Simple SummaryJumping represents the most popular equestrian discipline. However, traditional selection and training strategies for jumping horses have not been validated using instrumented performance analyses to scientifically inform the optimization of athlete selection, training and competitive performance. We aimed to quantify the role of muscle function during the equine jump, its relationship to athletic performance indicators, and how this objective information can inform equestrian selection and training practices. We used three-dimensional kinematic and surface electromyography data to quantify movement and muscle activation, respectively, from horses executing a submaximal jump. Horses were grouped based on their ability to raise the center of mass during the jump suspension—a fundamental, objective measure of jumping performance. Kinematic data were used to objectively measure equestrian-derived preferences for movement traits related to impulsion, engagement and joint articulation. Horses that raised the center of mass highest during jumping displayed muscle activation and movement strategies that indicate a greater ability to rapidly generate hindlimb muscular force during jump take-off. These findings provide objective support for equestrian preferences related to the generation of engagement, impulsion and hindlimb muscle power when selecting and training jumping horses and justify their prioritization as objective performance indicators for the sport of equine jumping.Selection and training practices for jumping horses have not yet been validated using objective performance analyses. This study aimed to quantify the differences and relationships between movement and muscle activation strategies in horses with varying jump technique to identify objective jumping performance indicators. Surface electromyography (sEMG) and three-dimensional kinematic data were collected from horses executing a submaximal jump. Kinematic variables were calculated based on equestrian-derived performance indicators relating to impulsion, engagement and joint articulation. Horses were grouped using an objective performance indicator—center of mass (CM) elevation during jump suspension (ZCM). Between-group differences in kinematic variables and muscle activation timings, calculated from sEMG data, were analyzed using one-way ANOVA. Statistical parametric mapping (SPM) evaluated between-group differences in time and amplitude-normalized sEMG waveforms. Relationships between movement and muscle activation were evaluated using Pearson correlation coefficients. Horses with the greatest ZCM displayed significantly (p < 0.05) shorter gluteal contractions at take-off, which were significantly correlated (p < 0.05) with a faster approach and more rapid hindlimb shortening and CM vertical displacement and velocity, as well as shorter hindlimb stance duration at take-off. Findings provide objective support for prioritizing equestrian-derived performance indicators related to the generation of engagement, impulsion and hindlimb muscle power when selecting or training jumping horses.