Sugarcane Seedlings Research Articles

Phosphorus Quantification in Sugar Cane (Saccharum officinarum) Leaves In Vivo by Portable X-ray Fluorescence Spectroscopy

This study aimed to evaluate the potential of portable X-ray fluorescence (pXRF) equipment to quantify P in sugar cane leaves in vivo. Therefore, sugar cane seedlings were grown in soil fertilized ...

Nutritional and Structural Role of Silicon in Attenuating Aluminum Toxicity in Sugarcane Plants

We investigated the interactive role of Si-mediated attenuation to aluminum (Al) toxicity in two sugarcane cultivars (‘CTC9002’ and ‘CTC9003’) grown in hydroponic conditions. Two pot experiments were distributed in randomized blocks in a factorial design (4 × 2) with four replications. The treatments consisted of 0, 10, 15, and 20 mg L−1 Al (as aluminum sulfate [Al2 (SO4)3·18H2O)], which were combined with the absence and presence of Si [(2.0 mmol L−1 as potassium silicate (K2SiO3)]. Both sugarcane cultivars (‘CTC9002’ and ‘CTC9003’) were affected by Al toxicity (above 10 mg L−1), resulting in nutritional disorders and decreasing plant growth, which were drastically reversed by Si addition in the growth medium. Si supplementation decrease Al concentration and translocation to the shoots of both cultivars when Al and Si were simultaneously supplied in the growth medium. We demonstrated that in shoots of both sugarcane seedlings, Si concentration are positively related to the lignin concentrations (ranging from 12.0% to 41% in cv. ‘CTC9002’ and 12% to 47% in cv. ‘CTC9003’). In addition, Si fertilization enhanced mineral nutrition and use efficiency of macros- and micronutrients, irrespective of the cultivar. Therefore, the use of cultivar ‘CTC9003’ under Si fertilization is more recommended to cope with the adverse effect caused by Al stress. The findings of this study suggest that Si fertilization in sugarcane seedlings is an economic and viable strategy strongly recommended to cope with the adverse effect caused by Al toxicity at concentrations less than 20 mg L−1, which lead to increase the shoot biomass production.

PREDICTIVE MODELS OF CHLOROPHYLL CONTENT IN SUGARCANE SEEDLINGS USING SPECTRAL IMAGES

Chlorophyll content is a widely used parameter for nutritional status diagnosis in sugarcane. This study aimed to develop a predictive model of chlorophyll content in sugarcane seedlings using spectral imagery analysis within the electromagnetic spectrum visible range. The experiment was carried out in a split-plot design, with two fertilization rates and three sugarcane cultivars. For chlorophyll analysis, 144 leaves were collected from seedlings. Chlorophyll contents were extracted and measured by SPAD-502 meter. Spectral images within the range of 480 to 710 nm were analyzed using reflectance, absorbance (white source), and fluorescence (source at 405 and 470 nm) responses. Predictive models were developed using multivariate regression methods such as Principal Component Regression and Partial Least Squares Regression. We chose the best model through absorbance response using variable selection and the PLSR method (R2P = 0.718 and RMSEP = 7.665). The wavelengths of 480, 490, 500, 600, 630, and 640 nm were identified as the best for total chlorophyll content determination. The spectral image processing-based method can provide a chlorophyll measurement equivalent to SPAD, with the advantage of having a higher spatial coverage over the entire leaf area. Besides, it can also support automation of the chlorophyll measurement in greenhouses.

Plant growth promotion of micropropagated sugarcane seedlings var. Co 412 inoculated with endophytic diazotrophic bacteria and effects on the Ratoon Stunting Disease

Plant growth promotion of micropropagated sugarcane seedlings var. Co 412 inoculated with endophytic diazotrophic bacteria and effects on the Ratoon Stunting Disease

Application of Trichoderma on single bud Sugarcane (Saccharum officinarum L) seedlings originated from different stems

The aim of this study was to determine the growth of sugarcane seeds using Trichoderma application and different parts of the stems using the single bud method. The research was conducted at the Experimental Garden at the Faculty of Agriculture, Hasanuddin University, Tamalanrea, Makassar. The research took place from July to November 2018. This research was conducted in the form of a two-factor factorial experiment which was arranged based on a randomized block design. The first factor is Trichoderma with 3 levels of doses, namely without Trichoderma, 15 g/polybag and 30 g/polybag, while the second factor is the stem which consists of the upper stem, middle stem and lower stem which consists of 9 treatment combinations, respectively. -Each treatment was repeated 3 times (3 polybags/treatment) so that the total poly bag used was 81. The results showed that treatment without Trichoderma gave the best results on plant height, namely 13.91 cm at 2 WAP. While the treatment of the middle stem gave the best results on the number of tillers, namely 10.71.

