Shade Trees Research Articles

Functional groups of leaf phenology are key to build climate-resilience in cocoa agroforestry systems

Agroforestry has the potential to enhance climate change adaptation. While benefits from agroforestry systems consisting of cash crops and shade trees are usually attributed to the (shade) trees, the trees can also have negative impacts due to resource competition with crops. Our hypothesis is that leaf phenology and height of shade trees determine their seasonal effect on crops. We test this hypothesis by categorizing shade tree species into functional groups based on leaf phenology, shade tree canopy height and shade tree light (wet and dry season) interception as well as the effects. To this end, leaf phenology and the effects on microclimate (temperature, air humidity, intercepted photoactive radiation (PAR)), soil water, stomatal conductance and cocoa yield were monitored monthly during wet and dry seasons over a two-year period on smallholder cocoa plantations in the northern cocoa belt of Ghana. Seven leaf phenological groups were identified. In the wet season, highest buffering effect of microclimate was recorded under the trees brevi-deciduous before dry season. During dry season, high PAR and lowest reduction in soil moisture were observed under the trees in the group of completely deciduous during dry season. The evergreen groups also showed less reduction in soil water than the brevi-deciduous groups. In the wet season, shade tree effects on cocoa tree yields in their sub canopy compared to the respective control of outer canopy with full sun ranged from positive (+10 %) to negative (-15 %) for the deciduous groups, while yield reductions for the evergreen groups ranged from −20 % to −33 %. While there were negative yield impacts for all phenological groups in the dry season, the trees in completely deciduous during dry season group recorded least penalties (-12 %) and the trees with evergreen upper canopy the highest (-35 %). The function of shade trees in enhancing climate resilience is therefore strongly dependent on their leaf phenological characteristics. Our study demonstrates how the key trait leaf phenology can be applied to successful design of climate-resilient agroforestry systems.

Improving Yield and Quality of ‘Balady’ Mandarin Trees by Using Shading Techniques and Reflective Materials in Response to Climate Change Under Flood Irrigation Conditions

Considering climate change predictions, it is logical to anticipate detrimental effects on the mandarin tree, an essential citrus crop. Therefore, scientists should promptly focus on developing methods to enhance its resistance to climatic stress effects such as sunscald. This study assesses the strategies employed in ‘Balady’ mandarin trees when covered by shading nets of varying colors and percentages (white 50%, green 50% or 63%, black 50% or 63%), as well as the application of reflective materials (kaolin at 4% and CaCO3 at 3%) on the micro-climate of orchards, leaf, and fruit surface temperatures, fruit sunburn%, productivity, and fruit quality. The results indicated that shade nets effectively reduced temperature and enhanced humidity, especially during the period from June to September, when compared to open-field treatments. Black shade nets, particularly those with a shading level of 63%, demonstrated the most notable decrease in canopy temperature and an elevation in humidity, surpassing the performance of green and white shade nets. The present study found that shade nets and reflecting materials like kaolin and calcium carbonate significantly reduced fruit sunburn. Trees without shade had a sunburn rate of 8.74%, while those with shade treatments suffered no sunburn. Kaolin foliar spray at a concentration of 4% and calcium carbonate at a concentration of 3% reduced sunburn incidence to 3.64% and 7.32%, respectively. These treatments also reduced the intensity of sunburn. All treatments increased fruit yield % compared to the control and yield efficiency (kg/m2), especially the trees covered with white shade net of a 50% shading rate provided the highest values (43.70 and 40.17%) and (5.24 and 5.47 kg/m2) compared to other treatments in both seasons, respectively. Trees covered with a white shade net of a 50% shading rate, followed by a green shade net of 50% and a 63% shading rate, as well as a black shade net of 50% and a 63% shading rate, tended to improve the physical and chemical fruit properties. Therefore, it could be recommended that trees be covered with a white shade net of a 50% shading rate or a green shade net of a 50 and 63% shading rate in summer months due to its beneficial impact on mitigating fruit sunburn damage and enhancing the productivity and quality of ‘‘Balady’’ mandarin trees. Hence, shade nets can be a beneficial technology to protect citrus fruits from sunburn without affecting fruit quality in commercial citrus farms.

