Severe Health Research Articles

Thresholds for the value judgement of health technologies in the United Arab Emirates: a consensus approach through voting sessions

BackgroundIn the evolving healthcare landscape of the United Arab Emirates (UAE), establishing cost-effectiveness thresholds (CETs) is pivotal to informing decision-makers about the value of health technologies.ObjectiveThis study aimed to establish...

Environmental And Health Implications of Waste Disposal Sites In The Lesotho Lowlands

Many Southern African countries, including Lesotho, face severe health and environmental issues attributed to waste disposal. The purpose of this study is to assessed the environmental and health impacts on residents living in proximity to disposal sites in the lowlands of Lesotho. A descriptive cross-sectional design was employed, using a semi-structured questionnaire and observation method. A mapping device was used, and photographs were taken to validate the findings. Descriptive statistics and the Chi-square test (?²) were employed to analyze the data. Results indicate that in Lesotho, some landfill sites are located as close as 50 meters from residential areas, and most (60%) received hazardous mixed waste streams. Waste burning and backyard dumping were the most frequently used disposal methods by residents. Several perceived health outcomes and environmental issues were noted, with respiratory and gastrointestinal health issues being the most prevalent. There was a statistically significant association between waste burning and respiratory diseases (P < 0.010) and between backyard dumping and gastrointestinal diseases (P < 0.003). The study concludes that regulatory instruments need to be revised and enforced, requiring hazardous waste producers to take responsibility for end-of-life waste and banning the entry of such waste into disposal sites. The application of waste management strategies to initiate other preferable end-of-life waste procedures, such as recycling of WEEE and waste-to-energy technologies for organic waste in Lesotho, could improve the quality of health for residents and their environment.

Modeling the health impact of wastewater contamination events in drinking water networks

Pathogen intrusion in drinking water systems can pose severe health risks. To better prepare in planning and responding to such events, computational models that capture the intrusion and health impact dynamics are needed. This study presents a novel benchmark testbed that integrates current knowledge on pathogen transport and fate in chlorinated systems and can assess infection risk from contamination events. The model considers organic matter degradation, chlorine decay mechanisms, pathogen inactivation kinetics, as well as stochastic water demands.We studied modeling of wastewater intrusion events that can occur anywhere within a chlorinated and non-chlorinated network. We applied the Quantitative Microbial Risk Assessment framework focusing on three pathogens: enterovirus, Campylobacter, and Cryptosporidium, and their respective dose-response models. Synthetic household-level water demand time series were used to model the individual water consumption timing and calculate the infection risk (exposure via ingestion).Model outcomes indicate that while chlorination aids mitigation, larger contaminations can still lead to infections due to chlorine resistance (for Cryptosporidium) and chlorine depletion at the contamination point. In our example scenarios, chlorine-susceptible pathogens infected of the downstream population, while chlorine-resistant ones infected the entire downstream population. Enterovirus infection risk is higher, despite the concentrations in the contamination source being lower, due to the lower susceptibility to chlorine than Campylobacter. In non-chlorinated networks, the modeled wastewater contamination events led to infection risk in the total population, depending on the contamination location. Hydraulic uncertainty had a limited influence on infection risk. Furthermore, Campylobacter’s infection risk is more sensitive to the initial concentration in the contamination source whereas enterovirus infection risk to the inactivation rate. The model further indicates that the time window for effective mitigation of the magnitude of a waterborne outbreak is short (within hours).

The hidden crisis: Health impacts of tobacco and nicotine products on Indian women

ABSTRACT Tobacco use among women in India is becoming a critical public health issue. Once viewed predominantly as a male habit, tobacco consumption among Indian women has seen a rising trend. This shift is driven by factors such as evolving societal norms, aggressive marketing by the tobacco industry, and increasing urbanization. Women are now using a variety of tobacco products, including smokeless tobacco and electronic nicotine delivery systems (ENDS). The health effects are particularly alarming, with elevated risks for cancers, cardiovascular diseases, respiratory conditions, and reproductive health problems. Despite these concerns, public health initiatives and policies often fail to address the specific needs of women. editorial examines the social and cultural drivers of tobacco use among Indian women, outlines the severe health risks, and calls for immediate action through gender-sensitive tobacco control policies. It provides recommendations, including robust regulation, community involvement, and specialized cessation programs to reduce the growing tobacco use among women.

