Bioproduction and Application of Orange and Yellow Pigments Obtained from Chryseomicrobium palamuruense and Micrococcus luteus

Abstract

Colour is a fundamental and most likely one of the primary attributes to be observed by the human senses, making it an essential part of human life and culture. This study investigates the bioproduction and potential application of bacterial pigments obtained from abattoir wastewater. Bacteria isolates recovered from abattoir wastewater were screened for pigment production and identified using the 16S rRNA gene sequence. Optimal physicochemical conditions of parameters were investigated and harnessed for bulk pigment production. Pigments produced were characterised using UV-Visible Spectrophotometer and Fourier Transform Infra-Red (FTIR) Analysis. The antibacterial and dyeing ability of the extracted pigments were evaluated. Two bacteria namely, Chryseomicrobium palamuruense strain PUI and Micrococcus luteus strain 1k produced orange and yellow pigments respectively. The UV-visible spectrum of the orange pigment produced by Chryseomicrobium palamuruense showed surface plasmon resonance peaks at 262, 426, and 453 nm while that of the yellow pigment produced by Micrococcus luteus showed a single peak at 260 nm. The FTIR analysis of the extracted pigments indicated the presence of some functional groups namely hydroxyl (OH), C-H, primary and secondary C-O, and skeletal C-C vibrations. Chryseomicrobium palamuruense and Micrococcus luteus showed no antibacterial activity against the selected typed bacterial isolates assessed in this study. The orange pigment extracted from Chryseomicrobium palamuruense was retained on cotton and polyester producing different shades of colour on the two fabrics when used as a dyeing agent. The pigment-producing potentials of these organisms suggest their relevance as promising dyes in textile industry.