Abstract
Objective: There is a lack of consensus in the literature regarding the cut-off values of seminal oxidative stress (OS) despite a strong correlation between OS and male infertility. Since OS in semen is the result of an imbalance between reactive oxygen species (ROS) production and antioxidant strategies, we hypothesized that levels of ROS in neat semen of men undergoing fertility evaluation may reflect OS status in vivo. The objectives of this study were to 1) establish a cut-off value of seminal OS by measurement of ROS levels directly in neat semen in a group of normal healthy donors (controls) and comparing the results with a population of infertile men, and 2) evaluate the characteristics of laboratory variability of ROS measurement in neat semen.
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