Sensitive Detection Of Hydrogen Peroxide Research Articles

A facile one-pot synthesis of Au core flower surrounding with thin Co3O4 shell for highly sensitive detection of hydrogen peroxide

In this study, we described the facile synthesis of Au core flower surrounding with thin Co3O4 shell for enhanced electrochemical sensing of H2O2. Such core-shell structure was obtained by employing Au3+ to oxidize Co2+ under alkaline conditions for triggering the redox assembly process. The experiment results revealed that conductive Au core flower was surrounded by thin Co3O4 shell, and such structure not only accelerates electron transfer rate but also provides more effective active sites for H2O2 reaction, therefore making the sensor based on Au@Co3O4 exhibit an excellent performance for H2O2 analysis with a linear range of 0.2 μM-7.1 mM, a high sensitivity of 722.4 μA mM−1 cm-2 and a low detection limit of 0.06 μM. The excellent experimental results implied that Au@Co3O4 nanocomposite was a promising sensing material for electrochemical detection of H2O2.

S-doped reduced graphene oxide: a novel peroxidase mimetic and its application in sensitive detection of hydrogen peroxide and glucose.

This article presents a novel peroxidase mimetic by doping S atoms into reduced graphene oxide (rGO), which was synthesized through a facile hydrothermal reaction without any templates or surfactants. The peroxidase-like activity of S-doped rGO (S-rGO) is greatly boosted compared with the pristine rGO, demonstrating the peroxidase-like active sites are dominantly originated in sulfur-containing groups. The steady-state kinetic studies further indicate that S-rGO obeys the typical Michaelis-Menten curves and has a much smaller Michaelis constant (Km) for hydrogen peroxide (H2O2) and 3, 3', 5, 5'-tetramethylbenzidine (TMB). In view of the outstanding performance of S-rGO as a peroxidase mimetic, an efficient and sensitive colorimetric detection platform for H2O2 and glucose has been successfully established. The linear detection for H2O2 is obtained in a range of 0.1-1μM with an extremely lower detection limit of 0.042μM, and glucose can be measured in a linear range of 1-100μM, giving a detection limit of 0.38μM. This study not only provides a new avenue for the reasonable design of heteroatom-doped carbon-based nanomaterials but also offers meaningful reference for detecting the important biomolecules in biotechnology. Graphical abstract.

Functionalised liquid crystal microfibers for hydrogen peroxide and catalase detection using whispering gallery mode

ABSTRACT In this work, we demonstrate, for the first time, a whispering gallery mode (WGM) lasing-based liquid crystal (LC) soft-matter microfiber biosensor for real-time and sensitive detection of hydrogen peroxide and catalase. The functionalised nematic LC 4-cyano-4ʹ-pentylbiphenyl (5CB) microfibers served as both optical microresonators and sensing actuators. The resonance spectral shift in WGMs can be used as a stable and reliable indicator of analyte concentrations. The detection limits for hydrogen peroxide and catalase were as low as ~0.26 μM and ~1 ng/mL, respectively. Direct measurement of the WGM lasing spectrum of LC soft-matter microfibers can be expected as an alternative method to detect analytes without the need for polarised image analysis in current LC sensor systems. This research provides a preliminary basis for the development of soft-matter microfiber biosensors with high sensitivity for detecting biochemical molecules.

Facile Electrochemical Routes for the Composite Coating on Au Inverse Opals As Highly-Sensitive Enzyme-Free Biosensors

The highly sensitive detection of hydrogen peroxide (H2O2) is of practical importance due to its involvement in the biofunction and signal transduction of cells. Various electrochemical techniques have been explored and studied for its detection, which can be realized through either enzymatic or non-enzymatic sensors. Particularly, non-enzymatic biosensors have been in great demand because of their high stability, high reproducibility, and less susceptible to environmental factors compared to the enzyme-based approach. However, many of them display inferior sensitivity or poor selectivity. Hence, this work aims to develop a highly sensitive non-enzymatic biosensor through structural and compositional approaches. From a structural point of view, Au inverse opals are utilized as the sensor scaffold due to their intriguing porous structure. The spatial arrangement of these materials resembles a honeycomb structure, which provides a high specific surface area, efficient mass transport, and strong mechanical stability. In addition to the benefits brought by inverse opals, we further incorporate a composite coating based on Cu2O to decorate the scaffold surface. Through the combination of a well-ordered pore structure with additional functionalization by composite formation, a synergistic improvement could be realized. To fabricate such structural biosensors, an expedited self-assembly process exploiting electrophoresis technique is first adopted to produce a colloidal crystal template. Subsequent electrodeposition processes are performed to construct the Au inverse opals and relevant functional coatings. The sensing performance of these 3D sensors is then evaluated by the detection of H2O2 using cyclic voltammetric analysis. Their structural and compositional characterizations are conducted by SEM, EDX, XPS, and Raman spectroscopy.

