Black Cumin Seeds Research Articles

Determination of chemical composition of local black seeds Nigella sativa L

Chemical analysis for evaluation of Nigella sativa L. (black cumin) seeds showed a composition of Fat 39% ; Protein 28% ; Carbohydrate 21% ; Moisture 6% and Ash 4.5% . It was found that the black seed contains the following mineral element : Magnesium 0.26 gm /100gm seed ; Calcium 0.25 gm /100gm seed and Iron 25 ?g / gm /100gm seed ; zinc 4.51?g /gm /100gm seed and Copper 3.60 ?g /gm /100gm seed. The analysis also showed that mineral element I. e. ; lead ; Cobalt ; Nickel ; Chrom ; Cadmium and Aresenic are not present . It was found that the fat of the black seed contains the following fatty acids : Myristic 2.8%; Palmtic 16.6%; Stearic 0.8 % ; Oleic 13.79% ; Linoleic 64.2% and Arachidic 1.9% .

Nigellothionins from Black Cumin (Nigella sativa L.) Seeds Demonstrate Strong Antifungal and Cytotoxic Activity.

High-cationic biologically active peptides of the thionins family were isolated from black cumin (Nigella sativa L.) seeds. According to their physicochemical characteristics, they were classified as representatives of the class I thionin subfamily. Novel peptides were called “Nigellothionins”, so-called because of their source plant. Thionins are described as components of plant innate immunity to environmental stress factors. Nine nigellothionins were identified in the plant in different amounts. Complete amino acid sequences were determined for three of them, and a high degree of similarity was detected. Three nigellothionins were examined for antifungal properties against collection strains. The dominant peptide, NsW2, was also examined for activity against clinical isolates of fungi. Cytotoxic activity was determined for NsW2. Nigellothionins activity against all collection strains and clinical isolates varied from absence to a value comparable to amphotericin B, which can be explained by the presence of amino acid substitutions in their sequences. Cytotoxic activity in vitro for NsW2 was detected at sub-micromolar concentrations. This has allowed us to propose an alteration of the molecular mechanism of action at different concentrations. The results obtained suggest that nigellothionins are natural compounds that can be used as antimycotic and anti-proliferative agents.

Self-emulsifying drug delivery system of black seed oil with improved hypotriglyceridemic effect and enhanced hepatoprotective function

Abstract Black Seed Oil (BSO), obtained from the seeds of black cumin (Nigella sativa), has antioxidant, anti-inflammatory, and hepatoprotective effects. The present study was aimed to develop a self-emulsifying drug delivery system (SEDDS) of BSO, to improve hypotriglyceridemic and hepatoprotective function. SEDDS-BSO formed fine micelles with a mean droplet size of 183 nm and improved the dispersion behavior of BSO in water as evidenced by at least 2.6-fold higher dispersibility compared with BSO. In the CCl4-treated rat model of acute hepatic injury, SEDDS-BSO (10 mg-BSO/kg, p.o.) led to 91.7% and 86.8% reductions of alanine aminotransferase and aspartate aminotransferase, respectively, suggesting that SEDDS-BSO could attenuate hepatotoxicity compared to BSO. In addition, repeated oral administration of SEDDS-BSO (10 mg-BSO/kg, p.o.) for 7 days had a potent hypotriglyceridemic effect on rats with corn-oil-induced hypertriglyceridemia compared to BSO. Therefore, the SEDDS approach might be an efficacious dosage option to enhance the nutraceutical properties of BSO.

Nutritional Intervention for the Treatment and Prevention Against Novel Coronavirus-19

Introduction:: The ongoing pandemic of Severe Acute Respiratory Syndrome coronavirus-2 (SARS COV-2) has jeopardized people’s health and the global economy. The infection caused by these viruses inflicts immunosuppression and an unprecedented range of symptoms leading to mortality. At this stage, there are no countermeasures or medicines to overcome rapid disease proliferation and aberrant immunological response. Objectives:: The study aims to determine different immunomodulatory therapeutics that could be potential agents to alleviate viral and other lethal infections and possibly rejuvenate immunological and tissue repair response against this disease. Methods:: A review of the literature was performed by screening different scientific databases to procure various immunomodulatory therapies for the treatment of SAR COV-2. Results:: A comprehensive literature review indicated that different foods rich in vitamins (A-D), selenium and iron can enhance immunological response against various deleterious infections, whereas different nutritious drinks that include hydrogen-enriched water and green tea alleviate inflammation and elicit wound healing properties. Black cumin seeds and Garlic have a myriad of biological activities due to abundant bioactive phytochemicals that play an important role in the elimination of various bacterial and viral infections. Conclusion:: These foods/supplements are relatively safe to consume and possess high toxicity profile and could be a potential nutritional intervention in order to create adequate immunity within a population to fight against this prevailing infection.

