Seminal Characteristics Research Articles

Manual Collection and Semen Characterization in a West Indian Manatee (Trichechus manatus).

Limited information is available regarding male reproductive physiology in West Indian manatees (Trichechus manatus). Currently, only basic ultrastructural and morphometric descriptions of the spermatozoon exist; however, there are no reports evaluating any seminal characteristics in this species. Therefore, the aim of the study was to fill current gaps in knowledge regarding semen parameters in West Indian manatees by collecting and characterizing multiple ejaculate samples from a single, adult West Indian manatee. Samples were analyzed for the following semen parameters: volume, agglutination, pH, osmolality, viscosity, concentration, total sperm number, motility and kinematic parameters, morphology, plasma membrane integrity, acrosome integrity, chromatin maturation, and chromatin condensation. All macroscopic semen parameters varied to some extent between samples. Total and progressive motility was high for ejaculates 2 to 5, exceeding 97 and 89%, respectively; however, these parameters decreased dramatically throughout ejaculates 6 and 7. Across all samples, curvilinear velocity, straight-line velocity, and average pathway velocity represented the largest significant differences (p < 0.001) between each of the progression velocity subgroups (rapid, medium, slow). Sperm characteristics, including acrosome integrity (79.8%), chromatin condensation (93.1%), and chromatin maturation (99.5%) were very high; however, high numbers of morphologically abnormal sperm were present (52.9%) and plasma membrane integrity was low (45.1%). These results are the first of their kind for this species and suggest high semen quality, based on multiple ejaculates, in this male West Indian manatee.

Testicular transcriptome analysis under the dietary inclusion of l-carnitine reveals potential key genes associated with oxidative defense and the semen quality factor in aging roosters

l-carnitine (LC) has been widely studied as a natural antioxidant molecule for treating low-fertility gametes. However, the molecular mechanism of the effect of LC supplementation on the testes of aging cocks has not been evaluated. Therefore, the objective of this study was to reveal the mechanism of testicular oxidative defense induced by LC supplementation in relation to the semen quality factor (SQF) in the aging cock through a transcriptome study conducted from a new perspective. A total of 24 Jinghong cocks at 55 wk of age were randomly divided into 2 groups (n = 12). All cocks were fed a standard isocaloric and isonitrogenic breeder diet (control, LC-0), and the other group was supplemented with LC at 150 mg/kg/d (treated, LC-150) for 12 wk. Remarkably, seminal characteristics and enzymes, sex hormones, and cock fertility related to testicular oxidative defense were considerably improved by LC supplementation. LC-150 testes showed the differential upregulation and downregulation of 97 and 90 transcripts, respectively, compared with LC-0 testes. Most upregulated transcripts were involved in testicular oxidative defense and spermiogenesis optimization, whereas the downregulated genes were responsible for oxidative stress, in contrast to the SQF. Functional analysis of the transcriptionally altered genes indicated the testicular deregulation of long-chain fatty acid and lipid peroxidation, enhancing fatty acid breakdown to release ATP production via β-oxidation. These findings could lay the foundation for the discovery of new molecular markers of SQF-associated LC supplementation and potential targets for therapeutic intervention to optimize fertility in aging cocks.

Effect of dietary supplementation of omega-3 rich oils on seminal characteristics, oxidative stress and plasma testosterone levels in Beetal bucks