Electrostatic Self‐Assembled Bracelet‐Like Au@Pt Nanoparticles: An Efficient Electrocatalyst for Highly Sensitive Non‐Enzymatic Hydrogen Peroxide Sensing

AbstractIn this study, a non‐enzymatic electrochemical sensor was developed based on bracelet‐like Au@Pt nanoparticles (B−Au@Pt NPs) and used for extremely sensitive detection of hydrogen peroxide (H2O2). The uniquely shaped B−Au@Pt NPs with a well‐defined core–shell structure were synthesized by a combination of seed‐mediated growth and electrostatic self‐assembly, especially using both sodium citrate (SC) and cetyltrimethylammonium chloride (CTAC). Through characterization by dynamic light scattering (DLS), high‐resolution transmission electron microscopy (HRTEM) and UV/Vis absorption spectra, it was evident that each Au NP was surrounded by several ultrasmall Pt NPs, all of which were detached from and lose contact with Au cores. Compared with sea urchin‐like Au@Pt NPs (S−Au@Pt NPs) synthesized only by a seed‐mediated growth, B−Au@Pt NPs presented obvious electrochemical response and exhibited substantially enhanced electrocatalytic activity towards H2O2 reduction. This H2O2 sensor, denoted as B−Au@Pt NPs/GCE (GCE=glassy carbon electrode), with enhanced sensitivity, has shown much better sensing performance than other sensors based on Pt reported earlier, particularly the high sensitivity of 882.2 μA mM−1 cm−2. Moreover, this non‐enzymatic electrochemical sensor based on B−Au@Pt NPs may offer the potential for sensitive detection of H2O2 in aquatic environmental monitoring.

Hierarchical Mo2C@MoS2 nanorods as electrochemical sensors for highly sensitive detection of hydrogen peroxide and cancer cells

Heterostructures hierarchically integrating various building blocks are promising to construct sensitive and versatile biosensing platforms. Herein, hierarchical Mo2C@MoS2 consisting of interlayer-expanded MoS2 nanosheets wrapping on Mo2C nanorods is developed as a sensitive and bifunctional electrochemical platform to detect cellular metabolism products (e.g., H2O2) and cancer cells. Utilizing the merits of one-dimensional Mo2C axis (e.g., high conductivity) and two-dimensional MoS2 nanosheets (e.g., enhanced *OH binding, highly exposed edge-sites, and tolerance against oxidative H2O2), the Mo2C@MoS2 afford a high sensitivity (1080 μA mM−1 cm-2) and a low detection limit (0.2 μM) for H2O2 sensing, superior to the most of materials free from enzymes and noble metals. Its outstanding tolerance to various interferents ensures the practical monitoring of the intracellular H2O2 amount in MDA-MB-231, a human breast cancer cell line, which provides an indirect but available detection of tumor cells. As a versatile sensing platform, moreover the Mo2C@MoS2 with rich surface amino groups performs well to directly and specifically detect MDA-MB-231 after immobilizing folic acid. This work is anticipated to open up new opportunities toward high-performance sensing materials via surface/interfacial engineering on heterostructures.

A simple enzyme-free SERS sensor for the rapid and sensitive detection of hydrogen peroxide in food.

A simple enzyme-free method based on surface-enhanced Raman scattering (SERS) was developed for the first time to detect H2O2 in food by etching a self-assembled film of silver nanoparticles (Ag NPs) on a glass substrate. H2O2 is able to oxidize Ag NPs to yield Ag+ ions; this process reduces the size of the Ag NPs and ultimately leads to a decrease in the SERS signal of the Raman probe. The intensities of the SERS spectra were strongly correlated with H2O2 concentration, which indicated that the Ag NP self-assembled SERS sensor can be successfully used for the quantitative analysis of H2O2. The main advantage of this SERS sensor is that it can directly detect H2O2 without introducing complex enzymatic reactions. This easy-to-operate and fast-response detection technology has great potential for the sensitive detection of H2O2 in food.

