Sensitive Detection Of Hydrogen Peroxide Research Articles

Nickel-doped cuprous oxide nanocauliflowers with specific peroxidase-like activity for sensitive detection of hydrogen peroxide and uric acid

Copper-based nanomaterials have the properties of mimetic enzymes and can be used as excellent candidates for colorimetric sensing due to their environmental friendliness, low cost, and high abundance. In this paper, Ni-doped Cu2O nano cauliflower (Ni-Cu2O) was synthesized for the first time and applied to the detection of H2O2 and uric acid (UA) in human serum and urine. It was found that the proportion of Ni incorporation controls the morphology and the catalytic effect of Ni-Cu2O. The catalytic mechanism was studied by X-ray photoelectron spectroscopy, free radical capture experiments, photoluminescence spectroscopy, and steady-state kinetic analysis, which verified the redox reactions involving electron transfer and active substances. The results showed that Ni-Cu2O could catalyze the formation of reactive oxygen species (•OH, O2•−, 1O2, h+) from H2O2, which could oxidize 3,3′, 5,5′-tetramethylbenzidine (TMB) to oxTMB, and the color changed from colorless to blue. The Michaelis-Menten constant (Km) and the maximum initial velocity (Vmax) of Ni-Cu2O were 1.8 mM and 15.2×10−8 M/s, respectively. Based on the excellent peroxidase-like (POD) activity of Ni-Cu2O, a colorimetric sensing platform combined with TMB was proposed to sensitively detect H2O2 and UA in a wide range, and the detection limits were as low as 0.17 μM and 0.22 μM, respectively. This study creates a platform for using the Cu-based cauliflowers as a biosensor to detect UA in the medical and biomedicine fields.

Surface Functionalization of Gold Nanoparticles Using Alkyne Derivatives: Applications in Chemical Sensing.

Colloidal gold nanoparticles (AuNPs) are important nanomaterials for chemical sensing and therapeutics. For their application, it is vital to develop a reliable and robust surface functionalization method that can be applied to diverse functional molecules and offer better stability under harsh biological conditions. Currently, thiol (SH) is the most commonly used functional group for forming stable covalent bonds with AuNPs. However, thiolated molecules typically require complicated preparation procedures, are susceptible to oxidation, and are not compatible with many electrophiles and reducing groups. In this study, we report that surface functionalization of AuNPs can be achieved using alkyne derivatives, which exhibit several advantages over classical thiolation and peptide-bond methods, including straightforward preparation of alkyne derivatives, rapid and simple conjugation in buffers and complex media, higher conjugation efficiency, long-term stability, and resistance to decomposition under harsh conditions. Several alkynylated biotin and fluorescein derivatives are prepared, and the alkynylated-AuNPs are characterized using a lateral flow assay, gel electrophoresis, and spectroscopy techniques to investigate the conjugation efficiencies, size distributions, protein interaction properties, and binding mode of the Au-alkyne bond. We also demonstrate that alkynylated-AuNPs can be used for the sensitive detection of hydrogen peroxide and streptavidin proteins.

Dual-mode optical biocompatible-sensor enabled by enzyme-like activity of UiO-66 (Zr) for ultra-sensitive ROS detection in vitro

