Sensory Activity Research Articles

Effects of roasting conditions on sensory attributes, polyphenolic content and DPPH radical scavenging activity of peanut (Arachis hypogaea)

Roasting is considered the commonest processing method applied to foods, it affects both phytochemicals and sensory attributes. In the present study, the effect of varying roasting parameters on sensory attributes was determined, as well as scavenging property against free radicals (DPPH) and polyphenolic content (TPC and TFC) of whole peanut kernels. Whole peanut kernels were roasted at different temperatures (130-150 °C) with various durations (5-20 min). Finding revealed that peanuts roasted at 130, 140, and 150 °C for 15, 10, and 5 min, respectively, received significantly the highest (p<0.05) sensory scores in all the parameters investigated. Therefore, they were selected for TPC, TFC, and DPPH assays. All three assays were found to increase during roasting compared to the unroasted counterpart. Peanuts that were roasted at 140 °C for 10 min, had the highest total phenolic contents of 67.26 ± 1.77 mg GAE/g while those roasted at 150 °C for 5 contained the highest TFC of 12.91 ± 0.56 mg QE/g. The highest DPPH radical scavenging activity was detected in the sample roasted at 140 °C for 10 min with an IC50 value of 417.44 μg/mL. Roasting significantly affected the bioactive contents as well as the scavenging activity of the whole peanut.

Assessing spontaneous sensory neuron activity using in vivo calcium imaging.

Heightened spontaneous activity in sensory neurons is often reported in individuals living with chronic pain. It is possible to study this activity in rodents using electrophysiology, but these experiments require great skill and can be prone to bias. Here, we have examined whether in vivo calcium imaging with GCaMP6s can be used as an alternative approach. We show that spontaneously active calcium transients can be visualised in the fourth lumbar dorsal root ganglion (L4 DRG) through in vivo imaging in a mouse model of inflammatory pain. Application of lidocaine to the nerve, between the inflamed site and the DRG, silenced spontaneous firing and revealed the true baseline level of calcium for spontaneously active neurons. We used these data to train a machine learning algorithm to predict when a neuron is spontaneously active. We show that our algorithm is accurate in 2 different models of pain: intraplantar complete Freund adjuvant and antigen-induced arthritis, with accuracies of 90.0% ±1.2 and 85.9% ±2.1, respectively, assessed against visual inspection by an experienced observer. The algorithm can also detect neuronal activity in imaging experiments generated in a different laboratory using a different microscope configuration (accuracy = 94.0% ±2.2). We conclude that in vivo calcium imaging can be used to assess spontaneous activity in sensory neurons and provide a Google Colaboratory Notebook to allow anyone easy access to our novel analysis tool, for the assessment of spontaneous neuronal activity in their own imaging setups.

MODEL TADABUR AL-QURAN BERASASKAN TAFSIRAN ULAMA TERHADAP AYAT 205 SURAH AL-A’RAF DAN AYAT 37 SURAH QAF

Quranic contemplation is one most exalted worship in Islam because its function in guiding servant to the guidance of Allah SWT. It is the purpose of the revelation of the Quran and the legislation of recitation. Failing to contemplate the Quran is the reason why the hypocrites and polytheists in the time of the Prophet Muhammad SAW were prevented from receiving guidance. Although contemplation is crucial in Islam, the development of Quranic contemplation skills does not receive proper attention in the Islamic education curriculum. In fact, the implementation of contemplation in tahfiz institutions in Malaysia is only at a moderate level. Therefore, this study aims to propose a practical model of Quranic contemplation based on the Quran itself, to explain the process of how the implementation can achieve the objectives of tazkiyah and tahalliyah in worship and generating recognizant of Allah. This study is based on a qualitative approach using document analysis as a research instrument. Research data were collected from more than 30 exegesis books involving scholar’s interpretations of verse 205 of Surah Al-A'raf and verse 37 of Surah Qaf, and analyzed using thematic analysis methods. The findings of the study show that the process of contemplation involves sensory, mental, and spiritual activities, namely, bringing the heart closer to Allah, listening effectively, thinking, reflecting, drawing lessons, and being devout. A further study is recommended for the purpose of implementing this model in the Islamic education system, especially in the tahfiz curriculum in Malaysia.

