Separate Sequences Research Articles

Spelling Bees

In this note, we consider a form of competition in which two contestants face separate sequences of independent trials. The contestant with the longer sequence of successful trials wins. However, since the trials are independent, there may be a form of paradox whereby the loser is able to pass all of the trials in the winner’s sequence, whereas the winner could not have overcome two or more trials in the loser’s sequence. We refer to this as the spelling bee paradox and explore its likelihood in simple settings. JEL Classification Codes: C46, D74, Z29.

Integrated analysis of scRNA-seq and bulk RNA-seq identifies FBXO2 as a candidate biomarker associated with chemoresistance in HGSOC

BackgroundHigh-grade serous ovarian carcinoma (HGSOC) is the most prevalent and aggressive histological subtype of epithelial ovarian cancer. Around 80% of individuals will experience a recurrence within five years because of resistance to chemotherapy, despite initially responding well to platinum-based treatment. Biomarkers associated with chemoresistance are desperately needed in clinical practice. MethodsWe jointly analyzed the transcriptomic profiles of single-cell and bulk datasets of HGSOC to identify cell types associated with chemoresistance. Copy number variation (CNV) inference was performed to identify malignant cells. We subsequently analyzed the expression of candidate biomarkers and their relationship with patients' prognosis. The enrichment analysis and potential biological function of candidate biomarkers were explored. Then, we validated the candidate biomarker using in vitro experiments. ResultsWe identified 8871 malignant epithelial cells in a single-cell RNA sequencing dataset, of which 861 cells were associated with chemoresistance. Among these malignant epithelial cells, FBXO2 (F-box protein 2) is highly expressed in cells related to chemoresistance. Moreover, FBXO2 expression was found to be higher in epithelial cells from chemoresistance samples compared to those from chemosensitivity samples in a separate single-cell RNA sequencing dataset. Patients exhibiting elevated levels of FBXO2 experienced poorer outcomes in terms of both overall survival (OS) and progression-free survival (PFS). FBXO2 could impact chemoresistance by influencing the PI3K-Akt signaling pathway, focal adhesion, and ECM-receptor interactions and regulating tumorigenesis. The 50% maximum inhibitory concentration (IC50) of cisplatin decreased in A2780 and SKOV3 ovarian carcinoma cell lines with silenced FBXO2 during an in vitro experiment. ConclusionsWe determined that FBXO2 is a potential biomarker linked to chemoresistance in HGSOC by combining single-cell RNA-seq and bulk RNA-seq dataset. Our results suggest that FBXO2 could serve as a valuable prognostic marker and potential target for drug development in HGSOC.

How to make a bridal bouquet: sensory knowing in action

This study explored the planning and making of a bridal bouquet in classroom interaction between a teacher and a student in a Swedish upper-secondary adult floristry education school. The purpose was to empirically reveal floristry disciplinary aesthetics. Aesthetics can be said to involve the exploration of sensory perception in general, entailing a focus on tacit sensory knowing. Methodologically, this study drew on the principles of ethnomethodology and (multimodal) conversation analysis to investigate video-recorded empirical data. The analysis included three separate sequences of interaction after the student requested the teacher’s attention. In the sequences, the student repeatedly provided answers to her own known-answer questions, and it remained her privilege to define what should be done and why as a consequence of the teacher’s authoritative guiding and gentle support. The results include examples of floristry disciplinary aesthetics in action when making a bridal bouquet, such as airiness and the role of outer shape. Moreover, in situ aesthetic judgement as part of sensory knowing is shown to be ample in the form of embodied actions, such as showing with the hands and communicating with facial expressions.

A rapid method for simultaneously determining activity of 89Sr and 90Sr in a non-equilibrated 90Sr/90Y system without Cerenkov counting.

A method for determining activity of 89Sr and 90Sr in a sample where 90Sr and 90Y are not in equilibrium is presented. The method consists of an experimental design and equations for accurately calculating activity of 89Sr and 90Sr based on 90Y particle counts and the total counts of particles emitted from 89Sr, 90Sr, and 90Y in a sample. The equations are derived based on chemical separation sequences, particle counting sequences, and the Bateman equation. The presented method allows simultaneously obtaining the activity of 89Sr and 90Sr in a non-equilibrated 90Sr/90Y system without the need of Cerenkov counting.

Molecular Dynamics Simulation of the Adsorption and Diffusion of C8 Aromatic Isomers in MIL-47(V).

