Guizotia Abyssinica Research Articles

Anti-Limnatis nilotica activity and kinetic study of Guizotia abyssinica hull crude extract in stream water body ecosystems of Ethiopia

In this work, crude extracts of Niger seed hull (NSH) were used to conduct anti-leech (Limnatis nilotica) experimental activity. Following the standard method, the phytochemical screening of methanol and chloroform extracts was evaluated. The crude methanol extract contained secondary metabolites as terpenoids, flavonoids, and saponins. According to severity effects ranging from 4+ to 1+ and timing zones from 1 to 720 min, including negative paralysis, the paralyzing time was calculated. From 1+ to 4+ severities, the crude extract of NSH exhibited strong anti-leech action. Methanol and chloroform extracts exhibit anti-leech properties that are comparable to the reference medication levaminsole. The dosage contraction of NSH in all dose levels of the standard reference drug Lavamisole resulted in a considerable death of leeches, as shown by the first-order reaction (χ2 = 62.9, df = 5, p < 0.05). The methanol and chloroform extracts of NSH significantly differed in how many leeches they were able to kill (χ2 = 355, df = 5, p < 0.05 and χ2 = 711.7, df = 5, p < 0.05, respectively). Differential canning calorimetry (DSC) was used to characterize the stability research of NSH crude extracts. Thermogram results revealed that four principal chemicals were valid in the 49.5 °C to 300 °C temperature range. According to a pharmacokinetic model, methanolic-extracted NSH kills the leech parasite more quickly than chloroform-extracted NSH. The current kinetic parameter results imply that methanol extract NSH has better anti-leech activity, and that it is a time-saving and environmentally acceptable organic chemical for future relevance to prevent anti-leech activity, and might be chosen for further research.

Nutritional and nutraceutical variability in neglected niger (Guizotia abyssinica (L.f.) Cass.) accessions from Eastern Ghats of India

The exploitation of neglected niger accessions plays a crucial role in future breeding programs aimed to enhance the quality. Present study evaluated the genetic variation in nutritional and nutraceutical compositions of 30 indigenous niger accessions originating from Eastern Ghats of India, and compare them with three improved varieties of the locality. The proximate compositions (g/100 ​g) showed significant variations, with moisture ranging from 1.36 ​g to 4.95 ​g, ash from 1.06 ​g to 5.45 ​g, fat from 28.53 ​g to 44.11 ​g, protein from 8.84 ​g to 18.18 ​g, carbohydrate from 11.21 ​g to 35.09 ​g, fiber from 16.32 ​g to 26.62 ​g, and energy from 407.32 ​Kcal to 526.84 ​kcal. Niger seeds are rich in phenol 41.10–82.20 ​mg/g, flavonoid 5.32–15.98 ​μg/g, antioxidant 13.59–31.08% across the accessions. Principal component analysis revealed that the first two axis explained 41.8% of the total variation, indicating significant differences among genotypes. The heritability and genetic advance in percent of mean (GAM) ranged from 61.11% to 99.91% and 12.5%–89.1% among the traits across the populations. High heritability and GAM recorded in vitamin C, ash content, antioxidant capacity, flavonoid and phenol suggests that these traits may be important for niger breeding program. Altogether, some indigenous niger accessions such as Mangardora, Ganjeipadar and Kolabnagar had exceptionally rich in fat, protein and energy contents and are nutritionally superior compared to improved variety of the locality. Further, exceptional rich in flavonoid, antioxidant capacity and vitamin C was recorded in Manahar, Badapada and Mahadeiput. These nutrition rich accessions have ample opportunities for future breeding programs aimed to enhance the quality and hold great potential in food industry for making high value functional food.

Biochemical Screening and Fatty Acid Profiling of Niger Seeds (Guizotiaabyssinica) Grown in India

Biochemical Screening and Fatty Acid Profiling of Niger Seeds (Guizotiaabyssinica) Grown in India

Optimization of process variables to prepare activated carbon from Noug (Guizotia abyssinica cass.) stalk using response surface methodology

