Serrulata Var Research Articles

Antioxidant and tyrosinase inhibitory activities of different parts of oriental cherry (Prunus serrulata var. spontanea)

Six different parts (branch, flesh, flower, fruit, leaf, and seed) of oriental cherry (Prunus serrulata var. spontanea) were extracted with ethanol or water, then total phenol content (TPC), antioxidant activity, and tyrosinase inhibitory activity of the extracts were evaluated. The ethanol extracts showed higher TPC and antioxidant activity than the water extracts regardless of parts. The ethanol extracts of leaf as well as branch possessed superior TPC and antioxidant activity. The highest tyrosinase inhibitory activity was found in ethanol extract of leaf. There was no dramatic difference of tyrosinase inhibitory activities according to parts of cherry. The results suggest that leaf and branch of oriental cherry could be a candidate for antioxidant and anti-whitening materials in food or cosmetic industries.

Temporal variation in different life stages of moths (Lepidoptera) from a southwestern temperate forest of Korea: A preliminary study

Two study sites in a temperate forest in Mt. Seungdal-san, Muan, Jeonnam in southwestern Korea were selected for the collection of caterpillars and adult moths (Lepidoptera). Here, we determined (1) the characteristics of moth fauna based on lepidopteran larvae and adults, and (2) the differences in temporal variation between these two developmental stages. In addition, we investigated the life history of moth species collected at the surveyed sites. Of the 206 caterpillars collected, 17 moth species from 9 families were successfully reared. A total of 342 adult moth species and 2108 individuals from 15 families were collected. Twenty-two plants were identified as host plants of 188 caterpillars. Two tree species, Prunus serrulata var. spontanea and Quercus serrata, were widely distributed in the study sites. Leaf water content (%) of Quercus serrata and Carpinus laxiflorawas the highest in June, whereas the leaf water content of Platycarya strobilacea was the highest in August. We postulated that many of the variables in the temperate forest, such as host plant availability, parasitoids, disease, and weather, affected the lepidopteran caterpillar populations. There were differences in species composition among the sampling methods which suggest the need for diverse methods to uncover total biodiversity. However, it should be noted that a long term and well-planned study with efficient rearing of caterpillars is needed to better understand these correlations.

Study on Nitrogen·Phosporus Absorption and Growth of Seedling of Prunus serrulata var. serrulata f. spontanea(E.H. Wilson) C. S. Chang by Treatment with Dried Swine Excrement

Prunus serrulata var. serrulata f. spontanea (E. H. Wilson) C. S. Chang was treated with dried swine excrement at various concentration levels, and their growth reactions and the contents of total kjeldahl nitrogen and total phosphoric acid were analyzed. The result is as follows; 1. When the plants were treated with 0.25% of dried swine excrement, the seed germination rate of Prunus serrulata var. serrulata f. spontanea was higher than that of the control. However, it showed a tendency to decrease when the treatment concentration got higher than 0.25%. 2. The growth rate of species was highest when they were treated with 0.25% of dried swine excrement. It tended to decrease with higher treatment concentration than 0.25%. Significant difference was clear between the control and the experimental plants of Prunus serrulata var. serrulata f. spontanea. 3. The contents of nitrogen and phosphoric acid in Prunus serrulata var. serrulata f. spontanea also got higher as the concentration of dried swine excrement increased. However, the content of total kjeldahl nitrogen was highest in the leaves, and followed by the roots and stems. In contrast, the content of total phosphoric acid were highest in the roots, and followed by the leaves and stems. Therefore, stems had the lowest contents of both nitrogen and phosphoric acid. 4. The contents of total kjeldahl nitrogen and total phosphoric acid in the soil were measured before and after the growth experiment of Prunus serrulata var. serrulata f. spontanea treated with dried swine excrement. The contents of nitrogen and phosphoric acid significantly decreased after the experiment. In conclusion, the contents of total kjeldahl nitrogen and total phosphoric acid accumulated in the plants increased as the concentration level of dried swine excrement got higher. The seed germination and plant growth rates were best at 0.25% treatment of dried swine excrement. The treatment of dried swine excrement may bring high effects on increasing the plant growth rate but could damage the plants with higher concentration than they need. Different optimal concentration levels of dried swine excrement for different plants should be found before it is used as fertilizer.