The separation of C8 aromatic isomers (oX: o-xylene, pX: p-xylene, mX: m-xylene, and EB: ethylbenzene) remains an enormous challenge in industrial production due to their similar molecular structures and physical properties. Porous materials with suitable pore structures and selective recognition sites to discriminate the slight structural differences of isomers are imminently needed. In this paper, MIL-47(V) with a three-dimensional (3D) grid structure of 10.5 × 10.5 Å2 and a one-dimensional (1D) diamond channel was selected as the adsorbent. However, the mechanism of the adsorption and separation of C8 aromatic isomers in porous materials still needs to be understood. Given the importance of C8 aromatic isomers' confinement in MIL-47(V) for adsorption and diffusion applications, it is important to understand C8 aromatic isomers' behavior in MIL-47(V). Here, we demonstrated from a simulation perspective that metal-organic frameworks MIL-47(V) with one-dimensional (1D) diamond channels can identify C8 aromatic isomers. Molecular dynamics (MD) simulations have shown that organic ligands with guest response sites of MIL-47(V) can effectively distinguish between C8 aromatic isomers by adaptation to the shape of a specific isomer. MIL-47(V) has high adsorption and an excellent separation sequence between C8 aromatic isomers: oX > pX ≈ mX > EB. Significant differences exist in π-π superposition interactions between C8 aromatic isomers and between C8 aromatic isomers and the skeletons. This phenomenon is mainly caused by the unique pore structure and guest response characteristics of MIL-47(V). This work is identified as a supplementary instruction to experimental research and is expected to provide profound insights into research on developing C8 aromatic isomers' adsorption and separation and theoretical support.

Stratigraphic architecture of the synrift interval in the northern Pletmos Basin, offshore South Africa

A revised analysis of the stratigraphy and the order of deposition in the Synrift interval is conducted for the northern Pletmos Basin, offshore South Africa. The study area, spanning 14,330 km2, includes the Superior and Plettenberg grabens. By employing new techniques in seismic stratigraphy, a more accurate identification of sedimentary layers in the basin was achieved, taking into account their seismic characteristics, internal structure, and termination points. The findings primarily focus on two distinct phases of geological activity known as Synrift I and Synrift II. The Kimmeridgian interval of Synrift I has not been extensively explored, and is believed to exhibit petroleum potential, as indicated by the presence of oil and gas shows and the distinctive seismic features of structural closures. Within the Synrift interval, ten separate sequences were identified, each displaying different seismic characteristics and environments of deposition. By utilizing these predicted depositional environments, numerous potential drilling sites were identified within the Synrift interval. Furthermore, it was determined that these sites were supplied with hydrocarbons from three hypothesized source intervals. Consequently, this research enhances our understanding of the potential for hydrocarbon reserves in the northern Pletmos Basin.

Occurrence and evolutionary conservation analysis of α-helical cationic amphiphilic segments in the human proteome.

The existence of encrypted fragments with antimicrobial activity in human proteins has been thoroughly demonstrated in the literature. Recently, algorithms for the large-scale identification of these segments in whole proteomes were developed, and the pervasiveness of this phenomenon was stated. These algorithms typically mine encrypted cationic and amphiphilic segments of proteins, which, when synthesized as individual polypeptide sequences, exert antimicrobial activity by membrane disruption. In the present report, the human reference proteome was submitted to the software kamal for the uncovering of protein segments that correspond to putative intragenic antimicrobial peptides (IAPs). The assessment of the identity of these segments, frequency, functional classes of parent proteins, structural relevance, and evolutionary conservation of amino acid residues within their corresponding proteins was conducted in silico. Additionally, the antimicrobial and anticancer activity of six selected synthetic peptides was evaluated. Our results indicate that cationic and amphiphilic segments can be found in 2% of all human proteins, but are more common in transmembrane and peripheral membrane proteins. These segments are surface-exposed basic patches whose amino acid residues present similar conservation scores to other residues with similar solvent accessibility. Moreover, the antimicrobial and anticancer activity of the synthetic putative IAP sequences was irrespective to whether these are associated to membranes in the cellular setting. Our study discusses these findings in light of the current understanding of encrypted peptide sequences, offering some insights into the relevance of these segments to the organism in the context of their harboring proteins or as separate polypeptide sequences.