RESPONS BIBIT TEBU (Sacharum officinarum L.) TERHADAP KONSENTRASI ZPT DAN LAMA PENYIMPANAN BAHAN BIBIT

This research was conducted at the STIPER Dharma Wacana Metro Experimental Garden, Rejomulyo Village, Metro Selatan Subdistrict, metro city from October 2020 to December 2020. This study used a complete randomized block design (RAKL) arranged in a factorial with three replications. The first factor is concentration (K) which consists of three levels, namely k1 = 2ml / l, k2 = 7ml / l, and k3 = 10.5ml / l. The second factor was the storage time for the seed material (L), which consisted of three levels of l1 = 3 days, 12 = 5 days, and 13 = 7 days. The results showed that, the concentration of ZPT had no significant effect on the growth of sugarcane seeds except for the percentage of shoot growth, the best ZPT concentration was found at 2 ml / l. The storage time for seed material had no significant effect on the growth of sugarcane seeds. There was no interaction between the concentration of ZPT and the storage time for sugarcane seedlings on the growth of sugarcane seeds.

Pengaruh Dosis Dan Waktu Aplikasi Poc Ampas Kopi Terhadap Pertumbuhan Benih Tebu Bud Set Varietas Cening

This study aims to determine the dose and time of application of the best coffee dregs Liquid Organic Fertilizer (POC) for sugarcane seedlings (Saccharum officinarum L.) cening variety bud set. The sugarcane seeds used are obtained from PG. Lestari, Patianrowo, Nganjuk, East Java. The study was compiled using a randomized block design (RBD) with 2 treatment factors. The first factor is the POC dosage of coffee dregss with treatment variations P0 without fertilizer application, P1 with a dose of 10ml / liter of water, P2 30ml / liter of water, and P3 50ml / liter of water. The second factor is application time with 3 variations of treatment, namely M1 POC applied to sugarcane 1-3-5 weeks after planting (MST), M2 at sugarcane age 2-4-6 MST, and M3 at sugarcane 3-5-7 MST age. Data analysis used variance (ANOVA) with a significant 5%, if there is a significant difference, continue with the Duncan Multiple Range Test (DMRT). The results showed that the interaction between dose treatment and application time of coffee dregs POC had no significant effect on plant height, number of leaves, stem diameter, root length, wet weight and dry weight. Different dosage treatments had a significant effect on plant height, number of leaves, root length, wet weight and dry weight. Different application time treatments had a significant effect on plant height, wet weight and dry weight. The best dosage for coffee dregs POC for sugarcane is 30 ml / liter of water. The best application time for coffee dregs POC is at the age of 3-5-7 MST sugarcane.

Bamboo Shoot Extract as a Natural Plant Regulator Growth (PGR) Able to Increase Sugarcane Seedling (Saccharum officinarum L.) Growth

Sugarcane (Saccharum officinarum L.) is a plant that propagated by vegetative, one of which is the budset system. This propagation is mostly done by farmers, but they have stocks in ordering the roots. One of the efforts to accelerate the formation of these roots can be done by using a Plant Regulator Growth (PGR). PGR from synthetic materials is widely sold in the market, but there are also natural ingredients that can function as PGR, one of which is bamboo shoot extract. This research was to know the effect of bamboo shoot extract concentration as a natural PGR on the growth of sugarcane (Saccharum officinarum L.). The experiments was conducted at the LPP Yogyakarta Polytechnic practical garden, Wedomartani, Sleman, Yogyakarta. This experiment was laid on non factorial Randomized Complete Block Design consisting of 5 treatments which is the concentration of bamboo shoot extract with a ratio of 2:1, 4:1, 6:1, 8:1 (bamboo shoot extract : water), and Control. The results showed that bamboo shoot extract with a concentration of 4:1 is the best treatment. That treatment (4:1) was produce the higher plants, number of leaves, number of tillers, stem diameter, root length, and root wet weight were better than other treatments.