Maize crop performance on different Agricultural Production Systems and co-inoculation with A. brasilense and T. asperellum

Considering the increasing global population and demand for food, it is crucial to adopt agricultural practices that ensure both food security and environmental preservation. In this context, plant growth-promoting microorganisms (PGPM) and integrated crop-livestock-forestry systems (ICLF) have emerged as promising strategies for sustainable productive intensification. This research aimed to determine the effects of inoculating Azospirillum brasilense and Trichoderma asperellum on maize crops (Zea mays L.) grown in monocropping and ICLF systems. A complete randomized block design was performed in a 4 × 2 factorial arrangement with three replications. The crop systems included maize monocrop (C), crop-livestock (CL), crop-forest (CF), and crop-livestock-forest (CLF). Concomitantly, the co-inoculation (A. brasilense + T. asperellum) was compared to the control management (with no PGPM). CL and C systems with co-inoculation showed the highest yields, while the CF and CLF systems, which include the arboreal component, showed lower yields because of tree shading. CL + co-inoculation treatment was the most significant with increases of 17.14% in shoot dry mass, 43% in 1000-grain weight, and 9.56% in yield (t ha-1) compared to the C + control treatment. Additionally, the CL + co-inoculation treatment overcame the CLF + control by 136.63% in yield. Results indicate that the PGPM co-inoculation in maize within the CL system is an effective strategy for optimizing crop production.

Semantic Segmentation Model-Based Boundary Line Recognition Method for Wheat Harvesting

The wheat harvesting boundary line is vital reference information for the path tracking of an autonomously driving combine harvester. However, unfavorable factors, such as a complex light environment, tree shade, weeds, and wheat stubble color interference in the field, make it challenging to identify the wheat harvest boundary line accurately and quickly. Therefore, this paper proposes a harvest boundary line recognition model for wheat harvesting based on the MV3_DeepLabV3+ network framework, which can quickly and accurately complete the identification in complex environments. The model uses the lightweight MobileNetV3_Large as the backbone network and the LeakyReLU activation function to avoid the neural death problem. Depth-separable convolution is introduced into Atrous Spatial Pyramid Pooling (ASPP) to reduce the complexity of network parameters. The cubic B-spline curve-fitting method extracts the wheat harvesting boundary line. A prototype harvester for wheat harvesting boundary recognition was built, and field tests were conducted. The test results show that the wheat harvest boundary line recognition model proposed in this paper achieves a segmentation accuracy of 98.04% for unharvested wheat regions in complex environments, with an IoU of 95.02%. When the combine harvester travels at 0~1.5 m/s, the normal speed for operation, the average processing time and pixel error for a single image are 0.15 s and 7.3 pixels, respectively. This method could achieve high recognition accuracy and fast recognition speed. This paper provides a practical reference for the autonomous harvesting operation of a combine harvester.

Adaptive shading: How microclimates and surface types amplify tree cooling effects?

Shading is the most critical process in tree cooling. Although the influence of tree features on shade supply has been extensively studied, the efficiency of shade performance in diverse environments remains poorly understood. The latter is crucial, as urban areas are highly heterogeneous, and local microclimates and land cover heterogeneity are among the most perceptible and observable aspects. We randomly selected several typical tree patches in Beijing to explore whether and how tree-shade efficiency varies with these factors. We used temporal variations as a proxy to study the impact of microclimatic heterogeneity on tree shade efficiency by comparing sunny and cloudy conditions, as well as different times of the day. In addition, we investigated the differences in shading efficiency over various surfaces by comparing pairs of regular urban surfaces (asphalt, concrete, permeable brick, and grass). We found that tree shade significantly improved the local thermal environment, but its effectiveness varied with the environmental conditions. Tree shading resulted in a higher cooling efficiency under greater external thermal stress. In addition, the cooling efficiency was greater on surfaces with higher temperatures. We also identified a notable characteristic of tree shading for cooling: the thermal environment beneath the tree shade tends to stabilize at a relatively constant value across various external conditions. These results provide practical guidelines for optimizing tree layouts in highly heterogeneous urban environments and enhancing limited tree resources for more effective and economical improvement of urban thermal conditions.

