Sequence Of Auditory Stimuli Research Articles

Functional Organization of the Cerebral Cortex in Cued and Implicit Modality-Specific Anticipatory Attention. Analysis of α-Rhythm Coherence in the Sources Space

Studies in healthy right-handed subjects (N = 16, mean age 23 ± 5.7 years) analyzed the functional organization of the cerebral cortex during preparation to solve visual and auditory sensory tasks in two conditions: (1) anticipation of a visual or auditory signal after being told its modality (cued anticipatory attention) and (2) implicit anticipation formed during multiple repetition of a given sequence of visual and auditory stimuli. In both conditions subjects had to perform the same task – to identify the order of the stimuli (visual or auditory) in monomodal pairs. During the prestimulus period, the α frequency range was used to assess the coherence of cortical sources corresponding to previously selected cortical regions (regions of interest). More functional connections between cortical zones in the frontoparietal modulatory system were seen in cued anticipatory attention than during the period preceding appearance of the prompt stimulus, this obtaining in both sensory tasks. There was also a greater number of local connections between sensory-specific and associative (parietal and prefrontal) areas. Implicit anticipation preceding execution of the visual task was accompanied by an increase in connections between the ventral premotor cortex and the caudal (parietal and occipital) areas of the right hemisphere. Execution of the auditory task was preceded by an increase in connections between the auditory sensory cortex, the rostral part of the supplementary motor area, and the ventral premotor area. In contrast to cued attention, implicit anticipation was not accompanied by changes in frontoparietal connections. These results provide evidence of significant differences in the cerebral organization of these two types of anticipatory attention.

Temporal prediction restores the evaluative processing of delayed action feedback: an electrophysiological study.

The evaluative processing of the valence of action feedback is reflected by an event-related brain potential component called feedback-related negativity (FRN) or reward positivity (RewP). Recent studies have shown that FRN/RewP is markedly reduced when the action-feedback interval is long (e.g. 6000 ms), indicating that an increase in the action-feedback interval can undermine the evaluative processing of the valence of action feedback. The aim of the present study was to investigate whether or not such undermined evaluative processing of delayed action feedback could be restored by improving the accuracy of the prediction in terms of the timing of action feedback. With a typical gambling task in which the participant chose one of two cards and received an action feedback indicating monetary gain or loss, the present study showed that FRN/RewP was significantly elicited even when the action-feedback interval was 6000 ms, when an auditory stimulus sequence was additionally presented during the action-feedback interval as a temporal cue. This result suggests that the undermined evaluative processing of delayed action feedback can be restored by increasing the accuracy of the prediction on the timing of the action feedback.

Age-Related Declines in Early Sensory Memory: Identification of Rapid Auditory and Visual Stimulus Sequences.

Age-related temporal-processing declines of rapidly presented sequences may involve contributions of sensory memory. This study investigated recall for rapidly presented auditory (vowel) and visual (letter) sequences presented at six different stimulus onset asynchronies (SOA) that spanned threshold SOAs for sequence identification. Younger, middle-aged, and older adults participated in all tasks. Results were investigated at both equivalent performance levels (i.e., SOA threshold) and at identical physical stimulus values (i.e., SOAs). For four-item sequences, results demonstrated best performance for the first and last items in the auditory sequences, but only the first item for visual sequences. For two-item sequences, adults identified the second vowel or letter significantly better than the first. Overall, when temporal-order performance was equated for each individual by testing at SOA thresholds, recall accuracy for each position across the age groups was highly similar. These results suggest that modality-specific processing declines of older adults primarily determine temporal-order performance for rapid sequences. However, there is some evidence for a second amodal processing decline in older adults related to early sensory memory for final items in a sequence. This selective deficit was observed particularly for longer sequence lengths and was not accounted for by temporal masking.

No rapid audiovisual recalibration in adults on the autism spectrum.