Exploring the Impact of Sensory Activities on the Development of Entrepreneurial Spirit in Children

This article explores the impact of sensory activities on fostering an entrepreneurial spirit in children. Sensory activities, involving exercises that stimulate a child’s senses, play a crucial role in cognitive development and can lay the groundwork for entrepreneurial skills. The article emphasizes how these activities enhance creativity, critical thinking, and problem-solving abilities - key components of an entrepreneurial mindset. It discusses how engaging in sensory play, arts and crafts, music, and nature walks not only provides immediate enjoyment but also develops traits like resilience, adaptability, and innovative thinking in children. By promoting a sense of curiosity and exploration, sensory activities contribute to building an entrepreneurial mindset from an early age. This approach is vital in preparing children for the complexities and demands of the future, equipping them with skills necessary for success in an ever-evolving global landscape.

A-kinase anchoring protein 79/150 coordinates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor sensitization in sensory neurons

Changes in sensory afferent activity contribute to the transition from acute to chronic pain. However, it is unlikely that a single sensory receptor is entirely responsible for persistent pain. It is more probable that extended changes to multiple receptor proteins expressed by afferent neurons support persistent pain. A-Kinase Anchoring Protein 79/150 (AKAP) is an intracellular scaffolding protein expressed in sensory neurons that spatially and temporally coordinates signaling events. Since AKAP scaffolds biochemical modifications of multiple TRP receptors linked to pain phenotypes, we probed for other ionotropic receptors that may be mediated by AKAP and contribute to persistent pain. Here, we identify a role for AKAP modulation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Receptor (AMPA-R) functionality in sensory neurons. Pharmacological manipulation of distinct AMPA-R subunits significantly reduces persistent mechanical hypersensitivity observed during hyperalgesic priming. Stimulation of both protein kinases C and A (PKC, PKA, respectively) modulate AMPA-R subunit GluR1 and GluR2 phosphorylation and surface expression in an AKAP-dependent manner in primary cultures of DRG neurons. Furthermore, AKAP knock out reduces sensitized AMPA-R responsivity in DRG neurons. Collectively, these data indicate that AKAP scaffolds AMPA-R subunit organization in DRG neurons that may contribute to the transition from acute-to-chronic pain.

Direct activation of airway sensory C-fibers by SARS-CoV-2 S1 spike protein.

Respiratory viral infection can lead to activation of sensory afferent nerves as indicated by the consequential sore throat, sneezing, coughing, and reflex secretions. In addition to causing troubling symptoms, sensory nerve activation likely accelerates viral spreading. The mechanism how viruses activate sensory nerve terminals during infection is unknown. In this study, we investigate whether coronavirus spike protein activates sensory nerves terminating in the airways. We used isolated vagally-innervated mouse trachea-lung preparation for two-photon microscopy and extracellular electrophysiological recordings. Using two-photon Ca2+ imaging, we evaluated a total number of 786 vagal bronchopulmonary nerves in six experiments. Approximately 49% of the sensory fibers were activated by S1 protein (4 μg/mL intratracheally). Extracellular nerve recording showed the S1 protein evoked action potential discharge in sensory C-fibers; of 39 airway C-fibers (one fiber per mouse), 17 were activated. Additionally, Fura-2 Ca2+ imaging was performed on neurons dissociated from vagal sensory ganglia (n = 254 from 22 mice). The result showed that 63% of neurons responded to S1 protein. SARS-CoV-2 S1 protein can lead to direct activation of sensory C-fiber nerve terminals in the bronchopulmonary tract. Direct activation of C-fibers may contribute to coronavirus symptoms, and amplify viral spreading in a population.