Pengaruh Konsentrasi Zat Pengatur Tumbuh dan Bagian Asal Bibit Terhadap Pertumbuhan Vegetatif Pembibitan Tebu

This study was conducted on Pujodadi Village, Trimurjo, Central Lampung from September to December 2020. The study used a factorial randomized block design with two factors and three replications. The first factor is concentration of plant growth substances which consists of 5 levels; Z0 (0 ml.lt-1), Z1 (3.5 ml.lt-1), Z2 (7 ml.lt-1), Z3 (10.5 ml.lt-1) and Z4 (14 ml.lt-1). The second factor is the origin of the seed consists of three parts; P1 (top), P2 (middle), and P3 (bottom). The research objective was to study the effect of plant growth substances concentration and part of seed origin in sugarcane seedlings. The results showed that the plant growth substances concentration significantly increased the percentage of growth and the time 50% of the seeds sprouted. The seed origin of the top part showed the best growth compared to the middle and bottom. There was no interaction between plant growth substances concentration and seed origin on sugarcane seed growth.

Low absorption of silicon via foliar in comparison to root application has an immediate antioxidant effect in mitigating water deficit damage in sugarcane

AbstractHigh root absorption of silicon (Si) in sugarcane plants that accumulate the element attenuates water deficit‐induced damage. However, there is a lack of research to identify whether the low relative absorption of Si via leaf spraying compared to root application has an immediate antioxidant effect on plants under water restriction. For this purpose, the objective of this research was to evaluate the effect of Si applied via leaf spraying and via nutritive solution (root) in mitigating the damage caused by short‐term water deficit induced by PEG‐6000 in pre‐sprouted sugarcane seedlings. The treatments were arranged in a 3 × 2 factorial scheme, consisting of the forms of Si supply: via nutrient solution (SiR) (2.0 mmol/L), via foliar spraying (SiL) (3.4 mmol/L) plus the absence of Si (−Si), combined with the presence (−0.6 MPa) and absence of water deficit, arranged in randomized blocks with 6 repetitions. The experiment was developed in a hydroponic system, in which Si was initially supplied during the seedling production phase; subsequently, water deficit was induced with PEG‐6000 for a period of 72 hr. Plants under water deficit increased the accumulation of Si with the supply of the element via root application and via foliar spraying by 2118% and 98%, respectively, in comparison to the absence of Si. However, although Si foliar absorption was relatively low in comparison to the root, the physiological effects in relieving water deficit‐induced stress were equal to or even higher than those of root application. In conclusion, the amount of Si absorbed by the plant is not a major factor to induce an antioxidant defence response to mitigate the immediate effects of water deficit, since the two forms of application of Si were similar. Si foliar spraying promotes physiological and biochemical effects that alleviate damage done water deficit short term, even on plants with an active absorption mechanism such as sugarcane.

Parasitism Behavior of Tetrastichus howardi (Hymenoptera: Eulophidae) on Larvae and Pupae of Sugarcane Borers

Control of the sugarcane borers, Diatraea saccharalis and Diatraea impersonatella (= D. flavipennella) (Lepidoptera: Crambidae), in Brazil, is based on mass release of the larval parasitoid Cotesia flavipes (Hymenoptera: Braconidae). However, this method does not prevent some pupae of these borers from completing development and recolonizing the crop. Thus, additional control is required. This study investigated the parasitoid Tetrastichus howardi (Hymenoptera: Eulophidae) to control pupae of sugarcane borers, especially D. impersonatella. Regardless of larval instar and species, no larval parasitism occurred within 24 h of exposure. Nevertheless, a longer exposure period (ca. 96 h) resulted in parasitism of older larvae. Approximately 90% of pupae of both borers were parasitized by T. howardi within 24 h of exposure, and the parasitoid produced a similar number of offspring with >90% females. The developmental time of T. howardi parasitizing D. impersonatella pupae was delayed ~2 days from that of in D. saccharalis. No-choice trails revealed that parasitoid females took longer to start probing larvae compared to pupae. Likewise, when larvae and pupae were offered simultaneously (choice test), the latter represented the first choice of about 92% T. howardi females. The elapsed time to first attack was less on pupae than on larvae, whereas the number of attacks and probing time was greater on pupae. In addition, parasitism on D. impersonatella pupae was higher than on larvae feeding on artificially infested sugarcane seedlings. These results suggest that T. howardi prefers to parasitize the pupal stage and has the potential to be used as a biological control agent to complement the control of sugarcane bores.