Marine Algae Collected during the Scientific Expedition to Bachok, Kelantan and the Islands of Terengganu with One New Record, Pterocladiella for Malaysia

In 2008, a scientific expedition to Bachok (the new Marine Station), Kelantan and the vicinity of Terengganu, was organised by the Institute of Ocean and Earth Sciences (IOES), University of Malaya. The objective of the expedition was to collect and document the natural resources of Bachok and its vicinity. Our team collected and identified the marine algae resources of the area. A total of 133 taxa of marine algae and four taxa of seagrasses were compiled from the results as well as previous records for Kelantan and Terengganu. The marine algae comprise 3 families of Cyanophyta with 4 genera and 4 taxa; 10 families of Chlorophyta with 13 genera and 35 taxa; 16 families of Rhodophyta with 38 genera and 63 taxa; 4 families of Phaeophyta with 8 genera and 31 taxa. Only 35 taxa were collected during this present survey, the most from the Rhodophyta (14 taxa) followed by the Phaeophyta (12 taxa), the Chlorophyta (8 taxa) and the Cyanophyta (4 taxa). Of these, the Cyanophyta Lyngbya, Oscillatoria, Microcoleus and Brachytrichia; the Chlorophyta Ulva compressa, Ulva flexuosa, Ulva intestinalis, Caulerpa serrulata var. boryana; the Rhodophyta Gelidiella acerosa, Gelidium pusillum, Actinotrichia fragilis, Acanthophora spicifera; and the Phaeophyta Dictyota ciliolata, Lobophora variegata, Padina antillarum, Hormophysa cuneiformis and Turbinaria conoides are new records for Kelantan and Terengganu. The Rhodophyta Pterocladiella is a new record for Malaysia. Two families of seagrasses with 3 genera and 4 taxa were collected and included in this checklist. A new species of Halophila will be studied further

시계열 분석을 이용한 부산지역 계절식물의 개화시기 변화

To examine the trend on the flowering time in some weather flora including Prunus serrulata var. spontanea, Cosmos bipinnatus, and Robinia pseudo-acacia in Busan, the changes in time series and rate of flowering time of plants were analyzed using the method of time series analysis. According to the correlation between the flowering time and the temperature, changing pattern of flowering time was very similar to the pattern of the temperature, and change rate was gradually risen up as time goes on. Especially, the change rate of flowering time in C. bipinnatus was 0.487 day/year and showed the highest value. In flowering date in 2007, the difference was one day between measurement value and prediction value in C. bipinnatus and R. pseudo-acacia, whereas the difference was 8 days in P. mume showing great difference compared to other plants. Flowering time was highly related with temperature of February and March in the weather flora except for P. mume, R. pseudo-acacia and C. bipinnatus. In most plants, flowering time was highly related with a daily average temperature. However, the correlation between flowering time and a daily minimum temperature was the highest in Rhododendron mucronulatum and P. persica, otherwise the correlation between flowering time and a daily maximum temperature was the highest in Pyrus sp.

Using urban effect corrected temperature data and a tree phenology model to project geographical shift of cherry flowering date in South Korea