The objective of the present study was to evaluate the efficacy of dietary supplementation of omega-3 rich oils, chia seed oil (CSO; Salvia hispanica) and linseed oil (LSO; Linum usitatissimum), on seminal characteristics, and plasma testosterone levels in Beetal bucks. The bucks were fed diet supplemented with either LSO or CSO @1% on dry matter basis or un- supplemented diet for 100 days. Daily intake of omega-3 fatty acids was 0.60 (control diet), 4.04 (diet supplemented with LSO) and 4.57 g (diet supplemented with CSO). Ultrasonography revealed no significant improvement in the testicular size yet the growth rate was much high in animals fed omega-3 supplemented diet. Ejaculate volume, individual motility, viability, sperm concentration, plasma membrane and acrosome integrity were significantly higher in bucks fed CSO supplemented diet as compared to LSO and control diet. The percentage of abnormal spermatozoa was significantly less in bucks fed CSO and LSO diet than control. Melanoaldehyde (MDA, μmole/109 sperm) concentration was lowest in bucks fed CSO diet followed by LSO and control diet. Activity of superoxide dismutase, glutathione reductase and catalase was significantly higher in spermatozoa of bucks fed CSO and LSO diet as compared to control. Bucks fed CSO and LSO supplemented diet had nonsignificantly higher plasma testosterone concentrations than control. These findings indicated that CSO supplementation in the diets may be helpful in improving the process of spermatogenesis, sperm output, quality and fertility of bucks.

Sperm evaluation and morphological description of male genitalia of meerkats (Suricata suricatta)

In ex situ conditions, little is known about the reproductive biology of meerkats. The aim of present study was to describe the morphological aspects of male genital organs and accessory glands using macroscopic evaluation, ultrasonography, and radiography, as well as describing semen characteristics post-electroejaculation. The results indicated anatomical characteristics of meerkats are very similar to those of cats, having a prostate, accessory bulbourethral glands, and an elongated and radiopaque structure in the penis, which is indicative of there being a baculum. The testicular volume was 0.81 cm³ (± 0.10) and the relative testis weight was 1.37 cm³/kg (± 0.15). Both testicles are present in the scrotum, which has an ellipsoidal shape, homogeneous texture, hypoechoic parenchyma and are encased in a hyperechoic tunica albuginea. Electroejaculation was effectively induced in all animals for semen collection with utilization of medetomidine and ketamine. The values semen samples variables were as follows for volume - 0.125 ± 0.193 mL, motility - 19.8 ± 18.6 %, vigor - 1.9 ± 1.0, concentration - 40.5 ± 25.2 × 106 sperm/mL and morphologically normal sperm - 10.8 ± 6.6 %. This is the first study in which there is a description of morphological and imaging aspects of the male reproductive tracts of meerkats, as well as the seminal characteristics after using electroejaculation for semen collection. Knowledge of anatomical and seminal characteristics is essential for implementation of assisted reproduction programs, as well as reproductive management in the species.

Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864).

The seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 µl of diluted semen was added to 10 µl of distilled water to achieve gamete activation. The average duration of total motility was 76.67 s; while the average sperm motility rate at intervals of 15 s was 95.3, 85.3, 59.6, 31.7, 13.0, 4.6 and 1.2%. To determine sperm concentration in samples, 0.5 μl of semen was diluted with 500 μl of glutaraldehyde. An aliquot of 10 μl of this dilution was utilized for cell counting. An average count of 4.97 × 109 ± 3.46 sperm/ml was obtained. Morphological analyses were performed using eosin-nigrosine dye; 20.33% of the sperm were observed to be dead. Live sperm, comprising the other 79.67%, had an average length of approximately 30 µm, with a head diameter of 4.488 ± 0.7 µm; and a flagella plus mid-piece length of 26.071 ± 12.4 µm. Of those sperm, 69% had a normal morphology, while 31% had primary and secondary abnormalities. The observed abnormality rate did not have a detrimental effect on artificial fertilization potential for the species. The description of the seminal characteristics of a species is one of the most important sets of information required for artificial reproduction of fish in captivity. It also contributes significantly to the total biological knowledge of the studied species.

Applications of Organoids for Cancer Biology and Precision Medicine.

Organoid technologies enable the creation of in vitro physiologic systems that model tissues of origin more accurately than classical culture approaches. Seminal characteristics, including three-dimensional structure and recapitulation of self-renewal, differentiation, and disease pathology, render organoids eminently suited as hybrids that combine the experimental tractability of traditional 2D cell lines with cellular attributes of in vivo model systems. Here, we describe recent advances in this rapidly evolving field and their applications in cancer biology, clinical translation and precision medicine.