Autism spectrum disorders (ASD) are characterized by difficulties in social cognition, but are also associated with atypicalities in sensory and perceptual processing. Several groups have reported that autistic individuals show reduced integration of socially relevant audiovisual signals, which may contribute to the higher-order social and cognitive difficulties observed in autism. Here we use a newly devised technique to study instantaneous adaptation to audiovisual asynchrony in autism. Autistic and typical participants were presented with sequences of brief visual and auditory stimuli, varying in asynchrony over a wide range, from 512 ms auditory-lead to 512 ms auditory-lag, and judged whether they seemed to be synchronous. Typical adults showed strong adaptation effects, with trials proceeded by an auditory-lead needing more auditory-lead to seem simultaneous, and vice versa. However, autistic observers showed little or no adaptation, although their simultaneity curves were as narrow as the typical adults. This result supports recent Bayesian models that predict reduced adaptation effects in autism. As rapid audiovisual recalibration may be fundamental for the optimisation of speech comprehension, recalibration problems could render language processing more difficult in autistic individuals, hindering social communication.

Motion-induced disturbance of auditory-motor synchronization and its modulation by transcranial direct current stimulation.

The timing of personal movement with respect to external events has previously been investigated using a synchronized finger-tapping task with a sequence of auditory or visual stimuli. While visuomotor synchronization is more accurate with moving stimuli than with stationary stimuli, it remains unclear whether the same principle holds true in the auditory domain. Although the right inferior-superior parietal lobe (IPL/SPL), a center of auditory motion processing, is expected to be involved in auditory-motor synchronization with moving sounds, its functional relevance has not yet been investigated. The aim of the present study was thus to clarify whether horizontal auditory motion affects the accuracy of finger-tapping synchronized with sounds, as well as whether the application of transcranial direct current stimulation (tDCS) to the right IPL/SPL affects this. Nineteen healthy right-handed participants performed a task in which tapping was synchronized with both stationary sounds and sounds that created apparent horizontal motion. This task was performed before and during anodal, cathodal and sham tDCS application to the right IPL/SPL in separate sessions. The time difference between the onset of the sounds and tapping was larger with apparently moving sounds than with stationary sounds. Cathodal tDCS decreased this difference, anodal tDCS increased the variance of the difference and sham stimulation had no effect. These results supported the hypothesis that auditory motion disturbs efficient auditory-motor synchronization and that the right IPL/SPL plays an important role in tapping in synchrony with moving sounds via auditory motion processing.

The 3-Second Rule in Hereditary Pure Cerebellar Ataxia: A Synchronized Tapping Study

The ‘3-second rule’ has been proposed based on miscellaneous observations that a time period of around 3 seconds constitutes the fundamental unit of time related to the neuro-cognitive machinery in normal humans. The aim of paper was to investigate the temporal processing in patients with spinocerebellar ataxia type 6 (SCA6) and SCA31, pure cerebellar types of spinocerebellar degeneration, using a synchronized tapping task. Seventeen SCA patients (11 SCA6, 6 SCA31) and 17 normal age-matched volunteers participated. The task required subjects to tap a keyboard in synchrony with sequences of auditory stimuli presented at fixed interstimulus intervals (ISIs) between 200 and 4800 ms. In this task, the subjects required non-motor components to estimate the time of forthcoming tone in addition to motor components to tap. Normal subjects synchronized their taps to the presented tones at shorter ISIs, whereas as the ISI became longer, the normal subjects displayed greater latency between the tone and the tapping (transition zone). After the transition zone, normal subjects pressed the button delayed relative to the tone. On the other hand, SCA patients could not synchronize their tapping with the tone even at shorter ISIs, although they pressed the button delayed relative to the tone earlier than normal subjects did. The earliest time of delayed tapping appearance after the transition zone was 4800 ms in normal subjects but 1800 ms in SCA patients. The span of temporal integration in SCA patients is shortened compared to that in normal subjects. This could represent non-motor cerebellar dysfunction in SCA patients.

A theory of attentional modulations of the supratemporal generation of the auditory mismatch negativity (MMN).

