Lots Of Seeds Research Articles

EFFECT OF PACLOBUTRAZOL ON THE GROWTH OF POTATO (Solanum tuberosum L.)

Low potato production in Indonesia is caused by poor seed quality and low availability of potato seeds. One of the efforts to produce superior and quality potato seeds is to apply growth regulators (ZPT) because they can produce a lot of potato seeds, but plant growth becomes depressed. The purpose of this study was to determine the effect of paclobutrazol concentration and application time on the growth of potato G2 (Solanum tuberosum L.). This research was carried outon UPT land. BIH Kutagadung Berastagi, which lasted for four months, from November 2020 to March 2021. The design used in this study was a Factorial Randomized Block Design consisting of 2 factors and repeated 3 times. The first factor is the concentration of the hormone Paclobutrazol (P) which consists of 4 levels, namely: P0 = Control; P1 = 200 ppm; P2 = 400 ppm; P3 = 600 ppm. The second factor was the application time of Paclobutrazol (W) which consisted of 3 levels, namely: W1 = 20 days after planting; W2 = 30 days after planting; W3 = 40 days after planting.The research results show thattreatment of paclobutrazol concentration had no significant effect on the number of leaves and stem diameter, but had a very significant effect on plant height,The time of application of paclobutrazol had no significant effect only on the number of leaves,but have a real effect onplant height, stem diameter,The interaction between concentration and application time of paclobutrazol had no significant effect on leaf number and plant height, but had a very significant effect on stem diameter..

A Review of the Establishment of the Seed Lot System in the Production of Biological Products and Its Importance.

Today, due to the importance of diseases controlled by vaccination, the production of biological products is of great importance in ensuring public health, so producing high-quality biological products plays an important role in maintaining public health. One of the most important principles of manufacturing high-quality and efficient biological products is using suitable seeds based on standard principles. To produce a suitable seed for continuous use for mass production of biological products, the seed must be defined according to certain principles and foundations. These principles are formed in the form of a seed lot system. In this review article, all the requirements for the establishment of a seed to produce a biological product include general seed information, including basic and seed information and microorganisms, seed-specific information including seed passage levels, propagation method, seed storage conditions, coding and labeling, identification information and design of suitable laboratories for passage, seed propagation, and storage, determination of seed characteristics including all necessary tests to determine seed identity, purity, potency, efficacy, stability, safety and also all the necessary information for documenting and storing seeds has been studied. Also, this study discusses the method of preparing all the necessary information for establishing a seed lot system, especially determining the characteristics of seeds. Based on the study's results, a complete and comprehensive seed lot system has been formed that can be used to prepare, propagate, passage, and store seeds used in the production of biological products.

Estimation of Seed Longevity in Onion Seed Lots by a Vigor Test of Radicle Emergence Test in Artificial Ageing Conditions

The study was conducted to test whether a vigor test of a single count of radicle emergence (RE test) would correlate with the storage potential of 10 seed lots of onion (Allium cepa L.) cultivars in artificial storage conditions. The RE test was performed by counting radicle emergence (2 mm) percentage at 80 h after germination was set up at 20 °C. Seed longevity was determined by storage at 75% relative humidity, obtained by saturated NaCl, at 35 °C over 60 days. Twelve seed samples were taken out during ageing, and seed survival curves were constructed based on normal germination percentages conducted at 20 °C for 12 days in the dark. The seed longevity criterion was P50 (time for the viability to fall to 50%), which was determined through probit analysis by using survival curves. Correlation analysis showed that RE counts at 80 h during germination were highly correlated (p < 0.001) with initial seed quality, Ki (R2 = 0.888) and the half-viability period, P50 (R2 = 0.823). Non-aged pre-storage final normal germination percentages of seed lots were also significantly related to longevity (Ki, R2 = 0.808, p < 0.001, P50, R2 = 0.750, p <0.01), while the level of significance was lower. It can be concluded that the RE test can be used as a fast vigor test to estimate the longevity in onion seed lots.

Seed Priming and Pericarp Removal Improve Germination in Low-Germinating Seed Lots of Industrial Hemp

Industrial hemp (Cannabis sativa L.) is experiencing a resurgence in North America as an agricultural commodity. Germplasm improvement for locally adapted and stable cannabinoid production is an industry priority. This study used seed priming and pericarp removal to recover seedlings in low-germinating seed lots that could prove important for plant breeding and germplasm conservation. Both seed priming and pericarp removal improved early seed germination, but pericarp removal was more effective in improving overall final germination percentages. On average, pericarp removal improved final germination in low-germinating seed lots by approximately 38% compared to intact seed germination. In seeds with the pericarp removed, the initial germination substrate had an impact on normal seedling development following transplanting: those germinated for 2 to 3 days on rolled towels produced more normal seedlings compared to those started in Petri dishes. There was a dramatic increase in abnormal seedlings produced in the low-germinating seed lot initially germinated in a Petri dish wetted with 6 mL water where nearly 80% never transitioned to normal actively growing seedlings.

