Sensitive Electrochemical Biosensor Research Articles

Biomimetic Electrochemical Sensor Based on Single-Atom Nickel Laccase Nanoenzyme for Quercetin Detection.

Laccase, a member of the copper oxidase family, has been used as a green catalyst in the environmental and biochemical industries. However, laccase nanoenzymes are limited to materials with copper as the active site, and noncopper laccase nanoenzymes have been scarcely reported. In this study, inspired by the multiple copper active sites of natural laccase and the redox Cu2+/Cu+ electron transfer pathway, a novel nitrogen/nickel single-atom nanoenzyme (N/Ni SAE) with high laccase-like activity was prepared by inducing Ni and dopamine precipitation through a controllable water/ethanol interface reaction. Compared with that of laccase, the laccase activity simulated by N/Ni SAE exhibited excellent stability and reusability. The N/Ni SAE exhibited a higher efficiency toward the degradation of 2,4-dichlorophenol, hydroquinone, bisphenol A, and p-aminobenzene. In addition, a sensitive electrochemical biosensor was constructed by leveraging the laccase-like activity of N/Ni SAE; this sensor offered unique advantages in terms of catalytic activity, selectivity, stability, and repeatability. Its detection ranges for quercetin were 0.01-0.1 and 1.0-100 μM, and the detection limit was 3.4 nM. It was also successfully used for the quantitative detection of quercetin in fruit juices. Therefore, the single-atom biomimetic nanoenzymes prepared in this study promote the development of a new electrochemical strategy for the detection of various bioactive molecules and show great potential for practical applications.

Highly sensitive electrochemical biosensor for MUC1 detection based on DNA-functionalized CdTe quantum dots as signal enhancers.

In this paper, an electrochemical biosensor based on a cadmium telluride/polypyrrole (CdTe/PPy) nanocomposite was developed for the detection of MUC1 with high selectivity and sensitivity. Results indicate that the CdTe/PPy nanocomposite is modified on the surface of the glassy carbon electrode (GCE), which affords a large surface area for immobilizing cap-DNA, ensuring its high selectivity and sensitivity. Next, CdTe-linked sig-DNA (MUC1 aptamer) was introduced, allowing the MUC1 aptamer to hybridize with cap-DNA. CdTe is a signal amplification element used to generate a differential pulse voltammetry (DPV) signal. Conceivably, target MUC1 detection was based on current signal change due to concentration change in the signal amplification element CdTe. In the presence of MUC1, the MUC1 aptamer specifically binds to MUC1, resulting in the release of CdTe-sig-DNA from the electrode surface and a decrease in peak current. Under optimized experimental conditions, the electrochemical biosensor is highly selective, sensitive, stable, and reproducible for MUC1 ranging from 0.1 nM to 100 nM with a detection limit of 0.05 nM (S/N = 3). Therefore, the electrochemical biosensor has potential applications in medical disease diagnosis.

Non-ideal nernstian behavior in organic electrochemical transistors: fundamental processes and theory.

Despite the successful implementation of organic electrochemical based devices (OEDs), fundamental processes that regulate their operations and sensing capabilities, specifically those related to ion-to-electron transduction, remain unclear. Indeed, there is still a lack of fundamental models to explain the steady-state and transient characteristics of OEDs, associating fundamentals of the physical-chemistry of the pair polymer-electrolyte with the output performance of such devices. In this study, we bring new highlights to a thermodynamic-based model that qualitatively and quantitatively describes OEDs operation, with a special focus on the organic electrochemical transistor (OECT). In this context, we introduce novel interpretations for traditional drain current models, grounded in thermodynamic and electrochemical principles. The model fitting parameters are correlated to the physical and chemical properties of the polymer-electrolyte pair, and it has been shown to explain trends observed in experimental results from the literature. Moreover, our model reveals that a non-Nerstian electrochemical behavior dominates OECT operation. Also, by analyzing experimental data, we are able to generate guidelines for material design and device development, targeting highly sensitive electrochemical biosensors and devices.

