The request for assisted reproductive technology (ART) by couples which man is HIV infected, has drastically increased in order to reduce risks of virus transmission to woman and child. The aim of this study was to verify if elimination of viral particles of HIV soropositive men semen impacts on semen quality and in the pregnancy rate following intracytoplasmic sperm injection (ICSI). Retrospective study. This study included 10 HIV seropositive men (HIV+), aging 37.6 ± 5.0 years submitted to ICSI cycles due to serodiscordance. A control group (HIV−) was composed by semen donors (n = 8), aging 32.7 ± 6.8 years. Semen from HIV- group was collected and routine semen analysis was performed according to World Health Organization (WHO), and morphology by Kruger strict criteria before semen cryopreservation. HIV+ patients were submitted to antibiotic therapy (Norfloxacine 400 mg BID for 3 weeks) previously to semen collection, and seminal samples were analyzed as routine, and processed thought current sperm-wash followed by density gradient method. Before cryopreservation, an aliquot were used to detect the presence of HIV-1 thought real time polymerase chain reaction (RT-PCR). Women were submitted to ovarian stimulation and ICSI was performed with thawed and washed sperm samples. For HIV+ samples, ICSI was performed only when no HIV-1 virus was detected. Seminal parameters (concentration, motility, progressive motility and morphology), and pregnancy rate were compared between groups. Sperm concentration in HIV− (52.1 × 106 sperm/mL) had no statistical difference to HIV+ group (59.2 × 106 sperm/mL; P=0.318). The same was observed when the percentile of motile sperm was evaluated (HIV−: 61.7%, HIV+: 61.9%; P=0.117). However, when just progressive motility was compared, it was observed a significant difference between the groups (HIV−: 68%, HIV+: 48%; P=0.022). Morphology differences were not observed among experimental groups (P=0.684) as well as pregnancy rate (P=0.964). After delivery no seroconversion was observed on mother and child. Semen quality of HIV seropositive patients and its treatment before cryopreservation do not affect ICSI's pregnancy rate, and associated to an adequate antiretroviral protocol, semen processing may be a successful toll in providing safe gametes for reproductive management of serodiscordant couples.