Temperate zone deciduous tree phenology may be vulnerable to projected temperature change, and associated geographical impact is of concern to ecologists. Although many phenology models have been introduced to evaluate climate change impact, there has been little attempt to show the spatial variation across a geographical region due to contamination by the urban heat island (UHI) effect as well as the insufficient spatial resolution of temperature data. We present a practical method for assessing climate change impact on tree phenology at spatial scales sufficient to accommodate the UHI effect. A thermal time-based two-step phenological model was adapted to simulate and project flowering dates of Japanese cherry (Prunus serrulata var. spontanea) in South Korea under the changing climates. The model consists of two sequential periods: the rest period described by chilling requirements and the forcing period described by heating requirements. Daily maximum and minimum temperature are used to calculate daily chill units until a pre-determined chilling requirement for rest release is met. After the projected rest release date, daily heat units (growing degree days) are accumulated until a pre-determined heating requirement for flowering is achieved. Model parameters were derived from the observed bud-burst and flowering dates of cherry tree at the Seoul station of the Korea Meteorological Administration (KMA), along with daily temperature data for 1923–1948. The model was validated using the observed data at 18 locations across South Korea during 1955–2004 with a root mean square error of 5.1 days. This model was used to project flowering dates of Japanese cherry in South Korea from 1941 to 2100. Gridded data sets of daily maximum and minimum temperature with a 270 m grid spacing were prepared for the climatological normal years 1941–1970 and 1971–2000 based on observations at 56 KMA stations and a geospatial interpolation scheme for correcting urban heat island effect as well as elevation effect. We obtained a 25 km-resolution, 2011–2100 temperature projection data set covering peninsular Korea under the auspices of the Inter-governmental Panel on Climate Change—Special Report on Emission Scenarios A2 from the Meteorological Research Institute of KMA. The data set was converted to 270 m gridded data for the climatological years 2011–2040, 2041–2070 and 2071–2100. The phenology model was run by the gridded daily maximum and minimum temperature data sets, each representing climatological normal years for 1941–1970, 1971–2000, 2011–2040, 2041–2070, and 2071–2100. According to the model calculation, the spatially averaged flowering date for the 1971–2000 normal is earlier than that for 1941–1970 by 5.2 days. Compared with the current normal (1971–2000), flowering of Japanese cherry is expected to be earlier by 9, 21, and 29 days in the future normal years 2011–2040, 2041–2070, and 2071–2100, respectively. Southern coastal areas might experience springs with incomplete or even no flowering caused by insufficient chilling required for breaking bud dormancy.

Relationship between the Time and Duration of Flowering in Several Woody Plants in Springtime

To clarify the relationship between the timing and the duration of flowering among populations, plants, and individual flowers, the dates of flower budding, flowering and deflowering were monitored for ten woody species from March 1 to June 30, in 2005, 2006 and 2007, in temperate deciduous forests at three sites of Namsan, and individual plants from seven woody species were monitored from March 1 to May 31, in 2006. Total durations of flower budding, flowering, and deflowering varied among the plant species. Three durations of these phenological stages of Stephanandra incisa were the longest (74 days, 109 days, and 101 days, respectively), and those of Prunus serrulata var. spontanea were the shortest (7 days, 7 days, and 4 days, respectively). For each species, phenological durations varied among years but were similar among the study sites in the same year. There was no relationship between flowering time and flowering duration on the population level. On the plant level, the duration of flower budding was over 11 days in all specie; S. incisa had the longest duration (73.3 days), and that of Styrax japonica was long as well (29.0 days), while that of Prunus leveilleana was the shortest (11.3 days). The longer the mean flower budding duration, the greater the difference among the plants within a species. The flowering duration of for S. incisa was 92.2 days, while that of Forsythia koreana was 27.2 days. The flowering durations of all other species were <TEX>$10{\sim}20$</TEX> days. The deflowering duration was 92.0 days in S. incisa and <15 days in all other species. Differences among the plants in deflowering duration were smaller than those of the other phenological stages. In the species that flowered in April, the correlation coefficient between the flowering duration and the first flowering date was negative and significant. However, in the species that flowered in May, the correlation between flowering duration and the first flowering date was not significant. For individual plants of all species except for S. alnifolia, the earlier the flowering time, the longer the flowering duration. Differences between flowering time and flowering duration across years were significant in six species.