Ag nanorod@PEI-Ag nanohybrid as an excellent signal label for sensitive and rapid detection of serum HER2

The accurate detection of Human epidermal growth factor receptor-2 (HER2) as a critical breast cancer biomarker can be essential for the early selection of therapeutic approaches. HER2 is a prominent component of a signaling network. Overexpression of the HER2 protein due to amplification of its gene leads to the development of an aggressive subtype of breast cancer. Patients with tumors that overexpress HER2 are eligible for treatment that significantly reduces mortality rates. Herein, we present a fast and simple method for detecting serum HER2. A new electrochemical label has been developed using charged Ag nanorod@ polyethylenimine-Ag (Ag NR@ PEI-Ag) nanohybrid. The synthesized Ag NR@PEI-Ag nanohybrid simultaneously has the electroactive property of silver and the large surface area of the PEI, which results in the enhancement of the detection signal. So, using Ag NR@PEI-Ag nanohybrid as the electrochemical label, a simple, fast, and sensitive electrochemical biosensor was designed to detect HER2. This way, after immobilizing HER2 aptamer on the Au electrode surface, HER2 or human serum was exposed to the aptamer. Then, the positively charged Ag NR@PEI-Ag nanohybrid was adsorbed onto the negatively charged aptamer-HER2 complex, and the current that was produced due to the Ag/AgCl reaction was measured as the electrochemical signal. The aptasensor shows a broad linear response from 10–12 to 10–7 g, a low detection limit (LOD) of 10 pg, and a total assay time of ~ 30 min.

Highly sensitive electrochemical peptide-based biosensor for marine biotoxin detection using a bimetallic platinum and ruthenium nanoparticle-tethered metal-organic framework modified electrode.

Saxitoxin (STX) is a highly toxic small-molecule cyanotoxin that is water-soluble, stable in acidic media, and thermostable. STX is hazardous to human health and the environment in ocean, thus it is an important to detect it at very low concentrations. Herein, we developed an electrochemical peptide-based biosensor for the trace detection of STX in different sample matrix utilizing differential pulse voltammetry (DPV) signal. We synthesized the nanocomposite of zeolitic imidazolate framework-67 (ZIF-67) decorated bimetallic platinum (Pt) and ruthenium (Ru) nanoparticles (Pt-Ru@C/ZIF-67) using impregnation method. The nanocomposite modified with screen-printed electrode (SPE) was subsequently used to detect STX in the range of 1-1,000ngmL-1, with a detection limit (LOD) of 26.7pgmL-1. The developed peptide-based biosensor is highly selective and sensitive towards STX detection, thus it represents a promising strategy for the development of novel portable bioassay for monitoring various hazardous molecules in aquatic food chains.

Highly sensitive electrochemical assay based on strand displacement polymerization-assisted CRISPR/Cas12a collateral cleavage

In this work, a novel strand displacement polymerization strategy is designed with a single hairpin-structured DNA probe acting as both of primer and template. After target miRNA-induced structural transitions, extension and displacement reactions occur at the dimer with recycled target miRNA. The generated double-stranded products further activate CRISPR/Cas12a collateral cleavage of substrate DNA at the electrode surface, which can be reflected by the silver stripping peak variations. By analyzing the reduced peak intensity, the concentration of miRNA can be determined. This method combines the strand displacement amplification and CRISPR/Cas12a’s shearing capability. Thus a highly sensitive electrochemical biosensor is established with the limit of detection as low as 31 aM. It may also inspire new ideas of electrochemical platforms integrating CRISPR/Cas system for biological studies and clinical applications.

Bio-inspired benign synthesis of Cr doped ZnO nanoparticles towards electrochemical detection of Bovine Serum Albumin

In this study, we aimed to synthesize monodisperse chromium-doped zinc oxide (Cr-ZnO) nanoparticles (NPs) through green synthesis for the construction of a highly sensitive electrochemical biosensor for BSA detection. Characterization techniques such as X-ray diffraction, FTIR spectroscopy, UV-Vis absorption, and photoluminescence studies confirmed the structural, chemical, and optical properties of the synthesized NPs. FESEM and EDS analysis revealed a spherical nodular morphology with a cauliflower-shaped structure. Nano-bio interaction studies between Cr-ZnO NPs and BSA were conducted using UV-Vis absorption and PL measurements, indicating fluorescence quenching due to complex formation. The synthesized NPs were utilized as electrode material for an electrochemical sensor, demonstrating sensitivity and selectivity in detecting BSA concentrations within the range of 1.5 nM to 48 nM. The sensor's response was linear, showing a decrease in anodic peak current response from 5.08 µA to 3.48 µA for different BSA concentrations. Remarkably, the optical interaction results mirrored the electrochemical findings, emphasizing the consistency between the two methods. The linear response of the electrochemical sensor, coupled with its optical interactions, suggests the versatility and reliability of the synthesized NPs for biosensing applications. With a simple setup requiring minimal samples, the electrochemical sensor offers an inexpensive, rapid, and sensitive screening method for BSA. These findings underscore the potential of the synthesized NPs as a promising electrochemical surface for biosensors, with implications for diagnostic and therapeutic applications, particularly in the context of cattle-related research.