PHARMACEUTICAL SIGNIFICANCE OF NIGELLA SATIVA L., A WONDER HERB

Nigella sativa L. (Family: Ranunculaceae; commonly known as black cumin) is an annual herb. N. sativa is cultivated and distributed all over India, especially in Punjab, Himachal Pradesh, Gangetic plains, Bihar, Bengal, Assam and Maharashtra. Among the traditional remedies, prescriptions of Nigella sativa for the treatment of various diseases are well-known since the prehistoric era. Therapeutic effects of Nigella sativa are thought to be due to nigellone and thymoquinone contents. The reported pharmacological properties include protection against disease and chemical-induced renal and hepatic toxicity, anti-inflammatory, analgesic, antipyretic, anti-microbial and antineoplastic activities. The oil decreases blood pressure, cholesterol, triglycerides, and glucose and increases respiration, hemoglobin and packed cell volume. In the traditional system of medicine, black cumin seeds are effective against cough, bronchitis, asthma, chronic headache, migraine, dizziness, chest congestion, dysmenorrheal, obesity, diabetes, paralysis, hemiplegia, back pain, infection, inflammation, rheumatism, hypertension and gastrointestinal problems such as dyspepsia, flatulence, dysentery, and diarrhea. In this review, we will elaborate about this wonder herb having miraculous effects.

Response of lymphatic tissues to natural feed additives, curcumin (Curcuma longa) and black cumin seeds (Nigella sativa), in broilers against Pasteurella multocida

The antibiotic residues and pathogenic resistance against the drug are very common in poultry because of antibiotics used in their feed. It is necessary to use natural feed additives as effective alternatives instead of a synthetic antibiotic. This study aimed to investigate the immune response of Nigella sativa and Curcuma longa in broilers under biological stress against Pasteurella multocida. The total 100, one-day-old chicks were divided into 5 groups. Groups 1 and 2 served as control negative and control positive. Both control groups were receiving simple diet without any natural feed additives, but the infection was given in group 2 at day 28 with the dose of 5.14 × 107 CFU by IV. Groups 3A and 3B were offered 2% seed powder of Nigella sativa, groups 4A and 4B were offered C. longa 1% in powdered form, and group 5A and 5B were offered both C. longa 1% and N. sativa 2% in the feed from day 1 and groups 3B, 4B, and 5B were challenged with P. multocida. The haemagglutination inhibition titter against Newcastle Disease virus (NDV), feed conversion ratio, mortality, gross, and histopathology were studied. The results of this study revealed that hemagglutination inhibition titers against NDV were highly significant (P < 0.05) in treated groups, highest titers (3A, 6.8; 3B, 6.4; and 5A, 7.2) were obtained from treated Groups. The feed conversion ratio of N. sativa + C. longa treated groups (5A, 1.57, and 3A, 1.76) were higher than that of other nontreated groups. The gross and histopathological changes were much severe in control positive, but fewer changes were seen in treated groups. Therefore, we recommend that natural feed additives, black cumin (N. sativa) and turmeric (C. longa), act as an immune enhancer in broilers against P. multocida.

EFFECTIVENESS TEST OF BLACK CUMIN SEEDS (NIGELLA SATIVA) EXTRACT ON THE GROWTH OF KLEBSIELLA PNEUMONIAE AND PSEUDOMONAS AERUGINOSA BACTERIA

Black cumin seeds are known to have beneficial contents as antibacterial. This study aimed to determine the effectiveness of black cumin seed extract (Nigella sativa) on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa. The method used was the disc diffusion method. The ethanol extract of black cumin seeds gain by maceration method and was made into various concentration (5, 7.5, 10, 12.5, and 15)%. For positive control, ciprofloxacin disc were used and negative control used distilled water. The research model used was in vitro experimental research. The results showed that ethanol extract of black cumin seeds have antibacterial activity against Klebsiella pneumoniae with inhibition zone diameters (4.5, 6.7, 10.1, 11, 12.8) mm, Pseudomonas aeruginosa with inhibition zone diameters (7.5, 12, 12.6, 13.4, 15.6) mm, positive controls respectively (24.3 and 20.7) mm and negative controls (0). It can be concluded that black cumin seed extract has an inhibiting effect on the growth of Klebsiella pneumoniae and Pseudomonas aeruginosa.