Commentary on Antithrombotic Effect of Protopanaxatriol Saponins from Panax notoginseng Using Zebrafish Model.

Thrombosis continues to pose a significant challenge in cardiovascular and cerebrovascular diseases, contributing to severe health complications such as myocardial infarction, acute ischemic stroke, and venous thromboembolism. Despite the wide array of anti-thrombotic drugs available, these treatments frequently carry substantial risks, notably including bleeding complications. In this paper, we comment the findings reported by Liu et al. about the anti-thrombotic potential of protopanaxatriol saponins from panax notoginseng.

Insight into the Natural Biomolecules (BMs): Promising Candidates as Zika Virus Inhibitors.

Zika virus (ZIKV) is among the relatively new infectious disease threats that include SARS-CoV-2, coronavirus, monkeypox (Mpox) virus, etc. ZIKV has been reported to cause severe health risks to the fetus. To date, satisfactory treatment is still not available for the treatment of ZIKV infection. This review examines the last five years of work using natural biomolecules (BMs) to counteract the ZIKV through virtual screening and in vitro investigations. Virtual screening has identified doramectin, pinocembrin, hesperidins, epigallocatechin gallate, pedalitin, and quercetin as potentially active versus ZIKV infection. In vitro, testing has shown that nordihydroguaiaretic acid, mefloquine, isoquercitrin, glycyrrhetinic acid, patentiflorin-A, rottlerin, and harringtonine can reduce ZIKV infections in cell lines. However, in vivo, testing is limited, fortunately, emetine, rottlerin, patentiflorin-A, and lycorine have shown in vivo anti- ZIKV potential. This review focuses on natural biomolecules that show a particularly high selective index (>10). There is limited in vivo and clinical trial data for natural BMs, which needs to be an active area of investigation. This review aims to compile the known reference data and discuss the barriers associated with discovering and using natural BM agents to control ZIKV infection.

New Schiff base covalently bonded Graphene Oxide for removing Chromium(VI) from Surface Runoff.

Hexavalent chromium (Cr(VI)) poses severe health and environmental risk, especially in industrial vicinities where runoff concentrations are elevated by evaporation and sedimentation dynamics. This study investigates the utilization of modified graphene oxide (GO) with abundant active groups as an adsorbent. The novel adsorbent, GO/ATA, was synthesized by covalently attaching a Schiff base (ATA) to GO via a coupling reaction, enhancing its adsorptive properties. The physicochemical properties of GO/ATA were meticulously characterized to ascertain structural and morphological attributes. GO/ATA exhibit exceptional adsorptive capabilities, surpassing the performance parameters of other adsorbents with a maximum of 243.3 mg g-1 at 298 K. The electrostatic attraction between the N-containing functional groups in the graphene oxide and Schiff base composite (GO/ATA) and Cr(VI) is the main mechanism behind the adsorption process; Subsequently, a reduction reaction occurs, facilitated by the thiol and N-containing functional groups in GO/ATA composite, resulting in the transformation of Cr(VI) into Cr(III). This transformation is essential for the following chelation process occurring on the surface of the adsorbent material. Evaluations of recyclability indicated that GO/ATA retains substantial adsorption efficacy, with a reduction from 91.2% to 72.7% over five cycles, thus affirming its recyclability and practical application in the remediation of Cr(VI)-contaminated waterborne environment. Additionally, GO/ATA's effectiveness in removing Cr(VI) from surface runoff was specifically tested, emphasizing its economic and environmental viability.

Investigating the Toxicity of Malachite Green and Copper Sulfate in Brine Shrimp: in-vivo and Computational Study

Colour is crucial for enhancing the appetizing value and consumer acceptance of food products. The commonly used food colourants and food preservatives such as Malachite Green (MG) and Copper Sulfate (CS) can cause severe health problems. This study investigates the toxicity of these food-grade colourants through acute exposure using in vivo cytotoxicity using the brine shrimp model including 3D surface analysis (3DSA) and in-silico studies Brine shrimp were treated with various concentrations of MG and CS. The cytotoxic effect was confirmed by brine shrimp lethality assay and 3DSA. Molecular docking and Molecular Dynamic simulation were done using hAChE binding cavity. Results showed that concentrations (2.5-10µg/ml) of MG and CS significantly decreased locomotor behaviour within 1hour, while higher concentrations (10-100µg/ml) caused high mortality rates. Morphological studies revealed that there is a significant reduction (p<0.05) in shrimp length treated with MG and CS. The 3DSA indicates that there is an inappropriate surface of the shrimp morphology. Interestingly, MG-treated shrimps had shown significant inhibition of AChE in homogenates, indicating cholinergic nerve-mediated toxicity. Computational studies showed MG confined active binding with human acetylcholinesterase (hAChE), with a binding energy MMGBSA of -51.3kcal/mol. MD simulation confirmed reversible binding stability inside the hAChE pocket. It can be concluded that acute exposure to brine shrimps with MG and CS exhibited cytotoxicity as evidenced by the increase in mortality of the shrimps. This study further warrants the investigation of MG and CS residues from commonly used fruits and vegetables and their putative toxic effect using in-vivo studies.