A Sensitive Electrochemical DNA Biosensor for Determination of Anti-Cancer Drug Gemcitabine Based on an AuNPs/MWCNTs/Carbon Paste Electrode

GMB is a common anticancer drug that needs careful monitoring because of its variable and toxic effects. Detecting GMB in biological samples can help adjust the dosage and enhance the treatment outcomes. In this study, a novel electrochemical sensor was developed based on a carbon paste electrode modified with single stranded DNA, gold nanoparticles and multi-walled carbon nanotubes. We characterized the unmodified (bare CPE) and modified (ss-DNA/AuNPs/MWCNTs/CPE) electrodes using scanning electron microscopy (SEM), EDX analysis and cyclic voltammetry (CV) techniques. The oxidation peak current was linearly proportional to the GMB concentration in two linear ranges: 1–10 μM and 10–50 μM, with a detection limit of 0.52 μM and a limit of quantification (LOQ) of 1.75 μM. The lower linear concentration range (1–10 μM) showed a sensitivity of 5.68 A.M−1.cm−2, while the higher range (10–50 μM) showed a sensitivity of 1.34 A.M−1.cm−2. We also investigated the repeatability, applicability and reproducibility of this method for human serum samples. We tested the selectivity and practical ability of the ss-DNA/AuNPs/MWCNTs/CPE for the determination of GMB in the presence of various interfering species and human serum samples. The results indicated that the ss-DNA/AuNPs/MWCNTs/CPE was a selective, reliable and accurate electrochemical sensor for GMB.

A New Highly Sensitive Electrochemical Biosensor for Ethanol Detection Based on Gold Nanoparticles/Reduced Graphene Oxide/Polyallylamine Hydrochloride Nanocomposite.

A highly sensitive electrochemical biosensor for ethanol based on a screen-printed electrode modified with gold nanoparticles-electrochemically reduced graphene oxide-poly (allylamine hydrochloride) nanocomposite (AuNPs-ERGO-PAH) is reported in this work. Ethanol was oxidized in the presence of the oxidized form of the nicotinamide adenine dinucleotide (NAD+) in a reaction catalyzed by alcohol dehydrogenase (ADH) immobilized in sol-gel. The AuNPs-ERGO-PAH nanocomposite was used as a transducer for the electrocatalytic oxidation of the reduced form the nicotinamide adenine dinucleotide (NADH) produced in the enzyme reaction. Under the optimal conditions, the ethanol biosensor exhibits a wide dynamic range from 0.05 to 5 mM with a low detection limit of 10 µM (S/N = 3) and a high sensitivity of 44.6 ± 0.07 µA/mM·cm2 for the linear range between 0.05 and 0.2 mM. The biosensor response was stable for up to 6 weeks. Furthermore, the developed biosensor has been used to detect ethanol in alcoholic beverages with good results, suggesting its potential application in various fields, including fermentation processes and food quality control.

An ultra-sensitive electrochemical biosensor for the detection of procalcitonin in sepsis patients’ serum, using a Cu-BHT-based thin film

Procalcitonin (PCT) is a polypeptide produced by the parafollicular cells of the thyroid gland and serves as a vital marker for the diagnosis and treatment of sepsis and other infectious diseases, as well as multiple organ failure, due to its high expression levels in affected patients. This article reports on a highly sensitive electrochemical biosensor based on MOF composite materials, based on Cu-BHT, for detecting PCT levels. The surface of the glassy carbon electrode may have better charge transfer resistance owing to the nano-composite material made of Cu-BHT, chitosan, and AuNPs. At the same time, the anti-PCT antibody may also be covalently bonded to the composite material and measure PCT concentration using electrochemical impedance spectroscopy (EIS). The results of the investigation demonstrate that the sensor's response has excellent linear conjunction with the logarithm of PCT concentration under optimum circumstances. The detection limit (LOD) is 14.579 × 10−9 μg/mL, and the linear range of detection is 10−7 μg/mL to 10−1 μg/mL. Simultaneously, we successfully applied this method to detect serum PCT before and after treatment in different sepsis patients and compared it with chemiluminescence immunoassay. The findings indicate that the proposed method holds promising potential for timely diagnosis and treatment of sepsis patients.