Physiochemical properties of Saudi Nigella sativa L. (‘Black cumin’) seed oil

The seeds of Nigella sativa L. (Ranunculaceae), commonly known as black cumin seeds and Habat Al-barkah in Saudi Arabia, are used extensively for flavouring and medicinal purposes. This work reported the study of physiochemical properties of Saudi black cumin seed oil (BCSO). The results of hexane extraction showed that black cumin seeds are rich in oil (43.7%). All the oil samples show high saponification values and low unsaponification values. High iodine values (122.7 mg/100 g) showed high degree of unsaturation (86%) for Saudi BCSO with about 60% of dominant linoleic acid (C18:2). The volatile compounds presence in Saudi BCSO were extracted using steam distillation method and analyzed using gas chromatography mass spectrometer (GC/MS). The results showed that the volatile compounds such as p-cymene (31.50 ± 0.51%) and thymoquinone (25.35 ± 0.37%) were dominantly high in Saudi BCSO. Other compounds such as 3,5-dimethyl cyclohexanol, α-thujene, carvacrol, paeonol and longifolene were also present. Vitamin E was analyzed using High performance liquid chromatography (HPLC)-fluorescence method. The total concentrations of vitamin E were 451 ppm. The Saudi BCSO showed high content of linoleic acid (59.8%). The HPLC results showed that major triacylglycerols (TAGs) of Saudi BCSO were 1-oleoyl-2,3-dilinoleoylglycerol (OLL; 37.7 ± 0.4%) and 1,2,3-trilinoleylglycerol (LLL; 35.9 ± 0.3%). On the other hand, minor TAGs present were 1-palmitoyl-2,3-dilinoleylglycerol (PLL; 6.7 ± 0.9%), 1,2,3-trioleylglycerol (OOO; 6.4 ± 0.5%) and 1,2-dioleyl-3-linoleylglycerol (OOL; 5.7 ± 1.1%). The Saudi BCSO exhibited specific physicochemical properties and might be used for medicinal applications.

СОСТАВ И БИОЛОГИЧЕСКИЕ СВОЙСТВА МАСЛА ЧЕРНОГО ТМИНА

Since ancient times, black cumin seeds have been widely used in folk medicine, but thanks to modern scientifi c research and the discovery of a rich phytochemical composition, Nigella sativa can serve as an important basis for the creation of medicines and dietary supplements in classical medicine, as they are a valuable source of polyunsaturated fatty acids and biologically active substances

Inhibitory Roles of Nigella sativa seed extracts on in vitro glycation and aggregation

Background: Glycation is the multi-step process wherein the carbonyl group of glucose reacts with the amino group of other nitrogen-containing compounds like proteins and DNA. This interaction leads to the generation of harmful metabolites commonly known as advanced glycation end products (AGEs). These AGEs have been implicated in various diseases such as cataracts, diabetes, and Alzheimer's. Objectives: The aim of the present study was to check the effect of Nigella sativa seed extracts on glycation and aggregation and for this purpose, two proteins (bovine serum albumin [BSA] and hemoglobin) and two sugars (glucose, and ribose) were selected. Materials and Methods: The amount of glycation mediated products was measured by established methods such as browning, nitroblue tetrazolium (NBT) assay, carbonyl content, and aggregation assays in the presence or absence of extracts (1 mg/ml). The effect of extracts was also checked on glycated DNA by agarose gel electrophoresis. Results: Analysis of results indicates that extracts (1 mg/ml) significantly prevented the generation of early products up to 30.57% (BSA-Rib) and 64.90% (Hb-Glu). The AGEs formation was also suppressed in different glycation systems up to 60.76% (BSA-Glu), 30.79% (BSA-Rib) and 10.74% (Hb-Glu) in the presence of extracts. There was also a decrease in the level of glycation-mediated protein aggregation. Seed extracts were able to prevent the glycoxidative damage of DNA. Conclusion: It can be concluded that black cumin seeds have antiglycating and antiaggregation potential as well as antioxidant properties. Further characterization will help in the identification of active compounds which can be used for the prevention of secondary complications of diabetes and aggregation.