Does good knowledge affect the disapproving attitudes of female adolescents regarding early marriage? A cross-sectional study

Early marriage is a severe public health problem issue. This study aimed to analyze the knowledge and attitudes of female adolescents regarding early marriage. A cross-sectional design was used and included 245 female adolescents. Data was collected using a structured questionnaire. Multivariable logistic regression was used to analyze the relationship between knowledge and attitudes regarding early marriage. Most of the respondents disagree with early marriage. Female adolescents with good knowledge were 0.51 times more likely to disagree with early marriage than those with less knowledge. Female adolescents with higher mothers’ education were 0.66 times more likely to disagree with early marriage than female adolescents with lower mothers’ education. Good female adolescents' knowledge and mother's education support female adolescents' disapproval of early marriage. We conclude that the better the understanding of female adolescents about early marriage, the less they agree with early marriage.

A Holistic Harm Reduction Center Model: A Commentary

Substance addiction is a chronic, relapsing disorder characterized by compulsive drug seeking, loss of control over consumption, and a negative emotional state when access to the drug is restricted. Substance Use Disorders (SUDs) are associated with severe health issues including suicide and co-occurring diseases like HIV and hepatitis C, and mental health problems. Although the present SUD treatment models prioritize abstinence, achieving abstinence during a treatment episode is challenging as majority of individuals with SUDs continue using drugs during treatment. To address this challenge in the current SUD treatment model, harm reduction has emerged as a strategy that focuses on interventions such as naloxone distribution, syringe services, safe drug use education and other strategies that reduce harmful outcomes such as substance-related overdose death and disease transmission while improving quality of life. This commentary proposes a Holistic Harm Reduction Model that can be incorporated by harm reduction centers aimed at reducing risky drug use behavior and overdose deaths, and ultimately improving public health outcomes in the community. The proposed holistic model includes new components such as trauma-informed care and referral and a brief mindfulness intervention along with the already existing harm reduction approaches such as Licensed Practical Nurses (LPNs) and harm reduction managers to provide comprehensive healthcare services, distribution of supplies; education; referral and linkage to other services, including medications for substance use disorder; and participant data collection and analysis.

Treatment patterns and characteristics of patients with Post-Traumatic Stress Disorder (PTSD): A retrospective claims analysis among commercially insured population.

This retrospective claims analysis explored the treatment utilization and characteristics among patients with post-traumatic stress disorder (PTSD) of different severity. The index date was the first PTSD claim. The analysis observed 12 months pre- and 24 months post-index. Adults with insurance gaps, cancer, or acute PTSD during the observation were excluded. Patients were categorized into three severity cohorts based on treatment and healthcare services utilization for PTSD: 1. Baseline PTSD (BP) (no PTSD visits post-index, no FDA-approved medications/ psychotherapy, and no severe mental health comorbidities); 2. PTSD without Comorbidities (PwoC) (≥1 PTSD visits post-index and no severe mental health conditions); 3. PTSD with Comorbidities (PwC) (≥1 PTSD visits post-index and severe mental health comorbidities present). For the primary analysis, cohorts were propensity-score matched. A sub-analysis examined patients with PTSD and Substance or Alcohol Use Disorder (SUD/AUD). The primary analysis observed 1714 BP, 1681 PwoC, and 1681 PwC patients. Treatment utilization rates were highest among PwC vs. other cohorts (84.5% psychotherapy, 76.1% off-label medications, and 26.1% FDA-approved medications [p<0.001]). PwC cohort also had the highest number of psychotherapy sessions and medication prescriptions per patient (20.1 sessions, 12.6 off-label prescriptions, and 2.0 FDA-approved prescriptions [p<0.001]). The proportion of days covered (PDC) indicated low medication adherence (0.25-0.40) with adherent patient rates (PDC ≥0.80) between 8.0-17.5%. The SUD/AUD sub-analysis identified 85 BP, 537 PwoC, and 3154 PwC patients. Conclusions were similar, with PwC cohort having highest treatment utilization rates (87.1% psychotherapy, 85.0% off-label medications, 28.2% FDA-approved medications [p≤0.013] with 24.4 sessions, 16.1 off-label prescriptions, and 2.0 FDA-approved prescriptions per patient [p≤0.002]). Only 4.7-11.4% of patients were adherent. PwC patients received psychotherapy and pharmacotherapy more frequently than PwoC and BP patients. Medication adherence among treated patients was low. Patients with SUD/AUD had numerically higher treatment utilization and lower medication adherence.

