Sequence Of Attachment Research Articles

Childhood Unpredictability is Associated With Religious Coping Through Attachment to God and Divine Forgiveness

Because a majority of the world’s population is religious and believes in some higher power, it is important to understand what may facilitate or hinder religious psychological processes that give rise to well-being. The current work therefore threads together behavioral ecological, attachment, and forgiveness theoretical perspectives to assess candidate correlates of divine forgiveness and religious coping. Study 1 ( N = 441) showed, via a single path model, that childhood unpredictability was positively associated with avoidant attachment to God, which was in turn negatively associated with divine forgiveness, such that avoidant attachment to God fully mediated the association between childhood unpredictability and divine forgiveness. Study 2 ( N = 417) extended the investigation to religious coping, again analyzing data via a single path model. Childhood unpredictability was associated with positive (but not negative) coping through a mediation sequence of insecure attachment (avoidant and anxious) and, in turn, divine forgiveness. For avoidance, unpredictability was positively associated with avoidant attachment, which was negatively associated with divine forgiveness, which was positively associated with positive religious coping. For anxious attachment, unpredictability was positively associated with anxious attachment, which was positively associated with divine forgiveness, which was positively associated with positive religious coping. Notably, avoidant attachment was also curvilinearly associated with anxious attachment and coping, such that people high and low (vs. average) in avoidance are high in anxious attachment as well as positive and negative religious coping. Thus, the work provides insights important to both the study of early childhood environments and the study of religious psychological processes.

Trauma and social pathways to psychosis: Examining the role of attachment, social rank and dissociation in a clinical sample.

The trauma and social pathways model of psychosis proposes interactions between trauma, attachment, social rank and dissociation in pathways to psychosis, though this model has yet to be empirically investigated. The primary aim of this study was to examine the overall predictive value of the trauma and social pathways model using regression analysis. A secondary aim was to delineate hypothesized pathways between trauma and positive symptoms of psychosis using serial mediation analysis. This was a cross-sectional study of people attending mental health services for a psychosis-related diagnosis (N = 71). Measures of trauma, positive symptoms of psychosis, attachment, social comparison and dissociation were completed. A model of recurrent trauma, insecure attachment, social rank and dissociation predicted 23.2% of the variance in positive symptoms of psychosis. Recurrent trauma, attachment and dissociation contributed significantly to the model, while social rank did not. Further, serial mediation analysis indicated that the sequence of disorganized attachment and dissociation fully mediated the relationship between recurrent trauma and positive symptoms. Results provide preliminary support for the trauma and social pathways model of psychosis, specifically as it relates to recurrent trauma, insecure attachment and dissociation. Results did not support the social rank component of this model, however. These findings provide clear targets for the development of next-wave psychological interventions that focus on trauma-related variables in psychosis. Future studies should replicate these findings with a larger clinical sample, and consider a measure of shame to further elucidate social processes in pathways to psychosis.