Catecholaminergic neuromodulation and selective attention jointly shape perceptual decision-making.

Perceptual decisions about sensory input are influenced by fluctuations in ongoing neural activity, most prominently driven by attention and neuromodulator systems. It is currently unknown if neuromodulator activity and attention differentially modulate perceptual decision-making and/or whether neuromodulatory systems in fact control attentional processes. To investigate the effects of two distinct neuromodulatory systems and spatial attention on perceptual decisions, we pharmacologically elevated cholinergic (through donepezil) and catecholaminergic (through atomoxetine) levels in humans performing a visuo-spatial attention task, while we measured electroencephalography (EEG). Both attention and catecholaminergic enhancement improved decision-making at the behavioral and algorithmic level, as reflected in increased perceptual sensitivity and the modulation of the drift rate parameter derived from drift diffusion modeling. Univariate analyses of EEG data time-locked to the attentional cue, the target stimulus, and the motor response further revealed that attention and catecholaminergic enhancement both modulated pre-stimulus cortical excitability, cue- and stimulus-evoked sensory activity, as well as parietal evidence accumulation signals. Interestingly, we observed both similar, unique, and interactive effects of attention and catecholaminergic neuromodulation on these behavioral, algorithmic, and neural markers of the decision-making process. Thereby, this study reveals an intricate relationship between attentional and catecholaminergic systems and advances our understanding about how these systems jointly shape various stages of perceptual decision-making.

Catecholaminergic neuromodulation and selective attention jointly shape perceptual decision-making

Perceptual decisions about sensory input are influenced by fluctuations in ongoing neural activity, most prominently driven by attention and neuromodulator systems. It is currently unknown if neuromodulator activity and attention differentially modulate perceptual decision-making and/or whether neuromodulatory systems in fact control attentional processes. To investigate the effects of two distinct neuromodulatory systems and spatial attention on perceptual decisions, we pharmacologically elevated cholinergic (through donepezil) and catecholaminergic (through atomoxetine) levels in humans performing a visuo-spatial attention task, while we measured electroencephalography (EEG). Both attention and catecholaminergic enhancement improved decision-making at the behavioral and algorithmic level, as reflected in increased perceptual sensitivity and the modulation of the drift rate parameter derived from drift diffusion modeling. Univariate analyses of EEG data time-locked to the attentional cue, the target stimulus, and the motor response further revealed that attention and catecholaminergic enhancement both modulated pre-stimulus cortical excitability, cue- and stimulus-evoked sensory activity, as well as parietal evidence accumulation signals. Interestingly, we observed both similar, unique, and interactive effects of attention and catecholaminergic neuromodulation on these behavioral, algorithmic, and neural markers of the decision-making process. Thereby, this study reveals an intricate relationship between attentional and catecholaminergic systems and advances our understanding about how these systems jointly shape various stages of perceptual decision-making.

The Correlation between the Structures of Bimetallic Tartrate Complexes in Solutions for Laser-induced Synthesis and Sensor Characteristics of Microbiosensors Materials

Background: Determination of diagnostically significant components of biological ma-terials using enzyme-free microscopic sensors is an urgent scientific task, which is being worked on by a significant number of scientific groups in the world. This is due to the fact that microscop-ic sensor-active tracks on inert surfaces can be obtained without preliminary manufacturing of precision templates. Methods: Laser Induced Chemical Liquid Phase Deposition (LCLD) is a laser technology that al-lows the deposition of microsized conductive tracks from aqueous solutions of transition metal compounds at the focus of a laser beam. These tracks can be formed by one or two metals at the same time. The possibility of obtaining complexes in solution in which two different metals inter-act with one common coordination sphere of the ligand is of particular interest. The structure of such complexes is still insufficiently studied. Results: The present study supplements the missing information on tartaric acid complexes, which can simultaneously coordinate two metals, for example, copper, nickel, silver, iron, and cobalt. Heterophase LCLD demonstrates high sensory activity in the electrochemical oxidation/reduction of glucose and hydrogen peroxide. Bimetallic deposits can be obtained in two ways. The first method consists of successive precipitation from a solution containing an ion of one metal, then another on top of the first. The second way is to create a solution in which two metals and one lig-and are simultaneously present. Laser deposition is carried out in one stage. In practice, the possi-bility of the second method is not always realized. Conclusion: In the present work, the basic principles of the formation of heterophase bimetallic sensor-sensitive porous material with a highly developed surface under the action of laser radia-tion have been analyzed, and new reference data have been accumulated on the structure of tar-trate complexes containing two metals.

