Sugarcane pre-sprouted seedlings produced with beneficial bacteria and arbuscular mycorrhizal fungi

Lenise Rossetto,Gabrielle Maria Fonseca Pierangeli,Eiko Eurya Kuramae,Matheus Aparecido Pereira Cipriano,Mauro Alexandre Xavier,Adriana Parada Dias Da Silveira

doi:10.1590/1678-4499.20200276

Lenise Rossetto, Gabrielle Maria Fonseca Pierangeli + Show 4 more

Open Access

https://doi.org/10.1590/1678-4499.20200276

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Abstract

ABSTRACT The demand for the increased productivity of sugarcane crops has required changes in its production chain, such as the use of pre-sprouted seedlings (PSS) of sugarcane into the chain. In this system, the inoculation of beneficial microorganisms, such as plant growth-promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF), can improve seedling development. The objective was to evaluate the beneficial effect of PGPB and AMF inoculation on sugarcane PSS production system. Experiments were carried out in greenhouse using a commercial substrate with different levels of fertilization to evaluate the plant biomass and nutritional status. The bacteria strains are able to produce indole acetic acid and to amplify the nifH gene. The coinoculation of strains IAC-BeCa-095 with AMF (Glomus macrocarpum and Glomus etunicatum) improved the plant shoot biomass (25%) on the fertilized substrate. The strains IAC-BeCa-088 (Burkholderia caribensis), IAC-RBca5 (Pseudomonas sp.) and IAC-RBca10 (Bacillus sp.) without AMF and fertilization improved the shoot biomass by up to 35%. Coinoculation with strain IAC-BeCa-095 (Kosakonia radicincitans) and AMF improved the shoot and root biomass by up to 27 and 75%, respectively, in the conventionally fertilized substrate, demonstrating a synergistic effect of these microorganism consortia. The use of beneficial microorganisms may be a viable practice in the production of PSS sugarcane. Moreover, this study is the first to demonstrate the synergistic effect of endophytic bacteria (K. radicincitans) or rhizobacteria (Bacillus sp.) with AMF and Pseudomonas sp. or B. caribensis, without AMF inoculum on the production of sugarcane PSS to improve plant growth and plant nutrition.

Highlights

Beneficial microorganisms, such as plant growth-promoting bacteria (PGPB) and mycorrhizal fungi, have been explored worldwide to enhance plant performance (Bhardwaj et al 2014; Venturi and Keel 2016)
This study is the first to demonstrate the synergistic effect of endophytic bacteria (K. radicincitans) or rhizobacteria (Bacillus sp.) with arbuscular mycorrhizal fungi (AMF) and Pseudomonas sp. or B. caribensis, without AMF inoculum on the production of sugarcane pre-sprouted seedlings (PSS) to improve plant growth and plant nutrition
The bacterial and arbuscular mycorrhizal fungal strains tested, which belong to the Beneficial Microorganisms Collection of Agronomic Institute, IAC, were: IAC-BeCa-088 (Burkholderia caribensis), IAC-BeCa-095 (Kosakonia radicincitans), Sugarcane seedlings production with plant growthpromoting bacteria (PGPB) and AMF

Summary

Introduction

Beneficial microorganisms, such as plant growth-promoting bacteria (PGPB) and mycorrhizal fungi, have been explored worldwide to enhance plant performance (Bhardwaj et al 2014; Venturi and Keel 2016). The plant microbiome harbors these beneficial microorganisms, which include different endophytic bacteria, such as Burkholderia, Herbaspirillum and Kosakonia, as well as those bacteria that inhabit the soil rhizosphere, recognized as plant growth-promoting rhizobacteria (PGPR), such as Azospirillum, Bacillus and Pseudomonas (Cipriano et al 2016; Meena et al 2017; Lata et al 2018). The use of these bacteria is a growing and welcome eco-friendly technology in sustainable agriculture. The benefit of mycorrhizal symbiosis represents an underexploited application to increase plant health and global food security (Rodriguez and Sanders 2015)

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