Sensitive Detection Of Dopamine Research Articles

Magnetic MOF composites for the electrocatalysis and biosensing of dopamine released from living cells.

Metal-organic frameworks (MOFs) with fit ligands and metals can be integrated into electrochemical biosensors for the detection of various biomolecules. In this study, we have synthesized novel magnetic MOF composites as electrocatalysts and constructed a novel biosensor for electrochemical detection of dopamine. The composites named Fe3O4@ZIF-8@AuNPs-COOH are synthesized through layer-by-layer assembly. They exhibit excellent stability and cooperative catalytic activity. In addition, green recycling is readily achieved through magnetizing/demagnetizing the electrode. The large specific surface area and ordered porous structures of the magnetic MOFs ensure good dispersion of gold nanoparticles, while the carboxyl group efficiently shields other redox-active interfering substances. The proposed electrochemical biosensor accomplishes the sensitive detection of dopamine in human serums and living cells. This study broadens the application of MOFs in electrochemical biosensing, validates the feasibility of biosensors for in vivo analysis, and provides new insights into green sensing.

Development of Novel Cobalt Tungstate Anchored on MCM-41 for Sensitive Detection of Dopamine in Human Urine Samples

In this study, MCM-41/CoWO4 nanocomposite has been used for electrochemical detection of dopamine neurotransmitters. The cobalt tungstate nanoparticles (CoWO4) were synthesized through facile hydrothermal method and MCM-41/CoWO4 nanocomposite was prepared by ultrasonication method. The as-synthesized MCM-41/CoWO4 nanocomposite was characterized by X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and Raman techniques. The electrochemical performance of the nanocomposite was examined using electrochemical impedance spectroscopy, cyclic voltammetry, and differential pulse voltammetry studies. The GCE/MCM-41/CoWO4 electrode exhibited a linear range, better sensitivity, and limit of detection (LOD) of 10–170 μM, 0.3361 μA μM−1 cm−2 and 7.2 nm, respectively. Moreover, the GCE/MCM-41/CoWO4 electrode demonstrated good repeatability, reproducibility, and stability. In addition to that, the real sample analysis was conducted using human urine with excellent recovery and RSD percentage.

One-Pot Hydrothermal Synthesis of mSiO2-N-CDs with High Solid-State Photoluminescence as a Fluorescent Probe for Detecting Dopamine

An effective fluorescent probe (mSiO2-N-CDs) was prepared by embedding N-CDs into mesoporous silica via a simple one-pot hydrothermal reaction and applied to the detection of dopamine (DA). Mesoporous silica not only provided a skeleton to prevent the aggregation of N-CDs but also a medium for the centrifugal collection of N-CDs, avoiding the need for dialysis and freeze-drying. The formation process, phase composition, morphology, and luminescence properties of the composite were studied in detail. The synthesized mSiO2-N-CDs possessed spherical morphology, a smooth surface, and a diameter of approximately 150 nm. The fluorescence results indicated that mSiO2-N-CDs emitted intense blue color fluorescence at 465 nm under the optimal excitation of 370 nm. Because the mesoporous silica effectively inhibited the self-quenching caused by the aggregation of N-CDs, the quantum yield of solid mSiO2-N-CDs powder reached 32.5%. Furthermore, the emission intensity of the solid mSiO2-N-CDs remained constant for 28 days. The good sensitivity and selectivity of mSiO2-N-CDs for DA enabled the establishment of a rapid, simple, and sensitive DA detection method. The linear range was 0–50 µM and the limit of detection was calculated to be 107 nM. This method was used for the determination of DA in urine, with recovery rates ranging between 98% and 100.8%. In addition, the sensing mechanism was characterized by fluorescence lifetime decay and UV–VIS spectral analysis.

A colorimetric/electrochemical sensor based on coral-like CuCo2O4@AuNPs composites for sensitive dopamine detection.