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes.

Valsa sordida and Botryosphaeria dothidea are two crucial necrotrophic fungal pathogens that damage many plant hosts, particularly species in the genus Populus. These two fungal pathogens occur mainly in poplar branches, stems, and twigs, causing classic symptoms such as canker lesions, canopy dieback, and wilting. Pathogen inoculation is the most efficient pathway to study the mechanism of plant disease. Besides the canker lesions around the inoculation sites on the stems, a novel developmental phenomenon, copious adventitious roots (ARs) with bright red color, were observed in poplar species after stem canker pathogen inoculations. In this study, we described the method for inducing ARs using fungal pathogens in poplar trees. The crucial step of this method is the pathogen inoculation after (phloem or epidermis) girdling manipulation. The second crucial step is the application of the moisturizing material. Compared to the moisturizing manipulation with Parafilm, wrapping the inoculated sites with household polyethylene (PE) plastic wrap can produce colorful, numerous, and robust ARs in 20 days after girdling-inoculation. Finally, white ARs sprouted from the inoculated rings in the poplar stems after shading treatment (wrapping the stems with aluminum foil). This method introduces a novel experimental system for studying root development and morphogenesis, which is crucial for understanding the biology of root development, morphogenesis, and response under disease stress. Furthermore, when combined with shading treatment, this study can provide a convenient experimental system for investigating light response-related processes, for example, the biosynthesis of flavonoids, anthocyanins, or other related metabolites, and genes or transcription factors involved in these processes.

MITIGATING THE URBAN HEAT ISLAND EFFECT: The Thermal Performance of Shade-Tree Planting in Downtown Los Angeles

The intensifying urban heat island (UHI) effect presents a growing challenge for urban environments, yet there is a lack of comprehensive strategies that account for how multiple factors influence tree-cooling effectiveness throughout the year. While most studies focus on the effects of individual factors, such as tree shading or transpiration, over specific time periods, fewer studies address the combined impact of various factors—such as seasonal variations, building shading, transpiration rates, tree placement, and spacing—on tree cooling across different seasons. This study fills this gap by investigating the thermal environment in downtown Los Angeles through ENVI-met simulations. A novel tree-planting strategy was developed to enhance cooling performance by adjusting tree positions based on these key factors. The results show that the new strategy reduces Universal Thermal Climate Index (UTCI) temperatures by 2.2 °C on the hottest day, 0.97 °C on the coldest day, and 1.52 °C annually. The study also evaluates the negative cooling effects in colder months, demonstrating that, in cities with climates similar to Los Angeles, the benefits of tree cooling in hot weather outweigh the drawbacks during winter. These findings provide a new method for optimizing tree placement in urban planning, contributing to more effective UHI mitigation strategies.

Shading Impairs Mycorrhizal Benefits on Plant Growth, Leaf Gas Exchange, and Active Ingredients in Polygonum cuspidatum

Polygonum cuspidatum, an important medicinal plant, often experiences shading from surrounding vegetation during its growth phase, raising questions about the impact of such conditions on the functionality of arbuscular mycorrhizal fungi. This study investigated the effects of an arbuscular mycorrhizal fungus (Funneliformis mosseae) on the growth, leaf gas exchange, and concentrations of active ingredient concentrations in leaves and roots of P. cuspidatum under shading (with a 72% shading rate) conditions. A nine-week shading intervention significantly suppressed root colonization by F. mosseae and the formation of soil mycorrhizal mycelium. Shading significantly inhibited the above-ground growth performance, biomass production, leaf photosynthetic rate, transpiration rate, stomatal conductance, and intercellular CO2 concentration, while F. mosseae significantly increased these variables in the absence of shading. Plant height, leaf biomass, stem biomass, leaf photosynthetic rate, transpiration rate, and stomatal conductance were all decreased by F. mosseae when the plants were shaded. The shading treatment also significantly diminished the concentrations of active components measured in both leaves and roots. Under no-shading conditions, F. mosseae significantly boosted the concentrations of polydatin, resveratrol, aloe-emodin, emodin, chrysophanol, and physcion in roots, as well as the concentrations of polydatin and chrysophanol in leaves. Conversely, in the presence of shading, F. mosseae distinctly reduced these active ingredient levels in roots, followed by an increase in leaf polydatin and chrysophanol concentrations. In summary, shading substantially impaired the mycorrhizal benefits on plant growth, leaf gas exchange, and root active ingredients in P. cuspidatum, highlighting the importance of sufficient light to maximize mycorrhizal contributions.