The influence of compost and arbuscular mycorrhizal fungi on sugarcane growth and nutrient uptake

Compost and Arbuscular mycorrhizal fungi are environmentally sustainable and low-cost materials that can benefit tropical soils with high phosphorus fixation and low organic matter content. This study investigated the effects of Arbuscular mycorrhizal fungi (AMF) and compost on the growth and nutrient uptake efficiency of sugarcane (Saccharum officinarum) seedlings. The experimental design was a completely randomized factorial design, where factor A (n = 5) was the compost doses (0, 15, 30, 60 and 120 t ha-1) and B (n = 3) the AMF inoculum (Rhizophagus clarus, Gigaspora margarita, and non-inoculated). At 30 and 90 d, seedlings’ diameter and height were measured. Mycorrhizal colonization rate, biomass production, nutrient uptake (P and N), and mycorrhizal dependency were assessed at the end of the experiment. The AMF and compost doses affected the colonization rate, initial growth, biomass production, and nutrient uptake of sugarcane seedlings. Overall, the AMF benefited plant growth at lower doses of compost. R. clarus had a higher impact on the shoot diameter of sugarcane seedlings. Mycorrhizal colonization increased with compost addition only in seedlings inoculated with G. margarita. There was no clear trend among AMF treatments for nutrient uptake. In general, sugarcane seedlings dependency on mycorrhizal condition to produce growth was higher at lower compost doses.

Bud chip method of sugarcane planting: A review

Sugarcane crop requires huge quantity of seed cane for planting under conventional method, which contributes a major share in cost of cultivation. Besides, large quantity of seed material poses a big challenge for transportation and handling. This problem can be effectively addressed through adoption of sustainable sugarcane initiative through planting of bud chips, which can save the cost and inconveniences associated with conventional planting methods. Several authors have reported advantages of planting single bud chips over conventional methods with respect to germination, crop establishment, growth and development of sugarcane crop. Sustainable sugarcane initiative technology favourably influenced various yield attributing factors such as plant stand, millable cane per clump and weight of single cane thereby resulting in higher yield. Some authors have also recorded higher brix value and higher juice weight at harvesting stage with planting of single chip bud seedlings of sugarcane. Compared with conventional method, economics of cultivation goes in favour of bud chip method of planting. Based on research findings by various workers, it can be said that planting of sugarcane by bud chip method is superior to planting by conventional methods.

Plant-Growth Endophytic Bacteria Improve Nutrient Use Efficiency and Modulate Foliar N-Metabolites in Sugarcane Seedling.

Beneficial plant–microbe interactions lead to physiological and biochemical changes that may result in plant-growth promotion. This study evaluated the effect of the interaction between sugarcane and endophytic bacterial strains on plant physiological and biochemical responses under two levels of nitrogen (N) fertilization. Six strains of endophytic bacteria, previously selected as plant growth-promoting bacteria (PGPB), were used to inoculate sugarcane mini stalks, with and without N fertilization. After 45 days, biomass production; shoot nutrient concentrations; foliar polyamine and free amino acid profiles; activities of nitrate reductase and glutamine synthase; and the relative transcript levels of the GS1, GS2, and SHR5 genes in sugarcane leaves were determined. All six endophytic strains promoted sugarcane growth, increasing shoot and root biomass, plant nutritional status, and the use efficiency of most nutrients. The inoculation-induced changes at the biochemical level altered the foliar free amino acid and polyamine profiles, mainly regarding the relative concentrations of citrulline, putrescine, glycine, alanine, glutamate, glutamine, proline, and aspartate. The transcription of GS1, GS2, and SHR5 was higher in the N fertilized seedlings, and almost not altered by endophytic bacterial strains. The endophytic strains promoted sugarcane seedlings growth mainly by improving nutrient efficiency. This improvement could not be explained by their ability to induce the production of amino acid and polyamine composts, or GS1, GS2, and SHR5, showing that complex interactions may be associated with enhancement of the sugarcane seedlings’ performance by endophytic bacteria. The strains demonstrated biotechnological potential for sugarcane seedling production.