A neural code for time and space in the human brain

Time and space are primary dimensions of human experience. Separate lines of investigation have identified neural correlates of time and space, yet little is known about how these representations converge during self-guided experience. Here, 10 subjects with intracranially implanted microelectrodes play a timed, virtual navigation game featuring object search and retrieval tasks separated by fixed delays. Time cells and place cells activate in parallel during timed navigation intervals, whereas a separate time cell sequence spans inter-task delays. The prevalence, firing rates, and behavioral coding strengths of time cells and place cells are indistinguishable-yet time cells selectively remap between search and retrieval tasks, while place cell responses remain stable. Thus, the brain can represent time and space as overlapping but dissociable dimensions. Time cells and place cells may constitute a biological basis for the cognitive map of spatiotemporal context onto which memories are written.

High-resolution lithostratigraphy and reconnaissance sedimentology of Changotaung structure, Chittagong Tripura fold belt, Bengal Basin, Bangladesh

Unlike other structures in the vicinity of the Chittagong Tripura Fold Belt, the Changotaung anticline is one of Bangladesh's least explored structures. An attempt has been made for the first time to understand and document sedimentary deposits, environments, structure, and tectonic activity based on the high-resolution outcrop and reconnaissance study with the knowledge of broad-brush geology. We found that Changotaung is a symmetrical box-folded structure with an extensive western flank where the amount of dip varies between 11° and 45°. The exposed Cenozoic succession was categorized into three separate sedimentary sequences and correlated with the conventional stratigraphic unit. A first-order simple Markovian approach was presented for the exposed litho-section in an effort to illustrate vertical facies variations in the Upper Surma group. We quantified that heterolithic bed mostly overlies both trough cross-bedding ({P}_{ij}U = 0.706) and parallel laminated bed ({P}_{ij}U=0.955) according to the facies transition probability matrix. According to the results of the stationary distribution, there is a 40% chance of coming across heterolithic beds within the Upper Surma group during any given event that is completely random whereas trough cross-bedding, parallel laminated bed, cumulative sandstone facies, and cumulative shale facies shows around 10.8%, 15.2%, 20.6%, 13.4% probability. We hypothesized, based on the interpretive facies analysis, that the Chittagong Tripura fold belt region's Upper Surma Group underwent three interrelated depositional settings (wave-dominated shallow marine, tide-dominated shallow marine, and fluvio-deltaic distributary).

Simultaneous perfusion, diffusion, T2 *, and T1 mapping with MR fingerprinting.

Quantitative mapping of brain perfusion, diffusion, T2 *, and T1 has important applications in cerebrovascular diseases. At present, these sequences are performed separately. This study aims to develop a novel MRI technique to simultaneously estimate these parameters. This sequence to measure perfusion, diffusion, T2 *, and T1 mapping with magnetic resonance fingerprinting (MRF) was based on a previously reported MRF-arterial spin labeling (ASL) sequence, but the acquisition module was modified to include different TEs and presence/absence of bipolar diffusion-weighting gradients. We compared parameters derived from the proposed method to those derived from reference methods (i.e., separate sequences of MRF-ASL, conventional spin-echo DWI, and T2 * mapping). Test-retest repeatability and initial clinical application in two patients with stroke were evaluated. The scan time of our proposed method was 24% shorter than the sum of the reference methods. Parametric maps obtained from the proposed method revealed excellent image quality. Their quantitative values were strongly correlated with those from reference methods and were generally in agreement with values reported in the literature. Repeatability assessment revealed that ADC, T2 *, T1 , and B1 + estimation was highly reliable, with voxelwise coefficient of variation (CoV) <5%. The CoV for arterial transit time and cerebral blood flow was 16% ± 3% and 25% ± 9%, respectively. The results from the two patients with stroke demonstrated that parametric maps derived from the proposed method can detect both ischemic and hemorrhagic stroke. The proposed method is a promising technique for multi-parametric mapping and has potential use in patients with stroke.

Design and multi-objective optimization of reactive pressure-swing distillation process for separating tetrahydrofuran-methanol-water

The low-energy and low-cost process development and design has always been a hot topic and challenge in separating azeotropic systems in the world. In this paper, a novel, energy-saving reactive pressure-swing distillation (RPSD) was, for the first time, designed for separating the ternary mixture of tetrahydrofuran (THF)-methanol–water. In the reactive distillation column, the process of reacting water with ethylene oxide to produce ethylene glycol for the first time was used to separate THF-methanol–water mixture, then the residual mixtures were separated by two pressure-swing distillation columns. Two feasible RPSD separation processes were investigated in terms of thermodynamic modeling and T-xy phase diagram analysis to achieve optimal separation sequence. The two designed separation processes were optimized by a multi-objective genetic algorithm and heat integration to maximize economic benefit. Results demonstrated that, compared to the conventional pressure-swing distillation process, the total annual cost of the designed two heat-integrated processes based on the RPSD-B process were greatly reduced, i.e., 45.9% and 52.2%, respectively. This paper has important guiding significance for the separation and purification of multicomponent azeotropes.