Variation of shade tree composition and carbon stock of smallholder coffee agroforestry systems along an elevation gradient in Khun Mae Kuang Forest area, northern Thailand

Variation of shade tree composition and carbon stock of smallholder coffee agroforestry systems along an elevation gradient in Khun Mae Kuang Forest area, northern Thailand

Social networks of pregnant gilts during outdoor feeding and the effects on their offspring

Social relationships are important aspects of the behavioural biology of pigs and can be affected by the type of housing pigs are kept in. Exploring agonistic interactions and affiliative behaviours can reveal effects on adult pigs and their descendants. This research investigated the social dynamics among gilts throughout pregnancy during collective feeding in an outdoor housing system and the effects of these dynamics on the stability of the group and the offspring of the group members. For this study, 15 gilts were oestrus synchronised and artificially inseminated with three different semen pools. The paddocks where the gilts were housed contained a mud pool, natural tree shade, and two nipple drinkers. Two daily meals (∼2.5 kg/day/gilt) were provided to the group on the floor. Feeding behaviour was recorded for three continuous days (20 min in the morning and again in the afternoon) every gestational (a total of 42 h of video recording). Saliva samples were collected at 6:00 a.m. and 6:00 p.m. on these same days. A trained observer evaluated all the videos using Boris software to determine agonistic and affiliative behaviours. After farrowing, the piglet data collected included sex, mortality, and body weight (BW) at 10, 25, 29, and 36 d of age. Elo scores were calculated to quantify the relative hierarchy among the gilts based on the behaviour assessment. Generalised linear mixed models (GLMMs) were used for data analyses, and the significance of fixed effects was determined at p < 0.05. The tendency of the gilts to feed together decreased as gestation progressed. Heavier gilts had lower salivary cortisol concentrations, and higher gilt rank was correlated with higher morning salivary cortisol concentrations and heavier piglets. Maternal modulation of offspring performance in pigs warrant further investigation. Assessment of gilts' social feeding behaviours is relevant for considering pigs' behaviour in genetic selection and improving commercial facilities and management practices to improve animal welfare.

Effect of local habitat and landscape attributes on bird communities in shade coffee plantations in the Colombian Andes

Agroforestry is increasingly promoted to support biodiversity conservation by increasing tree cover in agricultural landscapes, but the extent to which landscape context affects how benefits accrue remains uncertain. We used shade-coffee systems to ask how the proximity and extent of forest and forest-agriculture mosaic in the landscape influenced bird communities in 160 coffee plantations that differed in coffee plant density and shade tree richness and abundance in two departments of Colombia with differing regional forest cover. Our findings suggest that regional forest cover and landscape conditions can mediate the response of birds to local habitats on plantations. Avian richness and community completeness was positively related to the amount of forest-agriculture mosaic within landscapes surrounding coffee plantations only within the comparatively forested department of Antioquia (mean 32 % regional forest cover), particularly where plantations had high richness and abundance of trees. In the largely deforested department of Cauca (mean 1 %), neither distance to forest nor cover by forest-agriculture mosaics explained avian richness and community completeness, both of which were positively related only to local tree richness and abundance. We show that biodiversity benefits from increasing habitat quality at local and landscape scales, and habitat quality within plantations becomes increasingly influential as the amount of habitat in the broader landscape declines. Our results emphasize the role of landscape context in conservation planning to promote biodiversity in coffee-growing regions.