Even though adsorption is considered the simple, effective, and efficient method for the treatment of wastewater, accessibility of low-cost and locally available activated carbon remains the challenge. In response to this, recently significant amounts of agricultural byproducts have been investigated to prepare low-cost porous carbon, but there is still a problem related to cost and availability. So, Noug stalk, chosen because of its abundance and low cost as an agricultural byproduct in Ethiopia, was chemically activated with phosphoric acid to produce a low-cost porous carbon. The production of Noug stalk activated carbon (NSAC) is optimized using response surface methodology. A central composite design was used to investigate the effect of three process parameters, namely carbonization temperature (450–650 °C), activation time (90–150 min), and impregnation ratio (w/w) (1–3), on the BET surface area and yield of porous carbon. The analysis of variance (ANOVA) result shows that all three process parameters showed a significant effect on the surface area of porous carbon, while only carbonization temperature showed a significant effect on the yield of porous carbon. The best conditions for NSAC preparation were a carbonization temperature of 537.50 °C, an activation time of 127 min, and an impregnation ratio of 1.95, resulting in a BET surface area and yield of 473.45 m2 g−1 and 53.78%, respectively. The expected and observed values of the model for the outcome variable were highly comparable. Several analytical techniques, including proximal analysis, Fourier transform infrared spectroscopy, and N2 adsorption-desorption, were used to characterize the NSAC. The results demonstrated that the prepared NSAC has a highly porous structure comparable to porous carbon obtained from other biomass feedstocks. This implies it would be used as a potential low-cost alternative for wastewater treatment using the adsorption process.

Allelopathic potential of root and leaf aqueous extracts of invasive alien plant species, Cryptostegia grandiflora, on germination and seedling growth of Linum usitatissimum and Guizotia abyssinica

C. grandiflora has been termed the worst invasive alien plant species. However, its allelopathic potential has not been studied yet. The goal of this research was to study the allelopathic potential of C. grandiflora on the germination and seedling growth of L. usitatissimum and G. abyssinica. Plant specimens and samples of C. grandiflora were collected from the Minjar-Shenkora district, North Shewa Zone, Ethiopia. An experimental investigation was conducted to check the impact of its leaf and root extracts on the growth and germination parameters of the indicated test plant species. The root and leaf samples were extracted with water and methanol (99.85%). Leaf and root samples of 25 g were steeped separately in 250 ml of each of the specified solvents to make the stock solutions. The experiment was conducted in 9-cm-diameter Petri-dishes and laid out in completely random designs, replicated thrice with five blocks, each block representing a different concentration of extract (25, 50, 75, and 100%) and control. Twenty-five seeds of each test plant were placed in a petri-dish along with five ml of each extract. Differences between treatments were evaluated using one-way-ANOVA and Tukey's Honestly Significant Difference (HSD) Test. Results revealed that the inhibitory effect of water extracts of roots and leaves samples was concentration-dependent. With leaf extracts as opposed to root, the inhibitory effect was more pronounced. Hence, when L. usitatissimum seeds were treated with 100% concentration of water extracts from leaf samples of C. grandiflora germination-percentage, seedlings dry-weight, plumule, and radicle length were reduced by 80, 79.3, 73.6, and 79.4% respectively compared to the control. The above parameters also dropped considerably when G. abyssinica seeds were treated with the indicated concentrations. Conversely, the germination of seeds from both test plant species was entirely inhibited in methanol extracts. From this study, it can be deduced that these invasive plant extracts have herbicidal properties. Further phytochemical and molecular study is recommended to identify the active ingredients of C. grandiflora and determine their characteristics. Besides, the integration of identified allelopathic substances as a tool in the management of invasive species is proposed as an environment-friendly option to lessen the deterioration of ecosystem services.

Research Note: Effects of partial replacement of noug seed (Guizotia abyssinica) cake with maggot meal in dual-purpose chicken

The aim of this trial was to evaluate the effect of partial replacement of noug seed cake (NSC) with housefly maggot meal (HFLM) on the growth performance, complete blood count, carcass traits, and gizzard erosion. A total of 120 twenty-seven-day-old Sasso chickens were divided into 4 groups in a completely randomized design. Four diets were prepared to replace NSC with HFLM at the rate of 0%, 20%, 40%, and 60%, labeled as C, T1, T2, and T3, respectively. During the 28-d trial, the chickens were provided feed and water ad libitum. Average daily feed intake (ADFI), final body weight (FBW), and feed conversion ratio (FCR) were not affected by increasing the dietary level of HFLM (P > 0.05). However, there was a difference (P < 0.05) in body weight gain (BWG) between the control and treatment groups, in which a higher BWG was achieved in T1. In this trial, the crude protein (CP), ether extract (EE), and gross energy (GE) in HFLM were 34.06%, 26.85%, and 3871 (kcal/kg), respectively. Blood indices including white blood cells, granulocytes, red blood cells, hemoglobin, and hematocrit were higher in treatment diets (P < 0.05) than in the control diets. No negative effects (P > 0.05) on carcass characteristics were found between the treated and control groups. Furthermore, no discrepancy (P > 0.05) in liver color scores and mortality rate were observed between the control and treatment diets. Surprisingly, feeding HFLM resulted in higher gizzard erosion scores (P < 0.05) in the group fed 40% HFLM. Overall, replacement of NSC at the rate of 20% HFLM in dual-purpose chicken feed produced higher BWG with no gizzard erosion and mortality.