Exon 44 skipping in Duchenne muscular dystrophy: NS-089/NCNP-02, a dual-targeting antisense oligonucleotide

Exon-skipping therapy mediated by antisense oligonucleotides is expected to provide a therapeutic option for Duchenne muscular dystrophy. Antisense oligonucleotides for exon skipping reported so far target a single continuous sequence in or around the target exon. In the present study, we investigated antisense oligonucleotides for exon 44 skipping (applicable to approximately 6% of all Duchenne muscular dystrophy patients) to improve activity by using a novel antisense oligonucleotide design incorporating two connected sequences. Phosphorodiamidate morpholino oligomers targeting two separate sequences in exon 44 were created to target two splicing regulators in exon 44 simultaneously, and their exon 44 skipping was measured. NS-089/NCNP-02 showed the highest skipping activity among the oligomers. NS-089/NCNP-02 also induced exon 44 skipping and dystrophin protein expression in cells from a Duchenne muscular dystrophy patient to whom exon 44 skipping is applicable. We also assessed the invivo activity of NS-089/NCNP-02 by intravenous administration to cynomolgus monkeys. NS-089/NCNP-02 induced exon 44 skipping in skeletal and cardiac muscle of cynomolgus monkeys. In conclusion, NS-089/NCNP-02, an antisense oligonucleotide with a novel connected-sequence design, showed highly efficient exon skipping both invitro and invivo.

Applicability of supercritical fluid chromatography for oligonucleotide analysis: A proof-of-concept study

We evaluated the suitability of supercritical fluid chromatography (SFC) for oligonucleotide analysis using 4-mer oligonucleotides with various phosphorothioate (PS) contents as model compounds. Column screening showed that the diol-modified column was able to separate sequences with different PS contents. Optimization of the column body and additives allowed us to analyze polar oligonucleotides using SFC. Various sequences were also analyzed using the optimized method. A good peak shape was obtained when the guanine plus cytosine content of the analyte was two or less in the 4-mer oligonucleotides. Furthermore, we found that the retention times of the selected sequences were positively correlated with polar surface areas, indicating that oligonucleotides interact with polar stationary phases. In contrast, more hydrophobic full PS sequences were retained more strongly in the diol column than the full phosphodiester (PO) sequences. This suggests that the diol column has unique selectivity for PO and PS linkages. These results indicate that SFC is potentially applicable to oligonucleotide analysis with a separation mechanism that is different from that of ion-pair reversed-phase liquid chromatography.

Structural Characteristics of Mitochondrial Genomes of Eight Treehoppers (Hemiptera: Membracidae: Centrotinae) and Their Phylogenetic Implications.

Complete mitochondrial genomes were newly sequenced for eight species of the treehopper subfamily Centrotinae (Hemiptera: Membracidae), four of which represent genera for which mitogenomes were not previously available. The new mitogenomes are generally similar in overall structure, gene order, base composition, and nucleotide content to those of previously sequenced species of the subfamily. Phylogenetic analyses were conducted using both maximum likelihood and Bayesian inference methods based on three separate nucleotide sequence datasets in which RNA gene sequences and/or third codon positions were either included or excluded from the concatenated protein-coding gene alignments. The results are consistent with previous phylogenies based on morphology and partial nuclear genome data, except for the lack of support for the monophyly of Leptocentrini. These results show that mitogenome sequences are informative of both ancient and recent divergence patterns within Centrotinae.