Administration of equine Chorionic Gonadotrophin (eCG) to rams to improve the reproductive performance during the non-breeding season

In most ram breeds, there are annual changes in testosterone serum concentration and semen quality. This study aimed to determine if eCG administration to rams during the non-breeding season improves the reproductive activity. In the first study, we compared the effects of administering three doses of eCG (0, 400 or 700 IU). In the second experiment we compared the effects of administering eCG to rams from two breeds originated in different latitudes, and thus, with different expected reproductive seasonal pattern (Highlander; HL and Texel; TEX). The first study was performed during the mid non-breeding season with three groups of rams: treated with 400 IU (group eCG400) or 700 IU of eCG (group eCG700), or untreated controls (group CON). Treated rams received three doses of eCG administered every 6 days. Testosterone concentration, testicular traits (scrotal circumference, testicular and sexual skin flush, and ultrasonographic testes pixel color intensity) and seminal characteristics were determined from five days before the first eCG administration until Day 24. Testosterone concentration was greater in eCG700 than in eCG400 (P <0.001) and CON (P <0.0001) rams, and in eCG400 than in CON rams (P = 0.0002). However, the treatment did not modify the testicular traits and seminal quality. The second study was performed with 15 HL and 17 TEX rams; while 8 HL and 9 TEX rams received two doses of 1000 IU of eCG (Days 0 and 5), the other rams remained as untreated controls. Scrotal circumferences, seminal traits (fresh and thawed semen, and cryopreservation ratio –CR-) as well as the sexual behavior were determined, twice before eCG administration and during 3 weeks. The treatment did not modify the scrotal circumference nor the fresh sperm traits evaluated. However, the CR of motile and progressive motile sperm were greater in eCG treated rams than in control rams on Day 7 (P = 0.01 for both). Treated rams had their second and third ejaculations earlier than control rams (P = 0.001 and P = 0.02; respectively). In conclusion, the administration of eCG during the non-breeding season stimulated testosterone secretion without modifying the main reproductive outcomes of rams. Reducing the interejaculatory intervals could be considered for intensive semen collection programs.

Effect of thermal stress on basic seminal characteristics of Gyr bulls.

The objective of this study was to evaluate the influence of thermal stress indices and minimum temperature on the semen quality of dairy Gyr bulls and to determine whether the minimum night temperature is able to balance the thermal stress that occurred during the day. A total of 922 semen samples were collected from 391 bulls aged between 15 and 49months, which participated in progeny pretest trials, from December 2011 to April 2017. Semen characteristics (ejaculate volume, gross motility, sperm motility, sperm vigor, sperm concentration, and percentage of total defects) and scrotal circumference were analyzed. Meteorological data were recorded daily by the National Institute of Meteorology. The analyses were carried out using mixed models. The following fixed effects were included in the model: test, month of sample collection, scrotal circumference, the age of the bull at sampling as covariate (linear regression), and the climatic variables (equivalent temperature index [ETI], temperature and humidity index [THI], or minimum temperature). The effect of the bull nested within the test was included in the model as a random effect. Two periods were considered to evaluate the effect of the climatic variables on sperm quality: 7days before collection (period 1) and the day of collection (period 2). There was an effect of ETI on sperm vigor and concentration in both periods and on volume and percentage of total defects only in the second period. The THI exerted a significant effect on volume in both periods and on sperm motility and concentration only in the second period. There was an effect of the minimum temperature on ejaculate volume, sperm motility, sperm concentration, and percentage of total defects only in the second period, which corresponds to the day of semen collection. Taken together, the results show that there was sufficient thermal stress to negatively affect semen quality. However, the minimum temperature during the night was a significant factor that balanced these negative effects of THI and ETI on semen traits of Gyr bulls.

The European Academy of Andrology (EAA) ultrasound study on healthy, fertile men: clinical, seminal and biochemical characteristics.