The impact of alcohol misuse in trauma patients: A scoping review protocol.

Alcohol use is a contributing factor in many cases of traumatic injury. There is conflicting evidence on the impact of alcohol use at the time of physical trauma on severity of injury and hospital course. Similarly, the significance of alcohol use disorder on outcomes in hospitalized trauma patients is unclear. This scoping review aims to provide a concise overview of the current literature surrounding peri-trauma alcohol use and alcohol use disorder on injury severity, in-hospital complications, patient outcomes, and long-term health impact of alcohol use in trauma. We will also explore the associated healthcare costs of this patient population. A systematic search of the following databases MEDLINE, EMBASE, and Cochrane Library will be completed to extract all studies that meet our inclusion criteria from January 2000 onwards. Case reports will be excluded. Two reviewers will screen all citations, abstracts, and full text articles. A third reviewer will act as tiebreaker at each stage of the screening process. A narrative synthesis without meta-analysis will be conducted and assessed based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. This review will contribute to the literature by providing a concise overview of the current data on the impact of alcohol on outcomes following trauma. We will explore the overall themes in the literature, limitations, and future directions to focus forthcoming research in this patient population. This project is registered via the Open Science Framework. The public registration is uniquely identified with the following DOI: https://doi.org/10.17605/OSF.IO/Z84WK. There were no funders or sponsors involved in the development of this protocol.

Genetic Variability and Correlation Studies in Red Rice (Oryza sativa L.) for Yield Related Traits and Grain Zinc, Iron and Protein Content

Aims: Nutrient deficiencies, particularly zinc (Zn), iron (Fe) and protein, pose severe health risks especially in children and lactating women. Red rice is significantly rich in iron, zinc and protein compared to white rice. The present investigation was undertaken in this context to elucidate information on variability and character association in red rice genotypes to identify effective selection criteria for grain yield and quality improvement of red rice genotypes. Study Design: Randomized complete block design with two replications. Place and Duration of Study: Zonal Agricultural and Horticultural Research Station, Brahmavar, Udupi during Kharif 2023. Methodology: A total of 108 red rice genotypes were used in the study and observations were recorded for ten characters. Protein was estimated by Kjeldhal method, zinc and iron content by Atomic absorption spectrophotometer (AAS) method. The data collected was subjected to statistical procedures using R software. Results: Analysis of variance revealed significant variation among the genotypes for all the characters studied. Grain Zn (11.4-44.7ppm), Fe (13.11-37.12ppm) and protein (5.0-11.43%) showed wide range of variability. High genotypic and phenotypic coefficient of variation was observed for Zn, number of productive tillers per plant, Fe, grain yield per plant, number of grains per panicle and plant height. Correlation studies revealed that grain yield per plant exhibited significant positive association with yield component traits. Protein and Fe content had negative association with grain yield. Conclusion: The traits that have high PCV, GCV, heritability and genetic advance as per cent mean are controlled by additive gene action which is very valuable for selection. The traits that are positively correlated with grain yield can be used for indirect selection which aids crop improvement. Karthika, Bilihallga and Shahara genotypes have high protein, iron and zinc respectively, which can be used in the further breeding program to develop nutritionally rich varieties.