Determinants of Niger Seed Commercialization in the Jabitehenan District, West Gojjam Zone, Amhara National Regional State, Ethiopia

Ethiopia’s oil seed industry is expanding swiftly to meet rising domestic and foreign demand, assisting the country in generating foreign exchange earnings and income. This study aimed to quantify the level of household commercialization of Niger seed output and analyze the determinants affecting it. This research utilized a three-stage random sampling technique to gather quantitative and qualitative data from primary and secondary sources. The primary data came from sampled households through a semi-structured interview. Moreover, 150 samples were selected randomly. Descriptive statistics and the Tobit censored regression model were utilized to analyze the data. SPSS version 20 and STATA 13 software were also employed. The average level of Niger seed commercialization in the area was 0.71%. Several determinants, including the amount of Niger seed output, market information, frequency of extension service, training access, age, and total livestock unit, influenced the level Niger seed. The regional government and district leaders should strengthen the provision of different training to raise the production, productivity, and level of Niger seed commercialization by enhancing the marketable surplus of Niger seeds.

Mutagenic effect of gamma rays and EMS on agronomical traits of niger (Guizotia abyssinica Cass.)

This study was conducted to augment genetic variability in niger plant (Guizotia abyssinica Cass.) using gamma rays and ethyl methane sulphonate (EMS) Niger (G. abyssinica) seeds of cultivar N-71 were exposed to various doses of gamma rays which ranged from 24 to 28 KR and different concentrations of EMS which ranged from 0.2 to 0.4% and in combination. Morphogenic and agronomic traits in the M1 and M2 generations that analyzed included: percentage of germination and survival, plant height, number of branches, capitulum size, number of ray florets per capitulum, number of disc florets per capitulum, head size, number of capitula per plant, number of seeds per capitulum, seed yield per plant and 1000-seed weight. The results of the present study showed that the three gamma irradiation doses used (24, 26 and 28 KR) had decreasing effect in most of the agronomic and morphological traits evaluated in M1. However, combination treatments like 24 KR+0.2% EMS and 26KR+0.4% EMS showed a noteworthy increase of grain yield and yield related components. In general, for the three irradiation doses in M1 population, there were no significant changes observed in morphological traits, however, in progenies of collective mutagenic treated seeds some potential high-yielding mutants were identified.

Responses of Niger [Guizotia abyssinica (L.f.) Cass.] to cadmium and nickel stress

Responses of Niger [Guizotia abyssinica (L.f.) Cass.] to cadmium and nickel stress

Advance Phytochemical Screening of Active Phytocontents of Linum Usitatissimum and Guizotia Abyssinica Plant Seeds in Spectrometry a Study of Comparative Properties

Advance Phytochemical Screening of Active Phytocontents of Linum Usitatissimum and Guizotia Abyssinica Plant Seeds in Spectrometry a Study of Comparative Properties

Antidiabetic potential of Guizotia Abyssinica cass seed oil in Wistar Albino rats - An experimental approach

Guizotia abyssinica Cass. widely grown plant of southern India, seed oil of which are said to be healthy source of oil. Diabetes mellitus is a metabolic disorder affecting major population worldwide. Nutritive healthy source of oils and fats are essential in current scenario. Hence antidiabetic property of Guizotia abyssinica Cass seed oil is tested in Streptozotocin induced diabetes in Wistar albino Rats. Materials and methods: Test drug collected, authenticated, seed oil prepared. Study was conducted in Wistar albino Rats inducing diabetes with Intra peritoneal injection of Streptozotocin. The animals under different test groups received Glibenclamide as standard drug; single and Double dose of G. abyssinica Cass seed oil as test drug and the effect was observed by analysing serum glucose, lipid profile, weight of the Liver and histopathological changes in liver and pancreas after 21 days of experiment. Results: Test drug disclosed decrease in the Serum Sugar level. It has also shown decrease in total cholesterol, LDL and not showed the significant increase in HDL. Histopathological observation of Liver and pancreas proved safety aspect of the drug by preventing the cytotoxic effect of STZ.