Infertility affects 7%-12% of men, and its etiology is unknown in half of cases. To fill this gap, use of the male genital tract color-Doppler ultrasound (MGT-CDUS) has progressively expanded. However, MGT-CDUS still suffers from lack of standardization. Hence, the European Academy of Andrology (EAA) has promoted a multicenter study ("EAA ultrasound study") to assess MGT-CDUS characteristics of healthy, fertile men to obtain normative parameters. To report (a) the development and methodology of the "EAA ultrasound study," (b) the clinical characteristics of the cohort of healthy, fertile men, and (c) the correlations of both fertility history and seminal features with clinical parameters. A cohort of 248 healthy, fertile men (35.3±5.9years) was studied. All subjects were asked to undergo, within the same day, clinical, biochemical, and seminal evaluation and MGT-CDUS before and after ejaculation. The clinical, seminal, and biochemical characteristics of the cohort have been reported here. The seminal characteristics were consistent with those reported by the WHO (2010) for the 50th and 5th centiles for fertile men. Normozoospermia was observed in 79.6% of men, while normal sperm vitality was present in almost the entire sample. Time to pregnancy (TTP) was 3.0[1.0-6.0] months. TTP was negatively correlated with sperm vitality (Adj.r =-.310, P=.011), but not with other seminal, clinical, or biochemical parameters. Sperm vitality and normal morphology were positively associated with fT3 and fT4 levels, respectively (Adj.r=.244, P<.05 and Adj.r=.232, P=.002). Sperm concentration and total count were negatively associated with FSH levels and positively, along with progressive motility, with mean testis volume (TV). Mean TV was 20.4±4.0mL, and the lower reference values for right and left testes were 15.0 and 14.0mL. Mean TV was negatively associated with gonadotropin levels and pulse pressure. Varicocoele was found in 33% of men. The cohort studied confirms the WHO data for all semen parameters and represents a reference with which to assess MGT-CDUS normative parameters.

Semen Parameters of Fertile Guinea Pigs (Cavia porcellus) Collected by Transrectal Electroejaculation.

Simple SummaryThe guinea pig is an important livestock species in some South American countries. Peruvian guinea pig exports are still increasing since 2000. Peru has made genetic improvements on the species since 1986. However, there are few advances in reproductive biotechnology. Thus, there is a lack of information about harmless, efficient semen collection techniques in the species. Consequently, the selection of fertile males takes a long time due to the absence of validated seminal parameter standards. For that reason, it is necessary to set semen reference values through an objective electroejaculation technique for guinea pigs. This study describes semen parameters of fertile males and validates a novel semen collection technique for the species. These values will serve as a reference to detect infertile males and to select the best males for breeding purposes, improving the reproductive performance on farms.The guinea pig, as a livestock species, is still developing and growing throughout Peru and neighboring countries, as reflected by its increasing export since 2000. However, the selection of proven fertile males is tedious due to the absence of seminal parameter standards and the lack of safe semen collection techniques. Thus, pregnancy detection or live births are required for males’ selection. The purpose of this study was to describe the qualitative and quantitative semen parameters of fertile guinea pig males, to set reference values, and to validate a novel electroejaculation technique for the species. Semen was collected at weekly intervals from sixteen fertile males. Four transrectal electroejaculations were performed per male with 95% successful collections, yielding 39 viable semen samples. Seminal characteristics were as follows: pH 7.0 ± 0.13; ejaculate volume 0.67 ± 0.55 mL; sperm motility 90.81 ± 6.64%; sperm concentration 36.7 ± 28.41 × 106 sperm/mL; sperm count 20.09 ± 17.56 × 106 sperm/ejaculate; percentage of abnormal morphology 18.26 ± 8.52%; and percentage ubiquitinated spermatozoa 5.57 ± 6.28%. These values will serve as a reference to detect best breeding and infertile males rapidly. The described techniques are reproducible by commercial producers.