.Itisthe taking possession by the mind in clearand vivid form, of one out of what seemseveral simultaneously possible objects ortrains of thought. Focalization, concentra-tion, of consciousness are of its essence.It implies withdrawal from some thingsin order to deal effectively with others,and is a condition which has a real oppo-site in the confused, dazed, scatterbrainedstate which in French is called distrac-tion, and Zerstreutheit in German,” thuswrote James (1890, pp. 403–404). In thefield of auditory cognitive neuroscience, itwould seem everyone knows what atten-tionisnot—thegenerationoftheauditorymismatch negativity (MMN).However, Erlbeck et al.’s (2014) resultsshow that attention affects the audi-tory MMN component that can con-tribute to the auditory Long-LatencyResponses (ALLRs) of the auditory Event-Related Potential (ERP); an ERP com-ponent generated by the electrochem-ical responses of populations of neu-ronal elements firing synchronously inresponse to unexpected acoustical changesin sequences of auditory stimuli. Erlbecket al.’s findings contest the received wis-dom that this auditory MMN componentis elicited in a task-independent uncon-sciouspre-attentivemanner.Erlbecketal.’sinterpretation is best-placed with respectto the history of auditory cognitive neuro-science, to which this commentary turns,leading into an appraisal of the implica-tions of Erlbeck et al. study for under-standing MMN and auditory attention.Hillyard et al. (1973) originally identi-fied the “early selective attention effect” asascalpnegativityoccurringduringthefirstmajor negative deflection of the ALLRsof the auditory ERP: the N1. An oddballsequence—comprised of a repeated stan-dard

Auditory temporal preparation induced by rhythmic cues during concurrent auditory working memory tasks.

The present study investigated whether participants can develop temporal preparation driven by auditory isochronous rhythms when concurrently performing an auditory working memory (WM) task. In Experiment 1, participants had to respond to an auditory target presented after a regular or an irregular sequence of auditory stimuli while concurrently performing a Sternberg-type WM task. Results showed that participants responded faster after regular compared with irregular rhythms and that this effect was not affected by WM load; however, the lack of a significant main effect of WM load made it difficult to draw any conclusion regarding the influence of the dual-task manipulation in Experiment 1. In order to enhance dual-task interference, Experiment 2 combined the auditory rhythm procedure with an auditory N-Back task, which required WM updating (monitoring and coding of the information) and was presumably more demanding than the mere rehearsal of the WM task used in Experiment 1. Results now clearly showed dual-task interference effects (slower reaction times [RTs] in the high- vs. the low-load condition). However, such interference did not affect temporal preparation induced by rhythms, with faster RTs after regular than after irregular sequences in the high-load and low-load conditions. These results revealed that secondary tasks demanding memory updating, relative to tasks just demanding rehearsal, produced larger interference effects on overall RTs in the auditory rhythm task. Nevertheless, rhythm regularity exerted a strong temporal preparation effect that survived the interference of the WM task even when both tasks competed for processing resources within the auditory modality.

Preattentive dysfunction in patients with bipolar disorder as revealed by the pitch-mismatch negativity: a magnetoencephalography (MEG) study.

Mismatch negativity (MMN) and its magnetic counterpart (MMNm) are thought to reflect an automatic process that detects a difference between an incoming stimulus and the sensory memory trace of preceding stimuli. In patients with schizophrenia, an attenuation of the MMN/MMNm amplitude has been repeatedly reported. Heschl's gyrus (HG) is one of the major generators of MMN and the functional alteration of HG has been reported in patients with bipolar disorder. The present study investigated the pitch-MMNm in patients with bipolar disorder using whole-head 306-ch magnetoencephalography (MEG). Twenty-two patients and 22 healthy controls participated in this study. Subjects were presented with two types of auditory stimulus sequences. One consisted of 1,000 Hz standards (probability = 90%) and 1,200 Hz deviants (probability = 10%), and the other consisted of 1,000 Hz standards (90%) and 1,200 Hz deviants (10%). These two tasks were each performed twice. Event-related brain responses to standard tones were subtracted from responses to deviant tones. Patients with bipolar disorder showed a significant bilateral reduction in magnetic global field power (mGFP) amplitudes (p = 0.02) and dipole moments of the MMNm (p = 0.04) compared with healthy controls. Patients with admission experience showed significantly reduced mGFP amplitudes of MMNm compared with patients without admission experience (p = 0.004). Additionally, patients with more severe manic symptoms had smaller mGFP amplitudes of MMNm (ρ = -0.50, p = 0.05). The results of this study suggest that patients with bipolar disorder may exhibit preattentive auditory dysfunction indexed by reduced pitch-MMNm responses. Pitch-MMNm could be a potential trait marker reflecting the global severity of bipolar disorder.