Effects of Different Shade Treatments on the Epidermal Wax Deposition of Hosta Genotypes with Different Glaucousness of Leaf Surface

Epidermal wax is strategically situated at the interface between plants and air; therefore, it plays a key role in plants’ interactions with their surroundings. It is also unstable and susceptible to light intensity. Hosta plants are shade-loving herbs with admirable flowers and leaves. Hosta ‘Halcyon’ and Hosta ensata F. Maek. are two species of Hosta with a glaucous and a glossy appearance, respectively. Light intensity can affect the composition of epicuticular wax on the leaf surface, which influences the leaf color phenotype and ornamental value. In this paper, the crystal micromorphology, content, and components of epicuticular wax on the leaves of two species of Hosta under different light conditions (10%-, 30%-, 50%-, 70%-, and 100%-intensity sunlight, relative light intensity (RLI)) have been studied using pot experiments. The results indicate that the epicuticular wax crystals of H. ‘Halcyon’ and H. ensata are tubular and platelet-like, respectively. The wax crystals of H. ‘Halcyon’ melted and formed a thick crust under 100% RLI, and those of H. ensata melted and formed a thick crust under 70% and 100% RLI conditions. The primary ingredients of the epicuticular wax of the two species of Hosta contained primary alcohols, alkanes, fatty acids, and esters; β-diketones were only detected in H. ‘Halcyon’. The quantity of epicuticular wax of H. ‘Halcyon’ reduced at first and then increased with an RLI increase, achieving its lowest value at 50% RLI, but that of H. ensata declined little by little. The amounts of C28 primary alcohols, C31 alkanes, and C18 fatty acids were significantly higher than those of other carbon atoms in the two genotypes of Hosta. The C31β-diketones content decreased with the increase in light intensity, which caused the white frost phenotype to gradually weaken in H. ‘Halcyon’.

Microclimate Effect on Cooling Energy for Buildings in Hot, Humid Climates: A Comparative Analysis of Shaded and Unshaded Environments

This paper explores the influence of microclimates on changes in air temperature and the often-overlooked aspect of their effect on energy savings across varying microclimatic conditions. The study compares the cooling energy requirements of two identical single-story buildings in distinct microclimates: one characterized by concrete ground devoid of shade and the other featuring soil ground with tree shade. Climatic environmental data were collected over 15 days in the concrete-exposed field and shaded area beneath the trees to conduct the investigation. These datasets were input into EnergyPlus 9.6 to model the energy demands and consumption of buildings subject to the specified climatic conditions. The validation of the simulated model against actual energy demand data from a classroom building demonstrated agreement. The findings reveal notable differences in air temperature, with the shaded area experiencing temperatures 0.8°C to 8.0°C lower than the concrete-exposed monitoring location. The building in the tree-shaded microclimate exhibited a lower peak cooling load than its concrete-exposed counterpart, resulting in a 35% reduction in the electrical energy requirements for the air-conditioning system. The study recommends implementing 0.08-m polyurethane insulation for the building walls and roof to equalize the energy demand and consumption of the concrete-exposed building with that of its shaded counterpart. Furthermore, building design in shaded areas can maximize the window glass area while consuming less energy than buildings on concrete-exposed grounds. The study advocates leveraging the microclimate associated with surrounding buildings in the design process to enhance the overall energy savings.

PHYTOCHROME-INTERACTING FACTOR 7 and RELATIVE OF EARLY FLOWERING 6 act in shade avoidance memory in Arabidopsis

