The effect of the biostimulant was studied on the yield and quality of the Sharad Seedless grape variety. Biostimulant i.e. Super Shakti Combine was sprayed at 3-4 mm and 6-8 mm berry size @ 500 ml per ha; 750 ml per ha; 1000 ml per ha; 1240 ml per ha; 1000 ml per ha (at 3-4 mm berry size), 1000 ml per ha (at 6-8 mm berry size) and control. Among the different treatments, application of 1250 ml per ha exhibited a significant increase in bunch weight (439.2 g), berry weight (5.78 g), 50-berry weight (286.2 g), berry length (25.5 mm), berry diameter (21.6 mm) followed by 1000 ml per ha (432.8 g, 5.59 g, 282.6 g, 25.1 mm, 20.7 mm), respectively. The highest yield (14.02 kg per vine) was recorded in treatment 1250 ml per ha at per with treatment 1000 ml per ha (13.75 kg per vine) with minimum physiological loss in weight during the storage. Thus the application of biostimulant i.e., Super Shakti Combine is found suitable to improve the yield and quality of grapes cv. Sharad Seedless.