Isolating the Neural Mechanisms of Interference during Continuous Multisensory Dual-task Performance

The need to engage in multiple tasks simultaneously is often encountered in everyday experience, but coordinating between two or more tasks can lead to impaired performance. Typical investigations of multitasking impairments have focused on the performance of two tasks presented in close temporal proximity on discrete trials; however, such paradigms do not match well with the continuous performance situations more typically encountered outside the laboratory. As a result, the stages of information processing that are affected during multisensory continuous dual tasks and how these changes in processing relate to behavior remain unclear. To address these issues, participants were presented simultaneous rapid visual and auditory stimulus sequences under three conditions: attend visual only, attend auditory only, and dual attention (attend both visual and auditory). Performance, measured in terms of response time and perceptual sensitivity (d'), revealed dual-task impairments only in the auditory task. Neural activity, measured by the ERP technique, revealed that both early stage sensory processing and later cognitive processing of the auditory task were affected by dual-task performance, but similar stages of processing of the visual task were not. Critically, individual differences in neural activity at both early and late stages of information processing accurately rank-ordered individuals based on the observed difference in behavioral performance between the single and dual attention conditions. These results reveal relationships between behavioral performance and the neural correlates of both early and late stage information processing that provide key insights into the complex interplay between the brain and behavior when multiple tasks are performed continuously.

Neuroscientific evidence for defensive avoidance of fear appeals.

Previous studies indicate that people respond defensively to threatening health information, especially when the information challenges self-relevant goals. The authors investigated whether reduced acceptance of self-relevant health risk information is already visible in early attention allocation processes. In two experimental studies, participants were watching high- and low-threat health commercials, and at the same time had to pay attention to specific odd auditory stimuli in a sequence of frequent auditory stimuli (odd ball paradigm). The amount of attention allocation was measured by recording event-related brain potentials (i.e., P300 ERPs) and reaction times. Smokers showed larger P300 amplitudes in response to the auditory targets while watching high-threat instead of low-threat anti-smoking commercials. In contrast, non-smokers showed smaller P300 amplitudes during watching high as opposed to low threat anti-smoking commercials. In conclusion, the findings provide further neuroscientific support for the hypothesis that threatening health information causes more avoidance responses among those for whom the health threat is self-relevant.

The role of auditory transient and deviance processing in distraction of task performance: a combined behavioral and event-related brain potential study

Distraction of goal-oriented performance by a sudden change in the auditory environment is an everyday life experience. Different types of changes can be distracting, including a sudden onset of a transient sound and a slight deviation of otherwise regular auditory background stimulation. With regard to deviance detection, it is assumed that slight changes in a continuous sequence of auditory stimuli are detected by a predictive coding mechanisms and it has been demonstrated that this mechanism is capable of distracting ongoing task performance. In contrast, it is open whether transient detection—which does not rely on predictive coding mechanisms—can trigger behavioral distraction, too. In the present study, the effect of rare auditory changes on visual task performance is tested in an auditory-visual cross-modal distraction paradigm. The rare changes are either embedded within a continuous standard stimulation (triggering deviance detection) or are presented within an otherwise silent situation (triggering transient detection). In the event-related brain potentials, deviants elicited the mismatch negativity (MMN) while transients elicited an enhanced N1 component, mirroring pre-attentive change detection in both conditions but on the basis of different neuro-cognitive processes. These sensory components are followed by attention related ERP components including the P3a and the reorienting negativity (RON). This demonstrates that both types of changes trigger switches of attention. Finally, distraction of task performance is observable, too, but the impact of deviants is higher compared to transients. These findings suggest different routes of distraction allowing for the automatic processing of a wide range of potentially relevant changes in the environment as a pre-requisite for adaptive behavior.

Does Rapid Auditory Stimulation Accelerate an Internal Pacemaker? Don’t Bet on It

Several interesting studies in the literature have demonstrated that a temporal interval coinciding with or following a rapid sequence of auditory stimuli is subjectively lengthened relative to a baseline interval without such rapid auditory stimulation (RAS). It has also been found that an interval preceding RAS is subjectively shortened. These effects have been attributed to acceleration of an internal pacemaker by RAS. The present study used musically trained participants in two experiments, similar to some reported in the literature. In Experiment 1, rapid chromatic scales preceded, followed, or intervened between two empty intervals that had to be compared. In Experiment 2, a series of comparison intervals, each preceded by a series of rapidly repeated tones, had to be compared to a memorized standard interval. Neither experiment yielded any effects of RAS relative to a control condition without RAS. These negative results raise questions about the conditions under which RAS affects interval judgment, and whether pacemaker acceleration is the correct explanation for these effects when they do occur.