The simple, effective and highly sensitive detection of hydrogen peroxide (H2O2), which belongs to the reactive oxygen species (ROS), at low concentrations plays an indispensable role in the field of environmental protection, biological research and safety. In this study, a dual-mode optical biosensor, UiO-66@OPD, was developed based on the inherent peroxidase mimicking activity of UiO-66 (Zr) and the optical reaction of ortho-phenylenediamine (OPD) by extending the π-system through oxidative coupling, prototropism and elimination to form OPDox, thereby exhibiting strong orangish absorbance and greenish fluorescence. The catalase-mimicking activity of UiO-66 (Zr) was demonstrated by the catalytic oxidation of methylene blue in the presence of H2O2. Moreover, the Michaelis-Menten kinetic model confirmed the intrinsic peroxidase-like activity of UiO-66@OPD as a modified MOFzyme. The synthesized UiO-66 (Zr) facilitated the oxidation of OPD to OPDox by degrading H2O2 to the hydroxyl radicals. During the oxidation process, the absorption peak at 415 nm and the fluorescence peak at 565 nm of the synthesized probe were significantly enhanced by increasing the H2O2 concentration. Moreover, a colorimetric and fluorometric ultrasensitive sensor shows a good linear relationship between the intensity enhancement and H2O2 concentration in the range of 0–600 nM for absorption and fluorescence spectra with R2 = 0.9772, and R2 = 0.9948, respectively. To demonstrate the biological performance and biocompatibility of UiO-66@OPD as a biosensor, MTT evaluation was performed for the three cell lines MCF-10 A, HEK293 and A549, indicating high biocompatibility and good cell viability for biological applications. Ultimately, this convenient, environmentally friendly, biocompatible and cost-effective catalase-mimicking-based sensor system will open a new perspective for the development of portable kite-based biosensors In vitro.

A Novel Enzyme-Based Electrochemical Biosensor for Sensitive Detection of Hydrogen Peroxide Based on the Fluorescent Peptide Self-Assembled Nanomaterials

A Novel Enzyme-Based Electrochemical Biosensor for Sensitive Detection of Hydrogen Peroxide Based on the Fluorescent Peptide Self-Assembled Nanomaterials

Electrochemical high sensitive detection of hydrogen peroxide based on platinum-palladium nano-enzyme modified microelectrode

Hydrogen peroxide (H2O2) is widely distributed in living organisms and the environment, and its concentration is closely associated with human health. Therefore, the development of an efficient and sensitive detection method for H2O2 is imperative. In this study, a PtPd nano-enzyme composite was electrodeposited onto a platinum wire microelectrode (ME) using a one-step electrochemical deposition method to fabricate an H2O2 electrochemical sensor (PtPd/ME) with exceptional performance. The successful modification of PtPd on the electrode surface was confirmed by scanning electron microscopy (SEM) and X-ray energy dispersive spectroscopy (EDS). Chronoamperometry (i-t) was employed to investigate various technical parameters including sensitivity, detection limit, detection range, reproducibility, and anti-interference capability. The results demonstrate that the sensor exhibits a sensitivity of 11.94 mA M-1 cm-2 with a detection limit of 0.034 μM and a wide linear range from 31.25 μM to 4.15 mM while also demonstrating excellent anti-interference properties. These superior performances can be attributed to the strong catalytic activity towards H2O2 provided by PtPd nanoparticles as well as the fast electron transfer rate facilitated by microelectrodes. Furthermore, this sensor effectively enables real-time monitoring of H2O2 released by ascorbic acid-stimulated vascular endothelial cells and demonstrates its potential for detecting H2O2 in contact lens care solutions. These findings provide valuable insights for food and drug testing, and demonstrate great potential for monitoring key pathological processes within living cells.

Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide-MXene-Multi-Walled Carbon Nanotubes Electrode.

Hydrogen peroxide (H2O2) is a signaling molecule that has the capacity to control a variety of biological processes in organisms. Cancer cells release more H2O2 during abnormal tumor growth. There has been a considerable amount of interest in utilizing H2O2 as a biomarker for the diagnosis of cancer tissue. In this study, an electrochemical sensor for H2O2 was constructed based on 3D reduced graphene oxide (rGO), MXene (Ti3C2), and multi-walled carbon nanotubes (MWCNTs) composite. Three-dimensional (3D) rGO-Ti3C2-MWCNTs sensor showed good linearity for H2O2 in the ranges of 1-60 μM and 60 μM-9.77 mM at a working potential of -0.25 V, with sensitivities of 235.2 µA mM-1 cm-2 and 103.8 µA mM-1 cm-2, respectively, and a detection limit of 0.3 µM (S/N = 3). The sensor exhibited long-term stability, good repeatability, and outstanding immunity to interference. In addition, the modified electrode was employed to detect real-time H2O2 release from cancer cells and cancer tissue ex vivo.