Detection of Potato Mop-Top Virus in Potato Seed Lots Entering Washington State

Detection of Potato Mop-Top Virus in Potato Seed Lots Entering Washington State

Crossing of Potato in Tajikistan

Potato tubers are valuable food products for most people in the world, and in many regions are considered a “second bread”. The agricultural environment of mountainous areas of Tajikistan situated at an altitude of more than 1800 meters above sea level, make it possible to grow good and quality harvest of potato. Under these cool mountainous conditions, potato plants are of little affection, have intensive bloom, and form a lot of berries and botanic seeds.

Development of an ethylometer with an MQ-3 sensor for measuring ethanol in soybean seeds

ABSTRACT In the seed production sector, it is essential to use fast and practical equipment to help decision-making processes. Thus, the use of sensors that can capture information to assist in agriculture has contributed significantly to soybean cultivation worldwide, being used in several stages, whether pre-harvest or post-harvest. Thus, this study aimed to develop a prototype of ethylometer with an MQ-3 sensor and to evaluate its stabilization time when used in soybean seed lots submitted to hypoxia. The research was carried out with 10 lots of soybean seeds from five cultivars (NS 8383 RR, SOY Peso RR, M 8372 IPRO, M8644 IPRO and M8808 IPRO) and was divided into two stages. The first one corresponds to the development of the ethanol measuring equipment with an MQ-3 sensor, and the second one to measurement of the sensor’s stabilization time (3, 6, 9, 12, 15, 18, 21, 24, 27, and 30 s) in soybean seeds submitted to anaerobiosis. The equipment and the MQ-3 sensor showed the stability of average measurement at 16 s after activation. This time is recommended for use of the ethylometer in soybean seeds. Through the MQ-3 sensor, it is possible to efficiently detect ethanol present in the air released by soy seeds during the anaerobic process.

Responses of seed vigour and germination of maize to plasma-activated water priming, silver-nanopriming and green silver-nanopriming

The application of atmospheric non-thermal plasmas and nanomaterials for seed technology is relatively new compared to their use in industrial and agricultural sectors. This study aimed to investigate the potential of plasma-activated water (PAW) priming, silver-nanopriming and green silver-nanopriming for seed performance enhancement in maize. The experiment was carried out using two lots of maize seeds with a one-year difference in age. Seed treatment procedures were priming with PAW formular 1 (PAW1), with silver nanoparticles (AgNPs) using PAW formular 1 (PAW1SNP), with green-AgNPs using PAW formular 1 (PAW1GSNP), with PAW formular 2 (PAW2), with AgNPs using PAW formular 2 (PAW2SNP), with green-AgNPs using PAW formular 2 (PAW2GSNP) and hydropriming with deionised water; untreated seeds were used as the control. Results showed that the best protocol for enhancing seed vigour and germination performance of maize was PAW2. Priming with PAWs, PAW1 or PAW2, has the potential to enhance the physiological quality of maize seeds, particularly new seeds. The limitations of PAW1SNP, PAW1GSNP, PAW2SNP and PAW2GSNP in seed enhancement and storability after priming treatments are discussed.

Supply Chain Analysis of Green Mussel Business in Umbul Asem Village Bandar Lampung

Umbul Asem village is a coastal area has a fairly high potential, especially the farming of green mussels. However, the prospect of green mussel farming which is quite large is still not realized by all fishermen so it is necessary to conduct research to determine the green mussel supply chain and see what opportunities from its business so that it can be developed. This is a descriptive research which is conducted in field and desk research. The results indicate the green mussel supply chain starts from farmers to distributors then to retailers and to end consumers, while supply chain players manage relationships between supply chain from upstream to downstream and vice versa well. Some of the green mussel business opportunities that are visible include selecting the Longline method to minimize costs and increase profits also suitable for high wave waters, farming is simple and does not require special treatment, lots of green mussel seeds are available in the waters, the profit from one longline is around Rp. 691,667, the location is close to PPI Lempasing (3 km in 8 minutes). In addition to developing the green mussel business, it is necessary to conduct a study that is in line with the government's efforts in formulating a strategy for developing the fishing industry in Bandar Lampung.

