Serum Omentin-1 Levels Research Articles

Serum Omentin-1 Levels in Patients with Type 2 Diabetes Mellitus and their Correlation with Lipid Profile: A Case-control Study

Introduction: Type 2 Diabetes Mellitus (T2DM) is characterised by a variable degree of insulin resistance, leading to hyperglycemia. Serum omentin-1, an important adipocytokine, plays a role in maintaining tissue sensitivity to insulin and lipid metabolism. Therefore, circulating omentin-1 can serve as a biomarker for obesity, diabetes, and dyslipidemia. Aim: The aim of this study was to compare serum omentin-1 levels between type 2 diabetic cases and non diabetic controls, and to investigate the correlation of omentin-1 levels with the lipid profile. Materials and Methods: A hospital-based case-control study was conducted at Government Medical College, Trivandrum, from December 2017 to November 2018. The study included 30 T2DM patients and 30 non diabetic controls. Serum omentin-1 levels, fasting blood sugar, glycated haemoglobin (HbA1C), and serum lipid profile were measured in all study subjects. Statistical analysis included Student’s t-test and Pearson’s correlation coefficient to assess the correlation between variables. Chi-square test was performed to analyse age and sex distribution among the study groups. Results: The mean age of diabetic cases was 56.7±7.9 years, while the mean age of non diabetic controls was 52.9±5.5 years. Among the subjects, 43% of diabetics and 50% of non diabetics were male, while 57% of diabetics and 50% of non diabetics were female. The mean value of serum omentin-1 levels was significantly lower in the diabetic population (273.37±60.18 ng/mL) compared to the non diabetic controls (573.4±51.6 ng/mL) with a p-value of <0.001. Serum omentin-1 showed a significantly strong negative correlation with total cholesterol, low-density lipoproteins (LDL), and triglycerides, and a positive correlation with high-density lipoproteins (HDL). Conclusion: In the present study, serum omentin-1 levels were significantly lower in the diabetic population, and their lipid profile exhibited abnormal values. This suggests that omentin promotes insulin sensitivity and affects lipid metabolism, thus preventing insulin resistance and dyslipidemia in T2DM patients. Therefore, omentin may play a role in reducing cardiovascular complications.

Study of the relation between serum omentin-1 levels and insulin resistance in gestational diabetes mellitus

Gestational diabetes mellitus (GDM) is defined as carbohydrate intolerance of variable degree that begins or is first identified during pregnancy. Pregnant women with GDM have an increased risk of adverse metabolic outcomes. It is also associated with a higher likelihood of developing obesity and type 2 diabetes mellitus (T2DM) in a mother and her child. Omentin-1, a novel anti-inflammatory adipokine, is recognized as a main visceral secretory adipokine that may play a role in the context of both glucose homeostasis and insulin resistance (IR). Objective. To evaluate serum omentin-1 levels in GDM and its relationship with glycemic control, IR, and metabolic parameters. Patients and methods. This study included 75 patients who were divided into three groups: group I – 25 pregnant women with GDM diagnosed according to the WHO-2013 criteria, group II – 25 pregnant women with normal glucose tolerance, group III (control) – 25 healthy non-pregnant women. Fasting plasma glucose (FPG) levels, fasting plasma insulin (FPI) levels, fasting lipid profile, omentin-1, and HOMA-IR were measured in all patients, and a 2-hour 75-g oral glucose tolerance test (OGTT) was performed. Results. Between the groups of patients with and without IR, there was a statistically significant difference regarding serum omentin-1 levels, FPG, FPI, HOMA-IR, triglyceride (TG) levels, total cholesterol levels, as well as high-density lipoprotein (HDL) and low-density lipoprotein (LDL) levels (p < 0.01). Serum omentin-1 levels had a negative association with FPI and HOMA-IR in all study groups. In addition, there was a strong negative correlation between serum omentin-1 and glycemic control, total cholesterol, TG, and LDL (p < 0.01) and a strong positive correlation between omentin-1 and HDL (p < 0.01) in all study groups. Conclusion. There was a strong negative correlation between omentin-1 and FPI levels, HOMA-IR, glycemic control, total cholesterol, TG, and LDL in all study groups. Moreover, serum omentin-1 levels were positively correlated with HDL. This suggests that omentin-1 has an essential role in IR during pregnancy. Key words: gestational diabetes mellitus, insulin resistance, omentin-1

Evaluation of serum adipokines (omentin-1 and visfatin) in coronary artery disease at a North Indian hospital.