Duality for asymptotic invariants of graded families

The starting point of this paper is a duality for sequences of natural numbers which, under mild hypotheses, interchanges subadditive and superadditive sequences and inverts their asymptotic growth constants.We are motivated to explore this sequence duality since it arises naturally in at least two important algebraic-geometric contexts. The first context is Macaulay-Matlis duality, where the sequence of initial degrees of the family of symbolic powers of a radical ideal is dual to the sequence of Castelnuovo-Mumford regularity values of a quotient by ideals generated by powers of linear forms. This philosophy is drawn from an influential paper of Emsalem and Iarrobino. We generalize this duality to differentially closed graded filtrations of ideals.In a different direction, we establish a duality between the sequence of Castelnuovo-Mumford regularity values of the symbolic powers of certain ideals and a geometrically inspired sequence we term the jet separation sequence. We show that this duality underpins the reciprocity between two important geometric invariants: the multipoint Seshadri constant and the asymptotic regularity of a set of points in projective space.

A framework for threat intelligence extraction and fusion

Cyber-attacks, with various emerging attack techniques, are becoming increasingly sophisticated and difficult to deal with, posing great threats to companies and every individual. Therefore, analyzing attack incidents and tracing the attack groups behind them becomes extremely important. Threat intelligence provides a new technical solution for attack traceability by constructing Cybersecurity Knowledge Graph (CKG). In this paper, we propose a framework for threat intelligence extraction and fusion, which is able to extract, correlate and unify cybersecurity entity-relation triples from structured and unstructured data. However, the existing entity and relation extraction for cybersecurity concepts uses the traditional pipeline model that suffers from error propagation and ignores the connection between the two subtasks. To solve the above problem, we propose a joint entity and relation extraction model for cybersecurity concepts. We model the joint extraction problem as a multiple sequence labeling problem, generating separate label sequences for different relations, which contain information about the involved entities and the subject and object of that relation. Experimental results on Open Source Intelligence (OSINT) data show that the F1 value of the joint model is 81.37%, which is better than the previous pipeline model. For the knowledge fusion, we propose an improved Levenshtein distance to correlate the same entities extracted from different data sources to construct a preliminary CKG, which is demonstrated in the Experiments section.

Sex-specific associations in multiparametric3 T MRI measurements in adult livers.

MRI relaxometry mapping and proton density fat fraction (PDFF) have been proposed for the evaluation of hepatic fibrosis. However, sex-specific relationships of age and body fat with these MRI parameters have not been studied in detail among adults without clinically manifest hepatic disease. We aimed to determine the sex-specific correlation of multiparametric MRI parameters with age and body fat and to evaluate their interplay associations. 147 study participants (84 women, mean age 48±14 years, range 19-85 years) were prospectively enrolled. 3 T MRI including T1, T2 and T1ρ mapping and PDFF and R2* map were acquired. Visceral and subcutaneous fat were measured on the fat images from Dixon water-fat separation sequence. All MRI parameters demonstrated sex difference except for T1ρ. PDFF was more related to visceral than subcutaneous fat. Per 100 ml gain of visceral or subcutaneous fat is associated with 1 or 0.4% accretion of liver fat, respectively. PDFF and R2* were higher in men (both P = 0.01) while T1 and T2 were higher in women (both P < 0.01). R2* was positively but T1 and T2 were negatively associated with age in women (all P < 0.01), while T1ρ was positively related to age in men (P < 0.05). In all studies, R2* was positively and T1ρ was negatively associated with PDFF (both P <0.0001). Visceral fat plays an essential role in the elevated liver fat. When using MRI parametric measures for liver disease evaluation, the interplay between these parameters should be considered.

Highly sensitive detection of microRNA-21 by nitrogen-doped carbon dots-based ratio fluorescent probe via nuclease-assisted rolling circle amplification strategy

Highly sensitive and selective detection of microRNA-21 (miRNA-21) in biological samples is critical for the disease diagnosis and cancer treatment. In this study, a nitrogen-doped carbon dots (N-CDs)-based ratio fluorescence sensing strategy was constructed for miRNA-21 detection with high sensitivity and excellent specificity. Bright-blue N-CDs (λex/λem = 378 nm/460 nm) were synthesized by facile one-step microwave-assisted pyrolysis method by using uric acid as the single precursor, and the absolute fluorescence quantum yield and fluorescence lifetime of N-CDs were 35.8% and 5.54 ns separately. The padlock probe hybridized with miRNA-21 firstly and then was cyclized by T4 RNA ligase 2 to form a circular template. At the present of dNTPs and phi29 DNA polymerase, the oligonucleotide sequence in miRNA-21 was prolonged to hybridize with the surplus oligonucleotide sequences in circular template, generating long and reduplicated oligonucleotide sequences containing abundant guanine nucleotides. Separate G-quadruplex sequences were generated after the addition of Nt.BbvCI nicking endonuclease, and then hemin bound with G-quadruplex sequence to construct the G-quadruplex DNAzyme. Such G-quadruplex DNAzyme catalyzed the redox reaction of o-phenylenediamine (OPD) with H2O2, finally producing the yellowish-brown 2,3-diaminophenazine (DAP) (λem = 562 nm). Due to the inner filter effect between N-CDs and DAP, the ratio fluorescence signal of DAP with N-CDs was utilized for sensitive detection of miRNA-21 with detection limit of 0.87 pM. Such approach has practical feasibility and excellent specificity for miRNA-21 analysis during highly homological miRNA family in HeLa cell lysates and human serum samples.