As a central neurotransmitter, DA (dopamine) plays a vital part in human metabolism, and its accurate detection is of great significance in disease diagnosis. In this work, we used Cu/Co bimetallic metal-organic frameworks (MOFs) as templates and gold nanoparticles (AuNPs) to construct novel nanocomposite coral-like CuCo2O4@AuNPs with strong peroxidase activity and electrochemical response. The coral-like CuCo2O4@AuNPs showed excellent peroxidase activity, and the Km value was as low as 0.358mM. In the presence of H2O2, the colorless substrate 3,3',5,5', -tetramethylbenzidine (TMB) can be catalytically oxidized into a blue product. Simultaneously, coral-like CuCo2O4@AuNPs, as an electroactive substance, possess strong electrocatalytic activity, which enhances the electron-transfer rate and promotes excellent current response. In the presence of DA, coral-like CuCo2O4@AuNPs can catalyze the oxidation of DA to dopaquinone, which further enhances the electrochemical signal. In addition, DA captures hydroxyl radicals and inhibits the oxidation of TMB, resulting in an obvious color change (blue turns colorless) and realizing colorimetric detection with the naked eye. On this basis, we successfully established a dual-mode colorimetric/electrochemical sensor using coral-like CuCo2O4@AuNP nanocomposites as a dual-signal probe. Combining colorimetric and electrochemical detection, the sensor achieved a wide linear range (0-1mM) and a low detection limit (0.07μM) for DA concentration. It was also successfully used for the detection of DA in human serum and urine with good results. In summary, this work provides an intuitive, economical, sensitive, and promising platform for DA detection.

MXene‐Coated Liposome‐Quercetin‐Functionalized Gold Nanoparticles for Electrochemical Detection of Dopamine

This study reports a novel electrochemical approach for selective dopamine (DA) sensing. DA is a potential neurological biomarker for human aging and other neurodegenerative disorders, particularly Alzheimer's disease. The sensor is constructed using titanium‐based MXene and quercetin (Qu)‐loaded liposome functionalized with gold nanoparticles (AuNPs) (MXene/Lip‐Qu@AuNPs). MXene/Lip‐Qu@AuNPs is coated on a glassy carbon electrode (GCE) for DA sensing. The MXene provides high conductivity for electrochemical measurements of DA. The modified GCE is employed for DA sensing against various interferants, including ascorbic acid, aspartic acid, arginine, and cysteine. The proposed MXene/Lip‐Qu@AuNP‐based GCE exhibits sufficient detection limits of 0.35 and 0.72 μM for DA sensing using cyclic voltammetry and differential pulse voltammetry methods within a linear concentration range of 1–10 μM, respectively. The proposed sensor is used to detect DA in human serum samples. Hence, the proposed novel sensor is highly convenient and provides highly selective and sensitive DA detection.

Nanozyme catalyzed-SERRS sensor for the recognition of dopamine based on AgNPs@PVP with oxidase-like activity

Dopamine (DA), as one of the most significant neurotransmitters, is closely related to several diseases. Achieving rapid and sensitive detection of DA remains a challenge. Herein, we proposed a simple, fast, and sensitive method for DA recognition based on surface-enhanced resonance Raman scattering (SERRS) technique. The synthesized silver nanoparticles coated with polyvinylpyrrolidone (AgNPs@PVP) with oxidase activity could not only oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) directly to produce a blue oxidation state TMB (oxTMB) but also could be used as the SERS substrate to generate a strong SERRS signal. When DA was added to the above system, the blue color faded along with the decrease in the SERRS signal. The change value of SERRS intensity was in proportion to the concentration of DA in the range of 0.1–10 μM with a limit of detection of 40 nM. This method presented great potential for the recognition of DA-related diseases.

LuMA-Functionalized Thermosensitive Hydrogel: A Versatile and Robust Dopamine-Triggered Platform for Diverse Biomolecules Sensing.

It is of great significance for the analysis of multiple biomarkers because a single biomarker is difficult to accurately achieve early diagnosis, disease course monitoring, and prognosis evaluation. Herein, a luminescence thermosensitive hydrogel was synthesized by radical polymerization using a methacrylic acid derivative monomer of luminol (LuMA) as luminescent, N-isopropylacrylamide (NIPAM) as thermosensitive monomer, and acrydite-oligonucleotides [dopamine (DA) aptamer, DNA C1, and DNA C2] as recognition elements. The combined DA based on the affinity interaction between the DA and the aptamer on the hydrogel polymer chain was electrochemically oxidized to dopamine quinone during the electrochemiluminescence (ECL) scanning, which effectively quenched the ECL signal of LuMA due to the resonance energy transfer (RET). In addition, the thermosensitive hydrogel showed swelling-collapse characteristics when the temperature was below and above the volume phase transition temperature. Undergoing the collapse process initiated by the temperature, the RET efficiency was further enhanced due to the shortened distance between the energy donor and acceptor, showing a 1.4 times signal amplification and achieving sensitive detection of DA with a limit of detection (LOD) of 1.7 × 10-10 M. For a proof of concept application, coupled with the target-induced release of DA from the DNA-magnetic beads bioconjugations based on duplex-specific nuclease (DSN)-assisted target recycling amplification strategy and DNAzyme cleavage reaction, this ECL-RET approach was successfully used to evaluate multiple targets including miRNA-141 and MUC1 with the LOD of 2.5 aM and 1.6 fg/mL, respectively. The excellent performances of the versatile and robust ECL-RET hydrogel in multiple target sensing showed potential applications in clinical diagnosis and disease therapeutic assay.

