This study presents a novel dual-scale finite element method to establish a microstructure-informed ductile fracture criterion for ferrite–martensite dual-phase (DP) steel. At the macroscale, an anisotropic plasticity model with rate-dependent hardening was employed to simulate the material's deformation history. Simultaneously, at the microscale, a dislocation density-based crystal plasticity model was utilized to simulate deformation within a representative volume element (RVE) of the dual-phase steel, constructed using tomography aided by a plasma-focused ion beam/electron backscatter diffraction system.The material properties of the ferrite and martensite phases were determined through X-ray diffraction (XRD) analysis and load-displacement measurements obtained via nanoindentation for each phase. The RVE simulation results were validated against experimentally measured mechanical properties and microstructural changes. The local deformation history at the fracture initiation site, extracted from the macroscale model, was used as boundary conditions for the microscale RVE simulation; sequential dual-scale approach. The models were applied to specimens with varying notch radii, generating different local stress triaxialities and accumulated shear strains at fracture onset. This process allowed the establishment of a ductile fracture criterion, which was further tested in a hole expansion experiment, demonstrating close alignment with experimental data.This sequential dual-scale analysis effectively predicts the deformation behavior of multiphase metallic materials by incorporating realistic microstructures while minimizing computational costs. Consequently, the proposed ductile fracture prediction technique offers a robust method with broad applicability across various metallic materials.