Sensitive electrochemical biosensor for bisphenol A based on laccase immobilized on polypyrrole‐3‐carboxylic/Sb2O5/reduced graphene oxide hybrid nanomaterial

AbstractAn electrochemical biosensor for the bisphenol A determination was constructed onto a glassy carbon electrode modified with a hybrid nanostructure based on polypyrrole‐3‐carboxylic acid/Sb2O5/reduced graphene oxide and laccase enzyme. The hybrid nanostructure was characterized by X‐ray photoelectron spectroscopy (XPS), HR‐TEM, SEM microscopy, and electrochemical techniques. The biosensor displayed excellent response for bisphenol A determinations by differential pulse voltammetry, showing a theoretical detection limit of 9.9 nmol L−1 in the linear range of 0.1–1.0 μmol L−1. The biosensor demonstrated excellent selectivity in the determinations of bisphenol A in tap water without significant interference from other species, such as 17β‐estradiol, Estriol, Progesterone, Catechol, Hydroquinone, Ascorbic acid, and Dopamine. The biosensor also has presented an excellent performance in bisphenol A determinations in water, with recovery tax ranging from 99.6 to 101 %. Therefore, it can be used satisfactorily to detect bisphenol A in real samples.

Highly sensitive detection of L.monocytogenes using an electrochemical biosensor based on Si@MB/AuNPs modified glassy carbon electrode

L. monocytogenes (L. m) is one of the most common causes of foodborne diseases. To enable timely monitoring of L. m contamination, a highly efficient and sensitive electrochemical biosensor was developed for the precise determination of L.m. The biosensor was prepared by sequentially modifying glassy carbon electrode (GCE) with silicon methylene blue (Si@MB), gold nanoparticles (AuNPs) and aptamer (Apt). Differential pulse voltammetry (DPV) was used to quantify L.m. This biosensor could be finished in 1.5 h, which significantly reduced the detection time. The resulting electrochemical biosensor achieved good linear (102-107 CFU/mL) and excellent detection limit of 2.6 CFU/mL. Moreover, the novel strategy has been successfully applied to actual samples, with the recoveries ranging from 84.7% to116.0% in lettuce and from 80.0% to 110% in fresh-cut fruit. This biosensor shows great potential application in monitoring bacterial food contamination and infectious diseases.

Highly sensitive determination of putrescine in pork by an electrochemical biosensor constructed with unfolded hemoglobin and diamine oxidase

Based on the principle that diamine oxidase (DAO) catalyzes putrescine to produce H2O2 and unfolded hemoglobin (uHb) has the good catalytic ability to H2O2, a highly sensitive electrochemical biosensor for the determination of putrescine was constructed. The biosensor was prepared through immobilizing uHb on the surface of clay modified glassy carbon electrode (GCE) by physical adsorption method, and then using 2.5% glutaraldehyde as cross-linking agent to fix DAO. In order to achieve the best results, the concentration of DAO, the type of peroxidase, the stability of modified electrode and the test conditions were optimized. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize the preparation process of the sensor. Under the optimized conditions, the difference of reductive peak currents had good linear relationships with putrescine concentration in the ranges of 1.0 × 10−11-1.0 × 10−10 mol L−1 and 1.0 × 10−10-1.0 × 10−9 mol L−1 (r ≥ 0.997), respectively. The limit of detection (LOD) was 3.3 × 10−12 mol L−1 (S/N = 3). The developed electrochemical biosensor had good reproducibility, stability and sensitivity, and was successfully applied for the determination of putrescine in real pork samples.

Development of silver nanoparticles and aptamer conjugated biosensor for rapid detection of E. coli in a water sample.