Smart noise pollution monitoring system

Increasing pollution poses serious health risks, necessitating urgent control measures for a healthier future for all. Among various pollution types, sound pollution significantly impacts quality of life and well-being, especially in densely populated urban areas. This proposal describes the creation and deployment of an Internet of Things (IoT)-based noise pollution monitoring system intended to track sound levels in certain regions in real time. The core of this system comprises advanced sensors strategically placed to measure sound intensity accurately. These sensors can detect even minor variations in noise levels, providing precise and reliable data. After being gathered, the data is sent to a microcontroller, which effectively processes it. This microcontroller ensures the data is accurately captured and sent over the internet to an online server. Once the data reaches the online server, it becomes accessible for analysis and monitoring by authorities. The system's real-time data capability is crucial for timely intervention. Authorities can continuously monitor noise levels and identify trends or spikes in sound pollution, allowing them to address issues promptly. This feature is particularly beneficial for sensitive areas such as schools, hospitals, and residential zones, areas with heavy traffic, library, Where excessive noise can have severe health effects, including stress, sleep disturbances, and hearing loss. The system includes an alert mechanism that notifies relevant authorities when noise levels exceed predefined thresholds. These alerts enable quick responses to mitigate noise pollution. For instance, in a school, reducing noise levels can enhance the learning atmosphere and improve students' concentration. In hospitals, maintaining a quiet environment is crucial for patient recovery. The integration of IoT technology in this noise pollution monitoring system not only enhances efficiency but also provides a scalable solution adaptable to various urban and rural settings. This scalability ensures the system can be expanded to cover larger areas or multiple locations as needed.

Polydopamine-Mediated Antimicrobial Lipopeptide Surface Coating for Medical Devices.

Biofilm formation on medical implants such as catheters is a major issue which needs to be addressed as it leads to severe health care associated infections. This study explored the design and synthesis of a polydopamine-lipopeptide based antimicrobial coating. The coating was used to modify the surface of Ultrathane Catheters. The lipopeptide SL1.15 with an N-terminal cysteine was covalently conjugated to the polydopamine modified catheters via a Michael addition reaction between the thiol moiety in the peptide and the aromatic ring in the polydopamine layer. The immobilization of the peptide on the polydopamine coated catheters was confirmed using water contact angle, X-ray photoelectron spectroscopy, atomic force microscopy, and scanning electron microscopy (SEM). The antimicrobial activity of the coated catheters investigated using drug resistant and clinical strains of Gram-positive (MRSA and S. aureus) and Gram-negative (E. coli, A. baumannii, and P. aeruginosa) bacteria revealed that lipopeptide immobilization inhibited >90% bacterial adhesion to the catheter surface. Additionally, biofilm assays against MRSA and E. coli revealed that the lipopeptide immobilized catheters inhibited >85% bacterial growth after 1 week incubation. Finally, the cytotoxicity profile of the catheters using the human dermal fibroblast, and the human embryonic kidney cell lines demonstrated that the polydopamine-lipopeptide coating was not toxic after 72 h incubation.

Low-cost portable sensor for rapid and sensitive detection of Pb2+ ions using capacitance sensing integrated with microfluidic enrichment.

Lead ion (Pb2+) pollution is a critical global issue due to its ability to accumulate in the human body, resulting in severe health problems. Despite extensive research efforts devoted to the detection of Pb2+ contamination, practical, rapid, and field-deployable sensors for Pb2+ is yet to be developed to effectively safeguard the environment and public health. Herein, a label-free affinity-based sensing device is developed based on printed circuit board (PCB) for low-cost, easy-to-use, and real-time on-site detection of Pb2+ ions. The sensors are prepared by forming a self-assembled monolayer of glutathione (GSH) on the surface of gold-plated PCB electrodes, which serves as a molecular probe to recognize Pb2+. Rapid and sensitive detection is achieved by using capacitance sensing integrated with microfluidic enrichment. The sensor's interfacial capacitance is used to indicate specific binding, while the capacitance reading process simultaneously induces alternating current electrothermal (ACET) acceleration of analyte's travel towards the probes. Thus, the enrichment and detection are integrated into a single step, making pre-concentration unnecessary and shortening the assay time to 30s. This Pb2+ sensor has demonstrated one of the lowest limits of detection reported so far (1.85 fM) with a linear range of 0.01-10pM. To evaluate the sensor's specificity, non-target metal ions are tested, all showing negligible responses. Testing of tap water sample also yields reasonable results, validating the sensor's robustness. The above-mentioned features, together with a commercial portable readout, make this sensor well-suited for point-of-use Pb2+ detection at low cost.