Bayesian encoding and decoding as distinct perspectives on neural coding.

The Bayesian brain hypothesis is one of the most influential ideas in neuroscience. However, unstated differences in how Bayesian ideas are operationalized make it difficult to draw general conclusions about how Bayesian computations map onto neural circuits. Here, we identify one such unstated difference: some theories ask how neural circuits could recover information about the world from sensory neural activity (Bayesian decoding), whereas others ask how neural circuits could implement inference in an internal model (Bayesian encoding). These two approaches require profoundly different assumptions and lead to different interpretations of empirical data. We contrast them in terms of motivations, empirical support and relationship to neural data. We also use a simple model to argue that encoding and decoding models are complementary rather than competing. Appreciating the distinction between Bayesian encoding and Bayesian decoding will help to organize future work and enable stronger empirical tests about the nature of inference in the brain.

PELATIHAN KEGIATAN SENSORIAL TRAINING MONTESSORI UNTUK MAHASISWA DAN GURU PAUD

The partner educational institution, namely the Ponorogo Early Education Center (Pocenter), has not yet implemented sensory stimulation in children because educators do not yet understand more deeply how Montessori sensorial training activities work. So far the teachers only provide normal sensorial activities and do not use Montessori sensorial training. Through this training activity, it is hoped that educators at Pocenter and students as prospective educators can implement it when they graduate. This activity is the result of collaboration between four PG-PAUD study programs at Muhammadiyah universities. The activity was carried out online with six virtual meetings and involved speakers from the University of Malaysia Sarawak. The results of the pretest and posttest can be concluded that this workshop influenced the mindset of workshop participants for center teachers and prospective PAUD teacher students positively and significantly. This can be seen in the α value being smaller than 0.05, namely 0.00 and the t value being negative, read as positive because the pretest value is lower than the posttest value.

Effectiveness of a Sensory Play Activity Program with Parent Engagement for Children with Autism Spectrum Disorder: A Randomized Control Trial

Background: According to DSM-5, sensory processing problems are among diagnostic criteria in children with autism spectrum disorder (ASD). Various studies have shown that play, as the main occupation of children, can be helpful in performing sensory interventions. Solving sensory problems is one of the most common demands of families with children with ASD. Children's families play a key role in the implementation of occupational therapeutic interventions. Objectives: This randomized clinical trial investigated the effectiveness of sensory play activities performed by parents at home on the sensory patterns of children with ASD. Methods: Fifty-one children with ASD, aged 3 to 6 years, were divided into the intervention and control groups. All participants attended 16 sessions of in-person individualized sensory integration therapy (SIT) in a clinic for 8 weeks. In the intervention group, children received 8 sessions of a sensory play activity intervention performed by parents at home. Data collection instruments included the Gilliam Autism Rating Scale-2 and the sensory profile 2. Parametric (independent and paired t-test) and non-parametric (Mann-Whitney and Wilcoxon tests) were used for statistical analysis to compare between-group and within-group mean differences. Results: The results showed that although all four sensory processing patterns improved significantly in both groups (P < 0.05) (i.e., the within-group effect), children in the intervention group showed significant improvements in three sensory patterns, including sensory avoiding, sensory sensitivity, and sensory registration (P< 0.01). There was no significant difference in the mean score of the sensory seeking pattern between the 2 groups (P > 0.05). Conclusions: Sensory integration therapy improved dysfunctional sensory processing patterns in ASD children, an effect that was enhanced by the simultaneous implementation of sensory play interventions.

