Raffia Palm Shell Activated Carbon (RPSAC) using phosphoric acid as activation agent. The optimization of the synthesis conditions was achieved using the Central Composite Design (CDD) in Response Surface Methodology (RSM). The influences of impregnation ratio, temperature, time and concentration on the specific surface area and yield of RPSAC were evaluated. Based on the CDD, 2FI and quadratic models were developed for the two responses. Analysis of Variance (ANOVA) was utilized to determine the significant factors and factor interactions for each response. All process variables except impregnation ratio were observed to significantly influence the quality of RPSAC. The optimal synthesis conditions for RPSAC were; 523.68 °C, 76.91%, and 103.83 min for temperature, concentration, and time respectively which provided a specific surface area and yield of 1762.92 m2/g and 77.98 % respectively. The Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray (EDX) analyses proved that RPSAC had a meso-micro-porous morphology with high carbon and oxygen contents. Fourier-transform infrared spectroscopy (FTIR) revealed the abundance of hydroxyl, carbonyl and carboxylic groups on RPSAC. X-ray Powder Diffraction (XRD) analysis showed that RPSAC composed mainly of amorphous and disordered microcrystalline phases ascribed to the high quartz content of the precursor. The Brunauer–Emmett–Teller (BET) surface area, average pore diameter, total pore volume, and pHpzc of RPSAC were obtained as 456.10 m2/g, 0.25 cm3/g, 2.13 nm and 2.10 correspondingly. Thus, RSM was found to be an excellent and desirable tool for optimal synthesis of RPSAC that possess high surface area and porosity suitable for application in the adsorption of both large and small molecular sized pollutants such as dyes and fluoride in real and aqueous solution.

A Bivalve Biomineralization Toolbox.

Mollusc shells are a result of the deposition of crystalline and amorphous calcite catalyzed by enzymes and shell matrix proteins (SMP). Developing a detailed understanding of bivalve mollusc biomineralization pathways is complicated not only by the multiplicity of shell forms and microstructures in this class, but also by the evolution of associated proteins by domain co-option and domain shuffling. In spite of this, a minimal biomineralization toolbox comprising proteins and protein domains critical for shell production across species has been identified. Using a matched pair design to reduce experimental noise from inter-individual variation, combined with damage-repair experiments and a database of biomineralization SMPs derived from published works, proteins were identified that are likely to be involved in shell calcification. Eighteen new, shared proteins likely to be involved in the processes related to the calcification of shells were identified by the analysis of genes expressed during repair in Crassostrea gigas, Mytilus edulis, and Pecten maximus. Genes involved in ion transport were also identified as potentially involved in calcification either via the maintenance of cell acid–base balance or transport of critical ions to the extrapallial space, the site of shell assembly. These data expand the number of candidate biomineralization proteins in bivalve molluscs for future functional studies and define a minimal functional protein domain set required to produce solid microstructures from soluble calcium carbonate. This is important for understanding molluscan shell evolution, the likely impacts of environmental change on biomineralization processes, materials science, and biomimicry research.

Liquid crystal elastomer shells with topological defect-defined actuation: Complex shape morphing, opening/closing, and unidirectional rotation

We produce hollow sphere liquid crystal elastomer (LCE) actuators from a nematic precursor mixture, brought into the shape of a self-closing shell with tangential anchoring of the director field n(r), using a solvent-assisted microfluidic technique. By separating the shell production from the polymerization and cross-linking, the precursor is allowed to approach its equilibrium n(r) configuration in the shell, spontaneously forming topological defects of total strength +2. However, the photopolymerization into an LCE induces a brief but strong distortion of the overall n(r) and the defect configuration, even changing the ground state shape in the case of thick shells. The resulting LCE shells show a rich capacity for reversible shape morphing upon heating and cooling, the exact actuation mode defined by n(r), and the final defect configuration stabilized at the end of polymerization. In regions with a single +1 defect, a reversal of curvature from concave to convex is found, punctured shells exhibit a strong shape change between a nearly closed sphere at low temperature and an open-ended spherocylinder at high temperature, and all shells rotate upon actuation when suspended in a fluid. As the rotation is stronger during relaxation than during actuation, thus breaking the symmetry, the net rotation is unidirectional.