A simple and sensitive hydrogen peroxide detection with horseradish peroxidase immobilized on pyrene modified acid‐treated single‐walled carbon nanotubes

AbstractBACKGROUNDHydrogen peroxide (H2O2) plays an essential role in different reactions and mechanisms. Therefore, simple and sensitive detection of H2O2 becomes very important for both medical and environmental applications.RESULTSTwo‐step immobilization of horseradish peroxidase (HRP) on acid‐treated single‐walled carbon nanotubes (cut‐SWCNTs) was reported for the sensitive detection of H2O2 at physiological conditions. Cut‐SWCNTs were first synthesized using acid treatment and then coated onto screen‐printed electrodes (SPEs) for electrochemical optimization tests. Electrochemical characterization of cut‐SWCNTs coated CSPEs showed a linear relationship between current and increasing material loading. Also, the films demonstrated good reproducibility and stability. Immobilization of HRP was then achieved successfully using pyrene crosslinking chemistry on cut‐SWCNT coated SPEs. The detection of H2O2 was achieved with HRP/cut‐SWCNT/SPE electrode configuration using voltammetry technique. The constructed biosensor has sensitivity and apparent Michaelis–Menten constant (Km) values of 34.1 μA L mmol–1 cm−2 and 0.47 mmol L–1, respectively within the linear range of 20 to 600 μmol L–1 H2O2 concentration. The biosensor also showed a good degree of reproducibility and low cross‐contamination for tested interferences.CONCLUSIONH2O2 biosensor was successfully developed using cut‐SWCNTs and HRP enzyme establishing successful direct electrochemical communication between the enzyme and electrode using pyrene crosslinking chemistry. This study suggests that developed biosensors could be promising for H2O2 detection in medical and environmental applications. © 2019 Society of Chemical Industry

Cytochrome C-decorated graphene field-effect transistor for highly sensitive hydrogen peroxide detection

High-level in vivo reactive oxygen species (ROS) can damage many biomolecules via oxidative stress and play vital roles in the pathogenesis of several bodily disorders. Therefore, fast and sensitive monitoring strategies for trace ROS, such as hydrogen peroxide (H2O2), are of great significance. Herein, we present a highly sensitive field-effect transistor (FET) sensor based on single-layer graphene for trace hydrogen peroxide (H2O2) detection. Graphene and cytochrome c (Cyt c) were employed as the conductive substrate material and biomolecular receptor for H2O2 detection, respectively. High-efficiency charge transfer can be achieved by reliable electrical contact across the Cyt c/underlying graphene interface. The Cyt c/graphene FET platform exhibited hole-transport behavior with high conductivity and high sensitivity toward H2O2 with a detection limit of 100fM and rapid response time (<1s). Moreover, our sensor platform was able to specifically discriminate H2O2 from a series of interfering substances, such as dopamine, ascorbic acid, glucose, uric acid and glutamate. This result, therefore, demonstrates that the proposed Cyt c/single-layer graphene FET sensor could facilitate the high-efficiency charge transfer between the redox center of the Cyt c/graphene interface, indicating a promising application in future trace H2O2 or free radical biosensors.

Nanoporous platinum-copper flowers for non-enzymatic sensitive detection of hydrogen peroxide and glucose at near-neutral pH values.

Multimodal nanoporous PtCu flowers(np-PtCu) were prepared via a two-step dealloying strategy under mild conditions. The np-PtCu alloy possesses an interconnected flower-like network skeleton with multiscale pore distribution. This material was placed on a glassy carbon electrode where it shows outstanding detection performance towards hydrogen peroxide and glucose in near-neutral pH solutions. It can be attributed to the specific structure in terms of interconnected nanoscaled ligaments, rich pore openings and a synergistic alloying effect. Figures of merit for detection H2O2 assay include (a) a working voltage of 0.7V (vs. the reversible hydrogen electrode); (b) a wide linear response range (from 0.01 to 1.7mM), and (c) a low detection limit (0.1μM). The respective data for the glucose assay are (a) 0.4V, (b) 0.01-2.0mM, and (c) 0.1μM. The method is not interfered in the presence of common concentrations of dopamine, acetaminophen and ascorbic acid. Graphical abstract Multimodal nanoporous (np) PtCu alloy was prepared via a two-step dealloying strategy under mild conditions. Np-PtCu exhibits superior electrocatalytic activity. The assay is highly sensitive, selective, and it allows for along-term detection of H2O2 and glucose.