Characterization of wild Prunus yedoensis analyzed by inter-simple sequence repeat and chloroplast DNA

This study was initiated to attempt clarify the identities of taxa referred to as Prunus yedoensis that grows under natural environments in Jeju, Korea and of Yoshino cherry hybrids of cultivated origin (also recorded as P. × yedoensis) in Japan, and to understand the difference between these two taxa. P. yedoensis and other species collected from natural habitats from Jeju, Korea and cultivated materials of Yoshino cherries from Tokyo and Washington, DC, were analyzed with inter-simple sequence repeat (ISSR) markers, and sequence analysis of two chloroplast DNA (cpDNA) genes, rpl16 and trnL- trnF spacer. Depending on the source of Yoshino cherry, accessions show variations with ISSR and cpDNA. Accessions belonging to each of P. serrulata var. spontanea, P. serrulata var. pubescens, and P. sargentii were grouped closely to P. yedoensis and Yoshino cherry accessions. However, two Yoshino cherry accessions that include ‘Akebono’ showed the same rpl16 haplotype of A and A at the position of 113 and 206, respectively, which were found in 4 out of 16 P. yedoensis accessions. Twelve accessions of P. yedoensis and 11 other Yoshino cherries showed rpl16 haplotype of T and A at these positions. P. yedoensis native to Korea can be considered different from Yoshino cherry of hybrid origin from Japan based on ISSR markers and rpl16 haplotypes. Therefore, it may be concluded that the Korean taxon currently referred to as P. yedoensis can be considered indigenous and sufficiently distinct to warrant recognition as a distinct entity.

Antioxidative and Anticancer Activity of Extracts of Cherry (Prunus serrulata var. spontanea) Blossoms

Organic solvent (methanol, ethanol, and acetone) extracts and water extracts of cherry (Prunus serrulata var. spontanea) blossoms were prepared, and antioxidant activities of the extracts were evaluated. Methanolic CBE (100 microg/ml) showed the highest total phenol content (104.30 microM), radical scavenging activity (34.2%), and reducing power (0.391). The effect of CBE on DNA damage induced by H(2)O(2) in human leukocytes was evaluated by Comet assay. All CBE was a potent dose dependent inhibitor of DNA damage induced by 200 microM of H(2)O(2), methanolic CBE showed the most strong inhibition activity. The methanolic CBE of 500 microg/ml showed 38.8% inhibition against growth of human colon cancer cell line HT-29. These results indicated that cherry blossoms could provide valuable bioactive materials.

Reconsideration of the Prunus serrulata complex (Rosaceae) and related taxa in eastern Asia

The Prunus serrulata complex with several infraspecific taxa, including P. jamasakura and P. leveilleana, is indigenous to eastern Asia. Although these taxa are very common, the taxonomic confusion surrounding this complex is reflected in the ambiguity shown by the various taxonomic treatments currently used. Patterns of intraspecific variation and recognition of taxa within the P. serrulata complex and its related species were investigated in 468 individuals using principal components analysis. Particular emphasis was placed on P. serrulata, P. jamasakura, and P. leveilleana because of their high degree of morphological intergradation and inconsistent circumscription in the various systematic treatments. Although morphological variation among different taxa of the P. serrulata complex was continuous for most reproductive and leaf characters, the related taxa (P. pseudocerasus, P. lannesiana f. albida, and P. sargentii) were found to be distinct based on the number of flowers per inflorescence and peduncle length. In general, the number of flowers per inflorescence, the flower size, peduncle length, and hair density on the pedicel were found to be characters of great taxonomic value for most of the related taxa. However, there is not sufficient morphological evidence for the taxonomic splitting of P. serrulata to warrant the designation of varieties, with the exception of the pubescent taxon, P. serrulata var. pubesecens. © 2007 The Linnean Society of London, Botanical Journal of the Linnean Society, 2007, 154, 35–54.

Taxonomic diversity and geographic distributions of aquarium-traded species of Caulerpa (Chlorophyta: Caulerpaceae) in southern California, USA