Therapeutic Use Of Vegetable Oils As Functional Food In Alleviation Of Experimental Depression

This article reports the analysis of phenol content, antioxidant capacity of the oil of black cumin, flax, sesame, mustard and fenugreek seeds, and garlic extract. The oils with strong antioxidant activity were tested for alleviation of experiment depression. Folin-Ciocalteu reagent was used to determine the phenol content and DPPH (2,2-diphenyl-1-picrylhydrazyl) was employed for the antioxidant activity

Effects of different levels of dietary black cumin (Nigella sativa L.) and fenugreek (Trigonella foenum-graecum L.) and their combination on productive traits, selected blood constituents, microbiota and immunity of broilers

The effects of inclusion of powdered seeds of black cumin (B) (Nigella sativa L.) and fenugreek (F) (Trigonella foenum-graecum L.) on productive traits, selected blood constituents, microbiota and immunity of broilers were studied. A total of 648 day-old chicks were randomly assigned to nine treatments, with four pen replicates, each with 18 birds, including three levels of B seed powder (BSP; 0, 5 or 10 g/kg) and three levels of F seed powder (FSP; 0, 5 or 10 g/kg) in a 3 × 3 factorial arrangement. Neither powder affected feed intake. The FSP increased (p = 0.048) feed conversion ratio (FCR), but decreased daily BW gain (p = 0.02) between days 0 and 21, while BSP increased daily gain between days 22 and 42 and overall (both p = 0.005). Abdominal fat was decreased (p = 0.003) by BSP. Blood constituents were unaffected by either powder, but ileal Escherichia coli were decreased (p = 0.039) at day 42. The BSP increased a range of immunological titers, where BSP affected positively the measured variables. The interactions between BSP and FSP, specifically on broiler carcass cuts, suggested that where BSP is included at 10 g/kg, the inclusion of FSP at the same level may provide no additional benefit. Thus, while either powder could be included separately, the co-inclusion of both at 10 g/kg is not recommended.

Development of biofuel from Nigella sativa biomass and its suitability for energy application

Nigella sativa is one such plant species indigenous to Middle-east, Eastern Europe, and the Asian Subcontinent. Black cumin seeds derived from N. sativa are one of the identified biomasses to produce biodiesels for the use in an unmodified CI engine. The current study aims to evaluate the credibility of Nigella sativa methyl ester (NSME) as a suitable blend alongside mineral diesel in a common rail direct Injection engine via fundamental combustion analysis. In the present work, 20% of Nigella sativa methyl ester has been tested in diesel engine at 600 bar injection pressure under different pilot and main injection forms. The pilot injection mass has been varied from 5–15% along with variation in main injection also. An optimized injection strategy has been found with respect to performance and emissions for effective implementation of EGR at 10% and 20% rate. At 5% pilot injection, the NSME20 fuel showed comparable brake thermal efficiency and brake-specific fuel consumption at all engine loads. Furthermore, NOx emission of NSME20 fuel has been reduced by increasing the pilot injection rate when compared to diesel and this has been further reduced with 10–20% exhaust gas recirculation. On the other hand, the combustion characteristics of NSME20 blend also shown similar pattern like diesel fuel with slight reduction in cylinder pressure and heat release rate. Finally, it has been concluded that the newly developed Nigella sativa methyl ester can be used as sustainable biofuel for various energy applications in the present scenario.

Growth, Production and Bioactive Content of Several Black Cumin (Nigella sativa L.) Accessions With Different Harvesting Times in a D3 Type Climate Regime

&#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; Black cumin (Nigella sativa L.) is a species Ranunculaceae family which grows in the Middle Eastern and Southern Mediterranean countries. Black cumin is also known as habbatus sauda , or kalonji in South Asia. Black cumin seeds are used as spice traditional medicine; the essential oil compound in the seeds has been identified as thymoquinone. Thymoquinone has a potent antioxidant effect, which could protect organs from oxidative damage by generating free radical agents. Indonesia is one of the most geographically diverse country in the world. Some regions have scattered low and high plains, abundant sunlight, evenly distributed rainfall throughout the year, and highly diverse soil types. The purpose of this study was to study the growth, production, thymoquinone and thymol content of several accessions of black cumin with different harvesting time in D3 type climate regimes. The research was conducted from April to September 2019 in Ngadirejo Village, Sukapura District, Probolinggo Regency, East Java with an altitude of 1.680 m above sea level. The experiment used a randomized complete block design with one factor for the vegetative phase, namely accession (“American”, “Indian”, “Kuwait”, and “Slovenian”) and two factors for the generative phase, namely accession and harvesting time. The harvesting time of black cumin was conducted at 6, 7, and 8 weeks after the anthesis. “Indian” and “Slovenian” accession demonstrated the best vegetative growth, whereas “Indian” had the highest capsules number per plant, seeds weight per plant, and the highest estimated production compared to other accessions (603 kg seeds. ha-1). “American”, “Indian” and “Kuwait” accessions are well adapted in D3 type climate. The harvesting time of 7 to 8 weeks from anthesis was shown to be optimal for all accessions. Thymoquinone and thymol content for the four accessions was circa 349.64– 3,030.45 μg. g-1 of seeds and 385.56 – 2,003.46 μg.g-1 of seeds, with potential production values of 0.18 - 1.83 kg.ha-1 and 0.19 - 0.45 kg.ha-1, respectively. The optimal harvesting time to maximize seed production was unaffected by the black cumin accession.&#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D;