Tailored electrochemical biosensor with poly-diallydimethylammonium chloride-functionalised multiwalled carbon nanotubes/gold nanoparticles/manganese dioxide, and haemoglobin for sensitive hydrogen peroxide detection

A very sensitive electrochemical biosensor, with haemoglobin (Hb) as its basis, has been created to quantify hydrogen peroxide (H2O2), an essential marker in environmental monitoring, food safety, and medical diagnosis. The sensor uses a simple, eco-friendly preparation method. Hb was immobilised on manganese dioxide nanostructure/gold nanoparticles/poly-diallydimethylammonium chloride-functionalised multiwalled carbon nanotubes (PDDA-MWCNT/AuNP/MnO2), characterised using various techniques: amperometry, voltammetry, X-ray diffraction (XRD), and transmission electron microscopy (TEM). Nafion was used as a binder membrane to preserve the biological and electrochemical properties of the protein on the modified electrode. In comparison to earlier research, the novel biosensor had a lower detection limit (1.83 μM) and a limit of quantification (6.11 μM) (S/N = 3) for H2O2. It also exhibited notable reproducibility, long-term stability, and repeatability. It was effectively used to measure the amount of H2O2 in cow milk and orange juice, yielding recoveries in the order of 98.90–99.53 % with RSDs less than 5.0 %, which makes it a promising biosensor for food control.

A Novel MXene@MOF@Pt NPs-Based Enzyme-Free Electrochemical Sensor for Highly Sensitive Detection of Hydrogen Peroxide Released from Living Cells

Rapid and accurate detection of hydrogen peroxide (H2O2), an important reactive oxygen species (ROS) released from living cells, is of great significance for early diagnosis of tumors. Here, a high sensitive enzyme-free electrochemical sensor for the detection of H2O2 released from living cells was constructed based on MXene@ZIF-8@Pt NPs nanocomposites. Through the characterization of physical and chemical properties, it was observed that Pt NPs with excellent catalytic activity were uniformly supported on MXene@ZIF-8, which exhibited excellent conductivity and large specific surface area. Thanks to the significantly enhanced catalytic activity derived from the successful integration of MXene, ZIF-8 and Pt NPs, under the optimal conditions, the sensing platform based on MXene@ZIF-8@Pt NPs exhibited a wide linear range from 355.4 nM to 21.75 mM, with a limit of detection as low as 120.9 nM, while showing satisfactory reproducibility and selectivity. Furthermore, the developed electrochemical sensor enables real-time monitoring of H2O2 released from living Hela cells under N-formylmethionyl-leucyl-phenylalanine stimulation. Overall, the MXene@ZIF-8@Pt NPs developed in this article will become a promising candidate in monitoring physiological processes.

An electrochemical paper-based analytical device with facile carbon fiber-sewed electrodes for highly sensitive detection of hydrogen peroxide in real water

A novel and facile method by sewing low-cost fiber electrodes on paper is proposed to fabricate electrochemical paper-based analytical devices (ePADs). Prussian Blue (PB) and Ag/AgCl were deposited on carbon fibers to prepare the working and reference electrodes. The hydrophilic carbon fiber was employed as the counter electrode. All electrodes were sewed on the paper substrate. The linear voltammetry scanning (LSV) and physical characterization results showed that the physico-chemical characteristics of the prepared electrode were maintained after sewing. As a proof of concept, the ePAD fabricating by sewing was used to detect hydrogen peroxide (H2O2). The limit of detection (LOD) achieved 0.9 µM and the ePAD possessed high anti-interference ability to a variety of common ions and biological molecules. Additionally, the robustness of the ePADs against bending (error 6.2 %) manifested that the ePAD had excellent practical applicability. The good reproducibility (3.65 %) displayed by the current responses confirmed that this strategy can be profitably adopted to easily assemble ePADs in a highly flexible manner. In addition, a user-friendly reagent-free ePAD was proposed to determine the content of H2O2 in real water sample without treatment. The good recovery percentage (95.7–99.7 %) demonstrated the high feasibility of the reagent-free ePAD with facile carbon fiber-sewed electrodes in the detection of H2O2 from complex water samples. These results confirm that the sewing method is feasible and promising. This work provides a new insight into fabrication of highly sensitive and highly selective ePADs with fiber electrodes.