Shade avoidance helps plants maximize their access to light for growth under crowding. It is unknown, however, whether a priming shade avoidance mechanism exists that allows plants to respond more effectively to successive shade conditions. Here, we show that the shade-intolerant plant Arabidopsis can remember a first experienced shade event and respond more efficiently to the next event on hypocotyl elongation. The transcriptional regulator PHYTOCHROME-INTERACTING FACTOR 7 (PIF7) and the histone H3K27-demethylase RELATIVE OF EARLY FLOWERING 6 (REF6) are identified as being required for this shade avoidance memory. RNA-sequencing analysis reveals that shade induction of shade-memory-related genes is impaired in the pif7 and ref6 mutants. Based on the analyses of enrichments of H3K27me3, REF6 and PIF7, we find that priming shade treatment induces PIF7 accumulation, which further recruits REF6 to demethylate H3K27me3 on the chromatin of certain shade-memory-related genes, leading to a state poised for their transcription. Upon a second shade treatment, enhanced shade-mediated inductions of these genes result in stronger hypocotyl growth responses. We conclude that the transcriptional memory mediated by epigenetic modification plays a key role in the ability of primed plants to remember previously experienced shade and acquire enhanced responses to recurring shade conditions.

Metabolomic and transcriptomic analyses reveal the mechanism of polysaccharide and secondary metabolite biosynthesis in Bletilla striata tubers in response to shading

Polysaccharides and various secondary metabolites are the major bioactive ingredients in Bletilla striata tubers and their biosynthesis and accumulation are influenced by light intensity. However, the mechanisms underlying shading effects remain largely unknown. In the present study, we used a combined analysis of the physiology, metabolome, and transcriptome to investigate the physiological activities and bioactive component accumulation of B. striata under different shading treatments (S0, S50, S70, and S90). The dry weight of shoots and tubers, net photosynthetic rate, and polysaccharide content were highest in S50 and lowest in S90. The content of precursors (sucrose, Glucose-6P, and Mannose-6P) for polysaccharide synthesis significantly increased in S50. However, the expression levels of genes involved in starch biosynthesis decreased in S50. Several structural genes involved in secondary metabolism, including cinnamic acid 4-hydroxylase (C4H), chalcone synthase (CHS), and 1-Deoxy-D-xylulose-5-phosphate synthase (DXS), showed decreased expression in S50. However, the shading effect on the biosynthesis of secondary metabolites (phenylpropanoids, flavonoids, and terpenoids) was inconsistent. Our study provides the molecular mechanisms underlying the effects of shading on the biosynthesis of polysaccharides and secondary metabolites in B. striata and offers a theoretical basis for the artificial cultivation and industrial production of bioactive ingredients.

Perennial species diversity, ecosystem carbon stocks and carbon income in coffee-based agroforestry systems along an elevation gradient in South-eastern Ethiopia

In the current context of deforestation, coffee-based agroforestry system (CAFS) is credited for climate change (CC) mitigation and biodiversity conservation while supporting local livelihoods. Despite integrating shade tree species in CAFS, empirical studies to support this assertion are inadequate in Eastern Africa, and hence, its ecosystem services provisions are less understood. We evaluated perennial species diversity, carbon (C) stocks in the biomass and soil organic C (SOC) along an elevation gradient of 72 plots of shade coffee, while 36 plots were selected for without-shade coffee systems within three elevations, namely, low (1600–1750 masl), mid (1750–1850 masl) and high (1850–2000 masl) elevations in Southeastern Ethiopia. The perennial species diversity and biomass, SOC, fine root and litter C stocks were evaluated. Perennial species Shannon diversity significantly differed among the studied elevations (p < 0.001). Shaded coffee had significantly higher ecosystem C stocks than without shaded coffee systems (p < 0.05). The highest C stocks were found in the soil in both coffee systems. However, we found a weak relationship between the Shannon diversity and biomass C. The C income in shaded coffee was 70 % higher than without shaded coffee systems. The present study showed that shaded coffee accumulates more C and provides additional benefits from C credits. Hence, CAFS deliver ecosystem services that enhance biodiversity conservation and CC mitigation while generating an additional C income for farmers. However, we learned that the impact of perennial plant diversity on C stock and C income is context and site-specific.