MRET treated water as a tool to mitigate mRNA jab side effects: A review

The viral RNA (ribonucleic acid) was found in virtually every organ in the body, which means the spike proteins as well. There are antibodies (like the “vaccine” is supposed to create) but they’re irrelevant because, based on a study from Japan, we now know that the spike S1 protein is what does the damage. That means the spike proteins created by the mRNA will be in every organ as well, and we now know it is the spike proteins that do the damage. Another significant mRNA jab side effect was found by Israeli researchers. They discovered a link between Pfizer’s COVID-19 vaccine and a rare blood disease called thrombotic thrombocytopenic purpura (TTP). Any formation of proteins depends on Van der Waals interactions. The Van der Waals forces depend in its turn on dielectric property of the protein molecules and water since all biochemistry needs water environment. It is practically impossible to change dielectric property/electrical charge of the proteins, but it is quite easy to modify dielectric property of water. There are research data indicating relation between dielectric constant of the human body tissue (TDC) and temperature: 35° C - 74.9 F/m (TDC) and 40° C - 73.2 F/m (TDC). It shows that normal homeostasis of the human body is allowed at certain range of electrodynamic van der Walls interactions following the small range of tissue dielectric property about 75 - 73 F/m. It allows us to suggest that dielectric property of human body tissue is very important physiological parameter. Considering mentioned above ideas, it allows us to hypothesize that most of biochemical proteins building mechanisms in a healthy human body require certain physiological range “window” of van der Waals interactions and hydrogen bonding between proteins molecules and water - salt medium. In theory, this physiological “window” of van der Waals weak electromagnetic forces may be significantly different from the range of electrodynamic van der Waals interactions required for life sustains formations of DNA/RNA proteins of viruses. Thus, modification of water - based medium electrodynamic parameters of the human tissues that are favorable for the homeostasis of the body (in the range of physiological “window”) can lead to significant change of van der Walls interactions and hydrogen bonding that may result in the inhibition and interruption of proper formation of spike proteins chains. Such scenario obviously disables coronavirus life sequence of attachment and fusion with human cell membranes. We suggest such agent which can interrupt pathogenic microorganism’s life sequence is MRET (Molecular Resonance Effect technology) water with anomalous electrodynamic characteristics. MRET water can be consumed on the regular basis by human subjects to prevent infections of pathogenic microorganisms.

Mechanism of MRET Treated Water Inhibition Effect on Morphology of Pathogenic Microorganisms: A Review

We found the significant protective properties of MRET water confirmed by substantial decrease of Staphylococcus aureus CFU (colony forming units) in homogenate of kidneys of mice on MRET water compared to control group of mice on regular water following the intra-peritoneal staphylococcal infection. Another study relates to a detailed observation of the effect of MRET activated water with the modified molecular structure, physical and electrodynamic characteristics on metabolic activity and growth of conditionally pathogenic microbiological culture Escherichia coli K-12 (E.coli) and on metabolic activity of microbial associations (similar to microbial associations in the intestine). Activated (MRET) water produced significant changes of E.coli morphology: different shape, color and size of cells, disturbance of process of cellular division. We also observed positive results of MRET water complimentary treatment for HIV patients during the clinical trial. There are recent research data which allows pointing to a similar mode of action for the two viral proteins, suggesting that anti-viral strategy that targets the viral-induced membrane fusion step can be adapted from HIV-1 to SARS-CoV. The stability of virus spike protein structure is based on the overall interactions of van der Walls weak electrodynamic forces and hydrogen bonding. It is obvious, that all biochemical formations of proteins require presence of water molecules in biological systems. The overall observed research data suggest that modification of water - based medium electrodynamic parameters of the human tissues (that are favourable for the homeostasis of the body in the range of physiological “window”) can lead to significant change of van der Walls interactions and hydrogen bonding that result in the inhibition and interruption of virus spike proteins formation. Such scenario obviously disables virus life sequence of attachment and fusion with human cell membranes. The same type of mechanism can be adopted for the inhibition of other lines of pathogenic microorganisms. The studies conducted at AltheaDx Technology, USA confirm that MRET activated water based medium did not affect the morphology of normal PBMC cells on genetic level; it affected the morphology of normal PBMC cells in a positive way increasing their viability. MRET Activated Water is produced with the help of patented in the USA Molecular Resonance Effect Technology (MRET, US Patent # 6022479). We suggest that MRET water consumption by human subjects can lead to physiologically favorable modification of dielectric permittivity and hydrogen bonding of water –based medium in the human body tissues. It can provide the initial human body natural defence against intervention and spread of pathogenic microorganisms

Attachment-based mechanisms underlying capture and release of pollen grains.