A longitudinal observational study on activated partial thromboplastin time in hypothyroidism and effect of thyroxine supplementation

Background: Hypothyroidism is a common condition characterized by thyroid hormone deficiency, which, if untreated, can lead to severe health consequences and even death. Due to the wide range of clinical presentations and non-specific symptoms, hypothyroidism is primarily diagnosed biochemically. It can result from congenital thyroid abnormalities or iodine deficiency. Aims and objectives: Aim: To evaluate the correlation between hypothyroidism and Activated Partial Thromboplastin Time (aPTT) and assess the effect of thyroxine supplementation. Objective: To study the impact of levothyroxine supplementation on aPTT in hypothyroid patients. Materials and methods: This observational study was conducted at the General Medicine outpatient clinic and inpatient wards at Al-Ameen Medical College and Hospital, Vijayapura, from September 2022 to March 2024. A total of 80 hypothyroid patients of both sexes were included. TSH, Free T4, and aPTT levels were measured with appropriate aseptic precautions. Results: The majority of subjects were females (90%), with the highest proportion (31.3%) aged 26-35 years. The mean ± SD of pre-thyroxine TSH was 25.453 ± 19.9073, which significantly decreased to 4.299 ± 0.6738 post-thyroxine. aPTT values also decreased significantly post-treatment. A positive correlation was found between TSH and aPTT, while aPTT had a negative correlation with T3 and T4 levels. Conclusion: aPTT is significantly elevated in hypothyroid patients and shows a positive correlation with TSH levels. Regular monitoring of aPTT should be included in the follow-up of hypothyroid patients.

A Novel Labeling Method of Physiological-based Pressure Pain Assessment Among Patients With and Without Chronic Low Back Pain

Pain, especially chronic pain, is a complicated and subjective experience, threatening global healthcare as one of the most severe health problems. Traditionally, pain is assessed by Visual Analog Scale to indicate the pain intensity by the patient’s self-report, causing them to become biased by various psychosocial factors. In this study, we performed two distinct labeling methods to assess the pressure pain in Quantitative Sensory Testing and to differentiate healthy controls and chronic low back pain patients: time period labels and percentage timestamp labels. Physiological signals such as blood volume pulse and galvanic skin response were collected. The time period labeling method was to segment via fixed time windows. The percentage timestamp labeling method was to select the timestamp labels based on the percentage of the threshold or the tolerance time. Both methods demonstrate different advantages when visualizing the information of different pain states and different participant groups.

Water Quality Analysis and Risk Assessment of Potential Toxic Elements in Selected Surface Water Around Mining Sites in Abuakwa South Municipal, Ghana

ABSTRACTSurface water quality at Abuakwa South Municipal has received threats from surrounding mining communities, which are becoming a major concern to the public. As a result, this study aimed to assess water quality by examining the concentration of lead (Pb), cadmium (Cd), and zinc (Zn) pollution in surface water. Fifteen samples were obtained from surface water using a 1.5 L plastic bottle. The mean concentrations of Pb and Cd were above WHO guidelines of 0.01 and 0.003 mg/l, respectively, except for Zn which was below the stipulated limit of 3.0 mg/l. The geochemical process model shows that Cd, Pb, and Zn are adsorbing (released from the drainage to the surrounding environment) in sites 1 and 3 whereas Pb is desorbing (added to the drainage) in site 2. A hazard quotient greater than 1 was recorded for Cd in adults through the ingestion route of exposure at all the sites. Cancer risk also shows that the inhabitants are at risk through the ingestion route of exposure. The findings of this study suggest intake of water draining within the vicinity is unsafe and poses severe health risks.

A novel Non-rodent animal model of hydrochloric acid-induced acute and chronic lung injury

Hydrochloric acid is one of the most prevalent and hazardous chemicals. Accidental spills occur in industrial plants or during transportation. Exposure to HCl can induce severe health impairment, including acute and chronic pulmonary diseases. We have previously described the molecular, structural, and functional aspects of the development of chronic lung injury and pulmonary fibrosis caused by intratracheal instillation of HCl in mice. Although mouse models of human disease have many advantages, rodents are evolutionary far from human and exhibit significant anatomical and physiological differences. Genetic and anatomic similarities between rabbits and humans are significantly higher. Rabbit models of HCl-induced lung injury have been used sparsely to evaluate acute lung injury. In this study, for the first time, we utilized rabbits as a model of HCl-induced pulmonary fibrosis and chronic lung injury. We present molecular, histological, and functional evidence that demonstrate the utility of using this model for studying new pharmaceutics against pulmonary fibrosis.