Objective. Adipose tissue is considered to be an endocrine organ that secretes bioactive substances known as adipokines that contribute to the pathophysiology of metabolic and coronary diseases related to obesity. In this study, various novel biomarkers, such as inflammatory markers that are pro-inflammatory (visfatin) and anti-inflammatory (omentin-1), as prognostic indicators for people with coronary artery disease (CAD) were investigated. Methods. In this study, 30 diabetic patients with CAD, 30 diabetic patients without CAD, and 30 healthy control counterparts were included. Serum omentin and visfatin concentrations were evaluated by solid-phase enzyme linked immunosorbent assay (ELISA) kit. Patients with established diagnosis of CAD based on angiography, ECG, and elevated cardiac marker level were included into the study. Patients with cardioembolic stroke, cerebral venous sinus thrombosis, CNS vasculitis, and hemorrhage due to trauma, tumor, vascular malformation, and coagulopathy were excluded. Results. The serum omentin-1 levels were significantly higher in the healthy controls in comparison with the diabetic group (p<0.0001) and serum visfatin levels were significantly higher in the diabetic group in comparison with the healthy controls (p<0.0001). The serum omentin levels were significantly higher in the diabetic group in comparison with the cardio-diabetic group (p<0.0001) and serum visfatin levels were significantly higher in the cardio-diabetic group in comparison with the diabetic group (p<0.0001). The serum omentin-1 showed negative correlation with the serum visfatin in the cardio-diabetic group. Conclusion. The adipokines, such as omentin and visfatin, may be good therapeutic candidates in preventing or ameliorating CAD.

Assessment of Serum Omentin-1, Adipsin and Resistin Levels in Beta Thalassemia Male Patients in Baghdad City of Iraq

Adipocytokines has a significant impact on the development of many illnesses. (e.g. beta thalassemia), This research aims to determine the levels of Omentin-1, adipsin and resistin of (35) male patients their age (20–30) years that effected with Beta-Thalassemia city and (15) of healthy people as control group in Baghdad city by using enzyme linked immuonsorbant assay (ELISA). The findings of this research revealed a significant increase (p ≤ 0.01) in levels omentin-1, which are (78.33 ± 6.14) pg/mL in patients group and (35.88 ± 5.8) ) pg/mL in control group. The levels of adipsin show a significant increase (p ≤ 0.01), which are (603.13 ± 11.46) ng/mL in patients group and (171.76 ± 17.45) ng/mL in patients group in control group, also a significant increase (p ≤ 0.01) in the levels of resistin which are (2.1 ± 0.5) ng/mL and (0.35 ± 0.06) ng/mL in control group.

Omentin as an Independent Predictor of Metabolic Syndrome and Obesity Among Adolescents in Northeast China.

We investigated the association of omentin with metabolic syndrome (MetS), MetS components, and obesity in adolescents. A total of 742 middle-school students from Liaoyang City were enrolled in this cross-sectional study using the stratified cluster sampling method. Clinical information and blood samples were collected, and serum omentin levels were measured using enzyme-linked immunosorbent assay. Mean plasma omentin levels were lower in male than in female participants (88.25 (interquartile range 63.02-133.61) vs 99.46 (interquartile range 69.08-188.35) ng/L, P = 0.004). The participants were divided into four groups according to the quartile (Q) values of omentin from low to high. With increasing omentin levels from Q1 to Q4, the age of adolescents and the proportion of males gradually increased (P < 0.05), whereas the body mass index (BMI) (P < 0.05) and prevalence of MetS (P > 0.05) tended to decrease. Omentin levels were significantly and negatively correlated with waist circumference and BMI (correlation coefficients of -0.099 and -0.115, respectively). Regression analysis showed that omentin level was independently associated with the risk of MetS (Odds ratio, OR = 0.639, 95% confidence interval, CI (0.432, 0.945)), which was attributed to the association with central obesity (OR = 0.775, 95% CI (0.605, 0.993)) among MetS components. Increased omentin levels also indicated a reduced risk of obesity (OR = 0.700, 95% CI (0.563, 0.870)). Omentin is an independent predictor of MetS and obesity among adolescents in northeast China.