Successful insect pollination can be achieved by a sequence of numerous attachment and detachment events at various biological surfaces. However, the quantitative measurements of pollen adhesion on biological surfaces have been poorly studied so far. We performed atomic force microscopy adhesion measurements of pollen on two most important floral parts for Asteraceae in a course of pollination: the stigma and style of Hypochaeris radicata plant. The results indicated distinct adhesive properties of them-the pollen adhesion on stigmatic surfaces drastically increased over prolonged contact time, while the pollen adhesion increase on stylar surfaces was rather restrained. Based on the observation with cryo-scanning electron microscopy, we explained the experimental results by the presence of morphological features in form of flexible stigmatic papillae that may play a crucial role in enhancing both capillary attraction and van der Waals forces. The distinct adhesive properties seemingly originate from the unique adhesive tasks that each of the floral parts requires to achieve successful pollination. The insights into the adhesive interaction between pollen and the floral parts, obtained in the present study, may lead to better understanding of pollination mechanisms, which are strongly related to our food production. Additionally, the novel pollen adhesive mechanisms learned from the stigma of the studied Asteraceae plant can inspire biomimetic designs of spontaneous gripping systems.

The benthic sea-silk-thread displacement of a sessile bivalve, Pinctada imbricata radiata (Leach, 1819) in the Arabian-Persian Gulf.

A number of molluscs within the Class Bivalvia are defined by their ability to secrete fine silk like threads known as byssus which are used to anchor themselves to solid substrates. With relatively few exceptions the majority of these species remain in a sedentary state throughout their life attached via their byssal threads. However, observations of adult Pinctada imbricata radiata pearl oysters made during this study revealed this species’ ability to implement active movement. Byssal threads were secreted in a sequence of attachment and detachment phases, which resulted in the active displacement of the oyster. The oyster was observed, in the laboratory over a 9 day period, travelling a distance of 28cm in a horizontal path. After horizontal displacement, a vertical climbing phase was observed until the oyster reached the water surface at which point the byssus was discarded and the animal dropped, drifting in accordance with water current intensity. It is possible that these adaptations of byssal use are a result of environmentally induced evolutionary change within P. i. radiata.

Initial Inoculation Concentration Does Not Affect Final Bacterial Colonization of In vitro Vascular Conduits.

Despite improved peri-operative care, prosthetic graft infections continue to cause substantial morbidity and mortality. Contemporary graft infection models have tested a conduit's infectability using varying concentrations without standardization. Using a static assay in vitro model, we sought to evaluate the impact of inoculation concentration on vascular conduit attachment. The 2-hour and 24-hour attachment of Staphylococcus aureus TCH1516 and Pseudomonas aeruginosa PA01-UW were determined on polytetrafluoroethylene (PTFE), Dacron®, nitinol, cobalt chromium, and Viabahn® (W.L. Gore and Associates, Newark, DE) endoprotheses. Individually and in combination, concentrations at 104, 105, 106, 107, and 108 were tested on 2-mm sections of each graft. After each time interval, the prosthetics were rinsed to remove non-attached bacteria, sonicated to release the attached bacteria, spiral plated, and then analyzed for the attached concentration. After two hours, the higher initial inoculation concentration translated into a higher attachment percentage, but the mean attachment percentage was only 14.8% in the 108 group. Pseudomonas aeruginosa had the greatest mean attachment across all material and concentration groups. The sequence of attachment on the conduits followed a constant order: Dacron, PTFE, cobalt, nitinol, and Viabahn with no difference between Dacron and PTFE. Although there were still differences at the 24-hour mark, the median attachment at each concentration was greater than the highest initial concentration (108). Initial attachment percentage is poor consistently regardless of inoculation concentration, however, Staphylococcus aureus and Pseudomonas aeruginosa are still able to achieve full attachment after 24 hours. A concentration of less than 107 should be used in vascular graft infection models to ensure adequate bacterial attachment.