Hemokinin-1 induces transcriptomic alterations in pain-related signaling processes in rat primary sensory neurons independent of NK1 tachykinin receptor activation.

The tachykinin hemokinin-1 (HK-1) is involved in immunological processes, inflammation, and pain. Although the neurokinin 1 receptor (NK1R) is described as its main target, several effects are mediated by currently unidentified receptor(s). The role of HK-1 in pain is controversial, depending on the involvement of peripheral and central sensitization mechanisms in different models. We earlier showed the ability of HK-1 to activate the trigeminovascular system, but the mechanisms need to be clarified. Therefore, in this study, we investigated HK-1-induced transcriptomic alterations in cultured rat trigeminal ganglion (TRG) primary sensory neurons. HK-1 was applied for 6 or 24 h in 1 μM causing calcium-influx in these neurons, 500 nM not inducing calcium-entry was used for comparison. Next-generation sequencing was performed on the isolated RNA, and transcriptomic changes were analyzed to identify differentially expressed (DE) genes. Functional analysis was performed for gene annotation using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome databases. NK1R and Neurokinin receptor 2 (NK2R) were not detected. Neurokinin receptor 3 (NK3R) was around the detection limit, which suggests the involvement of other NKR isoforms or other receptors in HK-1-induced sensory neuronal activation. We found protease-activated receptor 1 (PAR1) and epidermal growth factor receptor (EGFR) as DE genes in calcium signaling. The transmembrane protein anthrax toxin receptor 2 (ANTXR2), a potential novel pain-related target, was upregulated. Acid-sensing ion channel 1; 3 (Asic1,3), N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) glutamate receptors decreased, myelin production and maintenance related genes (Mbp, Pmp2, Myef2, Mpz) and GNDF changed by HK-1 treatment. Our data showed time and dose-dependent effects of HK-1 in TRG cell culture. Result showed calcium signaling as altered event, however, we did not detect any of NK receptors. Presumably, the activation of TRG neurons is independent of NK receptors. ANTXR2 is a potential new target, PAR-1 has also important role in pain, however their connection to HK-1 is unknown. These findings might highlight new targets or key mediators to solve how HK-1 acts on TRG.

Neuromorphological bases of sensorimotor reactions

Sensory motor activity is a typical form of purposeful human functioning that plays a significant role in the work of power structures. Its research is widespread in all spheres of military medicine. The aim of the study is to systematize the components and principles of organization of structural and functional elements of the central nervous system providing sensorimotor reactions based on the literature data. We obtained results on the combination of the somatotopic arrangement and the hierarchical principle of organization of sensorimotor circuits, according to which three levels are distinguished - medullary, subcortical and cortical. Sensory signals are converted into explicit motor signals as early as possible, and are subsequently subjected to modulating influences from memory, attention, and other neurophysiological processes at each of the above levels. Thus, sensory and motor information processing should not be regarded as the result of isolated neural circuits, but as extensive and closely interconnected networks covering the cerebral cortex and subcortical structures, in unity with other neurophysiological phenomena, such as memory and attention. The neuromorphological basis for the organization of sensorimotor reactions should be considered when developing a set of measures for professional selection, training, pharmacological correction of combat conditions and treatment of posttraumatic syndrome in employees of power structures.