Synergistic Effect of Polyoxometalate and Single Walled Carbon Nanotubes on Peroxidase‐like Mimics and Highly Sensitive Electrochemical Detection of Hydrogen Peroxide

AbstractA novel Keggin‐type metatungstate hybrid POMs ((APy)6[H2W12O40])/carboxylic acid functionalized single‐walled carbon nanotube based amperometric sensing platform was successfully constructed for the sensitive detection of hydrogen peroxide. A simple and reliable procedure was implemented for the synthesis of new composite materials obtained by the covalent grafting of (APy)6[H2W12O40] onto the surface of (SWCNT‐COOH). The synergistic effect of single walled carbon nanotubes on the electrochemical behavior of (APy)6[H2W12O40] was evidenced through the increase of the oxidation and reduction peaks of the polyoxometalate. The sensitivity of hydrogen peroxide detection was increased by a factor of 38.5 in presence of SWCNTs, showing their high influence on peroxidase‐like mimics of (APy)6[H2W12O40]. The detection limit, the response time, the repeatability and the shelf lifetime were respectively 0.4 μM, 10 s, &lt;4%, 60 days.

Mesoporous gold nanoparticles supported cobalt nanorods as a free-standing electrochemical sensor for sensitive hydrogen peroxide detection

The performance of an electrochemical sensor for detecting small biomolecules can be greatly upgraded by properly controlling the chemical nanostructure of electrocatalyst. In this study, we successfully prepared a unique hierarchical three-dimensional nanoarchitecture based on gold nanocrystals supported cobalt nanorods on foam substrate via a facile synthesis approach. The Au-Co NRs/3DNF hybrid has been effectively utilized as a novel binder-free self-supported electrochemical sensor for hydrogen peroxide detection with wide linear detection range of 2–799 μM, low limit of detection of 1.42 μM, long-term stability, and very excellent selectivity. Besides, the hybrid precisely detected hydrogen peroxide in real samples. The achieved results can be explained by the synergistic effect produced by the combination between gold and one-dimensional Co NRs. In addition, the 3D nanostructure with self-supported ability, large surface area, and without the use of any polymer binder, leads to the enhanced electroactive sites, conductivity, electrolyte penetration, and ion diffusion, thereby promoting the sensing performance. The results imply that the hybrid may open a prospective way for developing electrochemical sensor towards efficient detection of hydrogen peroxide.

Novel peroxiredoxin-based sensor for sensitive detection of hydrogen peroxide

A series of genetically encoded sensors have been developed to detect the important signaling molecule H2O2 in living cells. However, more responsive and sensitive biosensors need to be developed. To address these demands, we used E. coli as a platform to develop a novel fluorescent H2O2 sensor, which we refer to as TScGP. This sensor employs a circularly permuted YFP (cpYFP) and is based on a redox relay between peroxiredoxin (Prx) and thioredoxin (Trx). Structurally, cpYFP is sandwiched between a fungal PrxA and a C-terminal cysteine mutated TrxA that can form a stabilized disulfide bond between PrxA and TrxA in response to H2O2. We confirmed that TScGP can be used for detecting exogenous H2O2 in the range of 0.5–5 μM with high selectivity and rapidly detecting H2O2 within 30 s in E. coli. To demonstrate an application, cellular H2O2 production by menadione was detected directly by TScGP. Our results demonstrated that using Prx-Trx combination as a sensing moiety is another strategy in designing H2O2 sensor with high performance.

Polyethyleneimine-AuNPs-copper protoporphyrin nanocomposite: a novel biosensor for sensitive detection of hydrogen peroxide in human serum

The detection of hydrogen peroxide (H2O2) is essential due to its extensive application in bio-medical and environment. Herein, we synthesized a novel polyethyleneimine-Au nanoparticle-copper protoporphyrin (PEI-AuNPs-CuPP) nanocomposite toward H2O2 detection. Ultraviolet-visible spectrometry (UV-vis), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR) were used to characterize this nanomaterial. Analytical performance of this proposed sensor was evaluated through differential pulse voltammetry (DPV) and cyclic voltammetry (CV). It was found surprisingly that the PEI-AuNPs-CuPP-based sensor exhibited sensing for H2O2 with a detection limit of 91.74 aM. In addition, this sensor displayed superb selectivity in the presence of dopamine (DA), ascorbic acid (AA), uric acid (UA), and glucose (Glu), which held satisfactory analytical performance for the detection of H2O2 in human serum samples, indicating a promising application in biological sample analysis.