Are levels of adipokines and micronutrients different in male adult smokers and non-smokers? A case–control study

ObjectiveSmoking is a common public problem leading to increases in oxidative stress and decreases in the levels of some micronutrients, finally affecting adipokine levels. The aim of this study was to compare the serum levels of omentin (intelectin-1), chemerin, TNF-α, and some micronutrient intakes in male smokers and non-smokers. Methods40 male smokers and 40 male non-smokers with a mean age of 38.6±14.1 years were included in this study. Serum levels of omentin, chemerin, and TNF-α were measured. To calculate the daily intake of energy, carbohydrate, protein, fat, and some of the micronutrients, the 24-h recall and semi-quantitative food frequency questionnaire (FFQ) was used. ResultsOmentin, chemerin, and TNF-α levels in male smokers were lower than non-smokers, but these differences were not statistically significant. However, after adjustment for total and saturated fat intakes and age, omentin (β=138.4, p=0.027) and TNF-α (β=144.5, p=0.015) revealed significant differences. ConclusionThe serum levels of omentin, chemerin, TNF-α, and some micronutrient intakes were not significantly different between smokers and non-smokers. Further population studies are needed to clarify this subject.

Estimation of Serum Level of Omentin-1 In Hirsute Female: A Single Center Study

Estimation of Serum Level of Omentin-1 In Hirsute Female: A Single Center Study

Diagnostic and Prognostic Role of Serum Omentin and NGAL Levels in Egyptian Breast Cancer Patients.

Background Breast cancer (BC) is globally the main cause of cancer-related deaths in women. Tumor biomarkers have significant role in diagnosis and predicting the prognosis and decide the specific therapy to each patient. Aim In this study, we investigated whether omentin and NGAL levels were altered in patients with breast cancer and the relationship between these markers and their clinicopathological parameters. Subjects and Methods. This study included 120 patients with breast cancer and 30 healthy individuals served as controls. We measured the serum level of omentin and NGAL by ELISA technique. Results Our results showed that there were statistically significant differences in serum omentin and NGAL levels between two groups. Also, in breast cancer patients, there was significant difference between omentin level, the same results with NGAL level and patient's age, tumor size, lymph node, and metastasis. No significant relationship was found between omentin level and tumor grade, ER, PR, and HER2. The cutoff value for the prediction of breast cancer was determined at >113.2 ng/ml for omentin and >145.3 ng/ml for NGAL with a sensitivity of 91.7% and 100%, specificity of 100% and 80%, positive predictive value of 100% and 90.9%, negative predictive value of 85.7% and 100%, and accuracy of 94.4% and 93.3%, respectively. In conclusion, serum omentin and NGAL can be used as strong diagnostic markers for breast cancer.

Association between Circulating Omentin-1 Levels and Aortic Valve Sclerosis.

Aortic valve sclerosis (AVS) is characterized by thickening of the valve leaflets accompanied by increased echogenicity and calcification without significant limitations in valve movements. Omentin-1 is a glycoprotein of the adiponectin family released from visceral adipose tissue, and it can be used as a biomarker of atherosclerosis, obesity, and metabolic syndrome. No studies have demonstrated any relationship between AVS and omentin-1 in the literature. We aimed to explore the association of serum omentin-1 levels with AVS. Eighty-six patients with AVS and 92 age- and sex-matched controls were enrolled into the study. The baseline clinical characteristics of the patients were recorded. Conventional 2-dimensional echocardiography was performed. Omentin-1 levels were measured. The mean omentin-1 level was significantly lower in the AVS (+) group compared to the control group (78.16 ± 44.95 vs. 163.57 ± 59.84 ng/mL, p < 0.001). Omentin-1 [odds ratio (OR) = 3.45, 95% confidence interval (CI) = 1.88-5.39, p < 0.001,] and LDL-C (OR = 1.82, 95% CI = 1.33-2.16, p = 0.015) were found to be independent predictors of AVS in multivariate logistic regression analysis. An omentin-1 level of < 92.45 ng/mL had 90.5% sensitivity and 71.4% specificity for the prediction of AVS (area under curve: 0.697, p < 0.001). Our results indicated that a lower omentin-1 level was associated with an increased risk of AVS. We suggest that omentin-1 could be used as a treatment target as well as to predict AVS.