A simple, rapid, and sensitive electrochemical biosensor based on a screen-printed carbon electrode (SPCE) was developed for onsite detection of E. coli in real time. This work analyzed the effect of aptamer conjugation and PBS buffer solution on the colloidal stability of the silver nanoparticles (AgNPs). Aggregations of the AgNPs after aptamer conjugation in PBS buffer were observed from the particle size distribution analysis. The AgNP-aptamer conjugation and its affinity towards E. coli (DH5α) were confirmed by UV-visible spectrophotometry, which showed a linear increment in the absorption with increasing E.coli concentration. The screen-printed carbon electrodes were modified by drop-casting of AgNPs, which were used as an effective immobilization platform for E. coli-specific aptamers. The modified electrode's surface modification and redox behavior were characterized using cyclic voltammetry. Finally, E. coli was detected using differential pulse voltammetry with an optimized incubation time of 15min. The developed biosensors showed a linear decrease in current intensity with an increase in the concentration of E. coli. The biosensor had a relative standard deviation (RSD) of 6.91% (n = 3), which showed good reproducibility. The developed biosensors are highly sensitive and have a limit of detection (LOD) as low as 150CFU/ml. The biosensor showed good selectivity for E.coli coli when comparing the signal response obtained for bacteria other than E.coli. Also, the biosensor was found stable for four weeks at room temperature and showed high recoveries from 95.27% to 107% during the tap water sensitivity validation.

Sensitive detection of SARS-CoV-2 spike protein based on electrochemical impedance spectroscopy of Fe3O4@SiO2–Au/GCE biosensor

Highly contagious COVID-19 disease is caused by a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which poses a serious threat to global public health. Therefore, the development of a fast and reliable method for the detection of SARS-CoV-2 is an urgent research need. The Fe3O4@SiO2–Au is enriched with a variety of functional groups, which can be used to fabricate a sensitive electrochemical biosensor by biofunctionalization with angiotensin-converting enzyme 2 (ACE2). Accordingly, we developed a novel electrochemical sensor by chemically modifying a glassy carbon electrode (GCE) with Fe3O4@SiO2–Au nanocomposites (hereafter Fe3O4@SiO2–Au/GCE) for the rapid detection of S-protein spiked SARS-CoV-2 by electrochemical impedance spectroscopy (EIS). The new electrochemical sensor has a low limit detection (viz., 4.78 pg/mL) and a wide linear dynamic range (viz., 0.1 ng/mL to 10 μg/mL) for detecting the EIS response signal of S-protein. The robust Fe3O4@SiO2–Au/GCE biosensor has high selectivity, stability, and reproducibility for the detection of S-protein with good recovery of saliva samples.

Development of metal free carbon catalyst derived from Parthenium hysterophorus for the electrochemical detection of dopamine

Parthenium hysterophorus, one of the seven most hazardous weeds is widely known for its allergic, respiratory and skin-related disorders. It is also known to affect biodiversity and ecology. For eradication of the weed, its effective utilization for the successful synthesis of carbon-based nanomaterial is a potent management strategy. In this study, reduced graphene oxide (rGO) was synthesized from weed leaf extract through a hydrothermal-assisted carbonization method. The crystallinity and geometry of the as-synthesized nanostructure are confirmed from the X-ray diffraction study, while the chemical architecture of the nanomaterial is ascertained through X-ray photoelectron spectroscopy. The stacking of flat graphene-like layers with a size range of ∼200–300 nm is visualized through high-resolution transmission electron microscopy images. Further, the as-synthesized carbon nanomaterial is advanced as an effective and highly sensitive electrochemical biosensor for dopamine, a vital neurotransmitter of the human brain. Nanomaterial oxidizes dopamine at a much lower potential (0.13 V) than other metal-based nanocomposites. Moreover, the obtained sensitivity (13.75 and 3.31 μA μM−1 cm−2), detection limit (0.6 and 0.8 μM), the limit of quantification (2.2 and 2.7 μM) and reproducibility calculated through cyclic voltammetry/differential pulse voltammetry respectively outcompete many metal-based nanocomposites that were previously used for the sensing of dopamine. This study boosts the research on the metal-free carbon-based nanomaterial derived from waste plant biomass.

Electrochemical biomimetic enzyme cascade amplification combined with target-induced DNA walker for detection of thrombin

Fe-N-doped carbon nanomaterials (Fe-N/CMs) were designed as a novel biomimetic enzyme with excellent peroxidase-like activity to achieve high-efficient enzyme cascade catalytic amplification with the aid of glucose oxidase (GOx), which was further combined with target-induced DNA walker amplification to develop a sensitive electrochemical biosensor for thrombin detection. Impressively, massive output DNA was transformed from small amounts of target thrombin by highly effective DNA walker amplification as protein-converting strategy, which could then induce the immobilization of functionalized nanozyme on the electrode surface to achieve the high-efficient electrochemical biomimetic enzyme cascade amplification. As a result, an amplified enzyme cascade catalytic signal was measured for thrombin detection ranging from 0.01pM to 1nM with a low detection limit of 3 fM. Importantly, the new biomimetic enzyme cascade reaction coupled the advantages of natural enzyme and nanozyme, which paved an avenue to construct varied artificial multienzymes amplification systems for biosensing, bioanalysis, and disease diagnosis applications.