Association of Golovinomyces ambrosiae and Podosphaera xanthii in causing powdery mildew disease of Guizotia abyssinica L.f. Cass in India

Association of Golovinomyces ambrosiae and Podosphaera xanthii in causing powdery mildew disease of Guizotia abyssinica L.f. Cass in India

Supplementary effects of Noug Seed (Guizotia abyssinica) Cake with Sesbania (Sesbania sesban) Leaves on Feed Intake, Digestibility and Enteric Methane Emission in Arsi-Bale Sheep

An experiment was conducted to study the effects of feeding the mixture S. sesban (Sesbania sesban) leaves, noug seed cake, and hay on feed intake, digestibility, and estimating enteric methane emission of Arsi-Bale sheep. The experiment was done having the nine-adult sheep of initial body weight 26.7 (±0. 14) kg. The experiment consisted of 7 days of digestibility and 90 days of feeding trials. The experiment employed a 3 x 3 cross over design with three treatments and three periods. There were three treatments including S. sesban leaves alone (T1), 27.6% S. sesban leaves + 27.6% hay +44.8% Noug seed cake (NSC) (T2) and 38.6% + 61.4% NSC (T3). Total dry matter intake (DMI) was higher (P&lt;0.001) for sheep feed in 27.6% + 27.6% hay + 44.8% NSC (706.9g/day) compared to the rest of the treatments. However, the effect of S. sesban leaves alone decrease the total DMI. Digestibility of DM, OM, CP (P&lt;0.01), NDF, and ADF were higher (P&lt;0.05) between the treatments. Average daily gain (ADG) was higher (P&lt;0.01) for sheep in feed 27.6% S.sesban leaves + 27.6% hay + 44.8% than those in feed S. sesban leaves alone. Increased level of S. sesban leaves supplement, in general, reduced growth in this study. However, there was no difference between 27.6% S.sesban leaves + 27.6% hay + 44.8% and 38.6% hay + 61.4% NSC. Estimation of enteric methane emissions factor and daily methane production was higher (P&lt;0.01) in treatments T2 as compared to T3 and T1. And also, T3 higher than T1. Thus, it can be concluded that S. sesban leaves can be promoted as valuable feed resources for ruminants while concurrently reducing methane emissions.

New floristic records from Central Europe 11 (reports 149-163)

The presented 11th part of the series includes 15 new records of vascular plants from Hungary, Slovakia and Ukrainian Carpathians. In Hungary, second country record of Rapistrum rugosum, Amaranthus emarginatus subsp. emarginatus and Pyrus betulifolia were reported. In Slovakia, new localities of Lathyrus hirsutus and seven alien Euphorbia maculata, Nigella damascena, Geranium macrorrhizum, G. purpureum, Pseudofumaria lutea, Ruta graveolens and Salvia sclarea were recorded. Guizotia abyssinica and Lupinus angustifolius are new aliens for the flora of Slovakia. From the Ukrainian Carpathians new findings of Arabidopsis neglecta and Scorzoneroides pseudotaraxaci were recorded. URL: https://www.upjs.sk/pracoviska/botanicka-zahrada/odborne-aktivity/contents-abstracts/

Adoption of recommended cultivation practices in Niger crop (Guizotia abyssinica (L.f.) Cass.) by tribal farmers in Agency area of Alluri Seetharama Raju district, Andhra Pradesh, India

Niger crop is being cultivated in agency areas of ASR district, Andhra Pradesh for millennia with 4000 ha area during the year 2020-2021. The package of practices for cultivation of Niger crop is validated and proven the best as per ANGRAU recommendations. The present study reveals the information on major adopted recommended cultivation practices of Niger crops besides constraints faced by the tribal farmers. A set of 15 recommended practices were prepared as a schedule for personal interview to 60 randomly selected tribal farmers in agency areas and their responses were recorded. The results inferred that among all the schedules, majority farmers had adopted the recommended practices like sowing in time, harvesting within time, application of FYM and drying the harvested produce before bagging with 96.67, 96.67, 95.00 and 93.33 per cent responses. The major constraints include cuscuta weed management, non availability of Niger seed/improved varieties of Niger crop and difficulty in sowing due to dreadful weather conditions like continuous rains. Development of contingency crop plans or suggestive alternate crops are needed during the weather disasters. Through training programmes, method and result demonstrations information on weed, pest and disease management in Niger crop are to be disseminated to the tribal farmers. Village level seed multiplication/production technologies are to be popularised among tribal farmers to encompass the sufficient seed material with vigour and viability to sustain the Niger crop cultivation in agency areas of ASR district, Andhra Pradesh.