Conceptual process design and process optimization of triple-column pressure-swing distillation for the separation of ternary mixtures embedding two azeotropes with different feed composition via thermodynamic insights and genetic algorithm

In this article, we take the tetrahydrofuran (THF)/ethanol (ETOH)/methanol (MeOH) as an example to investigate the process design of the triple-column pressure-swing distillation (TCPSD) for the separation of Serafimov’s class 2.0-2b mixtures with different feed composition. Two basic topology structures (TSI, TSII) and two separation sequences (SSI, SSII) are investigated, then, process optimization with total annual cost (TAC) as objective function is conducted via genetic algorithms. Heat integration (HI) is also applied to TCPSD. From a new perspective, the separation of THF/ETOH/MeOH can be regarded as the azeotropic distillation for THF/ETOH with MeOH as azeotropic agents based on thermodynamic features of Serafimov’s class 2.0-2b mixtures. The feasible topology structure and separation sequence of TCPSD for Serafimov’s class 2.0-2b mixtures depend on the feed composition and operating pressure. The feed composition is divided into three regions according to the concentration of methanol in azeotropic distillation column. The two critical points are azeotropic points at allowed minimum and maximum operating pressure. Especially, when feed composition is below azeotropic point at allowed minimum operating pressure, TSI is not feasible due to less MeOH as azeotropic agents. Furthermore, TCPSD combining pure azeotropic agents (ATPSD) can be developed based on this new insight. Then, two feed compositions are used to illustrate the detailed conceptual design of three topology structures as well as corresponding two separation sequences and their economic performance. The results show that when feed composition is 0.4ETOH/0.25THF/0.35MeOH, TSI and TSII are feasible, the best economical configurations without and with heat integration is TSII-SSII and TSII-SSII-HI. When feed composition is 0.4ETOH/0.4THF/0.2MeOH, TSII and ATPSD are feasible, the best economical configurations without and with heat integration is ATPSD-SSI and ATPSD-SSI-HI. Furthermore, ATPSD-SSI and ATPSD-SSI-HI have 18.28% and 19.38% TAC reduction than TSII-SSI and TSII-SSI-HI, respectively. In summary, feasible and the best economic topology structure and separation sequence of TCPSD for Serafimov’s class 2.0-2b mixtures depend on the feed composition and operating pressure. In addition, the proposed ATPSD shows good economic performance for Serafimov’s class 2.0-2b mixtures.

Modelling, optimal solvent screening and separation of 5-hydroxymethylfurfural or furfural from catalytic conversion reactor stream in downstream purification process

An overall process evaluation-based solvent selection for the biphasic catalytic conversion of the sugars into bio-based 5-hydroxymethylfurfural (5-HMF), furfural (FURF) and for the downstream separation and purification sequence was carried out, which represents an important step in industrial operating procedure. In the chemical process screening for 5-HMF extraction, the various dissolving fluids of different molecular polarity were used, parallel batch experiments were performed, and a few were identified as potential substance solutions for either, 5-HMF or FURF. Criteria were based on the ability to form two liquid phases within an aqueous thermodynamic system, to have fast transport kinetics, and simultaneously extract the 5-HMF and/or FURF with a high partitioning coefficient. The feasibility of the constituents’ pure mixture in terms of waste material efficiency, its resource recovery and the purity of both product species were also a part of the criteria. The best solute-partitioning alternatives found, besides an organic benchmark methyl isobutyl ketone (MIBK), were benzyl alcohol, tetrahydrofuran (THF) with an addition of sodium salt and homologues, resulting from the determined efficacy analysis, modelling and optimisation of the continuous purification methodology by means of partition, distillation and thermal heat recuperation. This allows for designing a diluted carbohydrate stream integrated bio refining in the wake of pulping.