Action enhances auditory but not visual temporal sensitivity

People naturally dance to music, and research has shown that rhythmic auditory stimuli facilitate production of precisely timed body movements. If motor mechanisms are closely linked to auditory temporal processing, just as auditory temporal processing facilitates movement production, producing action might reciprocally enhance auditory temporal sensitivity. We tested this novel hypothesis with a standard temporal-bisection paradigm, in which the slope of the temporal-bisection function provides a measure of temporal sensitivity. The bisection slope for auditory time perception was steeper when participants initiated each auditory stimulus sequence via a keypress than when they passively heard each sequence, demonstrating that initiating action enhances auditory temporal sensitivity. This enhancement is specific to the auditory modality, because voluntarily initiating each sequence did not enhance visual temporal sensitivity. A control experiment ruled out the possibility that tactile sensation associated with a keypress increased auditory temporal sensitivity. Taken together, these results demonstrate a unique reciprocal relationship between auditory time perception and motor mechanisms. As auditory perception facilitates precisely timed movements, generating action enhances auditory temporal sensitivity.

Neural generators of the auditory evoked potential components P3a and P3b.

The aim of the present study was to define the scalp topography of the two subcomponents of the P3 component of the auditory evoked potential elicited in a three-stimulus oddball paradigm and to identify their cortical generators using the standardized low resolution electromagnetic tomography (sLORETA). Subjects were presented with a random sequence of auditory stimuli and instructed to respond to an infrequently occurring target stimulus inserted into a sequence of frequent standard and rare non-target stimuli. Results show that the magnitude of the frontal P3a is determined by the relative physical difference among stimuli, as it was larger for the stimulus more deviant from the standard. Major neural generators of the P3a were localized within frontal cortex and anterior cingulate gyrus. In contrast to this, the P3b, showing maximal amplitude at parietal locations, was larger for stimuli demanding a response than for the rare non-target. Major sources of the P3b included the superior parietal lobule and the posterior part of the cingulate gyrus. Our findings are in line with the hypothesis that P3a is related to alerting activity during the initial allocation of attention, while P3b is related to activation of a posterior network when the neuronal model of perceived stimulation is compared with the attentional trace.

The effects of tailored and threatening nutrition information on message attention. Evidence from an event-related potential study

Recent evidence indicates that attention is higher for individually tailored as compared to non-tailored health communications. The present study examined whether the predicted increased attention for the tailored as opposed to general nutrition education messages is moderated by presenting high vs. low threat information about the negative consequences of an unhealthy diet. In a mixed subject experimental design, undergraduate students (N=34) were reading tailored and non-tailored nutrition education messages with either high or low threat information about the negative consequences of an unhealthy diet. At the same time, they had to pay attention to specific odd auditory stimuli in a sequence of frequent auditory stimuli (oddball paradigm). The amount of attention allocation was measured by recording event-related potentials (ERPs; i.e., N100, MMN, P300) and reaction times. Result revealed main effects of tailoring and threat, indicating that more attention resources were allocated to tailored vs. non-tailored messages and to low threat vs. high threat messages. The findings confirm that tailoring is an effective means to draw attention to health messages, whereas threat information seems to result in a loss in message attention.

A multisensory investigation of the functional significance of the “pain matrix”

Functional neuroimaging studies in humans have shown that nociceptive stimuli elicit activity in a wide network of cortical areas commonly labeled as the “pain matrix” and thought to be preferentially involved in the perception of pain. Despite the fact that this “pain matrix” has been used extensively to build models of where and how nociception is processed in the human brain, convincing experimental evidence demonstrating that this network is specifically related to nociception is lacking. The aim of the present study was to determine whether there is at least a subset of the “pain matrix” that responds uniquely to nociceptive somatosensory stimulation. In a first experiment, we compared the fMRI brain responses elicited by a random sequence of brief nociceptive somatosensory, non-nociceptive somatosensory, auditory and visual stimuli, all presented within a similar attentional context. We found that the fMRI responses triggered by nociceptive stimuli can be largely explained by a combination of (1) multimodal neural activities (i.e., activities elicited by all stimuli regardless of sensory modality) and (2) somatosensory-specific but not nociceptive-specific neural activities (i.e., activities elicited by both nociceptive and non-nociceptive somatosensory stimuli). The magnitude of multimodal activities correlated significantly with the perceived saliency of the stimulus. In a second experiment, we compared these multimodal activities to the fMRI responses elicited by auditory stimuli presented using an oddball paradigm. We found that the spatial distribution of the responses elicited by novel non-target and novel target auditory stimuli resembled closely that of the multimodal responses identified in the first experiment. Taken together, these findings suggest that the largest part of the fMRI responses elicited by phasic nociceptive stimuli reflects non nociceptive-specific cognitive processes.

