Effects of parental genotypes on embryo rescue of seedless grape hybrid

Abstract

ABSTRACT The success of seedless grape embryo rescue depends on many factors. To study the effects of parental genotypes on embryo rescue, we conducted 11 hybridisation combinations using nine stenospermocarpic seedless grapes as female parents and three seedless or seeded grapes as male parents. At the optimal sampling time of each mother plant, the hybrid ovules were inoculated and cultured on a solid–liquid biphasic MM3 medium. Then, the bare embryos were removed and inoculated on a WPM medium for germination and seedling formation. The results showed that when ‘Jupiter’ (seedless) was the male parent, ‘Perlette’ had the highest rates of embryo development, germination and seedling formation, followed by ‘Kunxiang Seedless’; when ‘Su-67’ (seedless) was the male parent, ‘FZ42’ had the highest embryo development rate; when ‘Beichun’ (seeded) was the male parent, ‘Fresno Seedless’ had the best embryo rescue results, followed by ‘Qinhong No.10’. These suggest that ‘Perlette’, ‘Kunxiang Seedless’, ‘Fresno Seedless’ and ‘Qinhong No.10’ are suitable female parents for embryo rescue. When ‘FZ42’ was the female parent, ‘Su-67’ was better than ‘Jupiter’ as the male parent; when ‘Perlette’ was the female parent, ‘Jupiter’ and ‘Su-67’ as male parents had no significant difference, indicating that paternal genotype has a certain effect on embryo rescue. HIGHLIGHTS The maternal genotype has a significant effect on the embryo rescue of seedless grapes. The paternal genotype has a certain effect on embryo rescue of seedless grapes. ‘Perlette’, ‘Kunxiang Seedless’, ‘Fresno Seedless’ and ‘Qinhong No.10’ are suitable female parents. A total of 345 hybrids from 11 cross-combinations were obtained by embryo rescue.