Wildlife monitoring has become increasingly crucial for better examination and protection of wildlife. However, existing monitoring devices are limited and less effective due to battery life limitations. This paper proposes a novel vibration energy harvester (VEH) for self-powered applications in wildlife monitoring. An eccentric semicircular rotor was designed and developed to effectively convert the random movements of wild animals into energy. The proposed vibration energy harvester mainly consists of four components: vibration input module, electromagnetic generation module, power management module, and work module. The electromagnetic power generation module converts the kinetic energy of wildlife harvested by the eccentric semicircular rotor into electrical energy. The power management module converts the generated AC power into DC power; the electricity is stored in a lithium battery to provide power for the wildlife monitoring system. Experiments were conducted to record the harvested power from human and animal kinetic energy. The installed VEH on the leg of the tester produced 25.8 mW output power at the speed of 15 km/h, whereas sika deer resulted in 19.66 mW while running. Finally, an estimation analysis was conducted for sika deer, and found that 212.08 J/day can be harvested for the wildlife tag to work for 15 days.