Weed control under increasing cover crop diversity in tropical summer and winter

Description of the subject. Weed pressure is a main biotic constraint in tropical agriculture. Cover crop mixtures have increased in popularity to limit weed growth through competition for resources, but the relationship between cover crop diversity and weed suppression is still under debate. Objectives. This study aimed to assess the impact of increasing cover crop diversity (one to four species) on weed control during two growing seasons (tropical summer and winter) in Reunion Island. Method. Weed control was expressed regarding ground cover by weeds and weed aboveground dry mass in the mixtures during four months of growth and its response to cover crop traits was tested using structural equation models. Results. While cover crops reduced weed ground cover and dry mass by 60% and 68% on average in summer and winter, respectively, a higher number of cover crop species within a mixture did not increase mean weed control. Nonetheless, weed control was influenced by the mixture composition and improved when including Guizotia abyssinica. Additionally, cover crop traits explaining weed control differed between growing seasons. In summer, weed control was mainly explained by the final cover crop aboveground biomass and leaf area (depletion strategy). In contrast, weed control was mainly explained by the cover crop rate of increase in ground cover (obstruction strategy) in winter. Conclusions. Using traits to characterize cover crop mixture enables us to identify mixtures of species and traits adapted to different growing conditions. Our study suggests that particular attention on species identity rather than diversity should be paid in mixture to improve weed control in tropical conditions.

Pharmacological and Phytochemical Profile of Guizotia Abyssinica Cass – A Review Beyond its Culinary Utility

The plant kingdom has always been the favourite source of medication in all tradition all over the world. Plants are the source of nutrition and medicaments. It is an approved fact that nothing will exist in this universe without any medicinal properties. This contention is more strengthened by the eternal flow of traditional use of plants as medicine. Last few decades have shown notable resurgence of interest in medicinal plants. Guizotia abyssinica Cass. commonly known as Ramtil Niger regionally called as Gurellu native of Ethiopia cultivated in India also is an erect stout branched annual herb grown for its edible oil and seed. Seeds are specially used to prepare in dry chutney condiments amp as accompaniments in southern India. The ethnomedical claim about this culinary ingredient induced a focus explore it in wide angle. Various parts of the plants like leaves whole plant are commonly used in Agro forestry and Industries. The seeds and seed oil being used culinary it has the nutritional potentiality. It has showed the presence of major fatty acids like linoleic acids lupeol etc. which can be taken as source for the healthy lipids. The anti-oxidant anti-inflammatory and anthelmintic activities have been observed while screening.

Genetic Variability Studies for Yield and Its Attributing Traits in Niger (Guizotia abyssinica (L.f.) Cass)

The experiment was conducted during Kharif 2020 and 2021 at Jawaharlal Nehru Krishi Vishwavidyalaya, Jabalpur, Madhya Pradesh, India. A total of 103 accessions including 03 checks were assessed and observations were recorded for ten traits viz., days to flower initiation, days to 50 % flowering, days to maturity, plant height (cm), number of primary branches per plant, number of secondary branches per plant, number of capitula per plant, number of seeds per capitula, 1000 seed weight (g) and seed yield per plant (g). REML analysis discovered significant differences among the 103 accessions for all the traits. It indicates a satisfactory amount of genetic variability among the accessions for yield and yield-attributing traits. Values of phenotypic coefficients of variation were higher than the value of genotypic coefficients of variation in this study. High heritability was noted for all the traits under the experiment. Genetic advance as a percentage of mean recorded high for seed yield per plant followed by number of capitula per plant, number of seeds per capitula, number of primary branches per plant, number of secondary branches per plant, 1000-seed weight whereas, trait days to 50% flowering, days to flower initiation and days to maturity exhibited low magnitude. These results indicate the dominance of the additive gene effect, which will aid in selection in an early segregating generation.

In Vitro Antidiabetic, Antioxidant, and Prebiotic Activities of the Chemical Compounds Isolated from Guizotia abyssinica.