Asynchronous breeding in red brocket deer (Mazama americana): seasonal changes in male reproductive characteristics, seminal parameters, androgen levels, and antler cycle

The red brocket deer is the largest species in the genus Mazama and one of the most abundant and widely distributed cervid in the Neotropics. Yet it has been classified as data deficient by the IUCN, and the limited knowledge on its reproductive biology indicates that red brocket bucks do not possess an annual antler cycle and are capable of breeding during antler casting and growth. Here, in parallel to antler cycle, we investigated seasonal changes in morphometric (body weight, neck and chest girth, and testicular volume), endocrine [plasma testosterone and fecal androgen metabolites (FAM) levels] and seminal (total sperm count and sperm motility index) parameters from captive adult red brocket bucks collected on a quarterly basis over a 1-year period. Two out of six males kept hard antlers year-round, three cast antlers from winter to spring, and one carried velvet antlers for longer than 6 months. No clear seasonal patterns of variation in gross morphometry, seminal traits, and hormonal levels were found, and mean values of all collected parameters did not show differences among seasons. Body weight was positively correlated with most morphometric measurements and seminal parameters, while chest girth was positively correlated with neck girth, testicular volume, and total sperm count. Neither androgen levels (both plasma testosterone and FAM) nor hard antler phase correlated with seminal characteristics. Our findings support that red brocket bucks not only exhibit aseasonal and asynchronous antler cycles, but also maintain their secondary sexual characteristics and semen quality unchanged over the year. This apparent lack of photoperiodic stimuli for controlling reproduction along with an absence of relationship between seminal parameters and antler status might make red brockets unique in terms of reproductive biology among deer species.

Fresh and Post-Thaw Seminal Attributes of Jafarabadi Buffalo Bulls

The study was conducted on four Jafarabadi breeding bulls, 5-6 years old to know the fresh and post-thawed seminal characteristics based on total of 192 semen ejaculates evaluated and cryopreserved over one year period. The mean values of fresh neat seminal characteristics of Jafarabadi bulls, viz., ejaculate volume (ml), colour/density (score), sperm concentration (million/ml), mass activity (score), initial motility (%), live sperm (%), abnormal sperm (%), HOS reactive sperm (%) and acrosomal integrity (%) were 5.19±0.18, 2.38±0.10, 1253.36±24.75, 3.73±0.05, 80.31±0.05, 86.20±0.64, 5.00±0.40, 85.75±0.43 and 93.56±0.56, respectively, whereas the mean post-thawed sperm characteristics, viz., progressive sperm motility, live sperm (%), abnormal sperm (%), HOS reactive sperm (%), acrosomal integrity (%) and first insemination conception rate (%) observed were 57.60±0.36, 66.34±0.53, 8.85±0.33, 56.97±0.46, 75.26±0.17 and 44.63±0.14, respectively. The semen quality of fresh and post-thawed samples observed was within normal limit for use in breeding program with satisfactory first insemination conception rate.

Rats ejaculate prematurely and increase the sperm output during competitive mating

Rats are gregarious and promiscuous and when a female enters the behavioral estrous, two or more males copulate alternately (mount, intromission or ejaculation are performed by any of them). This circumstance promotes male-male competition that in turns influences the copulatory behavior. Thus, olfactory and visual cues of rivals, in laboratory conditions, shorten the ejaculation latency. Other studies focusing in the presence of a rival found the increment in the sperm number. Until now it is unknown if the simultaneous presence of two males simultaneously influences the copulatory performance and seminal characteristics. Thus, the aim of the present work was to evaluate if sexual competition modifies the copulatory behavior, the sperm number and seminal plug. Sexually experienced Wistar rats were tested in two different copulatory contexts. Non-competitive mating (one male-one female), and competitive mating (two males-one female). The copulatory (mounts, intromissions and ejaculation) and seminal parameters (sperm count and copulatory plug) were analyzed. We found that under competition, there was a shortening of the ejaculation latency due to the reduction in the inter-intromission interval and in the number of intromissions. Respect to the ejaculate, the sperm number increased drastically but the seminal plug maintain its size and weight characteristics. Therefore, sexual competition in male rats affects copulatory behavior and sperm count but not the seminal plug.