Development and Validation of a Customized Amplex UltraRed Assay for Sensitive Hydrogen Peroxide Detection in Pharmaceutical Water.

For clean-room technologies such as isolators and restricted access barrier systems (RABS), decontamination using hydrogen peroxide (H2O2) is increasingly attractive to fulfill regulatory requirements. Several approaches are currently used, ranging from manual wipe disinfection to vapor phase hydrogen peroxide (VPHP) or automated nebulization sanitization. Although the residual airborne H2O2 concentration can be easily monitored, detection of trace H2O2 residues in filled products is rather challenging. To simulate the filling process in a specific clean room, technical runs with water for injection (WfI) are popular. Thus, the ability to detect traces of H2O2 in water is an important prerequisite to ensure a safe and reliable use of H2O2 for isolator or clean room decontamination. The objective of this study was to provide a validated quantitative, fluorometric Amplex UltraRed assay, which satisfies the analytical target profile of quantifying H2O2 in WfI at low nanomolar to low micromolar concentrations (ppb range) with high accuracy and high precision. The Amplex UltraRed technology provides a solid basis for this purpose; however, no commercial assay kit that fulfills these requirements is available. Therefore, a customized Amplex UltraRed assay was developed, optimized, and validated. This approach resulted in an assay that is capable of quantifying H2O2 in WfI selectively, sensitively, accurately, precisely, and robustly. This assay is used in process development and qualification approaches using WfI in H2O2-decontaminated clean rooms and isolators.

Fabrication of a non-enzymatic electrochemical sensor based on magnesium oxide nanosheets for selective and sensitive detection of hydrogen peroxide in food samples

Fabrication of a non-enzymatic electrochemical sensor based on magnesium oxide nanosheets for selective and sensitive detection of hydrogen peroxide in food samples

Layered bimetallic hydroxide nanocage assembled on MnO2 nanotubes: A hierarchical porous sugar gourd-like electrocatalyst for the sensitive detection of hydrogen peroxide in food.

Hydrogen peroxide is used widely as a disinfection or bleaching additive during processing in the food industry. However, excessive residues of hydrogen peroxide in food have serious human health implications. In the present study, a novel electrochemical sensing electrode (MnO2/ZIF-67@LDH) with hierarchical porous sugar gourd-like structure was fabricated through a multi-step hydrothermal method using ZIF as the precursor. The unique porous nanocage structure of the sensing electrode provided multidimensional charge transfer channels and accelerated the electron transfer rate. As a hydrogen peroxide sensor, the electrode had two detection linear ranges of 1×10-3-4mmol L-1 and 4-8mmol L-1, and the detection limit was 0.26µmol L-1. The MnO2/ZIF-67@LDH sensor was also applied to determine the content of hydrogen peroxide in actual food samples of juice and milk, and satisfactory recovery were achieved. The present study provides a novel and effective design strategy for the construction of electrochemical sensing electrodes.

Colorimetric sensor based on biogenic nanomaterials for high sensitive detection of hydrogen peroxide and multi-metals