De-Colourisation of Textile Dye Effluents using cost-effective Nigella Sativa Seed Waste

The textile industry is one of the major industries contributing to water pollution. The wet processing of textiles involves the usage of a wide variety of chemicals and dyes. This water-intensive process can potentially affect the water bodies by its effluents. The treatment of dye effluents and reusing of the water could be the possible solution to reduce the impact. Adsorption is one of the most common methods used for textile effluent treatment. Various bio-adsorbents are explored to make the adsorption more sustainable. Nigella Sativa (Black cumin) seeds and its oil are having good medicinal value. The seed-waste left after the oil extraction is found to have active components that can be used as an effective bio-adsorbent. The dye removal efficiency of Nigella Sativa seed-waste is investigated under different experimental conditions (varied adsorbent dose, temperature, pH, and contact time) for reactive dyes. The maximum removal efficiency of 91% is obtained at the optimized experimental condition. Thus the study emphasizes that the no-cost Nigella Sativa seed-waste can be used as an effective bio-adsorbent for reactive dye removal from dye effluents.

Volatile compounds of black cumin (Nigella sativa L.) seeds cultivated in Bangladesh and India

The compositional analysis of volatile compounds of Nigella sativa L. seeds obtained from India and Bangladesh was carried out in this study. Apart from the proportion of volatile compounds, the chemical composition of seeds from both sources were similar. The major volatile compounds in Bangladesh seeds were p-cymene (36.35%), thymoquinone (29.77%), α-thujene (12.40%), carvacrol (2.85%), β-pinene (2.41%), limonene (1.64%), methyl linoleate (1.33%) and sabinene (1.18%), contribution of these is 87.93% of the total volatile oil. On the other hand, the major volatile compounds in Indian seeds were p-cymene (41.80%), α-thujene (13.93%), thymoquinone (10.27%), methyl linoleate (4.02%), carvacrol (3.65%), β-pinene (2.96%), d-limonene (2.11%), 4,5-epoxy-1-isopropyl-4- methyl-1-cyclohexene (1.80%), sabinene (1.50%) and 4-terpineol (1.22%); contribution of these were 83.24% of the total volatile oil. In both seeds, p-cymene, thymoquinone, and α-thujene were the major components. Importantly, N. sativa seeds of Bangladesh contained almost 3-fold thymoquinone compared to Indian seeds. In conclusion, the seeds from Bangladesh contain a higher amount of terpene ketones (29.86%) represented by thymoquinone in comparison to Indian seeds (10.61%); on the other hand, Indian seeds contained a higher amount of terpene hydrocarbons (63.18%) mainly p-cymene, compared to Bangladesh seeds (54.53%). This is the first study to report detailed compositional analysis and comparison of Nigella sativa L. seeds from Bangladesh and India.

Correction: Prophetic Medicine-Nigella Sativa (Black Cumin Seeds) - Potential Herb for COVID-19?

[This corrects the article on p. 62 in vol. 23, PMID: 32685234.].

