Background:- Syphilis generally considered to be a sexually transmitted disease, can also be transmitted in utero, and rarely by blood transfusion or non-sexual contact and is characterized by episodes of active disease, interrupted by latency. Congenital syphilis poses a significant challenge especially because infants may be still born, asymptomatic or present with a variable clinical picture at birth. Syphilis has been implicated in increasing susceptibility to HIV infection. We under took this study to determine the seroprevalence of syphilis in a tertiary care setting of Northern India and to identify an association with HIV-infection. Material & Method: - Of the total 19860 samples received during 5 years, VDRL was performed on 19860 samples and TPHA on 65 samples. Results: - 65 (0.33%) were found to be positive by VDRL. The patient’s positive for syphilis included 23 pregnant females (0.15% of the ANC samples received), 14(21.5%) HIV positive patients, 24 were from STD clinic (2.3% of the samples received from STD clinic). Conclusions: - The strong association between syphilis and HIV seroprevelence in this study suggests that acceleration of direct linkages between STI testing and HIV counseling and testing will be useful in enhancing the control of STI and HIV in India.