Effect of Bacillus aryabhattai on the initial establishment of pre-sprouted seedlings of sugarcane varieties

This study proposes to examine the potential use of Bacillus aryabhattai in pre-sprouted seedlings of sugarcane inoculated with the microorganism when subjected to different regimes of water supply after transplanting. The experiment was laid out in a randomized-block design with a complete 3 × 2 × 5 factorial arrangement, in five replicates. The factors were represented by pre-sprouted seedlings of three sugarcane varieties (IAC 911099, RB 855156 and CTC 20), two seedling types (inoculated and not inoculated with B. aryabhattai) and five frequencies of water supply, which provided the ability of return to 100% soil field capacity, at every 0, 10, 20, 30 and 40 days. Plant mortality, plant height, number of leaves, stalk diameter and number of tillers were evaluated throughout the experimental period. At the end of the study, the shoot dry matter (SDM) and root dry matter (RDM) contents of the plants were measured. The number of leaves on the plant was affected only in isolation, according to the cultivar, without effects of the other studied factors. There was a double interaction effect between the factors of variety and inoculation (V*I) for the variables of SDM, stalk diameter and height; and between frequency and inoculation (F*I) for stalk diameter. There was a triple interaction effect between variety, inoculation and frequency (V*I*F) for the RDM variable. Thus, the use of B. aryabhattai as an inoculant in pre-sprouted seedlings of sugarcane at the time of seedling formation can improve plant development after transplanting depending on the cultivar used, especially in IAC 911099 and RB 855156.

Diazotrophic bacteria and substrates in the growth and nitrogen accumulation of sugarcane seedlings

Little is known about the interaction between the inoculation of diazotrophic bacteria, the variety, and the substrate used in inoculated sugarcane seedling production. Bearing this in mind, the aim of this study was to evaluate the effects of diazotrophic bacteria inoculation, four sugarcane varieties and four different substrates on the growth and nitrogen accumulation of sugarcane seedlings. Parameters related to sprouting, height, and root growth, as well as nitrogen accumulation, were evaluated. The results indicate that inoculating sugarcane seedlings belonging to the following varieties: RB867515, RB92579, RB966928 and RB975932 with bacteria may increase sprouting speed index, height, root length, fresh and dry matter weight, as well as nitrogen accumulation, which has resulted in a more uniform stem diameter. However, the responses to inoculation differ from variety to variety and are also dependent on the substrate used with better results observed in the commercial substrate and the substrate mixtures containing a higher proportion of organic compost. Understanding the interaction between the variety and the substrate with the bacterial inoculation is essential to the success of the production of inoculated sugarcane seedlings.

Multifunctional characteristics of Acinetobacter lwoffii Bac109 for growth promotion and colonization in micropropagated sugarcane

ABSTRACT Endophytic bacteria with multifunctional characteristics can benefit plants through different mechanisms, as well as promoting growth in an efficient, low-cost and ecofriendly way. This study analyzed the potential of the multifunctional endophytic isolate Acinetobacter lwoffii Bac109 in promoting the early in vitro growth of sugarcane seedlings. The Bac109 strain showed potential to solubilize phosphate in a solid medium (solubilization index: 3.73). In addition, the bacterium was an efficient biocontrol agent against the phytopathogenic fungi Rhizoctonia sp., Fusarium oxysporum, Phoma sp. and Bipolaris papendorfii, showing a performance equal to or better than the commercial antifungal hygromycin B. An in vitro assay confirmed the biofilm production, which increased in the presence of sugarcane root extract. Additionally, A. lwoffii Bac109 showed a strong adhesion to the sugarcane roots. The inoculation of this bacterium in micropropagated sugarcane seedlings increased the shoot length (35 %) and regulated the nonphotochemical energy dissipation after 28 days of cultivation. At the end of the experiment, the bacterium showed a great potential for survival, with 5.72 × 107 CFU mL-1 recovered from the substrate, what is crucial for plant interaction. The results showed the potential of the biotechnology application for A. lwoffii Bac109 by evaluating multifunctional traits of plant growth promotion and by specific interactions with sugarcane, which may help to improve micropropagation protocols for this crop.