Enhanced peroxidase-like activity of Fe@PCN-224 nanoparticles and their applications for detection of H2O2and glucose

Inspired by natural hemin structure, the fabrication of metal organic framework (MOF) nanomaterials constructed by metal ions and iron porphyrin for peroxidase mimicking has attracted extensive attention recently due to their high concentration of active sites and great channeling for substrate diffusion. Herein, by using benzoic acid as capping agent, we fabricated uniform PCN-224 nanoparticles (NPs), which were composed of Zr6 cluster and tetrakis(4-carboxyphenyl)porphyrin (H2TCPP). Then Fe(Ⅲ) ions were incorporated into the center of H2TCPP producing iron porphyrin unit similar to hemin serving as catalytic active site. The procedure of iron incorporation didn't change the size and morphology of PCN-224 NPs. The newly formed Fe@PCN-224 NPs possessed peroxidase-like activity with much lower Km values and higher Kcat values than most of peroxidase-like nanozymes as well as natural horseradish peroxidase (HRP), indicating their enhanced catalytic activity. Taking advantage of the excellent peroxidase-like activity of Fe@PCN-224 NPs, we successfully developed a colorimetric method for simple and sensitive detection of hydrogen peroxide and glucose in combination with glucose oxidase.

Cobalt Containing Zeolitic Imidazolate Framework Incorporated Electrospun Carbon Nanofibers As Free-Standing Film Sensor for Electrochemical Detection of Hydrogen Peroxide

Hydrogen peroxide (H2O2) is used as an oxidizer, bleaching agent and disinfectant in various fields such food processing, medicine and environment remediation. Further, H2O2 is also produced as by-product of biochemical processes in human body. Thus, detecting the trace quantities of H2O2 is of great significance. Among various detection techniques, electrochemical sensing offers relatively simple and fast detection where current signal generated from redox reaction of target analyte corresponds to its concentration. The performance of electrochemical sensors i.e. its sensitivity, lower limit and detection range depends on the type of materials utilized as electrocatalyst. Noble nanoparticles supported by nanocarbons are commonly used due to their higher sensitivity of detection, but their high cost and scarcity limit their widespread use [1]. Among low cost transition metals, cobalt nanoparticles derived from pyrolysis of metal organic framework (ZIF-67) are attractive materials for electrochemical H2O2 sensing having superior properties such as high surface area, regular porous structure and conductive carbon matrix. Various conductive nanocarbon supports can be utilized to uniformly disperse ZIF-67 nanocrystals. Carbon nanofibers owing to low-cost scalable synthesis are ideal candidates for such conductive support. Their 1D structure offers highly conductive pathways for electron transport and they can form hierarchal porous structures with ZIFs where the micropores(<2nm) of ZIF contain active sites for reduction of H2O2 and macropores (>50 nm) created by interconnection of carbon nanofibers minimizes mass transfer resistance of reactants and products to and from active sites. In this work, cobalt-carbon nanocomposites are synthesized by electrospinning of polymer precursor polyacrylonitrile (PAN) solution in dimethylformamide (DMF) solvent in which 30% ZIF-67 was uniformly dispersed. Electrospun polymer film was stabilized in air at 250oC and carbonized in argon (Ar) at 900oC to yield cobalt nanoparticles incorporated nitrogen-doped carbon nanofiber (ZIF-67/N-CNF) film. Amperometric tests on glassy carbon electrode (GCE) show that incorporation of ZIF-67 can improve sensitivity of N-CNF upto 3 times to 300 μA/ mM. cm2 for H2O2 detection. Carbonization of polymer film under 5% H2/Ar atmosphere resulted in further improvement of sensitivity to 475 μA/mM.cm2. Morphological and chemical properties were characterized to understand structure-property relationships. This sensor also demonstrated good stability and selectivity and was applied to milk and fruit juice samples to show their potential for real applications. ZIF-67/N-CNF film can be used as free standing sensor for H2O2 detection which minimize the complicated steps of ink preparation typically required for powder based electrocatalysts for testing sensing performance on GCE. Further, miniaturized screen-printed electrodes (SPE) modified by ZIF-67/N-CNF, were fabricated as H2O2 sensor to demonstrate low-cost portable detection. Reference: Riaz, Muhammad Adil, et al. "Ultralow-Platinum-Loading Nanocarbon Hybrids for Highly Sensitive Hydrogen Peroxide Detection." Sensors and Actuators B: Chemical (2019), 283, 304-311. (https://doi.org/10.1016/j.snb.2018.12.041)