A Novel Biosynthetic Pathway of Archaetidyl-myo-inositol via Archaetidyl-myo-inositol Phosphate from CDP-archaeol and d-Glucose 6-Phosphate in Methanoarchaeon Methanothermobacter thermautotrophicus Cells

Ether-type inositol phospholipids are ubiquitously distributed in Archaea membranes. The present paper describes a novel biosynthetic pathway of the archaeal inositol phospholipid. To study the biosynthesis of archaetidylinositol in vitro, we prepared two possible substrates: CDP-archaeol, which was chemically synthesized, and myo-[(14)C]inositol 1-phosphate, which was enzymatically prepared from [(14)C]glucose 6-phosphate with the inositol 1-phosphate (IP) synthase of this organism. The complete structure of the IP synthase reaction product was determined to be 1l-myo-inositol 1-phosphate, based on gas liquid chromatography with a chiral column. When the two substrates were incubated with the Methanothermobacter thermautotrophicus membrane fraction, archaetidylinositol phosphate (AIP) was formed along with a small amount of archaetidylinositol (AI). The two products were identified by fast atom bombardment-mass spectrometry and chemical analyses. AI was formed from AIP by incubation with the membrane fraction, but AIP was not formed from AI. This finding indicates that archaeal AI was synthesized from CDP-archaeol and d-glucose 6-phosphate via myo-inositol 1-phosphate and AIP. Although the relevant enzymes were not isolated, three enzymes are implied: IP synthase, AIP synthase, and AIP phosphatase. AIP synthase was homologous to yeast phosphatidylinositol synthase, and we confirmed AIP synthase activity by cloning the encoding gene (MTH1691) and expressing it in Escherichia coli. AIP synthase is a newly found member of the enzyme superfamily CDP-alcohol phosphatidyltransferase, which includes a wide range of enzymes that attach polar head groups to ester- and ether-type phospholipids of bacterial and archaeal origin. This is the first report of the biosynthesis of ether-type inositol phospholipids in Archaea.

Nanocrystalline Diamond as a Coating for Joint Implants: Cytotoxicity and Biocompatibility Assessment

Nanocrystalline diamond (NCD) coatings combine a very low surface roughness with the outstanding diamond properties, such as superlative hardness, low self‐friction coefficient, high wear and corrosion resistance, and biotolerance, which are ideal features for applications in medicine (knee and hip replacement) and surgical tools. The present work presents a comprehensive study of the cytotoxicity and biocompatibility of NCD films grown by hot‐filament chemical vapour deposition (HFCVD) technique, aiming such future applications. Cytotoxicity was evaluated in vitro by seeding human gingival fibroblasts on the NCD surface for 14 days, while specific biocompatibility was assessed on samples seeded with human bone marrow‐derived osteoblasts during 21 days. The NCD coatings proved to be noncytotoxic in the preliminary human gingival fibroblast cell cultures, as denoted by a notable sequence of cell attachment, spreading, and proliferation events. In the specific biocompatibility assay envisaging bone tissue applications, NCD coatings induced human osteoblast proliferation and the stimulation of differentiation markers, compared to standard polystyrene tissue culture plates.

Designing porous scaffolds for tissue engineering

Biomaterials are either modified natural or synthetic materials, with an appropriate response in the host tissue, which find application in a wide spectrum of implants and prostheses used in reconstructive medicine. The subsequent integration and longevity of the implanted device depends on the effectiveness of the associated biological repair. Hence, there has been considerable interest in the development of novel, second generation, biomaterials, which are favourably bioactive in terms of promoting the desired cellular response in vivo. Such biomaterials in a porous form can also act as cellular scaffolds and allow in vitro, as well as in vivo incorporation of the appropriate tissue cells, with potential control of the sequence of cell attachment, proliferation and the production of extra-cellular matrix. Such generic tissue engineering depends critically on the porous architecture of the biomaterial scaffold so as to allow both the cellular ingress and vascularization required to create a living tissue. The particular requirements of tissue-engineering scaffolds with respect to macro- and micro-porosity, as well as chemistry, are reviewed.