Development of Condition Monitoring System for Electric Resistance Spot Welding Used to Manufacture Railway Car Bodies

Stainless steel railway car bodies are assembled by joining the outer plates and the pillar materials using resistance spot welding. In recent years, more and more car bodies are being assembled using laser welding in addition to the resistance spot welding. For this laser welding system, we developed a condition monitoring system considering the processes before and after laser welding as a single system, and obtained and put into practical use an appropriate condition that suppresses spatter generation during laser welding. On the other hand, in resistance spot welding, the current, weld time, electrode load, and electrode tip shape are the main factors that determine the welding quality. Therefore, the configuration of the equipment is less complicated than that of laser welding system, and the condition monitoring is easier than that of the laser welding. In this study, by transferring the concept of the condition monitoring system developed for laser welding to resistance spot welding, we achieved a reduction of more than 60% in terms of electricity consumption and improved the appearance of the car body by optimizing the indentation shape. In addition to this technical achievement, we also present in this paper a case study showing the opportunity for innovation by restructuring the technological paradigm of the resistance spot welding in the production of stainless steel car body shells.

Effects of extra feeding combined with ocean acidification and increased temperature on the carbon isotope values (δ13C) in the mussel shell

Ocean acidification (OA) and global warming present future challenges for shell producing organisms such as mussels through reduction in the carbonate available to produce shells in these and other valuable aquaculture species. Molluscs control their shell growth through biomineralisation, but the response of the mechanisms behind biomineralisation to OA conditions are relatively unknown. It is unclear how much carbon is taken into the shell from the environment compared to the uptake through the food source. Shell production is energetically costly to molluscs and metabolic processes and energetic partitioning may affect their ability to perform the underlying mechanisms of biomineralisation under OA. It is possible that additional food consumption might alleviate some impacts caused by acidification. We assessed the ability of extra feeding to alter the impacts of OA and increased temperatures on adult Mytilus edulis. Carbon isotopes (δ13C) were used to examine the change in biomineralisation pathway in mussels. OA did not alter the δ13C directly in separate analyses of the shell calcite and aragonite layers, mantle tissue and extrapallial fluid. However, ambient treatments with increased temperatures altered the mussel biomineralisation pathway in the shell calcite using CO32− instead of HCO3− as the main source of carbon. The proportion of metabolic carbon uptake into the mussel shell calcite layer increased under OA, with additive effects when exposed to increased temperatures and extra feeding. The proportion of metabolic carbon uptake is higher (7%–11%) in the shell aragonite layer compared to calcite, under ambient treatments. OA initially reduced the metabolic carbon uptake into the shell aragonite, but after a period of 4-months with extra feeding, the mussels were able to adjust their metabolic carbon uptake to a level experienced under ambient treatments. This indicates that an abundance of food resources may enable changes in mussel biomineralisation pathways to compensate for any decrease in seawater inorganic carbon associated with OA. The impact of OA on phytoplankton varies from species to species, changing the structure of the community which could provide sufficient food resources to maintain metabolic carbon uptake for mussel shell growth. This study of δ13C isotopic values has identified changes in biomineralisation pathway relating to the mussel metabolic carbon uptake from their food source, with varying results for the aragonite and calcite shell polymorphs. The implications of these findings suggest that some bivalve species with different shell composites may cope better under OA than others, demanding further study into species-specific biomineralisation pathways.

Improvement of the technology of functional pre-made meat products in a dough shell

In modern conditions of increasing globalization and intensifying competition in the food industry in general and in the meat processing industry in particular, there is a need to find new ways to increase the competitiveness of enterprises, guarantee the quality and safety of products, ensure proper working conditions for personnel, and reduce the negative impact on the environment. The research aims to improve the technology of pre-made products in dough shell production and to determine the rational parameters of the production process by introducing new ingredients into the recipe. The content of toxic elements was determined based on the Ukrainian Laboratory of Quality and Safety of Agricultural Products of the National University of Life and Environmental Sciences of Ukraine. The main stages of production of pre-made products in dough shells were analyzed, the technology was improved considering a set of safety and quality studies, risks and critical control points, sources of their occurrence were identified, and preventive actions were developed. The amino acid composition of the protein component and functional and technological quality indicators of the minced meat samples (moisture retention capacity, emulsifying capacity, and stability of meat emulsions) were evaluated. To determine the changes that occurred in the experimental prepared products enriched with vegetable components, a study of changes in their physicochemical properties after freezing and thawing was conducted. The research results showed that in the experimental sample containing the food additive Elamin, the amount of bound moisture in meat systems gradually decreases during storage, but the mass fraction of the concentrate in the amount of 0.3 kg per 100 kg of raw material allows for retaining free moisture. Based on the research results, the technology of functional pre-made meat products in a dough shell was improved, and technical specifications and technological instructions state standard 10.1-00493706-075:2019 “Frozen semi-finished products in a dough shell “Healthy dumplings” were developed for implementation in production