Expression of Serum Omentin, CTRP9, and Vaspin in Patients with Polycystic Ovary Syndrome.

Objective To explore the relationship between serum omentin, C1q/tumor necrosis factor-related protein-9 (CTRP9), and visceral fat-specific serine protease inhibitor (vaspin) levels in different phenotypes in patients with polycystic ovary syndrome (PCOS). Methods One hundred PCOS patients treated at our hospital's clinic of reproductive medicine were chosen and included into the research group, and 100 healthy women who came for physical examination during the same time period were included into the control group. According to the definition of obesity by the WHO (body mass index (BMI) ≥25 kg/m2), 100 patients with PCOS were equally divided into obese (study group A) and nonobese (study group B) groups. 100 healthy women were also divided into obese (control group A) and nonobese (control group B) groups with 50 patients each. Comparison among the 4 groups was performed in factors/indicators including the serum omentin, CTRP9, and vaspin levels and biochemical indexes (triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), fasting insulin (FINS), total testosterone, and homeostasis model assessment of insulin resistance (HOMA-IR) levels), and the correlation analysis was conducted with omentin, CTRP9, and vaspin. Results There was no significant difference in age, TG, TC, and LDL-C among the 4 groups (P > 0.05). The BMI, WHR, HDL-C, and omentin of the obese phenotype were significantly different from those of the nonobese phenotype (P < 0.05). Among the four groups, FINS, HOMA-IR, and vaspin in group A (obesity) was the highest, and the control group B (nonobese) was the lowest. There was no significant difference in the levels of study group B (nonobese) and control group A (obesity). The level of CTRP9 in the study group was significantly lower than that in the control group (P < 0.05). Taking serum omentin, CTRP9, and vaspin levels of patients in the study group as dependent variables, Pearson correlation analysis showed that the omentin level was negatively correlated with BMI, WHR, FINS, TG, TC, LDL-C, HOMA-IR, and TT levels (P < 0.05) and was positively correlated with the HDL-C level (P < 0.05); CTRP9 level was negatively correlated with BMI, TC, and HOMA-IR (P < 0.05) and was not correlated with age, WHR, FINS, TG, HDL-C, LDL-C, HOMA-IR, and TT levels. The vaspin level was positively correlated with BMI, WHR, FINS, TG, TC, LDL-C, HOMA-IR, and TT levels (P < 0.05) and negatively correlated with HDL-C levels (P < 0.05) and was not correlated with age. Conclusion When compared with healthy people, PCOS patients have higher serum vaspin levels and lower CTRP9 levels; BMI, TC, LDL-C, FINS, TG, total testosterone, HDL-C levels, waist-to-hip ratio, and HOMA-IR are all closely related to serum vaspin and CTRP9 levels; increasing serum CTRP9 levels and decreasing vaspin levels help to slow progress and promote prognosis of the disease. Serum omentin level is connected with the obesity index but not with PCOS.

Relationship of omentin-1 with oxidative stress in Iraqi patients with myocardial infraction

A recently recognized adipokine mediator omentin-1 mainly expressed in human epicardial adipose tissue (EAT) has been found to inhibit the inflammatory response and atherosclerosis factors that has potential beneficial effects on cardiovascular disorders. Recent clinical studies reported that the serum omentin-1 levels were negatively related to the severity of Ischemic heart diseases. In this study, we characterized the clinical significance of omentin-1 in myocardial infarction (MI) patients.Investigated its association with oxidative stress, and demonstrated its protective effect as an antioxidant. The study contained 120 subjects who were divided evenly into patients and control groups for the period between September and December 2021. The serum levels of omentin-1 were assessed by enzyme-linked immunosorbent assays methods and serum zinc and copper were measured using atomic absorption spectroscopy. Also, protein oxidation markers and malondialdehyde (MDA) serum levels were measured by spectrophotometer.