Matriconditioning effect on the physiological performance of chia seeds (Salvia hispanica L.)

Solid matrix priming (matriconditioning) permits slow seed hydration, and germination and emergence period reduction. In general, seeds need to be soaked to start their germination process. The objective of this study was to evaluate the effect of the matriconditioning under the imbibition curve and the physiological performance of two lots of chia seeds with different qualities. The study was performed with chia seeds harvested by small-scale farmers. To estimate the imbibition curve, seeds from both lots were started in the imbibition process on a stainless-steel screen placed in plastic boxes, containing 55.5 grams of sterilized vermiculite and moisturized with distilled water at 100% of their retention capability. The imbibition curve weights were made 0, 2, 4, 8, 16, and 32 hours after the process had started. Simultaneously, in each evaluation period, seeds were submitted to the following tests: germination, first count of germination, plantlet shoot, root and total lengths, emergence velocity index and average time, and accumulated emergence. A completely randomized experimental design was used. It was possible to conclude that the beginning of phase II of germination in matriconditioned seeds varies in function of the chia seeds' lot quality. The matriconditioning contributes to the physiological performance, increasing and accelerating plantlets' emergence.

Protein profile in arabica coffee seeds in electrophoresis gel: importance of freeze-drying

Abstract: Coffee seeds are sensitive to desiccation and are used or stored with different moisture content values, which may affect the results of quality assessment. The aim of this study was to evaluate the changes in protein profile in electrophoresis gel in coffee seeds with different moisture content values under freeze-drying and without freeze-drying. Two lots of arabica coffee seeds were used, one of newly-harvested seeds and another of stored seeds. The seeds were dried to the moisture content values of 12, 15, 20, 25, 30, 35, and 40%. The physiological quality of the seeds was assessed through the germination test, electrical conductivity, and the profiles of the enzymes SOD, CAT, PO, GOT, MDH, and EST and of heat-resistant proteins. In general, there is an effect on expression of these enzymes in accordance with the presence of free water in the seeds. Moist seeds have little to no enzyme expression. The freeze-drying process allows preservation of coffee seed quality and does not change the functionality of the enzymes studied. The isoenzyme profiles of the antioxidant process in arabica coffee seeds are affected by the initial moisture content of the seeds. The freeze-drying process of the seeds ensures greater sensitivity in detection of the expression of these isoenzymes.

Ethanol and hormones in physiological conditioning on germination and seed dormancy of Urochloa humidicola cv. Llanero

Abstract: Seed conditioning improves germination uniformity, speed, and dormancy release. This work aimed the study the physiological conditioning of Urochloa humidicola cv. Llanero, using two lots of dormant seeds conditioned by imbibition with water, commercial bioregulator (auxin, gibberellin - 0.144 mM and cytokinin), gibberellin (0.144 and 1.44 mM) or 5% ethanol. Germination, dormancy, SOD activity, MDA and H2O2 content were evaluated. In both lots, germination percentage was positively influenced by the commercial bioregulator without affecting the viability. In the first, the germination, using a bioregulator or ethanol, was higher; in the second, the highest germination was from the treated seeds concerning the control and water treatment. The unconditioned seeds presented a higher H2O2 and protein content in both lots. The MDA content in the first lot was higher in the non-conditioned seeds and conditioned with ethanol. In the second lot, the water conditioned seeds had higher content. In both lots, the bioregulator conditioned seeds showed higher SOD activity. The H2O2 content was related to antioxidation activation. It was impossible to identify the oxidative window of germination in which H2O2 would be a signal, releasing germination. The conditioning of seeds with any product showed superior physiological quality than the unconditioned or conditioned with water.

Chemical composition and great applications to the fruit of the pomegranate (Punica granatum): a review

The pomegranate (Punica granatum) belong the Punicaceas family and is a plant of Iran that is characterized by the fruit that it give because is a big globular berry that inside have a lot of seeds wrapped in a juicy edible pulp covered for a gross peel with green, yellow and red tonalities depending of the ripening state and the varieties. The harvest and consumption of the fruit has gone increasing in Mexico due of the actual findings of phytochemical compounds whit antioxidant, anti-inflammatory and antiviral activity with application in products of food industry and pharmaceutical and it has even been observed microbicide activity versus fungus responsible of crop rot and pathogenic bacteria, nevertheless, in the treatment of the fruit the peel isn´t used and is discarded, so, in this project we want to describe on general way the fruit and the alternatives that has been worked actually in the exploration and application of phytochemical compounds like the polyphenols in whole the plant.