Facile fabrication of Fe-Fe3C nanoparticles decorated with carbon nanotubes for sensitive dopamine detection

In this study, we present an electrochemical sensor with superior sensitivity and selectivity for dopamine (DA) detection. The sensor was created using a nanocomposite of iron-iron carbide nanoparticles decorated with carbon nanotubes (Fe-Fe3C@CNTs) to modify a glassy carbon electrode (GCE). The Fe-Fe3C@CNTs nanocomposite can be synthesized by a facile microwave-assisted synthesis method with just adopting ferrocene and carbon fiber as raw material and heat source, respectively. The electron microscopy (EM) results indicate that the Fe-Fe3C nanoparticles encapsulated with graphitic layer were decorated with CNTs. An investigation of the electrochemical responses of the Fe-Fe3C@CNTs/GCE sensor to DA was performed using both cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Notably, in ideal experimental settings, the sensor demonstrated a minimal limit of detection (LOD) of 0.01 μM within a range of linearity from 0.05 to 40 μM, even under a high concentration of uric acid (UA) interference. Moreover, the utility of the produced Fe-Fe3C@CNTs/GCE sensor was further validated through successful DA testing in urine samples of human.

Vancomycin-Stabilized Platinum Nanoparticles with Oxidase-like Activity for Sensitive Dopamine Detection.

The development of efficient, reliable, and sensitive dopamine detection methods has attracted much attention. In this paper, vancomycin-stabilized platinum nanoparticles (Van-Ptn NPs, n = 0.5, 1, 2) were prepared by the biological template method, where n represented the molar ratio of vancomycin to Pt. The results show that Van-Pt2 NPs had oxidase-like activity and peroxidase-like activity, and the mechanism was due to the generation of reactive oxygen 1O2 and OH. Van-Pt2 NPs exhibited good temperature stability, storage stability, and salt solution stability. Furthermore, Van-Pt2 NPs had almost no cytotoxicity to A549 cells. More importantly, the colorimetric detection of DA in human serum samples was performed based on the oxidase-like activity of Van-Pt2 NPs. The linear range of DA detection was 10-700 μM, and the detection limit was 0.854 μM. This study establishes a rapid and reliable method for the detection of dopamine and extends the application of biosynthetic nanoparticles in the field of biosensing.

Electrochemical sensor based on three-dimensional Skeleton/Skin ink for the ultrasensitive detection of dopamine released from neural cells

The development of a miniaturized and low-cost platform for the simultaneous and high-sensitive detection of the nervous system metabolites has gained great interest for early diagnosis and prognostic monitoring of some diseases. Herein, a three-dimensional (3D) interpenetrating hydrogel ink synthesized through a simple hydrothermal assembly way was utilized for the sensitive detection of dopamine released from neural cells in vivo. Carbon nanotubes (CNTs) were utilized as the structural framework, while reduced graphene nanosheets were employed as the outer covering, resulting in the formation of a “skeleton/skin” structure. The attachment of hydrophilic IL molecules onto the graphene nanosheets confers distinctive hydrophilic traits on the resulting nanocomposite material. Consequently, this facilitates its effortless conversion into freestanding paper-like material via printing. The modified electrode was proved to be efficient in electrochemical detection of dopamine by means of DPV, despite the existence of interfering substances like ascorbic and uric acid. With a sensitivity of 41.86 μA μM−1 cm−2 and a low detection limit of 2 nM (S/N = 3), the paper electrode exhibited a linear response ranging from 0.01 to 50 μM. Furthermore, the rGO-CNT-IL was used to determine dopamine levels in the neural cells. The modified paper electrode can also be potentially applied for further drug detection, health monitoring and other biological applications.