Comments on the mechanism of attachment in species of the monogenean genus Gyrodactylus

Abstract. In species of the monogenean helminth Gyrodactylus, the opisthaptor is the main organ of attachment to the host. The opisthaptor comprises two large centrally positioned hooks or hamuli and sixteen peripherally distributed marginal hooks. This paper describes the functional morphology and the mechanism and sequence of attachment in this species. Information on the attachment process was gathered from observations of live gyrodactylids, from transmission electron microscopy, from scanning electron microscopy of skeletal elements, and by histochemical and X‐ray elemental analysis of hook chemical composition. The marginal hooks provide the principal force of attachment whilst the hamuli are not actively employed in the process of attachment. Instead, the hamuli provide a system preventing accidental dislodgement and assist the action of the marginal hooks. Attachment is achieved by the alternating action of two systems of muscles attached respectively to the hamuli and to the marginal hooks. Relaxation or contraction of the muscles connected to the hamuli manoeuvres the hamuli over the extremities of the accessory ventral bar and allows them to pivot around their longitudinal axis, effectively raising or lowering the opisthaptoral dome. Under reduced opisthaptoral tension, the independent gaffing activity of the marginal hooks ensures a secure attachment to the host's epidermis. Repositioning of the hamuli then raises the opisthaptoral dome to tension the peripheral marginal hooks. The sequence of attachment is complete when all the muscles associated with the hooks are in a state of relaxation but are held securely and under tension by the surrounding, stretched, opisthaptoral dome.

Radical-based asymmetric synthesis: an iterative approach to 1, 3, 5, ... (2n + 1) polyols.

[formula: see text] A conceptually novel approach to 1, 3, 5, ... (2n + 1) polyols based on iterative stereo-controlled homologation of chiral hydroxyalkyl radicals is reported. Starting from alpha-keto ester precursors, the general sequence of (1) ketone reduction, (2) auxiliary attachment, (3) saponification, (4) Barton esterification, and (5) radical addition provided the two-carbon homologue in 70-80% overall yield. The simplicity and generality of this iterative strategy for 1, 3, 5, ... (2n + 1) polyol synthesis suggests an attractive alternative for the preparation of molecules containing this structural motif.

Sequence analysis of the cDNA encoding the precursor of equinatoxin V, a newly discovered hemolysin from the sea anemone Actinia equina

A cDNA encoding the 214-amino-acid (aa) precursor of equinatoxin V (EqtV) has been isolated from an Actinia equina cDNA library. The sequence of the mature toxin is preceded, as that of EqtII, by a signal peptide of 19 aa and a hydrophilic propeptide of 16 aa ending with a pair of basic residues. This is similar to the precursors of calitoxins from another sea anemone Calliactis parasitica and to those of some antimicrobial peptides of the magainin and dermaseptin families from vertebrates. The deduced aa sequence of the potential cell attachment Arg-Gly-Asp motif-containing EqtV shows 82% identity to that of EqtII.

Protocol for FAB MS/MS characterization of terpene disaccharides of wine

Application of fast atom bombardment tandem mass spectrometry (FAB MS/MS) in positive and negative ion modes to mixtures of glycosides obtained from Riesling wine after DCCC, HPLC, and flash chromatography provided the molecular mass of the glycosides, the sequence of sugar attachment, and information about the aglycons

Analysis of glycosylphosphatidylinositol membrane anchors by electrospray ionization-mass spectrometry and collision induced dissociation.