Synergetic Effect of Three-in-One Nanocomposite Based on AuNPs and rGO-MWCNTs for Ultrasensitive Electrochemical Bio-Diagnostic Applications

A simple, two-step AuNPs/rGO-MWCNTs nanocomposite fabrication methodology, via drop-casting of rGO-MWCNTs on bare screen printed electrode (SPE) followed by electrodeposition of AuNPs through voltammetric scanning, without using any toxic reducing agents is reported. Each step of the synthesized nanocomposite is characterized using state-of-art spectroscopic and electrochemical methods. Our results demonstrate that the nanostructured AuNPs/rGO-MWCNTs/SPE showed synergistic effects of high electrocatalytic and electron transfer activity, high surface area to volume ratio, that confirms our synthesized material is the best material to fabricate a portable device for rapid, inexpensive, and sensitive electrochemical biosensors. For validation, the fabricated material subjected to electrochemical sensing of BSA protein. Our AuNPs/rGO-MWCNTs nanocomposite exhibited significant and good linear response over 2.5–12.5 μg ml−1 BSA concentration range, with sensitivity of 1.723 μAμg ml−1 and limit of detection (LOD) 0.23 μg/ml. This portable sensing system with enhanced performance demonstrates great potential for our synthesized AuNPs/rGO-MWCNs/SPE nanocomposite in application of point of care applications, and offers a universal and reliable platform for in biomarker detection.

An electrochemical PNA-based sensor for the detection of the SARS-CoV-2 RdRP by using surface-initiated-reversible-addition−fragmentation-chain-transfer polymerization technique

Coronavirus disease 2019 is one of the global health problems. Herein, a highly sensitive electrochemical biosensor has been designed to detect the RNA-dependent RNA polymerase (RdRP) of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) (SARS-CoV-2 RdRP). Herein, the surface-initiated reversible-addition−fragmentation-chain-transfer polymerization was used to amplify the electrochemical signal. To do that, the thiol-terminated peptide nucleic acid (PNA) probes were first immobilized on the surface of a screen-printed electrode modified with reduced graphene oxide-gold nanocomposite and then the fixed concentration of the SARS-CoV-2 RdRP was added to the electrode surface to interact with PNA probes. Subsequently, the Zr 4+ ions were added to interact with the phosphate groups of the SARS-CoV-2 RdRP. It allowed us to polymerase the ferrocenylmethyl methacrylate (FcMMA) and 4-cyano-4-(phenylcarbonothioylthio)-pentanoic acid on the SARS-CoV-2 RdRP chain. Since the poly-FcMMA has an electrochemical signal, the response of the PNA-based sensor to SARS-CoV-2 RdRP was increased in the range of 5–500 aM. The limit of detection was calculated to be 0.8 aM which is lower than the previous sensor for SARS-CoV-2 RdRP detection. The proposed PNA-based sensor showed high selectivity to the SARS-CoV-2 RdRP in the presence of the gene fragments of influenza A and Middle East respiratory syndrome coronavirus.

Characterization of Enzymatic Glucose Biosensors Based on A Glassy Carbon Electrode Modified with MoS2 Nanorods

: In this work, molybdenum disulfide (MoS2) nanorods (NRs) were prepared by a simple hydrothermal method. A sensitive electrochemical glucose biosensor was developed based on the immobilization of glucose oxidase (GOx) on MoS2 NRs modified glassy carbon electrode (GCE). The SnO2 NRs were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDS). SnO2 NRs have large specific area and can load large amounts of GOx molecules. The cyclic voltammetry (CV) of GOx/MoS2 NR/GCE exhibited a linear relationship between the peak current density of CV with glucose concentration in the range of 3.0 mM to 7.0 mM with the limit of detection (LOD) of 3.0 mM and high sensitivity of mA.mM. The parameters affecting the oxidation current density such as pH, temperature, GOx concentration were also investigated. This study demonstrates the feasibility of realizing inexpensive, reliable, and highly effective performance glucose biosensors using MoS2 nanorods.