The early Cenomanian crippsi Event at L\xfcneburg (Germany): palaeontological and stratigraphical significance of a widespread Late Cretaceous bioevent

The early Cenomanian crippsi Event comprises a 1–3-m-thick interval characterised by mass occurrences of the early Cenomanian inoceramid Gnesioceramus crippsi, identified in the uppermost Sharpeiceras schlueteri Subzone (lower lower Cenomanian Mantelliceras mantelli Zone), below an interregional sequence boundary (SB Ce 1). At Lüneburg, the event is characterised by densely packed, very large, disc-like valves of G. crippsi. Taphonomy as well as bio- and microfacies suggest an event formation in a deeper shelf setting below the storm-wave base as primary biogenic concentration, the inoceramids living as recumbent forms on a soft substrate in dense populations. When tracked between basins, the stratigraphic pattern of the crippsi Event suggests a moderately prolonged phase (< 100 kyr) of increased shell production with rapid deposition aiding in preserving the shell-rich event strata. Towards the basin margins, it grades into storm wave-reworked bioclastic concentrations. The crippsi Event formed by an interregional population bloom and provides, as an proliferation epibole, an important marker for intra- and interbasinal correlation. The first record of G. mowriensis within the crippsi Event at Lüneburg, hitherto endemic to the US Western Interior Seaway, and the occurrence of the ammonite Metengonoceras teigenense, likewise an endemic North American faunal element, from the level of the crippsi Event in northern France indicate faunal exchange between the New and Old worlds during the early Cenomanian. This faunal dispersal and contemporaneous occurrence of warm-water biofacies in Western Europe during the early Cenomanian is explained by the existence of a perpetual NE-directed current transporting warm surface waters from the Gulf of Mexico towards Europe. The occurrence of short-lived M. teigenense in France allows for the calibration of the uppermost schlueteri Subzone of the mantelli Zone in Europe to the lowermost Neogastroplites muelleri Zone in North America and to assign an age of ~ 98.6–98.7 Ma to the crippsi Event.

Scalable Production of Monolayer Shell(Pt)@Core(Pd) Nanoparticles by Electroless Cu UPD for Oxygen Reduction Reaction

In this article, we discuss an electroless under potential deposition (UPD) method to synthesize core@shell monolayer nanoparticles in bulk quantities. In this electroless UPD method, potential is controlled and maintained in the UPD region by a redox couple. This electroless path enables the production of core@shell monolayer catalysts on any type of support, unlike the conventional UPD, where potential is controlled by an external power source which allows for the deposition only on conductive supports such as carbon. This was demonstrated by synthesizing Pt(shell)@Pd(core) nanopartricles on both conductive (Vulcan carbon) and non-conductive (alumina) supports by Cu UPD-galvanic replacement method. Core-shell structure of the Pd-Pt nanoparticles was confirmed by STEM characterization. The thickness of Pt shell in Pt@Pd nanoparticles was examined by analytical and experimental observations. Furthermore, catalytic activity of Pt@Pd/C nanoparticles, synthesized by the electroless Cu UPD-galvanic replacement method, was examined for oxygen reduction reaction.

Production of bottom flange shells by cold extrusion on specialized press

The possibility for manufacturing by cold extrusion of part such as shells made of corrosion-resistant and higstemperature steel is evaluated. It is shown that when extrusion such parts using combination of direct extrusion from workpiece with diameter equal to the diameter of the flange, followed by direct extrusion of the shell wall, number of intractable problems arise. Some of these problems relate to the design of production die to be fitted to mass produced standard presses. It is proposed to manufacture parts of the type using combination of extrusion of the shell walls from workpiece with diameter equal to its outer diameter, with simultaneous radial extrusion of the flange. Such technological solution can be implemented on domestic specialized presses.

Производство боеприпасов на заводе «Красное Сормово» в 1941–1944 гг.