Effect of 4 Weeks of Progressive Resistance Training on Plasma Levels of Omentin-1 and Metabolic Factors in Diabetic Male Rats

Background and Objectives Omentin-1 is a new adipokine with a potential to increase insulin sensitivity, and is associated with lipid profile. There are limited studies on the effect of exercise on the serum level of Omentin-1. The present study aims to investigate the effect of progressive resistance training (PRT) on plasma level of omentin-1 and glycemic control in diabetic male rats. Subjects and Methods In this study, 24 male Wistar rats were randomly divided into three groups: Control, diabetic, and diabetic+PRT. The training group performed a PRT program using a ladder (three days a week, for 4 weeks). Body weight, glucose level, amentin-1 level, insulin level, and lipid profile were measured. Results The PRT significantly increased plasma levels of omentin-1 and triglycerides in the diabetic + PRT group compared to the diabetic group (P=0.017). There was no significant difference in high-density lipoprotein (F=0.318, P&gt;0.05), low-density lipoprotein (F=0.612, P&gt;0.05) and cholesterol (F=0.049, P&gt;0.05) levels between the study groups. Conclusion The PRT improves omentin-1 and insulin resistance in diabetic rats.

Association between Serum Omentin-1 Level and Insulin Resistance, Hematological Factors and Gestational Age in Preterm Newborn

Association between Serum Omentin-1 Level and Insulin Resistance, Hematological Factors and Gestational Age in Preterm Newborn

Sortilin/Omentin-1 ratio in peripheral artery disease: A cross-sectional study on 295 unselected elderly patients

BackgroundThe role of sortilin and omentin-1 in the pathogenesis of atherosclerosis and vascular disease is an emerging topic in recent years. These molecules can be found circulating in the blood. Recent studies have shown how these biomarkers appear to correlate with the severity of PAD. The levels of these molecules appear to be inversely proportional to each other. Their relationship may provide further insight into the management of the very old diabetic patients with PAD. This study aimed to assess the possible role of sortilin/omentin-1 ratio as easy-to-measure marker in peripheral artery disease (PAD) in type-2 diabetic patients. MethodsThis study analyzed the association between sortilin and omentin-1 serum levels and the presence of clinically significant lower limb PAD in diabetic individuals. We enrolled 295 diabetic patients, including 179 with PAD. Serum levels were collected and correlated with clinical characteristics of the patients. ResultsSortilin concentration was significantly higher in the latter group compared to the former and there was a trend toward increased sortilin levels as disease severity increased. Omentin-1 serum levels were significantly lower in diabetic patients with PAD than in diabetic controls and the levels gradually decreased in proportion to disease severity. The ratio of sortilin to omentin-1 was significantly higher in patients with PAD compared to the other group. ConclusionThe sortilin to omentin-1 ratio appears to be a predictive factor for PAD in patients with type-2 diabetes and it may be a promising marker for clinically significant atherosclerosis of the lower limbs. Further studies are needed to confirm this finding and to evaluate its clinical usefulness.

A Randomized Trial with Two Hypocaloric Diets with Different Lipid Profiles and Effects on Serum Omentin-1 Levels in Obese Subjects

Background The effects of weight loss therapies on omentin-1 levels have been unclear, showing both elevations and decreases in circulating levels. The role of dietary fat might have an important role. The aim of our investigation was to evaluate the influence of weight decrease on omentin-1 levels after two different high-fat hypocaloric diets. Methods 319 Caucasian obese subjects were randomly allocated during 12 weeks (Diet M (high monounsaturated fat diet) vs. Diet P (high polyunsaturated fat diet)). The mean age was 47.2 ± 5.0 years (range: 26-64), and the mean body mass index (BMI) was 37.9 ± 4.1 kg/m2 (range: 30.6-39.8). Sex distribution was 237 females (74.7%) and 72 males (25.3%). Anthropometric and biochemical parameters were evaluated at basal and after both diets. SPSS 23.0 has been used to realize univariant and multivariant statistical analysis. Results After both diets, BMI, weight, fat mass, waist circumference, systolic blood, LDL-cholesterol, insulin levels, and HOMA-IR decreased in a statistical way from basal values. These improvements were similar in both diets. After Diet P, omentin-1 levels increase (21.2 ± 9.1 ng/ml: P = 0.02), and after Diet M, this adipokine increases (47.1 ± 11.2 ng/ml: P = 0.02), too. The increase of omentin-1 with Diet M was statistically significantly higher than that after Diet P (P = 0.01). A multiple regression analyses adjusted by age and sex reported a statistical relation between BMI (kg/m2) and insulin (UI/L) with omentin-1 levels. Conclusions Our study demonstrated a significant improvement on serum omentin-1 levels after weight loss secondary to both diets; in contrast, omentin-1 improvement was higher with Diet M than with Diet P.