Sulfur-vacancy-enriched MoS2-CNTs with highly dispersed Au particles for sensitive dopamine detection

Introducing targeted surface defects sites to regulate the electronic structure is a key method to enhance the electrocatalytic activity of materials. This study reports an electrochemical dopamine sensor using sulfur vacancy (Sv) rich MoS2-CNTs with coaxial hierarchical structure and highly dispersed active gold particles (Au-Sv-MoS2-CNTs). MoS2 with sulfur vacancies expands the layered structure beyond the MWCNT's cylinder rather than being restricted to its surface and has active Au deposition sites along the edges. With optimal conditions, the proposed coaxial, hierarchical-structured Au-Sv-MoS2-CNTs show remarkable detection performance for the rapid detection of DA at concentrations as low as 2 nM in phosphate buffered saline (PBS). In addition, the sensor selectivity and anti-interference qualities are evaluated using chronoamperometry. Furthermore, it successfully detected the analyte from injection of dopamine hydrochloride and bovine serum samples with recovery rates ranging from 99.3% to 102.6% and relative standard deviations (RSD) less than 5%. Density functional theory (DFT) simulations demonstrate that the modifications of Sv and Au NPs could tune the electronic structure of the sensor and facilitate the adsorption and electrocatalytic reaction of DA on Au-Sv-MoS2-CNTs. This study reveals that vacancy engineering and morphology control are emerging strategies for the fabrication of biosensors.

Microfluidic flow injection analysis system for the electrochemical detection of dopamine using diazonium-grafted copper nanoparticles on multi-walled carbon nanotube-modified surfaces

In this proof-of-concept study, a microfluidic flow injection analysis (FIA) system was developed using multi-walled carbon nanotube-modified screen-printed carbon electrodes (CNTSPEs) that were modified with copper nanoparticles (CuNPs) following the electrodeposition of the diazonium salt of 4-aminothiophenol to form 4-thiophenol-conjugated CuNPs (CuNPs-CNTSPE). Transmission electron microscopy (TEM), atomic force microscopy (AFM), and scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS) were used to characterize the size of CuNPs, morphology and elemental analysis of CuNPs-CNTSPE, respectively. Using electrochemical impedance spectroscopy (EIS), the charge-transfer resistance (Rct) of CuNPs-CNTSPE was found to be 20-fold lower than that of CNTSPE. The CuNPs-CNTSPE displayed an oxidation peak for dopamine at −0.08 V which is ∼80 mV lower than the one detected using CNTSPE. The modified electrode was used in microfluidic flow injection analysis and offline systems for sensitive detection of dopamine (DA). The pH, flow rate, loop volume, concentration, and type of surfactant were all optimized for on-chip detection. Under the optimal conditions, using phosphate electrolyte solution (pH 6) containing 0.05% (w/v) Tween 20® as the carrier at a flow rate of 0.6 mL min−1 and a loop volume of 50 μL, the calibration curve was linear from 1.5 to 500 nM with a limit of detection of 0.33 nM. This technique was used for the successful detection of DA in real samples with recovery ranging from 96.5% to 103.8%. The microfluidic FIA system described here has the potential to be used as an electrochemical point-of-care device for rapid DA detection with high sensitivity and reproducibility.

Rich‐defect carbon nanotubes for highly sensitive detection of Dopamine

AbstractDopamine (DA) plays an essential role in the central nervous, renal, hormonal and cardiovascular systems. Various modified carbon nanotubes (CNT)‐based dopamine sensors have been reported, but inexpensive, highly sensitive plain CNT‐based ones are seldom studied. In this work, a facile and inexpensive CNT‐based DA sensor is made by rich‐defect multi‐walled carbon nanotubes (RD‐CNT) via an ultrasound method. The defect and elemental states of the RD‐CNT are systematically studied by transmission electron microscopy (TEM), high‐resolution transmission electron microscopy (HR‐TEM), Raman spectroscopy, X‐ray powder diffraction (XRD) and X‐ray‐photoelectron spectroscopy (XPS). Results show that massive holes and cracks exist in RD‐CNT. The level of defects increases from the additional exposed edges. The electrochemical characterizations indicate that the electrochemical sensor has the highest sensitivity of 438.4 μA/(μM ⋅ cm2) among all carbon materials‐based DA sensors while well meeting the clinically required detection range and selectivity. The DA sensor was further used to detect live healthy human serum and live PC12 cells with satisfactory results, thus holding great promise for an inexpensive but sensitive DA sensor in practical applications of clinical diagnosis and biological research.