The multi-component nature of glycosylphosphatidylinositol membrane anchors makes the analysis of their structure complex. Nuclear magnetic resonance spectroscopy of delipidated glycosylphosphatidylinositol-peptide fractions can supply considerable information but requires relatively large quantities of material. High-sensitivity sequencing techniques are available for the oligosaccharide portions of glycosylphosphatidylinositol anchors, but there is no simple and generally applicable technique to complement this information. In this paper we describe the application of electrospray ionization-mass spectrometry and collision induced dissociation to study intact glycosylphosphatidylinositol-peptides from a Trypanosoma brucei variant surface glycoprotein. Collision of the [M + 4H]4+ pseudomolecular ions of two glycosylphosphatidylinositol-peptide glycoforms produced easily interpretable daughter ion spectra, from which detailed information on the lipid moiety, carbohydrate sequence and site of peptide attachment could be obtained. All of the collision induced dissociation cleavage events occurred in the glycosylphosphatidylinositol portion of the glycosylphosphatidylinositol-peptide. This technique supplies complementary data to the high-sensitivity oligosaccharide sequencing procedures and should greatly assist glycosylphosphatidylinositol anchor structure-function studies, particularly when sample quantities are limiting.

Isolation and structural characterization of the pectic polysaccharide rhamnogalacturonan II from walls of suspension-cultured rice cells

Rhamnogalacturonan II (RG-II) is a structurally complex pectic polysaccharide that had previously been isolated from the primary cell-walls of a dicot (sycamore, Acer pseudoplatanus) and a gymnosperm (Douglas fir, Pseudotsuga menziesii). Herein are described the isolation and structural characterization of RG-II from the primary cell-walls of a suspension-cultured monocot (rice, Oryza sativa). The glycosyl-linkage composition of rice RG-II was similar to that of sycamore and Douglas fir RG-II. Partial hydrolysis with acid was used to release, from rice RG-II, two disaccharides, a heptasaccharide, and an octasaccharide; all of these oligosaccharides had previously been shown to be components of sycamore RG-II. The rice RG-II disaccharides characterized were α-rhamnosyl-(1→5)-3-deoxy- d- manno-2-octulosonic acid (KDO) and β-arabinosyl-(1→5)-3-deoxy- d- lyxo-2-heptulosaric acid (DHA). The rice RG-II heptasaccharide contained, inter alia, aceric acid (3- C-carboxy-5-deoxy- l-xylose) and 2- O-methylfucose, and was shown to have the same residue sequence and same points of attachment of glycosyl linkages (except for that to aceric acid, which was not determined) as its sycamore counterpart. Finally, an octasaccharide containing, inter alia, terminal 2- O-methyl-xylose, 2-linked glucuronic acid, and 3,4-linked fucose was isolated after partial hydrolysis of rice RG-II with acid. The same octasaccharide had been postulated to be a component of sycamore RG-II; conclusive evidence of its existence as a component of rice RG-II was obtained by using fast-atom-bombardment mass spectrometric analysis. The results indicated that RG-II from the primary cell-walls of a monocot is indistinguishable from the previously characterized RG-II isolated from a dicot.

Specific phases of root hair attachment in the Rhizobium trifolii-clover symbiosis.

The time course and orientation of attachment of Rhizobium trifolii 0403 to white clover root hairs was examined in slide cultures by light and electron microscopy. Inocula were grown for 5 days on defined BIII agar medium and represented the large subpopulation of fully encapsulated single cells which uniformly bind the clover lectin trifoliin A. When 10(7) cells or more were added per seedling, bacteria attached within minutes, forming randomly oriented clumps at the root hair tips. Several hours later, single cells attached polarly to the sides of the root hair. This sequence of attachment to clover root hairs was selective for R. trifolii at inoculum sizes of 10(7) to 4 X 10(8) per seedling, specifically inhibited if 2-deoxy-D-glucose, a hapten for trifoliin A, was present in the inoculum, and not observed when 4 X 10(8) cells were added to alfalfa seedling roots or to large clover root cell wall fragments which lacked trifoliin A but still had trifoliin A receptors. Once attached, R. trifolii 0403 became progressively less detachable with 2-deoxy-D-glucose. At smaller inoculum sizes (10(5) to 10(6) cells per seedling), there was no immediate clumping of R. trifolii at clover root hair tips, although polar binding of bacteria along the root hair surface was observed after 4 h. The interface between polarly attached bacteria and the root hair cell wall was shown to contain trifoliin A by immunofluorescence microscopy. Also, this interface was shown by transmission electron microscopy to contain electron-dense granules of host origin. Scanning electron microscopy revealed an accumulation of extracellular microfibrils associated with the lateral and polar surfaces of the attached bacteria, detectable after 12 h of incubation with seedling roots. At this same time, there was a significant reduction in the effectiveness of 2-deoxy-D-glucose in dislodging bacteria already attached to root hairs and an increase in firm attachment of bacteria to the root hair surface, which withstood the hydrodynamic shear forces of high-speed vortexing. These results are interpreted as a sequence of phases in attachment, beginning with specific reversible interactions between bacterial and plant surfaces (phase I attachment), followed by production of extracellular microfibrils which firmly anchor the bacterium to the root hair (phase 2 adhesion). Thus, attachment of R. trifolii to clover root hairs is a specific process requiring more than just the inherent adhesiveness of the bacteria to the plant cell wall.(ABSTRACT TRUNCATED AT 400 WORDS)