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections MEPS 314:97-108 (2006) - doi:10.3354/meps314097 Taxonomic diversity and geographic distributions of aquarium-traded species of Caulerpa (Chlorophyta: Caulerpaceae) in southern California, USA Susan Frisch Zaleski1, Steven N. Murray2,* 1University of Southern California Sea Grant Program, University of Southern California, 3616 Trousdale Parkway, AHF 209, Los Angeles, California 90089-0373, USA 2California State University at Fullerton, College of Natural Sciences and Mathematics, PO Box 6850, Fullerton, California 92834-6850, USA *Corresponding author. Email: smurray@fullerton.edu ABSTRACT: We identified 14 taxa (10 species) of Caulerpa from retail aquarium outlets in southern California, USA, suggesting that the aquarium industry has the potential to be an important vector for transporting these non-native seaweeds into distant geographic regions. Seaweeds were sold in 58% and species of Caulerpa in 52% of the 50 stores visited between November 2000 and August 2001. C. serrulata var. hummii (18%), C. taxifolia (14%), C. racemosa (14%), and C. racemosa var. lamourouxii (14%) were the most commonly encountered taxa. The 14 aquarium-traded Caulerpa taxa were distributed from tropical to temperate waters. Only 3 (C. ashmeadii, C. serrulata var. hummii and C. microphysa) were confined to the tropics, whereas 12 had distributions extending into temperate seas. These temperate components to their distributions indicate that, if introduced, several other taxa of aquarium-traded Caulerpa besides C. taxifolia might be capable of establishing populations in southern Californian or other temperate waters. KEY WORDS: Aquarium industry · Caulerpa spp. · Geographic distribution · Invasive seaweeds · Marine invasions Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in MEPS Vol. 314. Online publication date: May 22, 2006 Print ISSN: 0171-8630; Online ISSN: 1616-1599 Copyright © 2006 Inter-Research.

A phenolic glucoside isolated fromPrunus serrulata var.spontanea and its peroxynitrite scavenging activity

A new phenolic glucoside (1), pursargentoside, was isolated from the leaves of Prunus serrulata var. spontanea, along with three other known compounds, orobol 7-omicron-glucoside (2), 1beta, 2alpha, 3alpha, 24-tetrahydroxy-urs-12-en-28-oic acid (3), and chlorogenic acid (4). The structure of pursargentoside (1) was identified by spectroscopic data analysis including 1D and 2D NMR spectroscopy, as 2-omicron-beta-(6'-benzoyl)-glucopyranosyl omicron-(Z)-coumaric acid. Compounds 1, 2, and 4 exhibited ONOO scavenging activity, whereas compound 3 was determined to be virtually inactive.

A New Pentacyclic Triterpenoid Glucoside from Prunus serrulata var. spontanea.

A new triterpenoid, 2alpha,3alpha,24-trihydroxyurs-12-en-28-oic acid-28-O-beta-D-glucopyranosyl ester (4) along with four known triterpenoids, ursolic acid (1), 2alpha-hydroxyursolic acid (2), 2alpha,3alpha,24-trihydroxyurs-12-en-28-oic acid (3), and 2alpha,3alpha,19alpha,24-tetrahydroxyurs-12-en-28-oic acid-28-O-beta-D-glucopyranosyl ester (5), were isolated from the leaves of Prunus serrulata var. spontanea (Rosaceae). Compounds 3-5 showed ONOO(-) scavenging activity, whereas compounds 1 and 2 were virtually inactive.

A new pentacyclic triterpenoid glucoside from Prunus serrulata var. spontanea.

A new triterpenoid, 2alpha,3alpha,24-trihydroxyurs-12-en-28-oic acid-28-O-beta-D-glucopyranosyl ester (4) along with four known triterpenoids, ursolic acid (1), 2alpha-hydroxyursolic acid (2), 2alpha,3alpha,24-trihydroxyurs-12-en-28-oic acid (3), and 2alpha,3alpha,19alpha,24-tetrahydroxyurs-12-en-28-oic acid-28-O-beta-D-glucopyranosyl ester (5), were isolated from the leaves of Prunus serrulata var. spontanea (Rosaceae). Compounds 3-5 showed ONOO(-) scavenging activity, whereas compounds 1 and 2 were virtually inactive.