GIS-based management of a campus tree inventory for assessing optimal shade tree locations: a case study of National Cheng Kung University, Taiwan

GIS-based management of a campus tree inventory for assessing optimal shade tree locations: a case study of National Cheng Kung University, Taiwan

Light condition during grain-filling stage of main crop strongly influences ratooning ability of low-stubble ratoon rice

Compared with high-stubble ratoon rice (RR), low-stubble RR is superior in yield potential, grain quality, and economic benefit. However, the unstable ratooning ability limits the grain yield of low-stubble RR production. Light condition during the grain-filling stage of main crop (GFMC) may be important for rice ratooning. To elucidate the role of light condition during GFMC in affecting ratooning ability, the key response periods, and their underlying mechanisms, field experiments were conducted using two indica cultivars in 2021 and 2022. To create varied light conditions at the canopy base during GFMC, two planting density treatments combining three nitrogen (N) treatments were established in 2021, and three density treatments combining two N treatments and four shading treatments were established in 2022 for the main crop. Light intensity (LI), light quality as reflected by the ratio of red light/far red light (R/FR), and light transmission ratio (LTR) at the canopy base during GFMC and ratooning ability were dramatically altered by N fertilization but not by planting density. With increased N application, LTR, root bleeding rate, and maximum ratooning rate significantly decreased in 2021. In 2022, low N rate increased LI, R/FR, and maximum ratooning rate by 155.7-241.4%, 47.4-65.3%, and 15.6-27.5%, respectively, but reduced missing hill percentage (proportion of hills without regenerated tillers to the total number of hills) by 30.0-62.1% compared with high N rate. The missing hill percentage was negatively correlated with the indices of light condition, while the maximum ratooning rate was positively correlated with them for both cultivars. Root activity and the ratios of abscisic acid (ABA) to cytokinins (CTK), indole-3-acetic acid (IAA), and IAA+CTK could explain the effect of light condition during GFMC on ratooning ability. Shading experiment confirmed the effect of light condition on ratooning ability and further revealed that only shading during middle and late GFMC affected ratooning ability. These findings provide new insights into the regulation of ratooning ability, which are useful for developing management practices to increase the grain yield and yield stability of low-stubble RR.

Physiological Characters of Vanilla Plant (Vanilla planifolia Andrew) in Various Types of Shade Trees

Vanilla plants are CAM plants and are very sensitive to direct sunlight, so vanilla plants need shade plants to reduce the intensity of sunlight. Research on the physiological characteristics of vanilla plants on various types of shade trees was carried out to finding out the influence of types of shade trees on the physiological characteristics of vanilla plants. The research was conducted using a randomized completely block design with four groups. Planting was carried out using four types of shade plants, namely Gliricidia sepium, Syzygium aromaticums, Erythrina variegata and Leucaena leucocephala plants. To determine microclimate conditions, temperature, air humidity and sunlight intensity were observed three times a day (morning, afternoon and evening). Physiological observation parameters include content of chlorophyll A, chlorophyll B levels, total chlorophyll, proline, relative water content. The results of the research showed that temperature, air humidity and the intensity of sunlight received are different for each type of shade plant. Types of shade plants significantly influence content of chlorophyll A, chlorophyll B, total chlorophyll, proline and relative water content. The conclusion was the type of shade plant affects the microclimatic conditions of the vanilla plant, thereby influencing the physiological properties of the vanilla plant.

Environmental Awareness and Walking Behavior to the Grocery Store

The relationship between environmental awareness and actionable change is complex. While extensive literature examines the links between green ideology and eco-friendly behavior, few studies focus on how green ideology influences transportation choices and the built environment. This study investigates the relationship between environmental awareness and walking behavior, addressing three research questions aimed at: identifying socio-demographic characteristics linked to a high level of environmental awareness; exploring how environmental awareness affects perceptions of walkability; and determining whether individuals committed to community environmentalism are more likely to walk to the grocery store. The findings reveal that higher income is associated with considerations of local air quality, noise, and the importance of positive environmental transportation choices. Car ownership negatively impacts the willingness to adjust routes or travel time to minimize environmental impact. Environmentally aware individuals perceive the grocery store as closer, feel safer walking, view sidewalks as more available and in a better condition, and notice more tree shading. Those who consider air quality and noise levels in their walking decisions are more likely to notice tree shading. Individuals committed to community environmentalism are significantly more likely to walk to the grocery store, underscoring the crucial role of environmental values in shaping transportation behaviors and walkability perceptions.