Amelioration effects of n-3, n-6 sources of fatty acids and rosemary leaves powder on the semen parameters, reproductive hormones, and fatty acid analysis of sperm in aged Ross broiler breeder roosters

This study was conducted to evaluate the effects of dietary polyunsaturated fatty acids (PUFA) sources and rosemary leaves powder (RLP) on the semen quality, fatty acid analysis, and some reproductive hormones of senescent broiler breeder roosters. Thirty-five 45-wk-old Ross breeder roosters were randomly divided into 7 groups (5 birds/group), and received following treatments including control group (basal diet), fish oil (2%), corn oil (2%), an equal (50:50%) proportion of fish oil and corn oil (50:50%), fish oil (2%) with 5 g/kg capsulated RLP, corn oil (2%) with 5 g/kg capsulated RLP, and an equal (50:50) proportion of fish oil and corn oil (50:50%) with 5 g/kg capsulated RLP of diet for 60 D, during which time their seminal characteristics were evaluated every 20 D. At the end of the trial (on day 60), semen samples were tested for determination of sperm fatty acid analysis, lipid peroxidation, and some reproductive hormones. Results showed that feeding fish oil and fish/corn oil with RLP was associated with an increase in docosahexaenoic acid (C22:6n-3) and docosatetraenoic acid (C22:4n-6) in sperm. The fish oil diet increased the proportion of n-3 fatty acids in sperm, and as a consequence, the (n-6)/(n-3) ratio also decreased (P < 0.05). RLP (5 g/kg) to the fish and fish/corn-oil (50:50%)-based diet resulted in improvement in sperm concentration, total motility (%), sperm progressive motility (%), membrane integrity, and viability in terms 0 to 60 day trial (P < 0.05). Diets and age interacted to positively affect sperm concentration and sperm membrane integrity. Also this herbal antioxidant decreased the seminal content of malondialdehyde (MDA) significantly (P < 0.05). Testosterone and LH serum levels of reproductive hormones were significantly higher in fish and fish/corn-oil with RPL (50:50%)-based diet than other groups (P < 0.05). It can be concluded that RLP as an antioxidant could remarkably improve the effects of n-3 and n-3/n-6 PUFA on sperm characteristics, seminal MDA, and hormones levels in aged breeder roosters. The susceptibility of semen to lipid peroxidation was increased in chickens fed without RLP. Future studies are needed to disclose the causal mechanisms involved.

Scrotal thermography to assess the seminal quality in Nelore and Girolando bulls

Bull selection by andrological examination aims to estimate the reproductive capacity of the male. Bulls of zebu origin adapt better to high temperatures than bulls of taurine origin, which may influence scrotum temperature and seminal quality due to the imbalance of testicular thermoregulation. The objective of this study was to investigate the relationship between bioclimatic variables, the temperature of body and scrotal areas, assessed with infrared thermography, and the quality of fresh and post-thawed semen in zebu Nelore bulls (Bos taurus indicus) and Girolando bulls (Bos taurus taurus x Bos taurus indicus). Bulls were kept in pickets with access to water, mineral mix and a diet supplemented with concentrate. Infrared thermographs of the scrotum, orbital globe and muzzle were performed twice a week with a Flir E40 thermal imager. For scrotal thermograms, we analyzed the temperatures of the spermatic cord, proximal and distal portion of the testes and tail of the epididymis using the Flir Tools software. Samples were collected using an artificial vagina and the ejaculates were processed and frozen in liquid nitrogen until further analyses. Data were analyzed with the Tukey test or the Kruskall-Wallis test, depending on their normal distribution. Our results showed differences (p &lt; 0.05) between the two breeds regarding the temperature in the ocular globe, spermatic cord and proximal portion of the scrotum. Nelore bulls presented lower temperature in the body and in certain regions of the scrotum compared to Girolando bulls. Seminal characteristics varied between breeds, with the Nelore breed presenting better semen. Positive correlations were observed between minor sperm defects and ventral regions of the testes and tails of the epididymis in Girolando bulls. Nelore bulls were less influenced by climatic variables and presented lower temperature in skin surface areas in the infrared thermography examination compared to Girolando bulls. Nelore bulls presented superior semen quality in both fresh and thawed samples than Girolando bulls.