밀양시 가로수 현황과 개선방안

The aim of this research is to survey the census of street trees and to provide remedial measures for street trees management to Miryang City. To do this, survey was carried out 16 region units in Miryang City. Through the survey, the current status of street trees’ map was made using automated map. From the results, it is turned out that 17,079 trees have been planted in Miryang City. The major species of street trees are Prunus serrulata var. spontanea(41.6%), Ginkgo biloba(26.1%), Pinus thunbergiana(13.3%) and the others are 12 species(19.0%). By the region units, Samrangjin-Eub is the most that 2,308 street trees are planted, and the next is Chodong-Myon(2,206 trees). The littlest street trees planted region is Cheongdo-Myon. In the results of comparison the component of street trees with other municipal cities, e.g. Seoul, Incheon, Kwangyang etc., only a few street trees have been planted so many in Miryang City. So, Miryang City indicated high proportionate street trees planting ratio. The suggested remedial measures are consisted with identifying the each street, increasing the green area by accepting the suitable street tree planting model, improving the physiological growth condition and systematic management with GIS.

In vitro antioxidant activity of some selected Prunus species in Korea.

In the course of the investigations of natural antioxidants, we examined the antioxidant activities of the methanol (MeOH) extracts of some selected Prunus species, including P buergeriana, P davidiana, P padus, P pendula for. ascendens, P. sargentii, P. serrulata var. spontanea and P. yedoensis by three methods as represented by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, total ROS (reactive oxygen species) and the peroxynitrite (ONOO-) scavenging activity tests. We also evaluated the activities of the organic solvent-soluble fractions, including the dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (n-BuOH) fractions and the water (H2O) layer of P serrulata var. spontanea leaves. By means of bioassay-directed fractionation, we isolated eleven known flavonoids (1-11) from the EtOAc soluble fraction of the MeOH extract of the Prunus serrulata var. spontanea leaves, exhibiting strong antioxidant activity and characterized as prunetin (1), genistein (2), quercetin (3), prunetin 4'-O-beta-glucopyranoside (4), kaempferol 3-O-alpha-arabinofuranoside (5), prunetin 5-O-beta-glucopyranoside (6), kaempferol 3-O-beta-xylopyranoside (7), genistin (8), kaempferol 3-O-beta-glucopyranoside (9), quercetin 3-O-beta-glucopyranoside (10) and kaempferol 3-O-beta-xylopyranosyl-(1-->2)-beta-glucopyranoside (11). Compounds 3 and 10 showed good activities in all tested model systems. Compounds 2 and 8 showed scavenging activities in the DPPH and ONOO- tests, while compounds 5, 7, 9 and 11 were active in the ONOO- and ROS tests. On the other hand, compounds 1, 4 and 6 did not show any activities in the tested model systems.

The Diversity and Availability of Caulerpa Species Found in Retail Aquarium Outlets in Southern California, USA

AbstractBiological invasions are recognized as a serious threat to marine biodiversity. Within the last 2 years, invasive populations of Caulerpa taxifolia were found in southern California's coastal waters as well as in New South Wales, Australia. In addition, two other seaweeds (Undaria pinnatifida and Caulacanthus ustulatus) also appear to have recently invaded southern California's warm temperate waters. The introduction of C. taxifolia has attracted much attention because this exotic seaweed is thought to have significantly altered the structure of Mediterranean marine ecosystems following its 1984 invasion. The southern California inoculation of C. taxifolia is believed to have resulted from the release of aquarium specimens. Besides C. taxifolia, other species of Caulerpa being sold for aquarium use also may have the potential to invade temperate waters. As a first step towards making this determination, the availability (% frequency) of Caulerpa species being sold in southern California for aquarium use was ascertained. Fifty retail saltwater aquarium stores were visited in three southern California counties between November 2000 and August 2001. Sixteen Caulerpa taxa were identified from the retail aquarium outlets. Caulerpa species were sold in 52% of these stores. C. taxifolia, ‘Mediterranean form’, was offered for sale in 10% of the visited stores; C. serrulata var. hummii (18%), C. racemosa (14%), and C. racemosa var. lamourouxii (14%) were the most commonly sold species. These data indicate that the aquarium industry is bringing into the region other species of Caulerpa besides C. taxifolia. Some of these species may also have the potential to invade temperate waters.