DC motors are widely used as propulsions, including in electric bicycles. The problem faced by students in the DC motor control laboratory working using software simulation is that they do not have practical learning experience using digital instruments. This article aims to develop a DC motor speed control that can be used to learn practical Proportional Integral Derivative (PID) control in the laboratory. The DC motor speed control was developed using a combination of Arduino UNO microcontroller and Matlab software. The PID method was used because it is still broadly studied and applied in industries. The test results showed that the developed trainer can work well with PID variable values that can be entered via the keypad, and DC motor transient responses can be displayed in Matlab. From the experimental results, it was found that the optimal PID variable values were Kp=0.04, Ki=0.05, and Kd=0.004, where the controller produced a low overshoot value, i.e., 0.73% of its set point and a settling time of 10.66 seconds. The test results of using the developed trainer in the Fundamental of Control Engineering laboratory work showed that the developed trainer gave students practical learning experience in designing PID control for DC motor speed by using digital equipment, i.e., microcontroller and actual DC motor as well as analyzing its corresponding transient response in Matlab software environment