Hydrogen peroxide (H2O2) and heavy metals, which are among the wastes of the industrial sector, become a threat to living things and the environment above certain concentrations. Therefore, the detection of both H2O2 and heavy metals with simple, low-cost, and fast analytical methods has gained great importance. The use of nanoparticles in colorimetric sensor technology for the detection of these analytes provides great advantages. In recent years, green synthesis of nanomaterials with products that can be considered biowaste is among the popular topics. In this study, silver/silver chloride nanoparticles (Ag@AgCl NPs) were synthesized using the green synthesis method as an eco-friendly and cheap method, the green algae extract was used as a reducing agent. The characterization of Ag@AgCl nanoparticles and green algae extract was carried out with several techniques such as Transmission Electron Microscopy (TEM), UV–Visible spectrometry (UV–Vis), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction patterns (XRD) methods were used for characterization. According to TEM analysis, the Ag@AgCl NPs typically spherical in form and range in size from 4 to 10 nm, and UV–vis showed the formation of surface plasmon resonance (SPR) of the Ag@AgCl between 400 and 450 nm. In addition, its activity as a colorimetric sensor for hydrogen peroxide (H2O2) and multi-metal detection was evaluated. Interestingly, Ag/AgCl NPs caused different color formations for 3 metals simultaneously in the sensor study for heavy metal detection, and Fe3+, Cu2+, and Cr6+ ions were detected. The R2 values for H2O2, Fe3+, Cu2+, and Cr6+ were 0.9360, 0.9961, 0.9787, and 0.9625 the limit of detection (LOD) was 43.75, 1.69, 3.18, and 5.05 ppb (ng/mL), respectively. It was determined that Ag@AgCl NPs have the potential to be used as a colorimetric sensor for the detection of H2O2 and heavy metals from wastewater.

Novel chemiluminescent nanosystem for highly sensitive detection of hydrogen peroxide in vivo

The overproduction of hydrogen peroxide (H2O2) is closely associated with the progression of various diseases, and thus, it is an important diagnostic marker for in vivo molecular imaging. Although chemiluminescence (CL) imaging with L-012 as a CL agent has been employed to detect H2O2, the low reactivity of H2O2 with L-012 and the short CL emission time of L-012 limit its in vivo H2O2 detection applications. Herein, a novel Co2+-doped L-012/magnesium peroxide-based CL nanosystem (L-012/Co@MgO2) is designed. L-012/Co@MgO2 decomposes in aqueous solutions and releases L-012 and Co2+, and the highly reactive hydroxyl radicals produced by the Co2+-catalysed H2O2 decomposition under an inflammatory environment react with L-012 and yield strong and long-lasting CL emissions. In addition to visually detecting and quantitatively analysing H2O2, the biocompatible L-012/Co@MgO2 CL system against blood cells and major organs can successfully realise H2O2 detection in lipopolysaccharides-induced inflammatory disease models and in vitro screening of the H2O2 scavenging activity of antioxidants. The proposed CL nanosystem has potential applications for the biosensing and bioimaging of H2O2, which is highly produced in various diseases.

Enhancing catalytic performance of Fe and Mo co-doped dual single-atom catalysts with dual-enzyme activities for sensitive detection of hydrogen peroxide and uric acid

Single-atom catalysts (SACs) have attracted much attention due to their excellent catalytic activity, but the improvement of atomic loading which means that weight fraction (wt%) of metal atom was still facing great challenges. In this work, iron and molybdenum co-doped dual single-atom catalysts (Fe/Mo DSACs) was prepared for the first time by using the soft template sacrifice strategy, which improved significantly the atomic load and exhibited both the oxidase-like (OXD) activity and the dominant peroxidase-like (POD) activity. Further experiments reveal that Fe/Mo DSACs can not only catalyze O2 to generate O2•− and 1O2, but also catalyze H2O2 to generate a large number of •OH, which caused 3, 3′, 5, 5′-tetramethylbenzidine (TMB) to be oxidized to oxTMB, accompanied by the color changing from colorless to blue. The steady-state kinetic test showed that Michaelis–Menten constant (Km) values and the maximum initial velocity values (Vmax) of the POD activity of Fe/Mo DSACs were 0.0018 mM and 12.6 × 10−8 M s−1, respectively. The corresponding catalytic efficiency was tens of times higher than Fe SACs and Mo SACs, which proves that the synergistic effect between Fe and Mo has significantly improved the catalytic ability. Based on the excellent POD activity of Fe/Mo DSACs, a colorimetric sensing platform combined with TMB was proposed to realize the sensitive detection of H2O2 and uric acid (UA) in a wide range, with limits of detection as low as 0.13 and 0.18 μM, respectively. Finally, accurate and reliable results were obtained in the detection of H2O2 in cells, and of UA in human serum and urine.

