The demand for natural dyes in the modern world is increasing day by day. These resources have become important for their use in food, pharmaceuticals and textiles Industries, instead of their synthetic dyes. Using these resources we can protect human health and prolong life on the earth. This research focuses on Turbinaria conoides, a brown seaweed collected from Olakuda, Ramanathapuram coast, Tamil Nadu, India (9.2876° N, 79.3129° E). A crude dye was extracted from the seaweed and used to treat cotton fabric using the pre-mordanting technique. To achieve different shades of colors, Turbinaria conoides was combined with other dye-yielding plant materials, including Vachellia nilotica, Oldenlandia umbellate, Rubia cordifolia, and Alkanna tinctoria. The phytochemical analysis confirmed the presence of bioactive compounds in Turbinaria conoides. Colorfastness tests were conducted to evaluate the dye's stability on the fabric. The study concludes that natural dyes hold great potential and interest, especially Turbinaria conoides. India's vast plant resource base presents opportunities for further research and documentation of natural dye usage. Sustainable and eco-friendly practices in the textile industry can be fostered through algal-sourced pigments, contributing to environmental protection and an eco-aware society. Future aspects include a focus on environment-friendly dyes, affordable production methods, and exploring the full potential of natural dyestuffs for various applications, including waste utilization and job creation.