Vertical copper oxide nanowire arrays attached three-dimensional macroporous framework as a self-supported sensor for sensitive hydrogen peroxide detection

A hierarchical nanostructure consisting of uniform copper oxide nanowires vertically grown on three-dimensional copper framework (CuO NWs/3D-Cu foam) was prepared by a two-step synthetic process. The uniform CuO NWs anchored onto the 3D foam exhibited outstanding electrocatalytic activity towards hydrogen peroxide reduction due to the unique one‐dimensional direction with its excellent catalytic activity and large surface area of 3D substrate, which enhanced electroactive sites and charge conductivity. As a result, a wide linear detection range of 1 µM–1 mM, good sensitivity of 8.87 µA/(mM ⋅ cm2), low detection limit of 0.98 µM, and rapid response time of 5 s to hydrogen peroxide were achieved under a working potential of −0.4 V in phosphate buffer solution (pH of 7.4). In addition, the CuO NWs/3D-Cu foam material showed excellent selectivity to hydrogen peroxide and good resistance against poisonous interferents, including ascorbic acid, dopamine, urea, uric acid, and potassium chloride. Furthermore, the CuO NWs/3D-Cu foam presented good reproducibility, stability, and accurate detection for hydrogen peroxide in real sample; therefore, it may be considered to be a potential free-standing hydrogen peroxide sensor in practical analysis applications.

A colorimetric assay for sensitive detection of hydrogen peroxide and glucose in microfluidic paper-based analytical devices integrated with starch-iodide-gelatin system

Microfluidic paper-based analytical devices (μPADs) for detection of hydrogen peroxide and glucose have been developed. The analytical performance of colorimetric detection using the conventional starch-iodine color reaction has been significantly improved by using gelatin as the surface modifier which retains the enzyme activity in the dry filter paper strip, improves antioxidability, as well as decreases the strong background signal. Under optimal conditions, the color intensities show a good linear relationship with glucose concentration ranging from 0.5 to 5 mM and hydrogen peroxide concentration from 0.5 to 6 mM, with the detection limit of 0.05 mM and 0.1 mM, respectively. In addition, the accuracy of colorimetric sensor has been successfully assessed in detecting glucose from real human serum samples and recovery value ranges from 95.7% to 97%, which are approaching to the glucose oxidase endpoint. The new colorimetric assay exhibits high sensitivity, good selectivity, acceptable stability and reproducibility. The present approach is promising for monitoring glucose for point of care diagnostic applications, especially in regions with resource-limited settings.

A Cork-Based Smart Biosensing System for Ethanol

Developing a new biosensing platform for the direct detection of analytes in beverages can offer significant, new opportunities for medical science, microbiology, nutrition studies, and the food industry. Cork stoppers are widely used for beverage bottles, and the stopper’s inner sides can be in direct contact with beverages. Here, we show for the first time the development of an amperometric biosensor on a cork with a two electrode configuration containing carbon nanotube as working electrode and Ag/AgCl as reference electrode. The sensor displays sensitive detection of hydrogen peroxide. Via the immobilization of alcohol oxidase, the sensor can detect ethanol. We anticipate that these results could offer exciting opportunities for the fundamental studies of non-conventional biosensors, as well as provide a wide range of applications in the food and beverage industry.

One-step rapid synthesis of fluorescent silicon nanodots for a hydrogen peroxide-related sensitive and versatile assay based on the inner filter effect.

In this work, a kind of environment-friendly and water-dispersible silicon nanodot (SiND) was rapidly synthesized by using the mild reagents (3-aminopropyl)triethoxysilane (APTES) and glucose. It was found that the fluorescence of the as-prepared SiNDs can be quenched obviously by permanganate due to the inner filter effect. Inspired by this finding, a novel fluorescent sensor for sensitive detection of hydrogen peroxide (H2O2) was developed through the oxidation-reduction reaction between permanganate and H2O2. The detection limit of H2O2 is down to 2.8 nM. Since H2O2 is an important molecule and involved in various studies, this sensor could be applied in various H2O2-related biological analyses. As a proof-of-application demonstration, a sensitive biosensor for glucose detection was constructed through the catalytic oxidation of glucose to generate H2O2. The as-constructed sensor showed good linear response to glucose over the range from 0.16 to 16 μM with a detection limit of 0.11 μM. Moreover, the biosensor can be readily extended to other sensors for different targets, which indicates the broad applications of the proposed sensing strategy in biomedical analysis.