The time-course of auditory and visual distraction effects in a new crossmodal paradigm

Vision often dominates audition when attentive processes are involved (e.g., the ventriloquist effect), yet little is known about the relative potential of the two modalities to initiate a “break through of the unattended”. The present study was designed to systematically compare the capacity of task-irrelevant auditory and visual events to withdraw attention from the other modality. Sequences of auditory and visual stimuli were presented with different amounts of temporal offset to determine the presence, strength, and time-course of attentional orienting and reorienting as well as their impact on task-related processing. One of the streams was task-relevant, while crossmodal distraction caused by unexpected events in the other stream was measured by impairments of behavioral task performance and by the N2, P3a, and reorienting negativity (RON) components of the event-related potential (ERP). Unexpected events in the visual modality proved to be somewhat more salient than those in the auditory modality, yet crossmodal interference caused by auditory stimuli was more pronounced. The visual modality was relatively constrained in terms of a critical time-range within which distraction effects could be elicited, while the impact of auditory stimuli on task-related processing extended over a longer time-range. These results are discussed in terms of functional differences between the auditory and visual modalities. Further applications of the new crossmodal protocol are deemed promising in view of the considerable size of the obtained distraction effects.

1. Sequential analysis of the ERP 300 in young adults

Introduction: The discrimination from a random stimulus in one sequence of rhythmic auditory stimuli generates a potential in which the fundamental wave is P300 (or ERP300). These cognitive potentials have a high inter-subject variability, but less is known on intra-subject variability over time. Objective: The aim of this study is to determine whether the amplitude and the latency of scalp ERP300 maintain their general characteristics over time in young adults. Methodology: Oddball paradigm was used to generate ERP300 elicited after a set of 50 stimuli. Potentials were recorded on Fz, Cz, C3 and C4 in 30 healthy subjects. During each session subjects were submitted to two consecutive trials. The same protocol was repeated on the same day at an interval of 6 h (from early morning to afternoon) and again one week later. Results: No statistically significant differences were found in terms of week period (p > 0.05) or morning and afternoon analysis (p > 0.05). Topographically, the scalp distribution of P300 varied significantly in terms of amplitude and latency; Cz amplitude was significantly higher (p < 0.05) and latency was significantly lower (p < 0.05) when compared to the remaining scalp locations. Significant differences in amplitude were found between the first and the second trial in each one of the recording sessions (lower in the second trial, p < 0.05). Comments: The results suggested an individual stability on ERP 300 latency when studied at different times of the same day, which remained stable a week later. However, lower amplitude in the second trial is difficult to explain.

Language lateralization using MEG beta frequency desynchronization during auditory oddball stimulation with one-syllable words

Some patients with epilepsy have difficulty performing complex language tasks due to the long duration of the disease and cognitive side effects of antiepileptic drugs. Therefore, a simple passive paradigm would be useful for determining the language dominance lateralization in epilepsy patients. The goal of this study was to develop an efficient and non-invasive analysis method for determining language dominance in epilepsy patients. To this end, magnetoencephalography was performed while an auditory stimulus sequence comprised of two one-syllable spoken words was presented to 17 subjects in an oddball paradigm without subject response. The time-frequency difference between deviant and standard sounds was then analyzed in the source space using a spatial filtering method that was based on minimum-norm estimation. The laterality index was estimated in language-related regions of interest (ROI). The results were compared to the traditional lateralization method using the Wada test. Beta band oscillation activity decreased during deviant stimulation, and the lateralization of the decrease was in good agreement with the Wada test, in the posterior part of the inferior frontal gyrus in 94% of the subjects and in the posterior part of the superior temporal gyrus in 71% of the subjects. In conclusion, the ROI-based time-frequency difference between deviant and standard sounds can be used to assess language lateralization in accordance with the Wada test.