The results of our research devoted to efficiency measurements of energy harvesting from tele-transmission systems featuring examples of model autonomous semi-passive RFID identifiers have been presented in this paper. The selected harvester systems were tested in different systems and circuit configurations to determine of energy harvesting conditions. The appropriate laboratory stand was designed and constructed for investigations in accordance with real-world electromagnetic conditions. The obtained results made it possible to determine the dependence of harvester output voltage in selected applications (operation mode, localization related to the energy source) as well as the minimum power threshold required for identifier operations in the environment. The real energy balance was also established, which is highly useful for configuring identifier parameters and optimizing the operation mode to achieve the best energy harvesting from the electromagnetic environment.