Uji Potensi Daya Hambat Ekstrak Metanol Biji Jintan Hitam (Nigella Sativa L.) Terhadap Bakteri Streptococcus

One of the plants that are often used as medicinal plants is Black Seed, Black Seed is a medicinal plant known in Indonesia by the name of black cumin. Black cumin seeds and essential oils (Nigella sativa L.) have been widely used in traditional medicine. Many studies have proven the effects of Nigella sativa extract. In various studies, black cumin seed oil shows efficacy as an anti-cancer, anti-free radical and immunomodulator, analgesic, antimicrobial, anti-inflammatory, a spasmolytic, bronchodilator, hepatoprotective, and anti-hypertensive Advanced chemical analysis found that Black Seed contains carotene that is converted into vitamin A by heart. The purpose of this study was to determine the potential inhibition of methanol extract of black cumin seeds (Nigella Sativa L.) against Streptococcus. This research uses a quantitative approach with an experimental method. This research was conducted in the Pharmacognition and Microbiology Laboratory of the Faculty of Health Sciences, Muhammadiyah University, Palangkaraya. The sample used was a sample of plant material, namely Black Cumin Seed (Nigella sativa L) which had been dried and pollinated and then extracted and then tested against bacteria. The results of testing the antibacterial activity showed that from low concentration to high concentration the diameter of the resulting inhibition showed that the activity could inhibit the bacteria streptococcus mutants. The highest inhibition at a concentration of 6% is 0.83 mm and the lowest concentration at a concentration of 3% against Streptococcus mutants.

Reduce graphene oxide-manganese oxide-black cumin based hybrid composite (rGO-MnO2/BC): A novel material for water remediation

Herein, the synthesis, characterization, and water treatment application of reduced graphene oxide-manganese oxide-black cumin hybrid composite, rGO-MnO2/BC, has been reported. The rGO-MnO2/BC was synthesized by incorporating MnO2 particles into reduced graphene oxide functionalized with functional groups of black cumin seeds. The prepared rGO-MnO2/BC was highly amorphous, having large numbers of functional sites, and was evaluated for adsorptive removal of arsenic and methylene blue from their respective aqueous solutions. Adsorption capacities of rGO-MnO2/BC for methylene blue and arsenite were evaluated in batch manner under optimum conditions. 1.0 g/L dosage of rGO-MnO2/BC could remove approximately 99 % of methylene blue and arsenite from initial concentration of 10.0 and 1.0 mg/L, respectively, after 75 min at 7.0 pH, and 27 °C temperature. The sorption data was evaluated to propose the adsorption mechanism by fitting into isotherm and kinetic models. The process of methylene blue and arsenite sorption was proved to be exothermic and feasible based on thermodynamic parameters. The Freundlich isotherm was most suitable for arsenite sorption and both Langmuir and Freundlich isotherms were suitable for methylene blue sorption data. The sorption kinetics followed pseudo-second order relationship for both adsorbates. The exploration of thermodynamics, isotherms, and kinetics parameters revealed physicochemical sorption mechanisms for both adsorbates onto rGO-MnO2/BC surface. The Langmuir sorption capacity of rGO-MnO2/BC for methylene blue and arsenite were found to be 232.5 and 14.7 mg/g, respectively, at 27 °C. The rGO-MnO2/BC is easy to prepare and is low-cost material, and showed high adsorption capacity as compared to the other reported adsorbents. Therefore, the application of rGO-MnO2/BC will be beneficial for upcoming research on water treatment technologies.

The Potential of Black Cumin (Nigella sativa, L.) Seeds Extract to Prevent Polyphagia and Weight Loss in Rattus Norvegicus of Diabetes Mellitus-Type 2

Patients of Diabetes Mellitus-type 2 (DM-2) is not only characterized by an increase of blood glucose levels, but also it is characterized by polyphagia and weight loss. This research aimed to discover the potential of 80% ethanol extract of Indonesia Nigella sativa L. (black cumin) seeds to the feed consumption level and changes in body weight of DM-2 rats model due to the administration of High Fat Diet (HFD) followed by the induction of streptozotocin (STZ) in a dose of 30 mg/kg Body Weight (BW). The DM-2 rats as the induction results divided into five groups, it was given a treatment using N. sativa extract with different doses, i.e. 0, 24, 48 and 72 mg/kg BW (DM-0, DM-24, DM-48, DM-72), positive control used metformin 45 mg/kg BW (DM-Metf). Non-DM rats (Normal) used as reasonable control. HFD induction carried out for 14 weeks, and N. sativa therapy conducted for four weeks after the oral glucose tolerance test. During the treatment, an observation of the feed consumption level and weekly weight gain were carried out. The data obtained were tested using one-way ANOVA, and it continued by Duncan Multiple Rank Test (DMRT), α= 5%. The research results indicated that the administration of 80% ethanol extract Indonesia N. sativa using the doses of 24 mg/kg BW and 48 mg/kg BW can control the feed consumption levels and prevent significant weight loss (p0.05)