Sugarcane pre-sprouted seedlings produced with beneficial bacteria and arbuscular mycorrhizal fungi

ABSTRACT The demand for the increased productivity of sugarcane crops has required changes in its production chain, such as the use of pre-sprouted seedlings (PSS) of sugarcane into the chain. In this system, the inoculation of beneficial microorganisms, such as plant growth-promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF), can improve seedling development. The objective was to evaluate the beneficial effect of PGPB and AMF inoculation on sugarcane PSS production system. Experiments were carried out in greenhouse using a commercial substrate with different levels of fertilization to evaluate the plant biomass and nutritional status. The bacteria strains are able to produce indole acetic acid and to amplify the nifH gene. The coinoculation of strains IAC-BeCa-095 with AMF (Glomus macrocarpum and Glomus etunicatum) improved the plant shoot biomass (25%) on the fertilized substrate. The strains IAC-BeCa-088 (Burkholderia caribensis), IAC-RBca5 (Pseudomonas sp.) and IAC-RBca10 (Bacillus sp.) without AMF and fertilization improved the shoot biomass by up to 35%. Coinoculation with strain IAC-BeCa-095 (Kosakonia radicincitans) and AMF improved the shoot and root biomass by up to 27 and 75%, respectively, in the conventionally fertilized substrate, demonstrating a synergistic effect of these microorganism consortia. The use of beneficial microorganisms may be a viable practice in the production of PSS sugarcane. Moreover, this study is the first to demonstrate the synergistic effect of endophytic bacteria (K. radicincitans) or rhizobacteria (Bacillus sp.) with AMF and Pseudomonas sp. or B. caribensis, without AMF inoculum on the production of sugarcane PSS to improve plant growth and plant nutrition.

Quality of pre-sprouted sugarcane seedlings grown on substrates with decomposed babassu palm stem

ABSTRACT The use of pre-sprouted seedlings in the implantation of sugarcane crops not only reduces the establishment period and production costs, but also has other advantages, such as a lesser requirement of propagules (stems) and greater phytosanitary control of the seedlings, when compared to conventional planting. Commercial substrates are also expensive and their use increases the cost of seedlings. This study aimed to evaluate the quality of sugarcane pre-sprouted seedlings grown on substrates with different proportions of decomposed babassu palm stem (DBPS), as well as to recommend the substrate formulation that provides the most favorable environment for the growth and development of sugarcane pre-sprouted seedlings. For preparation of the substrate (S), DBPS and soil were used in the following proportions: S1: 0 % of DBPS + 100 % of soil; S2: 20 % of DBPS + 80 % of soil; S3: 40 % of DBPS + 60 % of soil; S4: 60 % of DBPS + 40 % of soil; S5: 80 % of DBPS + 20 % of soil; S6: 100 % of DBPS + 0 % of soil. The use of S2 and S3 considerably improved the seedling quality parameters, such as sprout diameter and length, number of leaves and total, shoot and root dry mass. However, from an economic point of view, S2 is recommended for use.

Antioxidative metabolism in sugarcane (Poaceae) varieties subjected to water and saline stress

ABSTRACT This study aimed to identify the antioxidant responses of sugarcane (Saccharum spp.) varieties subjected to water and saline stress. Sugarcane seedlings of six different varieties obtained through micropropagation were subjected to either water or saline stress, or a combination of water + saline stress. The study was carried out in May 2012, in a greenhouse at the Universidade Federal Rural de Pernambuco (UFRPE). The experimental design was randomized, with treatments arranged in a 6 × 4 factorial scheme (six varieties and four treatments), and four replicates. Lipid peroxidation, hydrogen peroxide (H2O2) concentrations, and relative water content (RWC) were evaluated. Furthermore, we evaluated the plants’ antioxidative defense systems by measuring the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT). The sugarcane varieties had higher lipid peroxidation and/or higher H2O2 concentrations when subjected to the combined water + saline stress. The antioxidant enzymes responded to the water and saline stress treatments differently depending on the sugarcane variety. However, under combined saline + water stress conditions, the enzymes may have become inactivated, which indicates that the response to the combined water + saline stress was different from the sum of the responses to only water stress or only saline stress. High concentrations of malondialdehyde (MDA) associated with low RWC may be an effective indicator of multiple stress sensitivity in sugarcane varieties. The RB99395 and RB867515 sugarcane varieties responded more efficiently to environmental stress, and maintained their cell water content when subjected to either water or saline stress.