The article discusses the production of ammunition at the Krasnoye Sormovo plant during the Second World War. Based on the study of general scientific and specialized archival materials, systematization and generalization of the data obtained, an analysis of the activities of the labor collective and management structures of the Krasnoye Sormovo plant for the production of ammunition is carried out. The dynamics of the growth in the production of shells and the development of new types of ammunition in the period 19411944 is determined, the process of creating mass production of shells in the workshops of the plant, strengthening cooperation with other defense enterprises and organizing railway communication between them is shown. The methods of modernization and improvement of equipment and technology for the production of ammunition, methods of increasing the production and personnel potential of the plant, as well as the heroic work of the factory workers in wartime conditions are examined in detail. During the war period, the share of the production of ammunition in the total volume was about 17%, which objectively testifies to the contribution of the production of artillery and rockets at the Krasnoye Sormovo plant to a radical turning point during the Second World War.

Factors affecting egg consumption in young consumers

Factors affecting egg consumption in young consumers

A century of coping with environmental and ecological changes via compensatory biomineralization in mussels.

Accurate biological models are critical to predict biotic responses to climate change and human‐caused disturbances. Current understanding of organismal responses to change stems from studies over relatively short timescales. However, most projections lack long‐term observations incorporating the potential for transgenerational phenotypic plasticity and genetic adaption, the keys to resistance. Here, we describe unexpected temporal compensatory responses in biomineralization as a mechanism for resistance to altered environmental conditions and predation impacts in a calcifying foundation species. We evaluated exceptional archival specimens of the blue mussel Mytilus edulis collected regularly between 1904 and 2016 along 15 km of Belgian coastline, along with records of key environmental descriptors and predators. Contrary to global‐scale predictions, shell production increased over the last century, highlighting a protective capacity of mussels for qualitative and quantitative trade‐offs in biomineralization as compensatory responses to altered environments. We also demonstrated the role of changes in predator communities in stimulating unanticipated biological trends that run contrary to experimental predictive models under future climate scenarios. Analysis of archival records has a key role for anticipating emergent impacts of climate change.

On the High Fluid Pressure in Hydrostatic Forming for Sheet Metal

In hydrostatic forming, technological parameters have a significant influence on the thin shell product formation. One of the most important factors is the forming liquid pressure. This pressure exerts directly on the workpiece, pulling the workpiece closely to the die's profile. Therefore, this parameter should be kept large enough for shaping the product with the required shape and size. In practice, however, it is very difficult to achieve and maintain the high value of this parameter. In addition, it is also necessary to set up a mathematical model of this parameter to support the calculation and control. This paper has suggested a simple solution for maintaining fluid pressure during forming. The solution has been applied into the experimental system. Using this system to investigate forming liquid pressure parameter, the paper has given a suitable mathematical model when shaping cylindrical details from sheet metal. The results of the study contribute to data in die design, parameter calculation and control as well as forming process stabilization in hydrostatic forming for sheet metal.

Steel making cities of labor prowess: historical memory and new opportunities

In the beginning of 2020 in Russia an honorary title “Сity of labor prowess” was established, which was awarded to the cities, citizens of which made a significant contribution to reaching the Victory of the USSR in the Great Patriotic War of 1941– 1945. The cities to receive the new title ensured an uninterrupted production of war and civil products at plants during the war time and the citizens showed mass labor heroism and selflessness, confirmed by awarding of plants and workers by state rewards as well as presentation of challenge Red Flags of State Defense Committee. On July 2, 2020 the new title was awarded to 20 cities, among which were many centers of steel industry, in particular, Magnitogorsk, Chelyabinsk, Nizhny Tagil, Novosibirsk, Izhevsk, Novokuznetsk. Steel industry in war years provided the needs of the country and the Red Army by all kinds of metals. Steel plants within a short time managed to arrange a wide-scale production of armor plates, gun, shell, armor-piercer steels and other new for them high quality alloyed steels , necessary for production of armament and war machinery. Besides, at the steel plants a production of ammunition and utilities for weapon and war machinery was mastered. The title “City of labor prowess” is a “civil” analogue of the title “City of military velour”. Despite this status does not envisages any material stimulation of citizens and additional financing of a city, receiving of it will enable not only to preserve the historical memory and to show respect to heroic forefathers, but also give a new pulse to development of regions, as well as will attract attention of mass media, business and federal authorities and will increase the tourist attractiveness.