Physical characterization ex-situ reproduction of Chamaedorea metallica non-timber forest resource

Seed quality analysis determines factors that can affect a seed lot or samples. The aim of the studio was determine physical qualityof a lot of seeds of the Chamaedorea metallica collected in two fruiting periods; March to September 2018 and February to April2019; They were collected from mother plants of Chamaedorea metallica in the Ye`tsil garden at the Technological Institute of theValley of Oaxaca. The fruits presented purple-purple coloration in the exocarp; they were cleaning process to obtain the seeds,which consisted of soaking the fruits in water for 48 hours, they washed with running water until the mesocarp was completelydetached. The seeds dried under shade for five days on bond paper. The analysis carried out according to the minimum parametersmentioned by the International Seed Testing Association (ISTA). Data of percentage of purity, weight, number of seeds per kgandmoisture content obtained. The data from the physical analysis differed in both years, obtaining the best results in moisture contentand weight in the seeds collected in 2019. We concluded that the seeds of the mother plants of Chamaedorea metallica are of goodquality; that the collection period and the time of the fruit on the plant influence their quality.

Penggunaan Air Kelapa Muda Sebagai Zat Pengatur Tumbuh Terhadap Daya Kecambah, Vigoritas, Berat Kering Biji Tanaman Kelor (Moringa oleifera)

The more common methode for planting moringa plant is using seed, because this plant produces a lot of seeds. However, the resulting seeds have a hard enaugh seed coat so that. These conditions can affect and even thwart germination, vigor and growth of the plant. This Study aims to determine the proper dosage of young coconut water to be used in growing moringa seeds, so that later it become a recommendation for formers in the development of moringa plants. This research was conducted using a green house and laboratory scale method with the media that had been prepared for germination obsevations were carried out for two weeks to obtain data according to the observed parameters which included capacity, Vigority and dryweight of moringa plants. The result of the analysis of variety showed that the concentration of young coconut water had no significant effect (P>0.05) on the germination, vigor and dryweight of moringa plants. Likewise, the interaction between immersion time and coconut water concentration had no significant effect (P>0.05) on the observed variables. This situation is due to the fact that not all moringa seeds undergo the imbibition process properly so that they effect the observed variables.

Vigor and alpha-amylase activity in common bean seeds under salt stress conditions

Seeds with high vigor have greater capacity for hydrolysis and mobilization of stored reserves, which results in the formation of vigorous seedlings, and this behavior is observed under abiotic stress conditions. This study proposes to investigate the relationship of the enzyme alpha-amylase in lots of common-bean seeds with contrasting vigor, when subjected to the absence and presence of salt stress, aiming to identify the relationship of this enzyme with the vigor of the seed lot under these conditions. Seven common-bean cultivars were used. Physiological quality was determined by germination, vigor index and seedling length. The mobilization of reserves was evaluated under absence and presence of salt stress simulated with a NaCl solution with a concentration of 50 mmol L-1. The analyzed variables regarding reserve mobilization were reserve reduction, reserve reduction rate, seedling dry weight, reserve mobilization rate, starch, starch reduction rate and alpha-amylase activity. Results showed that the stress condition negatively affected all the evaluated variables; however, the cultivars classified as having greater vigor showed better physiological performance under stress. Salt stress in common-bean seeds affects seedling performance and reduces alpha-amylase activity during germination, and high-vigor seed lots exhibited higher enzyme activity in the no-stress condition.

​A Novel Seed Priming Technique for Enhancing Seed Vigour and Yield Potential in Marginal Vigour Seeds of Blackgram (Vigna mungo L.)

Background: Seed priming is controlled hydration and dehydration that results in improved seed quality and yield. However, hydration-dehydration of fresh seeds would result in soaking injury especially in legumes. The disadvantage reported in pulses can overcome through pre-conditioning of seeds which allows the seeds to slow hydration and prevent the soaking injury. The conditioning of seeds in large scale is tedious and skill oriented. To overcome this problem, an innovative seed priming technology is essential for pulses to enhance the seed germination, vigour and seed yield. Methods: Experiment was carried out at National Pulses Research Centre, Vamban during 2016-18. Seed priming technology was developed by using marginal vigour lot of blackgram. The marginal vigour seeds were packed in gunny bag up to 3/4th level and soaked in water for 2 h and 4 h and incubated for 2 h, 4 h and 6 h. After the incubation period, seeds were dried back to original moisture content (9.0%) and seed quality parameters were analyzed. The best performing organic priming concentrations under laboratory were forwarded to field trial and yield parameters were assessed. Result: Marginal vigour lot of blackgram seeds packed in gunny bag and soaked in seaweed extract @ 0.4% for 2 h and incubated for 4 h significantly increased the seed quality parameters. Among the various organic priming, marginal vigour lot of blackgram seeds packed in gunny bag and soaked in seaweed extract @ 0.4% for 2 h and incubated for 4 h significantly increased the seed quality parameters and yield over hydro priming and control.