An electrochemical biosensor based on CuFe PBA/MoS2 nanocomposites for stable and sensitive detection of hydrogen peroxide and carcinoembryonic antigen

An increasing number of electrochemical biosensors have been constructed to detect single bioactive substances with high sensitivity, but not multiple bioactive substances with different properties. Therefore, we incorporated Cu and Fe elements into Prussian blue to form Prussian blue analogues (PBA) to improve the electrochemical catalytic activity of Prussian blue. At the same time, MoS2 is used as the material substrate to increase the electrochemical active site of the composite. We have constructed a universal electrochemical sensing platform using composite materials (CuFe PBA/MoS2) as electrode modification materials for detecting two different types of representative cancer biomarkers, hydrogen peroxide (H2O2) and carcinoembryonic antigen (CEA). The universal electrochemical biosensor showed a significant linear response to both H2O2 and CEA with the lowest detection limits of 0.23 μM and 0.01 ng mL−1, respectively, and it had high selectivity, reproducibility, and stability. The universal electrochemical biosensor is successfully applied to detect H2O2 released from human breast cancer (MCF-7) cells and CEA expressed on the surface of human cervical cancer (HeLa) cells. The developed biosensor has potential in the dynamic detection of the flux of H2O2 and the expression level of CEA from living cells. The high sensitivity of this universal sensor provides a novel strategy for simultaneously detecting multiple cancer biomarkers.

Polydopamine-Based Colorimetric Superwettable Biosensor for Highly Sensitive Detection of Hydrogen Peroxide and Glucose

Polydopamine-Based Colorimetric Superwettable Biosensor for Highly Sensitive Detection of Hydrogen Peroxide and Glucose

Highly efficient tetrametallic PtNiCuCo alloy nanoparticles for sensitive detection of hydrogen peroxide

Highly efficient tetrametallic PtNiCuCo alloy nanoparticles for sensitive detection of hydrogen peroxide

Sulfur adlayer on gold surface for attaining H2O2 reduction in alkaline medium: Catalysis, kinetics, and sensing activities

The sensitive detection of hydrogen peroxide (HP) on Au surface is quite challenging owing to the potential dependent decomposition of HP. The current study investigated the development of chemisorbed sulfur adlayer onto the surface of the polycrystalline gold electrode to attain electroreduction of hydrogen peroxide in an alkaline medium using cyclic voltammetry (CV), chronoamperometry, and square wave voltammetry (SWV) measurement techniques. The sulfur adsorbed layer significantly reduces the decomposition of hydrogen peroxide onto the Au (pc) electrode surface allowing the hydrogen peroxide reduction process to proceed. The kinetic diagnosis suggests that the reduction is mass transfer controlled process where electron transfer is the rate determining step in the concerned reduction reaction. The presented electrochemical sensor exhibited high sensitivity and a quick amperometric response to HP in the concentration range of 10–2000 μM, with a detection limit of 6.0 μM.

Electroactive bacteria as whole-cell biosensor for sensitive detection of hydrogen peroxide

Hydrogen peroxide (H2O2) is a widely used strong oxidizer and has harmful effects on human health with excessive exposure. Herein, we proposed a simple and effective method for H2O2 detection by utilizing electroactive bacteria (EAB) as a whole-cell biosensor. The proposed method was achieved by utilizing the multiheme c-type cytochromes (central components of microbial extracellular electron transfer) of model EAB Shewanella oneidensis MR-1 to catalyze the oxidation of tetramethylbenzidine in the presence of H2O2. Owing to the excellent peroxidase-like activity of the multiheme c-cyts, this H2O2 detection method has a wide dynamic range between 0.1 and 1000 μM and a low detection limit of 0.17 μM. The proposed method, when coupled with glucose oxidase, displayed a high sensitivity (0.57 μM) and broad linear range (1 μM - 10 mM) for glucose. Moreover, this method has high specificity and good recovery for detecting H2O2-spiked real samples. Overall, this study has developed a novel biosensing platform that provides an alternative approach to H2O2 detection.