Climatic adaptation of bovine subspecies monitored by seminal characteristics during winter months in tropical region

Climatic adaptation of bovine subspecies monitored by seminal characteristics during winter months in tropical region

Reproductive Physiology of Male Rabbits: A Key Factor in Buck Selection for Breeding (Paper Review)

Rabbits are unique animals found in many different continents and climatic zones. Domestic rabbits are the descendants of Oryctolagus cuniculus, a species native to the western Mediterranean basin (Spain and North Africa) and it is said to have originated from the European wild rabbit. Rabbits have been used as experimental animals in genetics and breeding since the beginning of the century. Rabbit bucks are ready for reproduction at 32 weeks of age when sperm production is known to have stabilized. Lack of detailed information about the anatomy and morphological structures of rabbit bucks, their reproductive organs and its physiology, hampers the reproductive index in multiplication and breeding of rabbits. This review is centered on gathering relevant information about the morphology and reproductive physiology of rabbit bucks with emphasis on showing some aspects relating to their sexual maturity, occurrence of puberty, reproductive distinctiveness, seminal characteristics, number, size of glands and their location, sperm production and Spermatogenesis for a better breeding purpose.

Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol

Abstract This study aimed to determine the semen characteristics of Astyanax lacustris after hormonal induction and to evaluate the sensitivity of the species sperm to cryoprotective solutions based on the cryoprotectants dimethyl sulfoxide and methyl glycol. Volume, color, sperm concentration, total motility and aspects of sperm movement were analyzed using “Integrated Semen Analysis System”. Three different extenders were tested: A) glucose 5%+egg yolk 10%, B) BTS®5% and C) glucose 5% and two permeable cryoprotectants: dimethyl sulfoxide (Me2SO) and methyl glycol (MTG). Fresh A. lacustris semen presented total motility of 76.6±11.2%, motility duration of 33.0±2.2s, sperm concentration of 7.22±3.2×109sptz/mL and seminal osmolality of 219±0.03mOsm/kg-1. The toxicity test showed the highest total motility values at the MTG15%+A, Me2SO15%+B and Me2SO10%+C dilutions, and the Me2SO10%+C and Me2SO15%+C dilutions presented the highest values for curvilinear velocity, linear velocity and average velocity. The tested protocol was not effective at maintaining the viability of A. lacustris semen after freezing because no motility was observed in any of the dilutions. However, the Comet Assay demonstrated that cryoprotectant solutions were effective in protecting the genetic material of cells, as DNA damage levels were low, with no difference between control and Me2SO10% + A, dilutions MTG10%+C, Me2SO10%+B and Me2SO15%+B.

Case report on effect of Virechana and Chatuhprasritik Basti in Necrozoospermia

Context: The urge for fatherhood can be biologically as strong as the urge for motherhood. Male infertility is directly related to impairment in the quality of semen. Complete asthenozoospermia (loss of motility) and necrozoospermia (nonviable sperms) are potential causes of male infertility. Necrozoospermia or necrospermia accounts for 0.2% to 0.5% of male infertility. In Ayurveda, these could be considered Kshina Shukra, one of the “Shukradushti” (~vitiation of sperm) where Shukra Dhatu (~reproductive tissue) is of poor quality. Methodology: A 36-year-old patient presented with complaints of having no children in the last 7 years of active married life. Based on laboratory examination, his diagnosis was confirmed as necrozoospermia. The study intervention included, Virechana Karma (~therapeutic purgation) as per the classical textbook guidelines, followed by Chatuhprasrtik Basti (~therapeutic enema) for 16 days. Results: At the end of therapy, semen analysis showed marked improvement in sperm count, increase in sperm motility and normal forms. The positive changes in semen analysis after Virechana Karma match the textbook descriptions of the benefits of Virechana Karma for Shukradushti. Conclusion: Classical Virechana followed by Chatuhprasritik Basti may be effective in improving the seminal characteristics in Necrozoospermia. The present findings with no adverse effects indicate the promising scope of traditional medicine in male infertility disorders.

Season Induced Changes in Seminal Characteristics of Sahiwal Breeding Bulls

Season Induced Changes in Seminal Characteristics of Sahiwal Breeding Bulls