A novel electrochemical sensor based on DUT-67/ZnCo2O4-MWCNTs modified glassy carbon electrode for the simultaneous sensitive detection of dopamine and uric acid

Uric acid and dopamine are closely related to the health of the organism, so it is important to determine them simultaneously and efficiently. In this paper, the metal-organic framework DUT-67 was in situ grown on the surface of bimetallic oxide ZnCo2O4 nanoflowers, and then ultrasonically compounded with multi-walled carbon nanotubes (MWCNTs) to prepare DUT-67/ZnCo2O4-MWCNTs composite. The prepared composite was characterized by XRD, SEM, XPS, FTIR, BET and other techniques. Finally, an electrochemical sensor based on DUT-67/ZnCo2O4-MWCNTs composite modified glassy carbon electrode for simultaneous detection of dopamine (DA) and uric acid (UA) was fabricated. The introduction of ZnCo2O4 into the composite efficiently overcame the disadvantage of the easy aggregation of DUT-67 and produced a synergistic effect with MWCNTs. The electron transfer rate between the electrode and the electrolyte solution was accelerated, and the detection signals of the target molecules were amplified. After characterizing the electrochemical properties of the composite electrode, it was found that the constructed electrochemical sensor showed low detection limits for DA and UA, which were 12 nM and 8.7 nM, respectively, and the wide linear range of 1.0–180.0 μM. Besides, the sensor had excellent reproducibility, repeatability, anti-interference and stability. The proposed sensor was also successfully used to determine the contents of DA and UA in dopamine injection and urine samples with relatively satisfactory recovery rates. This work not only provides a new strategy for the preparation of MOF modified electrodes, but also has a potential application prospect in bioassays.

Remarkably Enhanced Luminol/H2O2 Chemiluminescence with Excellent Peroxidase-like Activity of FeCoNi-based Metal-Organic Xerogels for the Sensitive Detection of Dopamine.

Metal-organic gels (MOGs) are a category of metal-organic smart soft materials with large specific surface areas, loose porous structures, and open metal active sites. In this work, trimetallic Fe(III)Co(II)Ni(II)-based MOGs (FeCoNi-MOGs) were synthesized at room temperature via a simple and mild one-step procedure. Fe3+, Co2+, and Ni2+ were the three central metal ions in it, while 1,3,5-benzenetricarboxylic acid (H3BTC) served as the ligand. The solvent enclosed in it was then removed by freeze-drying to get the corresponding metal-organic xerogels (MOXs). The as-prepared FeCoNi-MOXs have excellent peroxidase-like activity and can significantly enhance luminol/H2O2 chemiluminescence (CL) by more than 3000 times, which is very effective compared with other reported MOXs. Based on the inhibitory effect of dopamine on the CL of the FeCoNi-MOXs/luminol/H2O2 system, a simple, rapid, sensitive, and selective CL method for dopamine detection was established with a linear range of 5-1000 nM and a limit of detection of 2.9 nM (LOD, S/N = 3). Furthermore, it has been effectively used for the quantitative measurement of dopamine in dopamine injections and human serum samples, with a recovery rate of 99.5-109.1%. This research brings up prospects for the application of MOXs with peroxidase-like activity in CL.

A SERS Composite Hydrogel Device for Point-of-Care Analysis of Neurotransmitter in Whole Blood

Point-of-care analysis of neurotransmitters in body fluids plays a significant role in healthcare improvement. Conventional approaches are limited by time-consuming procedures and usually require laboratory instruments for sample preparation. Herein, we developed a surface enhanced Raman spectroscopy (SERS) composite hydrogel device for the rapid analysis of neurotransmitters in whole blood samples. The PEGDA/SA composite hydrogel enabled fast separation of small molecules from the complex blood matrix, while the plasmonic SERS substrate allowed for the sensitive detection of target molecules. 3D printing was employed to integrate the hydrogel membrane and the SERS substrate into a systematic device. The sensor achieved highly sensitive detection of dopamine in whole blood samples with a limit of detection down to 1 nM. The whole detection process from sample preparation to SERS readout can be finished within 5 min. Due to the simple operation and rapid response, the device shows great potential in point-of-care diagnosis and the monitoring of neurological and cardiovascular diseases and disorders.