Acquisition of Arabic Students with Hybrid and Receptive Learning Models

This study aimed to analyze the ability to acquire Arabic as a second language for students of the Islamic Economics and Business Faculty at IAIN Ponorogo through hybrid and sensory learning activities. This research is qualitative with a case study type. The research population totaled 176 students, with a sample of 20 students. The sampling technique was done by non-probability sampling with the snowball sampling method because the samples were taken based on the needs of this study. The research data is in the form of pronunciation of vocabulary sounds, Arabic spelling of letters, verbs, and nouns, the ability to understand the material being listened to, and the ability to read Arabic texts for FEBI IAIN Ponorogo students in odd semesters of 2022/2023 academic year. The student reading source used as research data is the book "Al-'Arabiyyah fī Dirāsah al-Mu'āmalāt." A book taught in the course "Arabic for Economics." Observation, interviews, and documentation carry out data collection techniques. Data analysis techniques with data reduction, data presentation, concluding, and verification. The results of the study show that the acquisition of Arabic as a second language by students of the Islamic Faculty of Economics and Business at IAIN Ponorogo in attending "Arabic for Economics" courses tends to differ from one another. They are entering the pre-production language acquisition phase. That is, they have not mastered many foreign vocabulary. This is due to the educational background taken before they entered university. Another influencing factor is the environment in which students live.

Multi-sensory evaluation of urban design in creating obesity encouraging environments: The experience of town centres in Auckland, New Zealand

This article presents a multi-sensory study on pedestrian activities in the public realm of two town centers in Auckland, New Zealand. We utilize mixed-method and multi-sensory evaluation methods, combined with sensory experience and activity mapping, to explore the effects of the obesity encouraging environment of town centers on the activity concentrations of pedestrians. We further discuss the influences of urban design and land use diversity on generating obesity encouraging environments of town centers. The article aims to provide a basis to initiate discussion around the importance of understanding the multiple-sensory experience of public space beyond merely sight sense which traditionally dominates urban design discipline. Furthermore, this article demonstrates the role and quality of sensory experience in shaping obesogenic behaviors within the built environment of two town centres; with a diversity of type and quality of land uses. It also highlights that the mere provision of urban morphology or built form factors linked with walking activities is not a precursor for healthier communities. Thus, the impacts of urban design and quality uses of the built environment on the multiple senses of the users should be considered in urban design research and practices.

A competition framework for fixation-preference in strabismus.

Strabismic subjects often develop the ability to fixate on a target with either eye. Previous studies have shown that fixation-preference behavior varies systematically depending on spatial location of the target. We hypothesized that, when an eccentric target is presented, oculomotor fixation-preference in strabismus may be accounted for in a competitive decision framework wherein the brain must choose between two possible retinal errors to prepare a conjugate saccade that results in one of the eyes acquiring the eccentric target. We tested this framework by recording from visuo-motor neurons in the superior colliculus (SC) of two strabismic rhesus macaque monkeys as they performed a delayed saccade task under binocular viewing conditions. In one experiment, visual targets were presented at one of two locations corresponding to the neuronal receptive field location with respect to either the viewing or the deviated eye. Robust visual sensory responses were observed when targets were presented at either location indicating the presence of competing sensory signals for eye-choice. In a second experiment, a single visual target was placed at the neuronal receptive field location where the animal switched fixation on some trials and did not on other trials. At such target locations where either eye could acquire the target, both visual and build-up activity was greater in trials when the saccade encoded by the neuron "won." These findings provide evidence for the influence of visual suppression within SC sensory activity and support the possible utilization of a competition framework, one that has been previously described for when a binocularly aligned animal chooses from among multiple targets, to drive fixation-preference behavior in strabismus.

Dental Tissue Engineering by Neural Differentiation of Dental Stem Cells and Nano-systems: A Review