Analysing adipokine Omentin-1 in periodontal disease and type-2 diabetes mellitus: An interventional comparative study

Background and objectiveOmentin-1 is an anti-inflammatory adipokine associated with metabolic disorders including insulin resistance, glucose intolerance and type 2 diabetes mellitus (T2DM). Diabetes is a crucial systemic risk factor for occurrence of periodontitis. Periodontitis is deliberated as a complication of diabetes. This study aimed to estimate salivary and serum levels of Omentin-1 in chronic periodontitis with or without diabetes mellitus before and after scaling and root planing (SRP) and also the level of glycemic control. Materials and methodsBased on history and clinical parameters, 70 subjects were allocated into two groups i.e. chronic periodontitis (Group 1) and chronic periodontitis with well controlled T2DM (Group 2). Baseline saliva and serum samples were collected from both groups followed by full mouth SRP. Subjects were recalled 3 months post SRP for salivary and serum sample collection and measurement of clinical parameters. Serum as well as salivary samples collected were further analysed using enzyme-linked immunosorbent assay to determine the Omentin-1 concentration in each sample. ResultsThe levels of Omentin-1 in saliva and serum were significantly lower in both the groups at baseline. Clinical parameters exhibited a significant reduction 3 months post SRP in both groups. Salivary and serum Omentin-1 levels significantly increased in both the groups, 3 months post SRP. HbA1c levels also reduced significantly post therapy. ConclusionA significant relation exists between salivary and serum Omentin-1 in chronic periodontitis and chronic periodontitis with T2DM. In consequence, Omentin-1 level can have possible application as inflammatory marker of T2DM, periodontal disease and treatment outcome.

Analysis of Changes in Serum Levels and Gene Expression Profiles of Novel Adipocytokines (Omentin, Vaspin, Irisin and Visfatin) and Their Correlation with Serum C-reactive Protein Levels in Women Diagnosed with Endometriosis

This study aimed to investigate the role of new adipocytokines (omentin, vaspin, irisin and visfatin) in the development of endometriosis and the relationship of these adipocytokines with the inflammatory marker, C-reactive protein (CRP) levels in serum. In this study, endometriosis (n=16) and control groups (n=14) were determined via ultrasound. Serum omentin, vaspin and irisin levels were measured by ELISA method. CRP levels in serum and the gene expression of visfatin and vaspin in whole blood samples were determined by clinical analyzer and the real-time polymerase chain reaction, respectively. Serum irisin and CRP levels in the endometriosis group were significantly higher than in the control group. Irisin protein levels demonstrated a positive correlation with body mass index and CRP in women diagnosed with endometriosis. No statistically significant difference was found in serum omentin and vaspin levels between groups. The visfatin and vaspin gene expression in whole blood samples from the endometriosis group was found to be significantly lower than the control group. Increased levels of serum irisin and decreased visfatin and vaspin gene expressions in blood may be considered as a potential biomarker in endometriosis. The identification of new adipocytokines, which demonstrate an alteration in the presence of endometriosis and the relationship between these adipocytokines and inflammation will facilitate the detection of mechanisms involved in endometriosis and will lead to the development of targeted therapy.

Association of promoter region A-1012G polymorphism (rs4516035) of vitamin-D receptor gene with coronary artery disease.