Secondary lambda attachment site in the threonine operon attenuator of Escherichia coli.

We have determined the nucleotide sequence of a secondary gamma attachment (att) site which overlaps the Escherichia coli threonine (thr) operon attenuator. The secondary att site shows uninterrupted homology (8 out of 15) with the 15 base-pair "common core" sequence found in gamma and at the primary bacterial attachment site. These 8 base paired also overlap the thr operon attenuator. Comparison of the secondary att site with the flanking prophage sites shows that the crossover site for gamma integration lies within the -7 to 0 region of the core. Sequences on both sides of the core show no obvious homology with analogous sequences of the gamma or primary bacterial att sites. The core sequences of the left prophage att site is completely homologous to the wild-type core and also shows the same 8-base pair overlap with the thr operon attenuator. The position of the thr operon attenuator, relative to the left prophage att site, indicates that ribonucleic acid transcripts, initiated at a gamma promoter, are terminated efficiently at the thr attenuator. It is also possible that this prophage att site is able to undergo int dependent site-specific recombination which with another nearby secondary att site. Evidence is also presented which suggests that a base or sequence to the left of position -6 in the core is necessary fo excisive recombination.

Design and Performance of Systems for Cleaning Product-Contact Surfaces of Food Equipment: A Review

This paper reviews the routine and periodic systems used to clean food equipment. The manner in which soil accumulates on surfaces, and factors affecting that accumulation are discussed. The mechanisms by which microbial development on the equipment surface is controlled by the system is also discussed. The contamination sequence of deposition, attachment, depletion, growth and contamination by the organism is presented in detail. Changes in surface microbial numbers over time are reviewed for system components and complete systems. The relationship between the amount of soil on the surface and microbial survival is examined. The ability of the routine and periodic systems to control soil accumulation and microbial development is discussed. Factors affecting design of a cleaning system are also presented. These factors are used to design a cleaning system, using a pipeline milking machine as an example.

Orientation of the band 3 polypeptide from human erythrocyte membranes. Identification of NH2-terminal sequence and site of carbohydrate attachment.

The NH2-terminal sequence and carbohydrate attachment site of the 95,000-dalton transmembrane polypeptide (Band 3) from human erythrocyte membranes have been studied. The blocked NH2-terminal sequence is Ac-Met-Glu-Glu; the presence of this sequence in specific fragments of the polypeptide confirms that the end of the polypeptide which is inside the cell is the NH2-terminal. The carbohydrate associated with the Band 3 polypeptide appears to be attached at a single site in the COOH-terminal third of the molecule, to a region with composition Asx1Ser2; this confirms that part of the polypeptide toward the COOH-terminal is outside the cell. The carbohydrate structure appears to be extremely heterogeneous both in size and composition, which probably causes the Band 3 polypeptide to migrate as a diffuse band on dodecyl sulfate gel electrophoresis.