Modern middens: Shell recycling for restoring an endangered marine ecosystem in Victoria, Australia

SummaryShellfish reefs are one of the most depleted marine ecosystems in the world, and there is considerable interest and efforts to restore the ecosystems. When degraded reefs require the addition of new substrate to support oyster re‐establishment, recycled shells can be used to rebuild new reef bases. Shell recycling as a means of procuring shells was first introduced in Australia by The Nature Conservancy in 2015, through the establishment of the ‘Shuck Don’t Chuck’ Shell Recycling Project in Victoria. The recycling project was first piloted in the regional city of Geelong with the aim of providing sufficient quantity of clean, recycled shell to support reef base construction for shellfish reef restoration in Port Phillip Bay. The project has subsequently expanded into markets and restaurants in Melbourne. Community involvement and restoration promotion are important secondary outcomes of the programme. We provide lessons from our experience to date and a step‐by‐step guide of how to establish a shell recycling project from initial pilot and trial stages to a full programme. These steps include the following: (i) recruitment of shell recycling partners, (ii) transport and logistics, (iii) shell storage sites, (iv) biosecurity and curing protocols, (v) hatchery use, (vi) reef deployment, and (vii) community engagement. Shell recycling is an effective way to not only source substrate for restoring shellfish reefs, but also to reduce the amount of waste going to landfill and should be considered as part of any shellfish reef restoration project which utilises shell products.

A practical technique for hydrodynamic coefficients modification in SHEAR7 for fatigue assessment of riser buoyancy modules under vortex-induced vibration

The hydrodynamic coefficients in semi-empirical tool are optimized for a riser attached with staggered buoyancy modules undergoing Vortex-Induced Vibration (VIV) based on the SHELL Oil Experiment. The existing prediction tools are based on data derived from experiments, number of assumptions from strip theory, energy balance between fluid and structure and use of lift coefficient databases. Current advancements in conducting and calculating VIV response from the experiments shows that these assumptions may be invalid. The major difference arises between experimental observations and theoretical estimations is from lift coefficient databases. The databases extracted under laboratory conditions have limited Reynolds number flow conditions and only cross-flow motions were considered. The extracted hydrodynamic excitation coefficient database is posed to optimization problem, where the main objective is to minimize the prediction error of semi-empirical tools when compared with experimental results. We optimize the existing excitation coefficient database to improve the fatigue damage prediction of riser attached with staggered buoyancy modules. Application of modification factors and optimizing the hydrodynamic lift coefficients to the data from SHELL Exploration and Production Test provides the new optimized excitation coefficient datasets which reduce the error in the predicting the VIV response of the riser with staggered buoyancy modules.

Experimental modeling of fluid pressure in hydrostatic forming for cylindrical product

Forming fluid pressure is an important technological parameter that determines whether a product can be accurately formed according to the size and profile of the die in hydrostatic forming technology. The expected value of this parameter is often very high because it acts as the punch in forming complex products from sheet metal. However, it is difficult to achieve high values because the forming fluid pressure depends on the ability to hold high pressure of equipment system and input parameters including the blank holder pressure, the depth of die, the thickness of workpiece. Moreover, it is also necessary to have a mathematical model for this parameter to facilitate the calculation and control of the forming process. In order to solve the above problems, this paper will indicate a simple way to avoid the pressure drop during forming process, and establish a regression function relationship between the forming fluid pressure for typical cylindrical product and input parameters above by experimental research method. The results contribute to die design, calculation and control of process parameters to facilitate shaping thin shell products in actual hydrostatic forming technology.

A path(-integral) toward non-perturbative effects in Hawking radiation

Hawking’s seminal discovery of black hole evaporation was based on the semi-classical, perturbative method. Whether black hole evaporation may result in the loss of information remains undetermined. The solution to this paradox would most likely rely on the knowledge of the end-life of the evaporation, which evidently must be in the non-perturbative regime. Here we reinterpret the Hawking radiation as the tunneling of instantons, which is inherently non-perturbative. For definitiveness, we invoke the picture of shell–anti-shell pair production and show that it is equivalent to that of instanton tunneling. We find that such a shell pair production picture can help to elucidate firewalls and [Formula: see text] conjectures that attempt to solve the information paradox, and may be able to address the end-life issue toward an ultimate resolution.

Deciphering mollusc shell production: the roles of genetic mechanisms through to ecology, aquaculture and biomimetics.