To investigate the correlation of Adenine-1012-Guanine (rs4516035) promoter region polymorphism of vitamin-D receptor gene with serum levels of omentin-1, vitamin-D and vitamin-D receptor protein in patients with coronary artery disease. The case-control study was conducted from January to June 2020 at the cardiac unit of Civil hospital Karachi (CHK), and comprised coronary artery disease patients and controls. The tetra-primer amplification refractory mutation system polymerase chain reaction method was used to genotype Adenine-1210Guanine polymorphism in the vitamin D receptor gene. Serum levels of omentin-1, vitamin-D, and vitamin-D receptor protein were measured in both the groups using an enzyme-linked immunosorbent assay. Data was analysed using SPSS 17. Of the 1,000 subjects, there were 500(50%) cases; males 306(61.2%) and 194(38.8%) females with overall mean age of 51.08±9.55 years. The remaining 500(50%) were controls; 290(58%) males and 210(42%) females with overall mean age of 50.9±10.78 years. The mutant Guanine allele was more prevalent in controls 261(52.2%), and had a non-significant correlation with coronary artery disease (p=0.45). Among the cases, the wild Adenine-Adenine genotype had a higher prevalence 402(80.4%) and had a significant correlation with coronary artery disease (p<0.001). The heterozygous genotype Adenine-Guanine was significantly more predominant among the controls 346(69.2%) compared to the cases 66(13.2) (p=0.002). Adenine-1012-Guanine polymorphism in the vitamin-D receptor gene was found to be a protective polymorphism for coronary artery disease in the recessive model.

Association of Omentin rs2274907 Gene Polymorphism and Its Protein on the Risk of Obesity and Metabolic Disorder in Thai Children

Omentin is an adipocytokine that expresses in white adipose tissues. It is a marker for preventing the development of pro-inflammatory states related to diseases like obesity. This study aimed to examine the concentrations of omentin and anthropometric-biochemical parameters in obese children and healthy controls. Also, to search for the associations of omentin rs2274907 gene polymorphism with omentin levels and obesity among Thai children. A total of 293 children, 178 obese cases and 115 healthy controls, were recruited for this study. The omentin level was measured using the enzyme- linked immunosorbent assay (ELISA), and genotyping was performed using the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method. Serum omentin levels were significantly lower in the obese group than in the control group (13.9 and 25.0 ng/mL, respectively, p &lt; 0.001). The omentin levels had significant negative correlation with body weight, body mass index (BMI) percentile, waist circumference, triglycerides (r = –0.276, –0.275, –0.259, –0.242, p &lt; 0.001), and had positive correlation with high-density lipoprotein cholesterol (HDL-C) levels (r = 0.210, p &lt; 0.001). There was a significant association between obesity and serum omentin levels (p &lt; 0.05). However, the omentin rs2274907 gene polymorphism showed no significant relationship with the omentin levels and obesity among Thai children. These findings in Thai children suggest that omentin level, but not omentin rs2274907 gene polymorphism, may influence susceptibility to obesity.

Association of vaspin rs2236242 and Val109Asp omentin genes polymorphism and their serum levels with increased risk of breast cancer

Breast cancer represents the leading cause of cancer-related death in women globally. Obesity is one of the risk factors for breast cancer, particularly in postmenopausal women. Adiposopathy causes hypertrophy, which promotes metabolic dysregulation, hypoxia, inflammation, insulin resistance, and altered adipokine production such as vaspin and omentin-1. The objective of this study is to inspect the link between the vaspin rs2236242 and the Val109Asp omentin gene polymorphisms and their serum levels with breast cancer occurrence in postmenopausal females. The gene polymorphisms of vaspin rs2236242 and Val109Asp omentin were identified by T-ARMS-PCR and PCR-RFLP respectively. The glucose homeostasis traits, lipid profile, total antioxidant capacity, and circulating vaspin and omentin-1 levels were all assessed. The AT genotype of vaspin rs2236242 and the Val/Asp genotype of Val109Asp omentin were more prominent in breast cancer patients than the TT and Asp/Asp genotypes respectively. Breast cancer patients showed significant lower levels of serum vaspin and omentin-1. The AT genotype of the vaspin rs2236242 polymorphism, the heterozygous Asp/Val genotype of the omentin-1 gene, and low serum vaspin and omentin-1 levels are more associated with breast cancer incidence. On the flip side, higher levels of serum vaspin and omentin appear to protect against the incidence of breast cancer.