India and Ethiopia employ Guizotia abyssinica (niger plant) as a source of edible vegetable oil. Previous studies have documented the niger plant's antioxidant properties and dietary benefits. Here, G. abyssinica extract was obtained and ten known bioactive components (1-10) were isolated. The antioxidant, antidiabetic, and prebiotic properties of whole extract and isolated components of niger and the plant's ability to cooperate symbiotically with probiotic strains were examined. Compound 10, myricetin-3-O-L-rhamnoside, had the highest antioxidant capacity measured in the 2,2-diphenylpicrylhydrazyl (DPPH, 4629.76 ± 6.02 µmol Trolox equivalent/g compound) and ferric-reducing antioxidant power (FRAP, 2667.62 ± 7.5 mol Trolox equivalent/g compound) assays. The lowest α-amylase and glycogen phosphorylase activities and glucose diffusion were obtained with whole G. abyssinica extracts, whereas compounds 8-10 had moderate inhibitory effects. G. abyssinica extract also induced the highest glucose absorption by yeast cells in the presence of 5 mM of glucose. Moreover, Lactobacillus plantarum and L. rhamnosus incubated with β-sitosterol 3-O-D-glucoside (compound 7) showed the highest prebiotic activity score. The levels of L-(+)-lactic acid isomer in the probiotic strains were the highest in presence of the whole extract and decreased progressively in the presence of flavonoid glycosides (compounds 8-10) and β-sitosterol 3-O-D-glucoside. The enzymatic profile of the probiotic strains was unaffected by the niger extract and compounds 7-10. The findings revealed that the biological activities of G. abyssinica extract are mediated by the compounds 1-10, and it may be considered as a promising plant for the treatment of diabetes mellitus.

Identification of vanillic acid and its new amide derivative from Hyoscyamus niger and their modes of action in controlling tobacco mosaic virus

Plant viruses represented by tobacco mosaic virus (TMV) are hard to control due to the characteristics of wide hosts and difficulties to be inactivated in vitro . The discovery of candidates to damage the virus directly, and elicitors to induce plant resistances are important strategies against TMV. This study aims to identify new candidates from the title plants as biopesticides in controlling TMV. Herein, vanillic acid ( 6 ) and its new amide derivative, nigeroate ( 1 ), and seven known ones were discovered from the seeds of Hyoscyamus niger L. Compound 1 , elucidated by spectral methods, was synthesized by two steps for industrial application in agriculture as a new elicitor. Compared with ningnanmycin, compound 1 appeared higher curative efficacies against TMV with the inhibitory rates of 58.00% and 69.14% at 250 and 500 μg/mL, respectively, while compound 6 displayed higher inactivation and protective efficacies with the inhibitory rates of 47.30–79.71% and 43.28–63.87% at 125–500 μg/mL, respectively. Compound 6 could destroy TMV particles into small fragments dramatically by decreasing the transcription level of TMV-CP gene, while compound 1 just broke the TMV particles slightly, and was no influence on TMV-CP gene. Interestingly, compound 1 induced potent hypersensitive response (HR), and systemic acquired resistance (SAR) activities (53.82% at 500 μg/mL) in tobaccos. The accumulation of H 2 O 2 by activating SOD (superoxide dismutase), CAT (catalase), as well as POD (peroxidase) was the mechanism of compound 1 induced HR. The SAR induced by compound 1 was referred to upregulate SA (salicylic acid) by activating PAL (phenylalanine ammonia-lyase) pathway, ICS (isochorismate synthase) pathway, as well as PR (pathogenesis-related) proteins due to the increasement of PAL enzyme, and PAL , ICS1 , NPR1 , PR1 , and PR2 transcription levels. Thus, vanillic acid ( 6 ) and its new amide derivative ( 1 ) could be developed as antiviral agents and plant immune elicitors for biopesticides in controlling TMV, respectively. • Vanillic acid and its new amide derivative, nigeroate, were identified from Hyoscyamus niger . • Tobacco mosaic virus coat protein is the target for vanillic acid exerting antiviral activity. • Nigeroate was synthesized by two steps for industrial application as an elicitor. • Accumulation of H 2 O 2 and salicylic acid contributed to nigeroate’s systemic resistance. • Defense enzymes, pathogenesis-related, and salicylic acid biosynthesis-related genes manipulated nigeroate’s resistance.