Background: Pulpitis is a pulpal inflammation. It generally occurs when there is inflammation within a tooth as a result of anything like grinding or decay. After dental inflammation, microcirculation and sensory nerve activity seem to play the most critical role in reducing inflammation. Therefore, researchers emphasize the study of dental nerve activity, especially in acute clinical problems in inflamed teeth and pulp regeneration. This review aims to investigate the possibility of using dental stem cells to regenerate dental nerves in order to repair dentin-pulp complexes for maintaining and restoring tooth structure and function, which nanosystems can help in this matter. Materials and Methods: In this paper, we review the literature regarding the theory of dental tissue engineering by neural differentiation of dental stem cells and nano-systems, and the comprehensive search on PubMed, Scopus, and Web of Science was conducted up to July 2022. Results: According to recent studies, dental soft and hard tissue healing also includes nerve fibers. A deeper understanding of how dental nerves are implicated in pulpitis may assist endodontic treatment. Stem cell-based treatments may be used to regenerate dental nerves to repair dentin-pulp complexes to maintain and restore tooth structure and function. Conclusion: The emphasis on dental nerve regeneration appears to be a critical stage in fostering spontaneous tooth regeneration as well as a sustainable tooth regeneration method. It is essential to further investigate dental tissue engineering by neural differentiation of dental stem cells.

Intrinsic and Extrinsic Risk Factors for Nosocomial Pressure Injury among Hospitalized Adults at a Tertiary Hospital in Western Kenya

The high incidence and prevalence of nosocomial pressure injuries pose a threat to safe hospitalization and additional strain on the healthcare system. Analysis of pressure injury risk factors is necessary for efficient prevention and management as part of the program to ensure that no patient experiences any preventable harm while receiving care, as per the international patient safety goals. This study evaluated the intrinsic and extrinsic risk factors for pressure injury among cohorts of hospitalized adults using a prospective approach. The Braden Scale was used to assess the participants’ level of risk and classify low (score 15 to 23) and high (score 6 to 14) cohorts prior to follow-up. Binary regression analysis was used to establish an association between the risk factors and the development of stage 2 and above pressure injuries and the odds reported. The intrinsic factors—nutrition, sex, age, and primary diagnosis—influenced the occurrence of pressure injuries. Similarly, the extrinsic factors—sensory perception, moisture, mobility and activity, presence of a medical device, friction, and shear—showed an association with the development of pressure injuries. Poor nutrition, impaired mobility, and persistent moisture, however, were statistically the most significantly associated risk factors, at OR 18.20, CI 2.18–151.69, p =.01; OR 20.93, CI 1.13–387.92, p =.04; OR 16.11, CI 2.02–128.52, p =.01, respectively, confirming a significant association between intrinsic and extrinsic risk factors and the development of hospital-acquired pressure injury. These identified risk factors can be used to tailor intervention earlier on admission and avert these preventable injuries. Standardized, timely risk assessment and proactive addressing of the identified risk factor among hospitalized patients are therefore recommended.

Overview of the Biotransformation of Limonene and α-Pinene from Wood and Citrus Residues by Microorganisms

This review provides an overview of the biotransformation of limonene and α-pinene, which are commonly found in wood residues and citrus fruit by-products, to produce high-value-added products. Essential oils derived from various plant parts contain monoterpene hydrocarbons, such as limonene and pinenes which are often considered waste due to their low sensory activity, poor water solubility, and tendency to autoxidize and polymerise. However, these terpene hydrocarbons serve as ideal starting materials for microbial transformations. Moreover, agro-industrial byproducts can be employed as nutrient and substrate sources, reducing fermentation costs, and enhancing industrial viability. Terpenes, being secondary metabolites of plants, are abundant in byproducts generated during fruit and plant processing. Microbial cells offer advantages over enzymes due to their higher stability, rapid growth rates, and genetic engineering potential. Fermentation parameters can be easily manipulated to enhance strain performance in large-scale processes. The economic advantages of biotransformation are highlighted by comparing the prices of substrates and products. For instance, R-limonene, priced at US$ 34/L, can be transformed into carveol, valued at around US$ 530/L. This review emphasises the potential of biotransformation to produce high-value products from limonene and α-pinene molecules, particularly present in wood residues and citrus fruit by-products. The utilisation of microbial transformations, along with agro-industrial byproducts, presents a promising approach to extract value from waste materials and enhance the sustainability of the antimicrobial, the fragrance and flavour industry.