Defect engineering of PdMo metallene for sensitive electrochemical detection of dopamine

Reasonable design and construction of high-performance catalysts are desirable for improving the sensitivity of electrochemical sensors, but still remain a great challenge. Herein, ultrathin PdMo metallene with defects (D-PdMo) is fabricated and employed as an electrocatalyst to construct electrochemical sensors for the sensitive determination of small biomolecules. Taking advantage of the change of electronic structure, conductivity and electrochemical active surface areas caused by the defect engineering, the resultant D-PdMo metallene exhibits a much better electrocatalytic ability to dopamine (DA) than PdMo metallene. Furthermore, the proposed electrochemical sensor realizes sensitive DA detection in human blood serum and cells. Our work not only verifies the feasibility of introducing defects into metallene to enhance catalytic performance but also broadens the application of metallene in the electrochemical sensors for monitoring biomolecules in living organisms.

Ionic Covalent Organosilicon Polymer Nanosheet for Selective and Sensitive Detection of Dopamine

Ionic Covalent Organosilicon Polymer Nanosheet for Selective and Sensitive Detection of Dopamine

MnO4--Triggered Immediate-Stable Real-Time Fluorescence Immunosensor with High Response Speed and Low Steady-State Error.

Real-time chemical and biological sensing in vitro is important for application in health and environmental monitoring. Thus, a more rapid and stable detection method is urgently needed. Herein, an immediate-stable real-time fluorescent immunosensor with a high response speed (∼100%, <1 s) and approximately zero steady-state error is constructed. The developed sensor is based on the MnO4--triggered in situ immediate-stable fluorogenic reaction between dopamine and orcinol monohydrate to produce azamonardine (DMTM). The obtained DMTM is identified and characterized by high-resolution mass spectrometry, 1H NMR spectroscopy, 13C NMR spectroscopy, and theoretical calculations. The present sensor achieves a highly sensitive detection of dopamine (DA) with a limit of detection (LOD) of 10 nM as well as alkaline phosphates (ALP) with an LOD of 0.1 mU/mL by using orcinol monohydrate phosphate sodium salt as a substrate. As a proof of concept, ALP-triggered fluorescence ELISA using cardiac troponin I (cTnI) as a model antigen target is further constructed. The developed real-time sensor achieves the detection of cTnI with an LOD of 0.05 ng/mL. Moreover, the sensor proposed by us is successfully applied to assess the cTnI level in clinical serum specimens and yields results consistent with those obtained by the commercial ELISA method. The immediate-stable real-time fluorescence immunosensor provides a promising and powerful platform for the trace detection of biomolecules in clinical diagnosis.

Vertical growth of leaf-like Co-metal organic framework on carbon fiber cloth as integrated electrode for sensitive detection of dopamine and uric acid

Application of metal organic framework (MOF) in electrochemical sensors usually suffer from poor conductivity, low electrocatalysis, material aggregation and complex electrode fabrication. Here, leaf-like Co-MOF (L-Co-MOF) vertically grown on carbon cloth (CC) was prepared as integrated electrode for electrochemical detection. The L-Co-MOF/CC with interlaced Co-MOF nanosheets as building blocks greatly reduces aggregation, provides large surface area and highly exposed active sites to interact with analyte. The synergy between L-Co-MOF and CC endows the composite greatly enhanced electrocatalysis on oxidation of dopamine (DA) and uric acid (UA) compared with those of ordinary polyhedral Co-MOF or 2D Co-MOF grown in solution. The L-Co-MOF/CC can be directly used as working electrode without further modification, and it achieved the separate detection of DA and UA in the range of 5.6 nM-70 μM, and 21 nM-350 μM, respectively. Simultaneous detection of DA and UA was also performed with a LOD of 2.5 nM for DA and 7 nM for UA, respectively. The sensor with high reproducibility and good anti-interference properties also achieved practical sensing of DA and UA in serum with recoveries of 83.3–104.0%. Such integrated 2D-MOF-carbon material composite with unique structure and high electrocatalysis hold great promise in electrochemical device.