​Seed Replacement Rate and Management of Wheat Seed in Amritsar District of Punjab

Background: Seed is the starting point of agriculture and dictates ultimate productivity of other inputs. Quality seed of improved varieties is an important basic unit for enhancing productivity of any crop species. As the quality deteriorates during subsequent generations, the old must be replaced with fresh lots of quality seeds. The present study was an attempt to analyze the source, SRR and management of wheat seed in Amritsar district of Punjab. Methods: The current study was conducted on primary data collected from 120 farmers during the agricultural year 2019-20. The data was analyzed using simple tabular analysis and other suitable statistical techniques. Result: The study found that the most preferred source of seed was institutional sources from which 45.83 per cent of the seed was procured from these agencies. The second most preferred source was self-retained by the farmers as 43.70 per cent farmer’s preferred self-retained seeds. Only small farmers and medium farmers procured wheat seed from fellow farmers, relatives and friends. This source was preferred by small and medium farmers as seed is available free of cost and no immediate cash payment was charged by fellow farmers or relatives. Seed replacement rate was found to be 49.94 per cent for wheat crop in Amritsar district of Punjab. The study found that minimum attention was paid at post storage of wheat seed (2.50 per cent) whereas maximum attention was paid in the field (48.83 per cent) during wheat seed production. The main determinants of purity and quality of wheat seed were past experience of the farmer, repute of the institution, tag of certification agency and advice of known person. Seed replacement rate (SSR) for wheat was less for small farmers in the study area which can be increased by giving incentives to farmers for using certified seeds. These incentives can be in the form of higher price of produce to the small farmers. Maximum farmers used self-retained seed foe the next season and they paid minimum attention to the storage of seed which lead to deterioration in the quality of seed. Farmers should be educated to use scientific methods in the storage of seeds.

PHYSIOLOGICAL QUALITY AND ANTIOXIDANT ENZYMATIC ACTION IN SUNFLOWER SEEDS EXPOSED TO DETERIORATION1

ABSTRACT This work aimed to evaluate the physiological and biochemical changes resulting from controlled deterioration in different lots of sunflower seeds. Two sunflower seed lots of the cultivar Hélio 253 were subjected to simulated deterioration, using the accelerated aging test methodology (41 °C and 100% RH) for 0, 48, 72, and 96 h. Then, the seeds were subjected to the tests of germination, first germination count, seedling length and dry matter, emergence, accelerated aging, electrical conductivity, and total seedling length. The antioxidant activity was evaluated through the enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX), and peroxidase (POX) at 0, 2, 4, and 6 days after sowing. Deterioration caused a reduction in the germination and vigor of the seeds, mainly in the lot with lower initial vigor and for the longest exposure times (72 and 96 h). For both lots, reductions in SOD activity and increases in POX and APX were observed during seed germination, mainly after 48 h of exposure to aging deterioration. Peroxidase enzymes are activated in deteriorated and non-deteriorated sunflower seeds, mainly after two days of germination.

Comparison of lots of pea seeds produced in northwest of Paraná

The establishment of the crop demands the use of seeds that allow the plants to perform better in adverse conditions. The study aimed to compare lots of pea seeds produced in the northwestern region of Parana. The experiment was carried out in a completely randomized design with seven seed lots and four replications. The seeds were obtained in field production, with similar management conditions. The determination of the weight of a thousand seeds, water content, electrical conductivity, percentage of germination, fresh and dry mass of the aerial part and root of the seedlings performed. The data were subjected to analysis of variance and the means compared by the Tukey test with 5% significance. The physiological potential not directly related to the seed mass; of the pea lots produced under adverse conditions, lots L3 and L4 showed superior quality. The physiological tests of electrical conductivity and germination differentiated the quality of the seeds. According to the selection criteria, the seeds of four pea lots showed germination higher than 85%, being the seeds of the lot L3 and L4 presented superior quality.