Most molluscs possess shells, constructed from a vast array of microstructures and architectures. The fully formed shell is composed of calcite or aragonite. These CaCO3 crystals form complex biocomposites with proteins, which although typically less than 5% of total shell mass, play significant roles in determining shell microstructure. Despite much research effort, large knowledge gaps remain in how molluscs construct and maintain their shells, and how they produce such a great diversity of forms. Here we synthesize results on how shell shape, microstructure, composition and organic content vary among, and within, species in response to numerous biotic and abiotic factors. At the local level, temperature, food supply and predation cues significantly affect shell morphology, whilst salinity has a much stronger influence across latitudes. Moreover, we emphasize how advances in genomic technologies [e.g. restriction site-associated DNA sequencing (RAD-Seq) and epigenetics] allow detailed examinations of whether morphological changes result from phenotypic plasticity or genetic adaptation, or a combination of these. RAD-Seq has already identified single nucleotide polymorphisms associated with temperature and aquaculture practices, whilst epigenetic processes have been shown significantly to modify shell construction to local conditions in, for example, Antarctica and New Zealand. We also synthesize results on the costs of shell construction and explore how these affect energetic trade-offs in animal metabolism. The cellular costs are still debated, with CaCO3 precipitation estimates ranging from 1-2 J/mg to 17-55 J/mg depending on experimental and environmental conditions. However, organic components are more expensive (~29 J/mg) and recent data indicate transmembrane calcium ion transporters can involve considerable costs. This review emphasizes the role that molecular analyses have played in demonstrating multiple evolutionary origins of biomineralization genes. Although these are characterized by lineage-specific proteins and unique combinations of co-opted genes, a small set of protein domains have been identified as a conserved biomineralization tool box. We further highlight the use of sequence data sets in providing candidate genes for in situ localization and protein function studies. The former has elucidated gene expression modularity in mantle tissue, improving understanding of the diversity of shell morphology synthesis. RNA interference (RNAi) and clustered regularly interspersed short palindromic repeats - CRISPR-associated protein 9 (CRISPR-Cas9) experiments have provided proof of concept for use in the functional investigation of mollusc gene sequences, showing for example that Pif (aragonite-binding) protein plays a significant role in structured nacre crystal growth and that the Lsdia1 gene sets shell chirality in Lymnaea stagnalis. Much research has focused on the impacts of ocean acidification on molluscs. Initial studies were predominantly pessimistic for future molluscan biodiversity. However, more sophisticated experiments incorporating selective breeding and multiple generations are identifying subtle effects and that variability within mollusc genomes has potential for adaption to future conditions. Furthermore, we highlight recent historical studies based on museum collections that demonstrate a greater resilience of molluscs to climate change compared with experimental data. The future of mollusc research lies not solely with ecological investigations into biodiversity, and this review synthesizes knowledge across disciplines to understand biomineralization. It spans research ranging from evolution and development, through predictions of biodiversity prospects and future-proofing of aquaculture to identifying new biomimetic opportunities and societal benefits from recycling shell products.

Deciphering shell proteome within different Baltic populations of mytilid mussels illustrates important local variability and potential consequences in the context of changing marine conditions

Molluscs defend themselves against predation and environmental stressors through the possession of mineralized shells. Mussels are widely used to predict the effects of abiotic factors such as salinity and pH on marine calcifiers in the context of changing ocean conditions. Shell matrix proteins are part of the molecular control regulating the biomineralization processes underpinning shell production. Under changing environmental conditions, differential expression of these proteins leads to the phenotypic plasticity of shells seen in many mollusc species. Low salinity decreases the availability of calcium and inorganic carbon in seawater and consequently energetic constraints often lead to thin, small and fragile shells in Mytilid mussels inhabiting Baltic Sea. To understand how the modulation of shell matrix proteins alters biomineralization, we compared the shell proteomes of mussels living under full marine conditions in the North Sea to those living in the low saline Baltic Sea. Modulation of proteins comprising the Mytilus biomineralization tool kit is observed. These data showed a relative increase in chitin related proteins, decrease in SD-rich, GA-rich shell matrix proteins indicating that altered protein scaffolding and mineral nucleation lead to impaired shell microstructures influencing shell resistance in Baltic Mytilid mussels. Interestingly, proteins with immunity domains in the shell matrix are also found to be modulated. Shell traits such as periostracum thickness, organic content and fracture resistance qualitatively correlates with the modulation of SMPs in Mytilid mussels providing key insights into control of biomineralization at molecular level in the context of changing marine conditions.